Chenxiang Lin, PhD
Associate Professor of Cell Biology and of Biomedical EngineeringDownloadHi-Res Photo
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Appointments
Cell Biology
Primary
Additional Titles
Associate Director, Nanobiology Institute
Contact Info
Appointments
Cell Biology
Primary
Additional Titles
Associate Director, Nanobiology Institute
Contact Info
Appointments
Cell Biology
Primary
Additional Titles
Associate Director, Nanobiology Institute
Contact Info
About
Titles
Associate Professor of Cell Biology and of Biomedical Engineering
Associate Director, Nanobiology Institute
Appointments
Cell Biology
Associate Professor TenurePrimary
Other Departments & Organizations
Education & Training
- Research Fellow
- Harvard Medical School (2012)
- PhD
- Arizona State University (2009)
- BS
- Peking University (2004)
Research
Overview
Medical Subject Headings (MeSH)
Biophysics; Biosensing Techniques; Cell Biology; Cell Membrane; DNA; Lipid Bilayers; Membrane Fusion; Membranes, Artificial; Nanostructures; Nanotechnology; Nuclear Pore; Nucleic Acid Probes; Reference Standards
ORCID
0000-0001-7041-1946- View Lab Website
Chenxiang Lin Lab
Research at a Glance
Yale Co-Authors
Frequent collaborators of Chenxiang Lin's published research.
Publications Timeline
A big-picture view of Chenxiang Lin's research output by year.
Research Interests
Research topics Chenxiang Lin is interested in exploring.
Qiancheng Xiong
Longfei Liu
Chunxiang Wu
Erdem Karatekin, PhD
C. Patrick Lusk, PhD
Yong Xiong, PhD
57Publications
4,620Citations
DNA
Nanostructures
Nanotechnology
Lipid Bilayers
Cell Membrane
Biosensing Techniques
Publications
Featured Publications
Modeling HIV-1 nuclear entry with nucleoporin-gated DNA-origami channels
Shen Q, Feng Q, Wu C, Xiong Q, Tian T, Yuan S, Shi J, Bedwell G, Yang R, Aiken C, Engelman A, Lusk C, Lin C, Xiong Y. Modeling HIV-1 nuclear entry with nucleoporin-gated DNA-origami channels. Nature Structural & Molecular Biology 2023, 30: 425-435. PMID: 36807645, PMCID: PMC10121901, DOI: 10.1038/s41594-023-00925-9.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsNuclear pore complexHIV-1 nuclear entryNuclear entryNuclear importNPC central channelPore complexHost nucleusCapsid dockingVirus genomeAffinity gradientNup153Central channelMechanistic insightsMolecular interactionsCapsidNucleoporinsNup358Nup62GenomeNucleusVirusDockingVirus-1 infectionImportComplexesActuating tension-loaded DNA clamps drives membrane tubulation
Liu L, Xiong Q, Xie C, Pincet F, Lin C. Actuating tension-loaded DNA clamps drives membrane tubulation. Science Advances 2022, 8: eadd1830. PMID: 36223466, PMCID: PMC9555772, DOI: 10.1126/sciadv.add1830.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsDNA clampMembrane tubulationMembrane dynamicsMembrane-remodeling eventsVesicle tubulationConformational changesSpatiotemporal controlDNA signalsCell membraneDNA nanostructuresTubulationMembrane deformationClosed stateOpen stateSelf-assembled DNA nanostructuresOrganismsProteinMembrane tubeArtificial systemsTube widthMembraneDynamicsSorting sub-150-nm liposomes of distinct sizes by DNA-brick-assisted centrifugation
Yang Y, Wu Z, Wang L, Zhou K, Xia K, Xiong Q, Liu L, Zhang Z, Chapman ER, Xiong Y, Melia TJ, Karatekin E, Gu H, Lin C. Sorting sub-150-nm liposomes of distinct sizes by DNA-brick-assisted centrifugation. Nature Chemistry 2021, 13: 335-342. PMID: 33785892, PMCID: PMC8049973, DOI: 10.1038/s41557-021-00667-5.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsFunctionalized DNA-Origami-Protein Nanopores Generate Large Transmembrane Channels with Programmable Size-Selectivity
Shen Q, Xiong Q, Zhou K, Feng Q, Liu L, Tian T, Wu C, Xiong Y, Melia T, Lusk C, Lin C. Functionalized DNA-Origami-Protein Nanopores Generate Large Transmembrane Channels with Programmable Size-Selectivity. Journal Of The American Chemical Society 2022, 145: 1292-1300. PMID: 36577119, PMCID: PMC9852090, DOI: 10.1021/jacs.2c11226.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsExchange of macromoleculesCholesterol-rich membranesHybrid nanoporesSynthetic biologyBiophysical toolsSynthetic cellsTransmembrane channelsTransmembrane nanoporesDNA ringsProtein nanoporeCell membraneBacterial toxinsDNA origami techniqueLipid membranesAnalytical chemistryMacromolecule sizeDNA origamiMembraneProgrammable sizeNanoporesSized poresNucleoporinsAverage inner diameterCellsPneumolysinA programmable DNA-origami platform for studying lipid transfer between bilayers
Bian X, Zhang Z, Xiong Q, De Camilli P, Lin C. A programmable DNA-origami platform for studying lipid transfer between bilayers. Nature Chemical Biology 2019, 15: 830-837. PMID: 31320758, PMCID: PMC6650167, DOI: 10.1038/s41589-019-0325-3.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsLipid transferNon-vesicular lipid transportSynaptotagmin-like mitochondrial lipid-binding protein (SMP) domainLipid transportMembrane contact sitesLipid transport proteinsSMP domainImportant physiological roleDNA origami platformProtein domainsUnstructured linkerContact sitesSynaptotagmin-1Förster resonance energy transferPhysiological roleResonance energy transferMechanistic insightsDNA origami nanostructuresAcceptor liposomesVesicle Tubulation with Self‐Assembling DNA Nanosprings
