Craig Roy, PhD
Waldemar Von Zedtwitz Professor of Microbial Pathogenesis and of Immunobiology and Director of Biological and Biomedical Sciences (BBS)DownloadHi-Res Photo
Cards
Appointments
Microbial Pathogenesis
Primary
Immunobiology
Secondary
Additional Titles
Vice-Chair, Department of Microbial Pathogenesis
Co-Director of Graduate Admissions (DGA), Microbiology PhD Program of Biological and Biomedical Sciences
Contact Info
Microbial Pathogenesis
Department of Microbial Pathogenesis, 295 Congress Avenue, BCMM 354B
New Haven, CT 06536-0812
United States
Appointments
Microbial Pathogenesis
Primary
Immunobiology
Secondary
Additional Titles
Vice-Chair, Department of Microbial Pathogenesis
Co-Director of Graduate Admissions (DGA), Microbiology PhD Program of Biological and Biomedical Sciences
Contact Info
Microbial Pathogenesis
Department of Microbial Pathogenesis, 295 Congress Avenue, BCMM 354B
New Haven, CT 06536-0812
United States
Appointments
Microbial Pathogenesis
Primary
Immunobiology
Secondary
Additional Titles
Vice-Chair, Department of Microbial Pathogenesis
Co-Director of Graduate Admissions (DGA), Microbiology PhD Program of Biological and Biomedical Sciences
Contact Info
Microbial Pathogenesis
Department of Microbial Pathogenesis, 295 Congress Avenue, BCMM 354B
New Haven, CT 06536-0812
United States
About
Titles
Waldemar Von Zedtwitz Professor of Microbial Pathogenesis and of Immunobiology and Director of Biological and Biomedical Sciences (BBS)
Vice-Chair, Department of Microbial Pathogenesis; Co-Director of Graduate Admissions (DGA), Microbiology PhD Program of Biological and Biomedical Sciences
Biography
Craig Roy received his B.S. from Michigan State University in 1985 and earned his Ph.D. in Microbiology and Immunology at Stanford University in 1991 in the laboratory of Dr. Stanley Falkow. After completing a postdoctoral fellowship with Dr. Ralph Isberg in the Department of Molecular Microbiology at Tufts University School of Medicine in 1996, he was appointed as an Assistant Professor in the Department of Molecular Genetics and Microbiology at Stony Brook University. Dr. Roy became a founding member of the Department of Microbial Pathogenesis at Yale University in 1998 and serves as Vice-Chair. He currently holds the title of Waldemar Von Zedtwitz Professor of Microbial Pathogenesis and Immunobiology. Research in the Roy laboratory focuses on the host-pathogen interface. Using multi-disciplinary approaches his laboratory has discovered many novel mechanisms that intracellular pathogens use to modulate host membrane transport pathways, which allow these pathogens to evade cell autonomous defenses and create novel organelles that permit bacterial replication.
Appointments
Microbial Pathogenesis
ProfessorPrimaryImmunobiology
ProfessorSecondary
Other Departments & Organizations
Education & Training
- PhD
- Stanford University (1991)
Research
Overview
Medical Research Interests
Bacteria; Coxiella burnetii; Immune System; Legionella pneumophila; Macrophages; Protozoan Proteins; Vesicular Transport Proteins
ORCID
0000-0003-4490-440X
Research at a Glance
Yale Co-Authors
Frequent collaborators of Craig Roy's published research.
Publications Timeline
A big-picture view of Craig Roy's research output by year.
Research Interests
Research topics Craig Roy is interested in exploring.
