Felipe Passalia, PhD
Postdoctoral AssociateAbout
Research
Publications
2023
Lysinoalanine cross-linking is a conserved post-translational modification in the spirochete flagellar hook
Lynch M, Deshpande M, Kurniyati K, Zhang K, James M, Miller M, Zhang S, Passalia F, Wunder E, Charon N, Li C, Crane B. Lysinoalanine cross-linking is a conserved post-translational modification in the spirochete flagellar hook. PNAS Nexus 2023, 2: pgad349. PMID: 38047041, PMCID: PMC10691653, DOI: 10.1093/pnasnexus/pgad349.Peer-Reviewed Original ResearchMPL36, a major plasminogen (PLG) receptor in pathogenic Leptospira, has an essential role during infection
Zhu W, Passalia F, Hamond C, Abe C, Ko A, Barbosa A, Wunder E. MPL36, a major plasminogen (PLG) receptor in pathogenic Leptospira, has an essential role during infection. PLOS Pathogens 2023, 19: e1011313. PMID: 37486929, PMCID: PMC10399853, DOI: 10.1371/journal.ppat.1011313.Peer-Reviewed Original ResearchConceptsOuter membrane proteinsBacterial outer membrane proteinsC-terminal regionSurface-exposed regionsLeptospiral pathogenesisMDCK cell monolayersMembrane proteinsPathogenic LeptospiraImportant virulence factorMutant strainRecombinant proteinsPathogen's abilityVirulence mechanismsLysine residuesKoch's molecular postulatesBinding proteinPlasminogen receptorPathogen disseminationPathogenic spirochetesVirulence factorsProteinBacterial pathogensEssential roleMutantsHost tissuesCaracterização bioquímica e imunológica de proteínas hipotéticas de superfície de Leptospira interrogans
Passalia F. Caracterização bioquímica e imunológica de proteínas hipotéticas de superfície de Leptospira interrogans. 2023 DOI: 10.11606/t.87.2023.tde-13052024-110713.Peer-Reviewed Original Research
2021
A Review on Host-Leptospira Interactions: What We Know and Future Expectations
Daroz B, Fernandes L, Cavenague M, Kochi L, Passalia F, Takahashi M, Filho E, Teixeira A, Nascimento A. A Review on Host-Leptospira Interactions: What We Know and Future Expectations. Frontiers In Cellular And Infection Microbiology 2021, 11: 777709. PMID: 34900757, PMCID: PMC8657130, DOI: 10.3389/fcimb.2021.777709.Peer-Reviewed Original ResearchConceptsExtracellular matrixComplement system regulatorsHost-pathogen interactionsHost receptor moleculesBinding to componentsHost fibrinolytic systemMutagenesis toolCoagulation cascade componentsLeptospiral surfaceAdherence factorsGenetic manipulationInvasion mechanismsFunctional validationHost componentsComponents lamininCascade componentsProteinBacterial colonizationCell receptorsSurface plasminReceptor moleculesHostVeterinary concernComplement systemSystem regulationA Novel Leptospira interrogans Protein LIC13086 Inhibits Fibrin Clot Formation and Interacts With Host Components
Passalia F, Heinemann M, Vieira M, Nascimento A. A Novel Leptospira interrogans Protein LIC13086 Inhibits Fibrin Clot Formation and Interacts With Host Components. Frontiers In Cellular And Infection Microbiology 2021, 11: 708739. PMID: 34277477, PMCID: PMC8280789, DOI: 10.3389/fcimb.2021.708739.Peer-Reviewed Original ResearchConceptsMolecular mechanismsExtracellular matrix components lamininPathogenic spirochete bacteriaOuter membrane proteinsLeptospiral pathogenesisLeptospiral proteinsHost invasionMembrane proteinsInfection processHost componentsSpirochete bacteriaHost immune responseComponents lamininImmune evasion processProteinMultifunctional roleAdhesion processPlasma fibronectinFibrin clot formationHostClot formationEvasion mechanismsComplement systemInhibitory effectImmune response
2020
The Leptospira interrogans LIC10774 is a multifunctional surface protein that binds calcium and interacts with host components