Grome MW, Zhang Z, Pincet F, Lin C. Vesicle Tubulation with Self‐Assembling DNA Nanosprings. Angewandte Chemie International Edition 2018, 57: 5330-5334. PMID: 29575478, PMCID: PMC5924453, DOI: 10.1002/anie.201800141.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsMembrane-deforming proteinsDNA origami designMembrane tubulationMembrane tubulesMembrane curvatureMembrane surface coverageVesicle tubulationDNA structureLipid bilayersTubulationNanospringsTube morphologyIntricate interplayArtificial nanomachinesVesicle deformationSpherical vesiclesNanotechnologyMajor goalProteinDNAVesiclesNanomachinesBioengineeringDetergentsMorphologyA Programmable DNA Origami Platform for Organizing Intrinsically Disordered Nucleoporins within Nanopore Confinement
Fisher PDE, Shen Q, Akpinar B, Davis LK, Chung KKH, Baddeley D, Šarić A, Melia TJ, Hoogenboom BW, Lin C, Lusk CP. A Programmable DNA Origami Platform for Organizing Intrinsically Disordered Nucleoporins within Nanopore Confinement. ACS Nano 2018, 12: 1508-1518. PMID: 29350911, PMCID: PMC5834394, DOI: 10.1021/acsnano.7b08044.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsTransport channelsAtomic force microscopyMolecular dynamics simulationsHigh-speed atomic force microscopyDNA origami platformFG domainsNuclear pore complexes (NPCs) formChannel mimicsCentral transport channelNuclear pore proteinsForce microscopyDNA origamiNuclear transport receptorsDynamics simulationsSelective transportNanopore confinementMolecular exchangePermeability propertiesDNA cylindersChemical compositionFG networkPore proteinsPolymer modelTransport receptorsCollective propertiesPlacing and shaping liposomes with reconfigurable DNA nanocages
Zhang Z, Yang Y, Pincet F, Llaguno M, Lin C. Placing and shaping liposomes with reconfigurable DNA nanocages. Nature Chemistry 2017, 9: 653-659. PMID: 28644472, PMCID: PMC5542812, DOI: 10.1038/nchem.2802.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsMembrane-bound vesiclesDNA cagesRegulated deformationsDNA nanocagesMembrane curvatureMembrane fusionConformational changesBiological membranesCell membraneLipid bilayer membranesMembrane mechanicsVesiclesDiverse structuresMembraneCellsBilayer membranesVersatile toolDelivery vesiclesToroid shapeLiposome shapeSelf-assembly of size-controlled liposomes on DNA nanotemplates
Yang Y, Wang J, Shigematsu H, Xu W, Shih WM, Rothman JE, Lin C. Self-assembly of size-controlled liposomes on DNA nanotemplates. Nature Chemistry 2016, 8: 476-483. PMID: 27102682, PMCID: PMC5021307, DOI: 10.1038/nchem.2472.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsDNA nanotemplatesArtificial lipid bilayer membranesLipid bilayer formationLipid bilayer membranesSelf-AssemblyTemplating methodKey intermediateBilayer formationDrug deliveryArtificial vesiclesLipid compositionNanoscale precisionNanotemplatesHomogeneous liposomesUnilamellar vesiclesLiposome formationVesicular transportProtein structureMembrane structureVesicle sizeLiposomesIntermediatesFormationVesiclesStructurePurification of DNA-origami nanostructures by rate-zonal centrifugation
Lin C, Perrault SD, Kwak M, Graf F, Shih WM. Purification of DNA-origami nanostructures by rate-zonal centrifugation. Nucleic Acids Research 2012, 41: e40-e40. PMID: 23155067, PMCID: PMC3553994, DOI: 10.1093/nar/gks1070.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsDNA origami nanostructuresDNA nanostructuresDNA origami structuresAgarose gel electrophoresisDNA origami constructsNanostructuresAqueous solutionFraction collectionSeparation resolutionGradient mixingPurification processHigh yieldsPurification approachConcentration stepRate zonal centrifugationCentrifuge tubeAgarose gelDyeAvailable equipmentDesaltingGelContaminantsPurificationPreparationSeparation
Academic Achievements & Community Involvement
honor Odyssey Award
Regional AwardRichard and Susan Smith Family FoundationDetails09/01/2020United Stateshonor Director's New Innovator Award
National AwardNational Institutes of HealthDetails09/30/2014United States
News
News
- February 19, 2023Source: Nature Structural & Molecular Biology
Modeling HIV-1 nuclear entry with DNA-origami nuclear pore mimics
- May 02, 2022Source: Nature Methods
A better way to PAINT your sample for super-resolution imaging
- March 30, 2021Source: Nature Chemistry
DNA helps sort out liposomes and biophysical problems
- February 07, 2021Source: BioRxiv
A DNA-origami NanoTrap for studying the diffusion barriers
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Chenxiang Lin Lab
Lab
West Campus Integrative Science & Technology Center
850 West Campus Drive, Rm 213
West Haven, CT 06516