Ailong Ke
Eugene Higgins Professor of Molecular Biophysics and BiochemistryCards
About
Research
Publications
2026
Type IV-C CRISPR-Cas effector complexes recognize double-stranded DNA and switch on collateral cleavage of ssDNA and RNA
Pittman C, Xu C, Catchpole R, Garrett S, Fuchs R, Chu X, Makarova K, Koonin E, Zhao P, Wells L, Graveley B, Ke A, Terns M. Type IV-C CRISPR-Cas effector complexes recognize double-stranded DNA and switch on collateral cleavage of ssDNA and RNA. Cell Reports 2026, 45: 117939. PMID: 42700392, DOI: 10.1016/j.celrep.2026.117939.Peer-Reviewed Original ResearchStructure basis for single-strand nucleic acid targeting by IscB and variants
Xu C, Yang Q, Niu X, Ke A. Structure basis for single-strand nucleic acid targeting by IscB and variants. Nucleic Acids Research 2026, 54: gkag607. PMID: 42328786, PMCID: PMC13284715, DOI: 10.1093/nar/gkag607.Peer-Reviewed Original Research
2025
Conversion of IscB and Cas9 into RNA-guided RNA editors
Xu C, Niu X, Sun H, Yan H, Tang W, Ke A. Conversion of IscB and Cas9 into RNA-guided RNA editors. Cell 2025, 188: 5847-5861.e11. PMID: 40829585, PMCID: PMC12798835, DOI: 10.1016/j.cell.2025.07.032.Peer-Reviewed Original ResearchEscherichia coli yybP-ykoY Riboswitch as a Tandem Riboswitch Regulated by Mn2+ and pH
Xiao W, Liu G, Chen T, Zhang Y, Ke A, Cai R, Lu C. Escherichia coli yybP-ykoY Riboswitch as a Tandem Riboswitch Regulated by Mn2+ and pH. ACS Chemical Biology 2025, 20: 1010-1019. PMID: 40252020, PMCID: PMC12951517, DOI: 10.1021/acschembio.4c00715.Peer-Reviewed Original Research
2024
Assessing and engineering the IscB–ωRNA system for programmed genome editing
Yan H, Tan X, Zou S, Sun Y, Ke A, Tang W. Assessing and engineering the IscB–ωRNA system for programmed genome editing. Nature Chemical Biology 2024, 20: 1617-1628. PMID: 38977787, PMCID: PMC12213078, DOI: 10.1038/s41589-024-01669-3.Peer-Reviewed Original ResearchExploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications
Hu C, Myers M, Zhou X, Hou Z, Lozen M, Nam K, Zhang Y, Ke A. Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications. Molecular Cell 2024, 84: 463-475.e5. PMID: 38242128, PMCID: PMC10857747, DOI: 10.1016/j.molcel.2023.12.034.Peer-Reviewed Original Research
2023
Multiple adaptations underly co-option of a CRISPR surveillance complex for RNA-guided DNA transposition
Park J, Petassi M, Hsieh S, Mehrotra E, Schuler G, Budhathoki J, Truong V, Thyme S, Ke A, Kellogg E, Peters J. Multiple adaptations underly co-option of a CRISPR surveillance complex for RNA-guided DNA transposition. Molecular Cell 2023, 83: 1827-1838.e6. PMID: 37267904, PMCID: PMC10693918, DOI: 10.1016/j.molcel.2023.05.005.Peer-Reviewed Original Research
2022
Craspase is a CRISPR RNA-guided, RNA-activated protease
Hu C, van Beljouw S, Nam K, Schuler G, Ding F, Cui Y, Rodríguez-Molina A, Haagsma A, Valk M, Pabst M, Brouns S, Ke A. Craspase is a CRISPR RNA-guided, RNA-activated protease. Science 2022, 377: 1278-1285. PMID: 36007061, PMCID: PMC10041820, DOI: 10.1126/science.add5064.Peer-Reviewed Original ResearchAllosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools
Hu C, Ni D, Nam K, Majumdar S, McLean J, Stahlberg H, Terns M, Ke A. Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools. Molecular Cell 2022, 82: 2754-2768.e5. PMID: 35835111, PMCID: PMC9357151, DOI: 10.1016/j.molcel.2022.06.007.Peer-Reviewed Original ResearchStructural basis for RNA-guided DNA cleavage by IscB-ωRNA and mechanistic comparison with Cas9
Schuler G, Hu C, Ke A. Structural basis for RNA-guided DNA cleavage by IscB-ωRNA and mechanistic comparison with Cas9. Science 2022, 376: 1476-1481. PMID: 35617371, PMCID: PMC10041819, DOI: 10.1126/science.abq7220.Peer-Reviewed Original Research
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