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Associate Research Scientist
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2018
Function and maturation of the Fe–S center in dihydroxyacid dehydratase from Arabidopsis
Gao H, Azam T, Randeniya S, Couturier J, Rouhier N, Johnson M. Function and maturation of the Fe–S center in dihydroxyacid dehydratase from Arabidopsis. Journal Of Biological Chemistry 2018, 293: 4422-4433. PMID: 29425096, PMCID: PMC5868250, DOI: 10.1074/jbc.ra117.001592.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsConceptsDihydroxyacid dehydrataseBranched-chain amino acid biosynthesisArabidopsis thaliana</i>Amino acid biosynthesisCombination of UV-visible absorptionFe-S clustersMechanism of catalysisCluster biogenesisAcid biosynthesisCluster donorProductive bindingCharacterized examplesReconstitution reactionArabidopsisOxygen-labileDihydroxyacidBiogenesisDehydrataseEnzymeFe centerCluster transferCatalytic rolePlantsNFU2Resonance Raman and electron paramagnetic resonance
2013
Arabidopsis thaliana Nfu2 Accommodates [2Fe-2S] or [4Fe-4S] Clusters and Is Competent for in Vitro Maturation of Chloroplast [2Fe-2S] and [4Fe-4S] Cluster-Containing Proteins
Gao H, Subramanian S, Couturier J, Naik S, Kim S, Leustek T, Knaff D, Wu H, Vignols F, Huynh B, Rouhier N, Johnson M. Arabidopsis thaliana Nfu2 Accommodates [2Fe-2S] or [4Fe-4S] Clusters and Is Competent for in Vitro Maturation of Chloroplast [2Fe-2S] and [4Fe-4S] Cluster-Containing Proteins. Biochemistry 2013, 52: 6633-6645. PMID: 24032747, PMCID: PMC3819817, DOI: 10.1021/bi4007622.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsYeast two-hybrid studiesIron-sulfur (Fe-SAdenosine 5'-phosphosulfate reductaseChloroplastic Fe-S proteinsReductive sulfur assimilationTwo-hybrid studiesBiosynthesis of cysteineFe-S cluster incorporationCombination of UV-visible absorptionNfu proteinsArabidopsis thalianaNFU2CXXC motifSulfur assimilationPlant metabolismFe proteinCluster transferGel filtration studiesArabidopsisFe-S clustersProteinGlutaredoxinUV-visible absorptionCysteineCluster incorporation
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