John Rose, PhD
Professor Emeritus of Pathology and Senior Research ScientistCards
Appointments
Additional Titles
Director, Molecular Virology Program
Contact Info
Appointments
Additional Titles
Director, Molecular Virology Program
Contact Info
Appointments
Additional Titles
Director, Molecular Virology Program
Contact Info
About
Titles
Professor Emeritus of Pathology and Senior Research Scientist
Director, Molecular Virology Program
Biography
Dr. Rose earned his Ph.D. at Stanford University in 1973 in the laboratory of Dr. Charles Yanofsky. His thesis research focused on regulation of the tryptophan operon of E. coli. He then did postdoctoral research at MIT in the laboratories of Drs. David Baltimore and Harvey Lodish, where he began work on eucaryotic RNA viruses. In 1978, Dr. Rose took a faculty position at the Salk Institute, where he continued work on RNA virus transcription, as well as structure, function, and transport of the vesicular stomatitis virus (VSV) glycoprotein. In 1986, he moved to become Professor of Pathology and Cell Biology at Yale University School of Medicine. In 1994, his laboratory developed a system for recovering non-segmented, negative-strand RNA viruses from DNA plasmids. His work at Yale during the past fifteen years has focused largely on new approaches to vaccine development using vectors based on recombinant VSV and other viral replicons. This work has led to development of robust vaccine platforms that can protect animals against numerous viral and bacterial pathogens, typically after a single dose. A VSV-based HIV vaccine advanced from the Rose laboratory has recently completed a successful Phase I clinical trial.
Appointments
Other Departments & Organizations
Education & Training
- Fellow
- Massachusetts Institute of Technology (1978)
- PhD
- Stanford University (1973)
- BS
- Brandeis University (1969)
Research
Overview
Medical Subject Headings (MeSH)
- View Lab Website
Rose Lab
Research at a Glance
Yale Co-Authors
Publications Timeline
Research Interests
David F. Stern, PhD
Publications
2014
RHABDOVIRUSES AS VACCINE VECTORS: FROM INITIAL DEVELOPMENT TO CLINICAL TRIALS
Rose J, Clarke D. RHABDOVIRUSES AS VACCINE VECTORS: FROM INITIAL DEVELOPMENT TO CLINICAL TRIALS. 2014, 199-230. DOI: 10.1142/9789814635349_0009.Peer-Reviewed Original ResearchCitations
2008
VSV/MVA vaccine rapidly elicits SIV antibodies and local and systemic SIV T cell responses in macaque neonates but does not prevent SIV dissemination after oral challenge
Abel K, Van Rompay K, Eastlick J, Moore J, Lemieux M, Schmidt K, Earl P, Buonocore-Buzzelli L, Moss B, Rose N, Rose J, McChesney M, Kozlowski P, Marthas M. VSV/MVA vaccine rapidly elicits SIV antibodies and local and systemic SIV T cell responses in macaque neonates but does not prevent SIV dissemination after oral challenge. Retrovirology 2008, 5: o15. DOI: 10.1186/1742-4690-5-s1-o15.Peer-Reviewed Original Research
2003
Intranasal Vaccination with a Recombinant Vesicular Stomatitis Virus Expressing Cottontail Rabbit Papillomavirus L1 Protein Provides Complete Protection against Papillomavirus-Induced Disease
Reuter J, Vivas-Gonzalez B, Gomez D, Wilson J, Brandsma J, Greenstone H, Rose J, Roberts A. Intranasal Vaccination with a Recombinant Vesicular Stomatitis Virus Expressing Cottontail Rabbit Papillomavirus L1 Protein Provides Complete Protection against Papillomavirus-Induced Disease. Journal Of Virology 2003, 77: 2799-2799. PMCID: PMC141127, DOI: 10.1128/jvi.77.4.2799.2003.Peer-Reviewed Original Research
2001