Jun Liu, PhD
Samuel Steiner, PhD
Derek Toomre, PhD
Erdem Karatekin, PhD
Karin Reinisch, PhD
Thomas Melia, PhD
44Publications
4,278Citations
Legionella pneumophila
Macrophages
Coxiella burnetii
Publications
Featured Publications
Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System
Park D, Steiner S, Shao M, Roy CR, Liu J. Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System. Infection And Immunity 2022, 90: e00410-22. PMID: 36190257, PMCID: PMC9584302, DOI: 10.1128/iai.00410-22.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsSmall cell variantLarge cell variantDot/Icm type IV secretion systemCell variantIntracellular replicationActive large cell variantHost cellsIntracellular bacterial pathogenType IV secretion systemDot/Icm T4SSObligate intracellular bacterial pathogenC. burnetiiCoxiella burnetiiBiphasic developmental cycleUnique biphasic developmental cycleInfectionNew host cellsSecretion systemBacterial pathogensInfectious formHost vacuoleBurnetiiMorphological changesCellsLater stagesCover Image: Coxiella burnetii encodes an LvgA‐related protein important for intracellular replication (Cellular Microbiology 06/2021)
Steiner S, Meir A, Roy C. Cover Image: Coxiella burnetii encodes an LvgA‐related protein important for intracellular replication (Cellular Microbiology 06/2021). Cellular Microbiology 2021, 23 DOI: 10.1111/cmi.13351.Peer-Reviewed Original ResearchHost cell depletion of tryptophan by IFNγ-induced Indoleamine 2,3-dioxygenase 1 (IDO1) inhibits lysosomal replication of Coxiella burnetii
Ganesan S, Roy CR. Host cell depletion of tryptophan by IFNγ-induced Indoleamine 2,3-dioxygenase 1 (IDO1) inhibits lysosomal replication of Coxiella burnetii. PLOS Pathogens 2019, 15: e1007955. PMID: 31461509, PMCID: PMC6736304, DOI: 10.1371/journal.ppat.1007955.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsC. burnetiiC. burnetii replicationIntracellular replicationIntracellular pathogensPro-inflammatory cytokine interferon gammaIFNγ-induced genesCell-autonomous defense mechanismAbsence of IFNγCytokine interferon-gammaMost intracellular pathogensMacrophage-like cellsKynurenine metabolitesCell depletionEffector mechanismsPathogen Coxiella burnetiiInterferon gammaIFNγTryptophan availabilityHost defenseDioxygenase 1Coxiella burnetiiTHP1 cellsRestrict replicationBacterial replicationBurnetiiAnalysis of Dot/Icm Type IVB Secretion System Subassemblies by Cryoelectron Tomography Reveals Conformational Changes Induced by DotB Binding
Park D, Chetrit D, Hu B, Roy CR, Liu J. Analysis of Dot/Icm Type IVB Secretion System Subassemblies by Cryoelectron Tomography Reveals Conformational Changes Induced by DotB Binding. MBio 2020, 11: 10.1128/mbio.03328-19. PMID: 32071271, PMCID: PMC7029142, DOI: 10.1128/mbio.03328-19.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsType IV secretion systemSecretion systemCryoelectron tomographyInner membraneDot/Icm apparatusConformational changesDot/IcmEukaryotic host cellsBacterial inner membraneWild-type cellsHost cell membraneWhole-cell contextMultiprotein nanomachineSubtomogram analysisSophisticated nanomachinesCytoplasmic substratesProtein effectorsCell polesDNA substratesSubtomogram averagingATPase complexDNA transferHost infectionStructural basisHost cellsA unique cytoplasmic ATPase complex defines the Legionella pneumophila type IV secretion channel
Chetrit D, Hu B, Christie PJ, Roy CR, Liu J. A unique cytoplasmic ATPase complex defines the Legionella pneumophila type IV secretion channel. Nature Microbiology 2018, 3: 678-686. PMID: 29784975, PMCID: PMC5970066, DOI: 10.1038/s41564-018-0165-z.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsCytoplasmic complexType IV secretion channelType IV secretion systemInner membrane complexTranslocation of substratesCryo-electron tomographySecretion channelCell polesCytoplasmic ATPaseSecretion systemT4SS functionHexameric assemblyMembrane complexCytoplasmic channelsDNA complexesSubstrate transferT4SSChannel activationATPaseComplexesDistinct stagesAssemblyBiogenesisATPasesFurther analysisMultiple Legionella pneumophila effector virulence phenotypes revealed through high-throughput analysis of targeted mutant libraries