Passalia F, Carvalho E, Heinemann M, Vieira M, Nascimento A. The Leptospira interrogans LIC10774 is a multifunctional surface protein that binds calcium and interacts with host components. Microbiological Research 2020, 235: 126470. PMID: 32247916, DOI: 10.1016/j.micres.2020.126470.Peer-Reviewed Original ResearchConceptsImmunofluorescence-based confocal microscopyAdhesion to host tissuesMolecular mechanismsHost extracellular matrixMultifunctional surface proteinLeptospiral outer membraneOuter membrane proteinsOuter membraneRecombinant proteinsMembrane proteinsPathogenic bacteriaBioinformatics analysisGenus LeptospiraTertiary structureSurface proteinsPathogenic leptospiresCalcium-binding proteinHost componentsCircular dichroism spectroscopyHydrophobic dye bindingFunctional roleProteinMultifunctional roleCalcium bindingExtracellular matrixLeptospira interrogans Bat proteins impair host hemostasis by fibrinogen cleavage and platelet aggregation inhibition
Passalia F, Heinemann M, de Andrade S, Nascimento A, Vieira M. Leptospira interrogans Bat proteins impair host hemostasis by fibrinogen cleavage and platelet aggregation inhibition. Medical Microbiology And Immunology 2020, 209: 201-213. PMID: 32078713, DOI: 10.1007/s00430-020-00664-4.Peer-Reviewed Original ResearchConceptsBat proteinsVWF-A domainEscherichia coliHost hemostasisA domainEnzymatic activityPathogenic LeptospiraSerine proteasesBinding sitesChromogenic substrateProteinSerovar CopenhageniPlatelet aggregationSpread zoonosisFibrinogen cleavagePlatelet aggregation inhibitionGenomeHemostasis dysfunctionAggregation inhibitionVWF/ristocetinGenesHemostatic assaysPathophysiological mechanismsSerineHemostatic impairment
2019
Adjuvanted leptospiral vaccines: Challenges and future development of new leptospirosis vaccines
Teixeira A, Fernandes L, Cavenague M, Takahashi M, Santos J, Passalia F, Daroz B, Kochi L, Vieira M, Nascimento A. Adjuvanted leptospiral vaccines: Challenges and future development of new leptospirosis vaccines. Vaccine 2019, 37: 3961-3973. PMID: 31186193, DOI: 10.1016/j.vaccine.2019.05.087.Peer-Reviewed Original ResearchConceptsDisease of global importanceAntigens to antigen-presenting cellsImmune responseControl of leptospirosisPrevention of leptospirosisBacteria-derived componentsEffective vaccineAntigen-presenting cellsRecombinant protein-based vaccinesLeptospiral vaccinesLeptospirosis vaccineBacterinDevelopment of effective vaccinesProtein-based vaccinesSerovar-dependentInfectious disease of global importanceImmune response dataInfectious diseasesShort-term immunityVaccine approachesDNA vaccineAt-risk populationsImmune mechanismsImmunological aspectsLeptospirosis
2018
Proteomics as a tool to understand Leptospira physiology and virulence: Recent advances, challenges and clinical implications
Nascimento Filho E, Vieira M, Teixeira A, Santos J, Fernandes L, Passalia F, Daroz B, Rossini A, Kochi L, Cavenague M, Pimenta D, Nascimento A. Proteomics as a tool to understand Leptospira physiology and virulence: Recent advances, challenges and clinical implications. Journal Of Proteomics 2018, 180: 80-87. PMID: 29501847, DOI: 10.1016/j.jprot.2018.02.025.Peer-Reviewed Original Research
2017
Automatized image processing of bovine blastocysts produced in vitro for quantitative variable determination
Rocha J, Passalia F, Matos F, Takahashi M, Maserati Jr M, Alves M, de Almeida T, Cardoso B, Basso A, Nogueira M. Automatized image processing of bovine blastocysts produced in vitro for quantitative variable determination. Scientific Data 2017, 4: 170192. PMID: 29257125, PMCID: PMC5735923, DOI: 10.1038/sdata.2017.192.Peer-Reviewed Original ResearchConceptsProcessing imagesAutomatic image processingDevelopment of artificial intelligence techniquesDigital imagesArtificial intelligence techniquesIntelligence techniquesImage processingReal-timeBovine blastocystsImage dataAutomated image processingStandard evaluationImages of blastocystsWhole blastocystImagesBlastocystMammalian embryosNon-invasive methodMultivariate modelIn vitro