Method for Calculation of Reversal Potential During the Persistent Activity
We calculated the average reversal potential of each time point (0.5 msec resolution) of the UP state by first averaging the individual traces of approximately 10 trials. Current versus voltage plots were then contructed for the DOWN state (blue lines) and different time points of the UP state (Red lines). The crossing point of the I-Vs was taken as the reversal potential of the UP state and the change in slope of the I-V plots indicated the change in conductance. The reversal potential and conductance were calculated throughout the UP state.