Venkat Kalyana Sundaram, PhD
Research Scientist in Cell BiologyCards
About
Research
Publications
Featured Publications
Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip
Sundaram R, Bera M, Coleman J, Weerakkody J, Krishnakumar S, Ramakrishnan S. Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip. Small 2022, 18: e2205567. PMID: 36328714, DOI: 10.1002/smll.202205567.Peer-Reviewed Original ResearchConceptsPlasma membraneProtein assembliesNative plasma membrane vesiclesTotal internal reflection fluorescence microscopySingle-molecule levelSingle-molecule investigationsCellular plasma membranePlasma membrane vesiclesSingle-molecule precisionReflection fluorescence microscopyKnowledge of lipidProtein complexesProtein architectureCell signalingMovement of moleculesProtein orientationLipid membranesBiological processesCellular membranesMembrane vesiclesMicroarray platformFluorescence investigationsLipid domainsFluorescence microscopyMembraneMunc13 structural transitions and oligomers that may choreograph successive stages in vesicle priming for neurotransmitter release
Grushin K, Kalyana Sundaram RV, Sindelar CV, Rothman JE. Munc13 structural transitions and oligomers that may choreograph successive stages in vesicle priming for neurotransmitter release. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2121259119. PMID: 35135883, PMCID: PMC8851502, DOI: 10.1073/pnas.2121259119.Peer-Reviewed Original ResearchMunc13 binds and recruits SNAP25 to chaperone SNARE complex assembly
Sundaram R, Jin H, Li F, Shu T, Coleman J, Yang J, Pincet F, Zhang Y, Rothman JE, Krishnakumar SS. Munc13 binds and recruits SNAP25 to chaperone SNARE complex assembly. FEBS Letters 2020, 595: 297-309. PMID: 33222163, PMCID: PMC8068094, DOI: 10.1002/1873-3468.14006.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCloning, MolecularEscherichia coliGene ExpressionGenetic VectorsLipid BilayersLiposomesMiceModels, MolecularNerve Tissue ProteinsOptical TweezersPhosphatidylcholinesPhosphatidylethanolaminesPhosphatidylserinesPolyethylene GlycolsProtein BindingProtein Conformation, alpha-HelicalProtein Conformation, beta-StrandProtein Interaction Domains and MotifsRecombinant Fusion ProteinsSynaptosomal-Associated Protein 25Syntaxin 1Vesicle-Associated Membrane Protein 2ConceptsSNARE complex assemblyComplex assemblyMunc13-1 MUN domainDetailed structure-function analysisSNARE protein VAMP2Syntaxin 1/Structure-function analysisSynaptic vesicle fusionOptical tweezers studiesSNARE assemblySNARE motifMUN domainMunc18-1Syntaxin-1Munc13-1FRET spectroscopyLinker regionVesicle fusionDirect bindingPhospholipid bilayersPresynaptic membraneSNAP25AssemblyBindingRecruits
2023
Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin Therapy
Sikha Thoduvayil, Jonathan S. Weerakkody, Ramalingam Venkat Kalyana Sundaram, Mackenzie Topper, Manindra Bera, Jeff Coleman, Xia Li, Malaiyalam Mariappan, Sathish Ramakrishnan. Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin Therapy, Cell Calcium, Volume 113, 2023, 102766, ISSN 0143-4160, https://doi.org/10.1016/j.ceca.2023.102766. (https://www.sciencedirect.com/science/article/pii/S0143416023000787)Peer-Reviewed Original Research In Press
2021
Vesicle capture by membrane‐bound Munc13‐1 requires self‐assembly into discrete clusters
Li F, Sundaram R, Gatta AT, Coleman J, Ramakrishnan S, Krishnakumar SS, Pincet F, Rothman JE. Vesicle capture by membrane‐bound Munc13‐1 requires self‐assembly into discrete clusters. FEBS Letters 2021, 595: 2185-2196. PMID: 34227103, DOI: 10.1002/1873-3468.14157.Peer-Reviewed Original ResearchConceptsMunc13-1Vesicle captureSpecific plasma membrane domainsStep-wise photobleachingC-domainMunc13-1 proteinPlasma membrane domainsSynaptic vesicle dockingC-terminal CVesicle dockingMembrane domainsTIRF microscopySoluble proteinVesicle membraneActive zoneMultiple copiesSynaptic vesiclesFunctional significanceSmall unilamellar vesiclesLipid bilayersVesiclesUnilamellar vesiclesProteinDiscrete clustersCopies
2010
Multifunctional carbon-nanotube cellular endoscopes
Singhal R, Orynbayeva Z, Kalyana Sundaram RV, Niu JJ, Bhattacharyya S, Vitol EA, Schrlau MG, Papazoglou ES, Friedman G, Gogotsi Y. Multifunctional carbon-nanotube cellular endoscopes. Nature Nanotechnology 2010, 6: 57-64. PMID: 21151109, DOI: 10.1038/nnano.2010.241.Peer-Reviewed Original ResearchConceptsAtomic force microscope tipForce microscope tipAttolitre volumesMagnetic nanoparticlesIntracellular probingSingle cell surgeryCarbon nanotubesDrug deliveryFluid handlingConventional glass micropipettesMicroscope tipElectrochemical diagnosticsNanoparticlesNanotubesSingle organelle levelConical geometryNanoneedlesIntracellular environmentSpatial resolutionOrganelle levelGlass micropipettesGlass pipetteBroad rangeDevicesDiagnostics