Shames SR, Liu L, Havey JC, Schofield WB, Goodman AL, Roy CR. Multiple Legionella pneumophila effector virulence phenotypes revealed through high-throughput analysis of targeted mutant libraries. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: e10446-e10454. PMID: 29133401, PMCID: PMC5715750, DOI: 10.1073/pnas.1708553114.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsMeSH KeywordsAnimalsBacterial ProteinsCytotoxicity, ImmunologicDisease Models, AnimalFemaleGene Expression Regulation, BacterialHigh-Throughput Nucleotide SequencingHost-Pathogen InteractionsHumansLegionella pneumophilaLegionnaires' DiseaseLoss of Function MutationMiceMice, Inbred C57BLMutagenesis, InsertionalPhenotypeVirulenceConceptsCultured host cellsHost cellsEffector proteinsHost immune systemFunction mutationsVirulence phenotypesSevere pneumoniaInfected miceBacterial clearanceEffector mutantsLarge cohortMouse modelDot/Icm type IV secretion systemImmune systemType IV secretion systemScreen resultsEffector protein activityDifferent effector proteinsGenetic complementation studiesLegionnaires' diseaseCausative agentHost pathogenesisDifferent virulence phenotypesInfectionDiseaseThe Legionella Anti-autophagy Effector RavZ Targets the Autophagosome via PI3P- and Curvature-Sensing Motifs
Horenkamp FA, Kauffman KJ, Kohler LJ, Sherwood RK, Krueger KP, Shteyn V, Roy CR, Melia TJ, Reinisch KM. The Legionella Anti-autophagy Effector RavZ Targets the Autophagosome via PI3P- and Curvature-Sensing Motifs. Developmental Cell 2015, 34: 569-576. PMID: 26343456, PMCID: PMC4594837, DOI: 10.1016/j.devcel.2015.08.010.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsATG8 proteinsIntracellular pathogen Legionella pneumophilaPre-autophagosomal structureAtg8/LC3 proteinsPathogen Legionella pneumophilaHigh-curvature membranesMembrane transport pathwaysCytosol of cellsEffector proteinsCatalytic domainHost cytosolRavZAutophagy proteinsLC3 proteinPathogenic microbesSubstrate affinityProteinIntermediate membraneLegionella pneumophilaAutophagosomesAutophagyCytosolTransport pathwaysInterfacial activationMembraneA Screen of Coxiella burnetii Mutants Reveals Important Roles for Dot/Icm Effectors and Host Autophagy in Vacuole Biogenesis
Newton HJ, Kohler LJ, McDonough JA, Temoche-Diaz M, Crabill E, Hartland EL, Roy CR. A Screen of Coxiella burnetii Mutants Reveals Important Roles for Dot/Icm Effectors and Host Autophagy in Vacuole Biogenesis. PLOS Pathogens 2014, 10: e1004286. PMID: 25080348, PMCID: PMC4117601, DOI: 10.1371/journal.ppat.1004286.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsHost autophagyTransposon insertionDot/Icm effectorsDot/Icm systemAutophagosome protein LC3Intracellular growth defectPathogen-occupied vacuolesTransposon insertion mutantsVacuole biogenesisEffector proteinsInsertion mutantsModification enzymesGrowth defectArrayed libraryCentral metabolismIcm systemMutantsMolecular mechanismsVisual screenProtein LC3Host cellsIntracellular replicationGenesIntracellular pathogensRegulatory systemThe Machinery at Endoplasmic Reticulum-Plasma Membrane Contact Sites Contributes to Spatial Regulation of Multiple Legionella Effector Proteins
Hubber A, Arasaki K, Nakatsu F, Hardiman C, Lambright D, De Camilli P, Nagai H, Roy CR. The Machinery at Endoplasmic Reticulum-Plasma Membrane Contact Sites Contributes to Spatial Regulation of Multiple Legionella Effector Proteins. PLOS Pathogens 2014, 10: e1004222. PMID: 24992562, PMCID: PMC4081824, DOI: 10.1371/journal.ppat.1004222.Peer-Reviewed Original ResearchCitationsAltmetricAMPylation Is Critical for Rab1 Localization to Vacuoles Containing Legionella pneumophila
Hardiman CA, Roy CR. AMPylation Is Critical for Rab1 Localization to Vacuoles Containing Legionella pneumophila. MBio 2014, 5: 10.1128/mbio.01035-13. PMID: 24520063, PMCID: PMC3950522, DOI: 10.1128/mbio.01035-13.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsConceptsLCV membraneEffector proteinsGEF activityEndoplasmic reticulumIntracellular pathogen Legionella pneumophilaLegionella effector proteinsType IV secretion systemExchange factor domainLegionella pneumophilaMembrane-bound compartmentsMembrane-bound organellesPathogen Legionella pneumophilaAccumulation of GTPHost cell functionsAMPylation activityDrrA proteinRab1 proteinRab1 recruitmentLegionella effectorsNucleotidyltransferase domainAMPylationSecretion systemPosttranslational modificationsRab1GTPase
News
News
- September 25, 2019
Roy Appointed Director of the Yale Combined Program in the Biological and Biomedical Sciences (BBS)
- October 27, 2016
ABRCMS 2016
- September 30, 2016
New Appointments
- April 21, 2016
Craig Roy designated the Von Zedtwitz Professor of Microbial Pathogenesis
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Email
Mailing Address
Microbial Pathogenesis
Department of Microbial Pathogenesis, 295 Congress Avenue, BCMM 354B
New Haven, CT 06536-0812
United States