Replication-Competent or Attenuated, Nonpropagating Vesicular Stomatitis Viruses Expressing Respiratory Syncytial Virus (RSV) Antigens Protect Mice against RSV Challenge
Kahn J, Roberts A, Weibel C, Buonocore L, Rose J. Replication-Competent or Attenuated, Nonpropagating Vesicular Stomatitis Viruses Expressing Respiratory Syncytial Virus (RSV) Antigens Protect Mice against RSV Challenge. Journal Of Virology 2001, 75: 11079-11087. PMID: 11602747, PMCID: PMC114687, DOI: 10.1128/jvi.75.22.11079-11087.2001.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsMeSH KeywordsAdministration, IntranasalAnimalsAntigens, ViralBase SequenceCell LineCricetinaeFemaleMiceMice, Inbred BALB CMolecular Sequence DataRespiratory Syncytial Virus InfectionsVaccines, AttenuatedVaccines, SyntheticVesicular stomatitis Indiana virusViral ProteinsViral VaccinesVirus ReplicationConceptsRespiratory syncytial virusRSV challengeRecombinant vesicular stomatitis virusVesicular stomatitis virusRSV FRespiratory syncytial virus GRSV-specific antibodiesDetectable neutralizing antibodiesDetectable serum antibodiesStomatitis virusIntranasal vaccinationRSV vaccineRSV GProtective immunitySyncytial virusDetectable serumNeutralizing antibodiesSerum antibodiesMouse modelRSV proteinsComplete protectionReplication competentAntibodiesG glycoproteinVirusOptimization of cottontail rabbit papilloma virus challenge technique
Reuter J, Gomez D, Brandsma J, Rose J, Roberts A. Optimization of cottontail rabbit papilloma virus challenge technique. Journal Of Virological Methods 2001, 98: 127-134. PMID: 11576639, DOI: 10.1016/s0166-0934(01)00370-6.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsHuman papilloma virusIntradermal injectionTattoo needlePapilloma volumeSquamous papillomaPapilloma incidenceAdvanced tumorsSpontaneous regressionPapilloma virusInvasive carcinomaPinpoint fociLarge papillomasPapillomasSterile bladePapilloma formationChallenge techniqueChallenge methodRabbit skinWeeksRabbitsDiseaseDomestic rabbitsVirusCRPVInjectionMechanisms of Loss of Foreign Gene Expression in Recombinant Vesicular Stomatitis Viruses
QuiƱones-Kochs M, Schnell M, Buonocore L, Rose J. Mechanisms of Loss of Foreign Gene Expression in Recombinant Vesicular Stomatitis Viruses. Virology 2001, 287: 427-435. PMID: 11531419, DOI: 10.1006/viro.2001.1058.Peer-Reviewed Original ResearchCitationsAltmetricAn Effective AIDS Vaccine Based on Live Attenuated Vesicular Stomatitis Virus Recombinants
Rose N, Marx P, Luckay A, Nixon D, Moretto W, Donahoe S, Montefiori D, Roberts A, Buonocore L, Rose J. An Effective AIDS Vaccine Based on Live Attenuated Vesicular Stomatitis Virus Recombinants. Cell 2001, 106: 539-549. PMID: 11551502, DOI: 10.1016/s0092-8674(01)00482-2.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsMeSH KeywordsAcquired Immunodeficiency SyndromeAIDS VaccinesAnimalsCD4 Lymphocyte CountCD4-Positive T-LymphocytesEnzyme-Linked Immunosorbent AssayGene Products, envGene Products, gagHIVHIV AntibodiesHumansImmunization, SecondaryMacaca mulattaMiceNeutralization TestsPilot ProjectsRecombinant Fusion ProteinsSAIDS VaccinesSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusT-Lymphocytes, CytotoxicTime FactorsVaccines, AttenuatedVaccines, SyntheticVesicular stomatitis Indiana virusViral LoadVirus SheddingVesicular Stomatitis Virus Glycoprotein Containing the Entire Green Fluorescent Protein on Its Cytoplasmic Domain Is Incorporated Efficiently into Virus Particles
