Jonathan Weerakkody, PhD
Postdcoctoral AssociateAbout
Research
Publications
2024
CNS cell-derived exosome signatures as blood-based biomarkers of neurodegenerative diseases
Park, C., Weerakkody, J. S., Schneider, R., Miao, S., & Pitt, D. (2024). CNS cell-derived exosome signatures as blood-based biomarkers of neurodegenerative diseases. Frontiers in Neuroscience, 18, 1426700.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsCNS cell-derived exosome signatures as blood-based biomarkers of neurodegenerative diseases
Park C, Weerakkody J, Schneider R, Miao S, Pitt D. CNS cell-derived exosome signatures as blood-based biomarkers of neurodegenerative diseases. Frontiers In Neuroscience 2024, 18: 1426700. PMID: 38966760, PMCID: PMC11222337, DOI: 10.3389/fnins.2024.1426700.Peer-Reviewed Original ResearchNeurodegenerative diseasesSubtype of extracellular vesiclesDisease-related processesDisease-associated changesCentral nervous systemBlood-based proteinsBiomarkers of neurodegenerative diseasesBlood-brain barrierCell typesExosome populationMolecular cargoExtracellular vesiclesCell of originRNA biomarkersExosome isolationTranslation to clinical useCell-derived exosomesLack of standardized methodologyMolecular contentExosomesBlood-based biomarkersPeripheral bloodCellsExosome signatureMolecular biomarkersPhotosensitive Nanoprobes for Rapid High Purity Isolation and Size-Specific Enrichment of Synthetic and Extracellular Vesicle Subpopulations
Weerakkody, Jonathan S., Tiffany Tseng, Mackenzie Topper, Sikha Thoduvayil, Abhijith Radhakrishnan, Frederic Pincet, Themis R. Kyriakides, Roshan W. Gunasekara, and Sathish Ramakrishnan. "Photosensitive Nanoprobes for Rapid High Purity Isolation and Size‐Specific Enrichment of Synthetic and Extracellular Vesicle Subpopulations." Advanced Functional Materials (2024): 2400390.Peer-Reviewed Original Research
2023
Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin Therapy
Thoduvayil S, Weerakkody J, Sundaram R, Topper M, Bera M, Coleman J, Li X, Mariappan M, Ramakrishnan S. Rapid Quantification of First and Second Phase Insulin Secretion Dynamics using an In vitro Platform for Improving Insulin Therapy. Cell Calcium 2023, 113: 102766. PMID: 37295201, PMCID: PMC10450995, DOI: 10.1016/j.ceca.2023.102766.Peer-Reviewed Original ResearchConceptsCellular pathwaysSecretion dynamicsSmall molecule screeningLuciferase reporter systemInsulin secretion dynamicsHigh-throughput compoundInsulin secretionHigh-throughput quantificationReporter systemGenetic studiesLive cellsDistinct rolesInsulin therapyGlucose-stimulated insulin secretionSmall moleculesEffective insulin therapyPathwaySecretion
2022
Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip
Sundaram R, Bera M, Coleman J, Weerakkody J, Krishnakumar S, Ramakrishnan S. Native Planar Asymmetric Suspended Membrane for Single‐Molecule Investigations: Plasma Membrane on a Chip. Small 2022, 18: e2205567. PMID: 36328714, DOI: 10.1002/smll.202205567.Peer-Reviewed Original ResearchConceptsPlasma membraneProtein assembliesNative plasma membrane vesiclesTotal internal reflection fluorescence microscopySingle-molecule levelSingle-molecule investigationsCellular plasma membranePlasma membrane vesiclesSingle-molecule precisionReflection fluorescence microscopyKnowledge of lipidProtein complexesProtein architectureCell signalingMovement of moleculesProtein orientationLipid membranesBiological processesCellular membranesMembrane vesiclesMicroarray platformFluorescence investigationsLipid domainsFluorescence microscopyMembraneSurfactant-like Peptide Self-Assembled into Hybrid Nanostructures for Electronic Nose Applications.
Weerakkody JS, El Kazzy M, Jacquier E, Elchinger PH, Mathey R, Ling WL, Herrier C, Livache T, Buhot A, Hou Y. Surfactant-like Peptide Self-Assembled into Hybrid Nanostructures for Electronic Nose Applications. ACS Nano 2022, 16: 4444-4457. PMID: 35174710, DOI: 10.1021/acsnano.1c10734.Peer-Reviewed Original ResearchSuspended Lipid Bilayer: A Versatile Platform for Nextgen Drug Discovery and Biomedical Applications
Ramakrishnan, S. and Weerakkody, J.S., 2022. Suspended Lipid Bilayer: A Versatile Platform for Nextgen Drug Discovery and Biomedical Applications. Accounts of Materials Research, 3(10), pp.996-998.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2021
An Overview of Artificial Olfaction Systems with a Focus on Surface Plasmon Resonance for the Analysis of Volatile Organic Compounds.
El Kazzy M, Weerakkody JS, Hurot C, Mathey R, Buhot A, Scaramozzino N, Hou Y. An Overview of Artificial Olfaction Systems with a Focus on Surface Plasmon Resonance for the Analysis of Volatile Organic Compounds. Biosensors (Basel) 2021, 11 PMID: 34436046, DOI: 10.3390/bios11080244.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2020
Improvement of sensitivity of surface plasmon resonance imaging for the gas-phase detection of volatile organic compounds.
Brenet S, Weerakkody JS, Buhot A, Gallat FX, Mathey R, Leroy L, Livache T, Herrier C, Hou Y. Improvement of sensitivity of surface plasmon resonance imaging for the gas-phase detection of volatile organic compounds. Talanta 2020, 212: 120777. PMID: 32113542, DOI: 10.1016/j.talanta.2020.120777.Peer-Reviewed Original ResearchOptical Index Prism Sensitivity of Surface Plasmon Resonance Imaging in Gas Phase: Experiment versus Theory
Weerakkody, Jonathan S., Sophie Brenet, Thierry Livache, Cyril Herrier, Yanxia Hou, and Arnaud Buhot. "Optical index prism sensitivity of surface plasmon resonance imaging in gas phase: Experiment versus theory." The Journal of Physical Chemistry C 124, no. 6 (2020): 3756-3767.Peer-Reviewed Original Research