David Chetrit, PhD
Associate Research ScientistAbout
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Titles
Associate Research Scientist
Education & Training
- PhD
- Tel Aviv University (2011)
Research
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Research at a Glance
Yale Co-Authors
Frequent collaborators of David Chetrit's published research.
Erdem Karatekin, PhD
Craig Roy, PhD
Derek Toomre, PhD
Sudheer Kumar Cheppali
Ane Landajuela Larma
Jun Liu, PhD
Publications
2025
In situ structures of the Legionella Dot/Icm T4SS identify the DotA–IcmX complex as the gatekeeper for effector translocation
Yue J, Heydari S, Park D, Chetrit D, Tachiyama S, Guo W, Botting J, Wu S, Roy C, Liu J. In situ structures of the Legionella Dot/Icm T4SS identify the DotA–IcmX complex as the gatekeeper for effector translocation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2025, 122: e2516300122. PMID: 40986344, PMCID: PMC12501162, DOI: 10.1073/pnas.2516300122.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsLegionella pneumophilaEffector translocationEffector proteinsCryo-FIB millingTranslocation of effector proteinsType IV secretion systemSingle-particle cryoelectron microscopyIV secretion systemCryo-ETOuter membrane porinsDot/Icm T4SSIcm proteinsNear-atomic structureSecretion systemMembrane porinsDot/IcmBacterial cytoplasmBacterial envelopeHost membraneHost cellsIntact bacteriaExtensive rearrangementCryoelectron microscopyCryoelectron tomographyInactive stateVesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1
Tsemperouli M, Cheppali S, Rivera-Molina F, Chetrit D, Landajuela A, Toomre D, Karatekin E. Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1. Biophysical Journal 2025, 124: 1878. PMID: 40010339, PMCID: PMC12256865, DOI: 10.1016/j.bpj.2025.02.013.Peer-Reviewed Original ResearchCitations
2024
Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1
Tsemperouli M, Cheppali S, Rivera-Molina F, Chetrit D, Landajuela A, Toomre D, Karatekin E. Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1. Biophysical Journal 2024, 124: 1798-1814. PMID: 39719826, PMCID: PMC12256845, DOI: 10.1016/j.bpj.2024.12.023.Peer-Reviewed Original ResearchCitationsConceptsVesicle dockingSynaptotagmin-1C2 domainFusion triggeringCalcium sensor synaptotagmin 1Neuronal SNARE complexFusion pore dynamicsSensor synaptotagmin-1Domains of Syt1Neuroendocrine cell linePolybasic patchSNARE complexTriggers exocytosisFusion poreFusion eventsDense-core vesiclesSyt1Synaptic vesiclesPlasma membranePore regulationCalcium sensorNegatively charged phospholipidsBind calciumPore dynamicsCell linesThe T4bSS of Legionella features a two-step secretion pathway with an inner membrane intermediate for secretion of transmembrane effectors
Malmsheimer S, Grin I, Bohn E, Franz-Wachtel M, Macek B, Sahr T, Smollich F, Chetrit D, Meir A, Roy C, Buchrieser C, Wagner S. The T4bSS of Legionella features a two-step secretion pathway with an inner membrane intermediate for secretion of transmembrane effectors. PLOS Pathogens 2024, 20: e1012118. PMID: 39546547, PMCID: PMC11602083, DOI: 10.1371/journal.ppat.1012118.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsEukaryotic host cellsEffector proteinsMembrane intermediatesC-terminal secretion signalHost cellsSoluble effector proteinsCytoplasmic sideBacterial inner membraneMechanism of secretionSecretion systemSecretion signalPeriplasmic loopTransmembrane effectorSecretion pathwayT4BSSInner membraneSubcellular locationIntracellular survivalMembrane targetingProtein complexesEfficient translocationBacterial cellsProteomic analysisL. pneumophilaSecretion processType IV secretion systems drive lipid mixing during substrate exchange
Chetrit D, Roy C, Karatekin E. Type IV secretion systems drive lipid mixing during substrate exchange. Biophysical Journal 2024, 123: 541a. DOI: 10.1016/j.bpj.2023.11.3276.Peer-Reviewed Original ResearchMechanisms of vesicle docking and fusion pore expansion by calcium-sensor synaptotagmin-1
Cheppali S, Tsemperouli M, Chetrit D, Rivera-Molina F, Toomre D, Karatekin E. Mechanisms of vesicle docking and fusion pore expansion by calcium-sensor synaptotagmin-1. Biophysical Journal 2024, 123: 381a. DOI: 10.1016/j.bpj.2023.11.2328.Peer-Reviewed Original Research