Dalton K, Rose J. Vesicular Stomatitis Virus Glycoprotein Containing the Entire Green Fluorescent Protein on Its Cytoplasmic Domain Is Incorporated Efficiently into Virus Particles. Virology 2001, 279: 414-421. PMID: 11162797, DOI: 10.1006/viro.2000.0736.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsLarge cytoplasmic domainCytoplasmic domainVSV G proteinVesicular stomatitis virusGreen fluorescent proteinG proteinsGFP proteinWild-type G proteinFluorescent proteinShort cytoplasmic domainVesicular stomatitis virus glycoproteinStrong selectionVirus particlesExtra genesHeterotrimeric proteinGFP geneProtein sequencesWild-type virusFluorescent virus particlesStop codonVirus assemblyInfectious cloneGenesViral membraneAmino acids
2000
Glycoprotein Exchange Vectors Based on Vesicular Stomatitis Virus Allow Effective Boosting and Generation of Neutralizing Antibodies to a Primary Isolate of Human Immunodeficiency Virus Type 1
Rose N, Roberts A, Buonocore L, Rose J. Glycoprotein Exchange Vectors Based on Vesicular Stomatitis Virus Allow Effective Boosting and Generation of Neutralizing Antibodies to a Primary Isolate of Human Immunodeficiency Virus Type 1. Journal Of Virology 2000, 74: 10903-10910. PMID: 11069984, PMCID: PMC113169, DOI: 10.1128/jvi.74.23.10903-10910.2000.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsRecombinant VSV vectorVesicular stomatitis virusNeutralizing antibodiesPrimary isolatesVSV vectorsHigh-titer neutralizing antibodiesHuman immunodeficiency virus type 1Human immunodeficiency virus envelope proteinImmunodeficiency virus type 1HIV primary isolatesCross-neutralizing antibodiesRecombinant vesicular stomatitis virusVirus type 1Effective vaccine vectorAntibody titersWild-type vesicular stomatitis virusHIV envelopeForeign antigensImmune responseVirus envelope proteinVSV Indiana serotypeVaccine vectorType 1AntibodiesG protein genePresence of bovine viral diarrhea virus (BVDV) E2 glycoprotein in VSV recombinant particles and induction of neutralizing BVDV antibodies in mice
Grigera P, Marzocca M, Capozzo A, Buonocore L, Donis R, Rose J. Presence of bovine viral diarrhea virus (BVDV) E2 glycoprotein in VSV recombinant particles and induction of neutralizing BVDV antibodies in mice. Virus Research 2000, 69: 3-15. PMID: 10989181, DOI: 10.1016/s0168-1702(00)00164-7.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, ViralBase SequenceCattleCell LineChimeraCricetinaeDiarrhea Viruses, Bovine ViralDNA, RecombinantFemaleMembrane GlycoproteinsMiceMice, Inbred BALB CNeutralization TestsRecombinant ProteinsVaccines, SyntheticVesicular stomatitis Indiana virusViral Envelope ProteinsViral VaccinesConceptsBovine viral diarrhea virus (BVDV) E2BALB/c miceRecombinant vesicular stomatitis virusViral-induced diseasesC miceE2 antibodiesBVDV antibodiesVesicular stomatitis virusHigh avidityBVDV E2Recombinant E2Infected cellsDays post inoculationG glycoproteinE2Stomatitis virusMiceAntibodiesImmunofluorescence microscopyPost inoculationRecombinant particlesBHK21 cellsCellsSymptomsInfection
Academic Achievements & Community Involvement
honor National Institutes of Health MERIT award, 2003-2012
National AwardNIHDetailsUnited Stateshonor National Institutes of Health MERIT award, 1986-1996
National AwardNIHDetailsUnited States
News & Links
News
- April 29, 2020Source: YaleNews
Researchers at Yale Pursuing COVID-19 Vaccine Based on Powerful Yale Platform
- April 17, 2020
Laying the groundwork
- December 01, 2008
Grants and contracts awarded to Yale School of Medicine
- April 15, 2006
The virus behind the cancer
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203.785.6127Appointments
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