Alyssa Laffitte
Research
Publications
2025
The LINC complex component Kms1 and CENP-B protein Cbp1 cooperate to enforce faithful homology-directed DNA repair at the nuclear periphery in S. pombe.
Laffitte A, Lin D, Tian Y, Liu N, Lusk C, Mochrie S, King M. The LINC complex component Kms1 and CENP-B protein Cbp1 cooperate to enforce faithful homology-directed DNA repair at the nuclear periphery in S. pombe. Genes & Development 2025 PMID: 41436280, DOI: 10.1101/gad.353167.125.Peer-Reviewed Original ResearchMicrohomology-mediated end joiningNuclear peripheryHomologous recombinationFlocculin genesIntergenic recombinationEnd joiningRepetitive genomic regionsHomology-directed DNA repairProtein-coding genesSpontaneous DNA damageNonallelic sequencesDNA repair mechanismsGenic regionsS. pombeGenomic regionsIntragenic recombinationProtein familyLINC complexNuclear compartmentalizationCENP-BMutagenic end joiningFlocculinDNA repairCBP1Genes
2020
Regulated lipid synthesis and LEM2/CHMP7 jointly control nuclear envelope closure
Penfield L, Shankar R, Szentgyörgyi E, Laffitte A, Mauro MS, Audhya A, Müller-Reichert T, Bahmanyar S. Regulated lipid synthesis and LEM2/CHMP7 jointly control nuclear envelope closure. Journal Of Cell Biology 2020, 219: e201908179. PMID: 32271860, PMCID: PMC7199858, DOI: 10.1083/jcb.201908179.Peer-Reviewed Original ResearchConceptsER membraneNuclear permeability barrierESCRT-III componentsC. elegans oocytesMeiotic spindle microtubulesDe novo glycerolipid synthesisPermeability barrierGlycerolipid synthesisESCRT componentsESCRT-IIIProtein phosphataseCytoplasmic membraneSpindle microtubulesNE permeabilityMeiotic spindleLipid synthesis