Venkat Kalyana Sundaram, PhD
Research Scientist in Cell BiologyCards
About
Research
Publications
Featured Publications
Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip
Sundaram R, Bera M, Coleman J, Weerakkody J, Krishnakumar S, Ramakrishnan S. Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip. Small 2022, 18: e2205567. PMID: 36328714, DOI: 10.1002/smll.202205567.Peer-Reviewed Original ResearchMunc13 structural transitions and oligomers that may choreograph successive stages in vesicle priming for neurotransmitter release
Grushin K, Kalyana Sundaram RV, Sindelar CV, Rothman JE. Munc13 structural transitions and oligomers that may choreograph successive stages in vesicle priming for neurotransmitter release. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2121259119. PMID: 35135883, PMCID: PMC8851502, DOI: 10.1073/pnas.2121259119.Peer-Reviewed Original ResearchMunc13 binds and recruits SNAP25 to chaperone SNARE complex assembly
Sundaram R, Jin H, Li F, Shu T, Coleman J, Yang J, Pincet F, Zhang Y, Rothman JE, Krishnakumar SS. Munc13 binds and recruits SNAP25 to chaperone SNARE complex assembly. FEBS Letters 2020, 595: 297-309. PMID: 33222163, PMCID: PMC8068094, DOI: 10.1002/1873-3468.14006.Peer-Reviewed Original ResearchAnimalsBinding SitesCloning, MolecularEscherichia coliGene ExpressionGenetic VectorsLipid BilayersLiposomesMiceModels, MolecularNerve Tissue ProteinsOptical TweezersPhosphatidylcholinesPhosphatidylethanolaminesPhosphatidylserinesPolyethylene GlycolsProtein BindingProtein Conformation, alpha-HelicalProtein Conformation, beta-StrandProtein Interaction Domains and MotifsRecombinant Fusion ProteinsSynaptosomal-Associated Protein 25Syntaxin 1Vesicle-Associated Membrane Protein 2Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery
Sundaram R, Chatterjee A, Bera M, Grushin K, Panda A, Li F, Coleman J, Lee S, Ramakrishnan S, Ernst A, Gupta K, Rothman J, Krishnakumar S. Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2309516120. PMID: 37590407, PMCID: PMC10450444, DOI: 10.1073/pnas.2309516120.Peer-Reviewed Original ResearchThis study investigates how Munc13 and Munc18 proteins cooperate to prime synaptic vesicles for rapid neurotransmitter release, revealing key roles of diacylglycerol in enhancing vesicle readiness and calcium-triggered fusion.
2026
Synaptic transmission: Munc13 assembles onto PI(4,5)P2-rich domains into trimers that cooperate to capture vesicles
Li F, Radhakrishnan A, Gautam S, Diaz G, Sundaram R, Coleman J, Zheng H, Grushin K, Chavent M, Rothman J, Pincet F. Synaptic transmission: Munc13 assembles onto PI(4,5)P2-rich domains into trimers that cooperate to capture vesicles. Proceedings Of The National Academy Of Sciences Of The United States Of America 2026, 123: e2523347123. PMID: 41671179, PMCID: PMC12912961, DOI: 10.1073/pnas.2523347123.Peer-Reviewed Original Research
2025
Two successive oligomeric Munc13 assemblies scaffold vesicle docking and SNARE assembly to support neurotransmitter release
Bera M, Grushin K, Kalyana Sundaram R, Hinzen J, Chen J, Chatterjee A, Radhakrishnan A, Lee S, Padmanarayana M, Coleman J, Pincet F, Rothman J, Dittman J. Two successive oligomeric Munc13 assemblies scaffold vesicle docking and SNARE assembly to support neurotransmitter release. Nature Communications 2025, 16: 7222. PMID: 40764329, PMCID: PMC12325603, DOI: 10.1038/s41467-025-62420-7.Peer-Reviewed Original ResearchHighly stable planar asymmetric suspended membranes for investigating protein dynamics and membrane fusion
Bera M, Kalyana Sundaram R, Coleman J, Chatterjee A, Thoduvayil S, Pincet F, Ramakrishnan S. Highly stable planar asymmetric suspended membranes for investigating protein dynamics and membrane fusion. Nature Protocols 2025, 20: 3582-3606. PMID: 40461798, DOI: 10.1038/s41596-025-01192-2.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2024
Quantitative single-molecule analysis of assembly and Ca2+-dependent disassembly of synaptotagmin oligomers on lipid bilayers
Li F, Coleman J, Redondo-Morata L, Kalyana Sundaram R, Stroeva E, Rothman J, Pincet F. Quantitative single-molecule analysis of assembly and Ca2+-dependent disassembly of synaptotagmin oligomers on lipid bilayers. Communications Biology 2024, 7: 1608. PMID: 39627539, PMCID: PMC11615320, DOI: 10.1038/s42003-024-07317-9.Peer-Reviewed Original Research
2023
Synaptophysin chaperones the assembly of 12 SNAREpins under each ready-release vesicle
Bera M, Radhakrishnan A, Coleman J, Sundaram R, Ramakrishnan S, Pincet F, Rothman J. Synaptophysin chaperones the assembly of 12 SNAREpins under each ready-release vesicle. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2311484120. PMID: 37903271, PMCID: PMC10636311, DOI: 10.1073/pnas.2311484120.Peer-Reviewed Original ResearchRapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin Therapy
Thoduvayil S, Weerakkody J, Sundaram R, Topper M, Bera M, Coleman J, Li X, Mariappan M, Ramakrishnan S. Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin Therapy. Cell Calcium 2023, 113: 102766. PMID: 37295201, PMCID: PMC10450995, DOI: 10.1016/j.ceca.2023.102766.Peer-Reviewed Original Research