Stacy Malaker
Assistant ProfessorCards
About
Research
Publications
2025
Recent Advances in Mass Spectrometry-Based Bottom-Up Proteomics
Movassaghi C, Sun J, Jiang Y, Turner N, Chang V, Chung N, Chen R, Browne E, Lin C, Schweppe D, Malaker S, Meyer J. Recent Advances in Mass Spectrometry-Based Bottom-Up Proteomics. Analytical Chemistry 2025, 97: 4728-4749. PMID: 40000226, DOI: 10.1021/acs.analchem.4c06750.Peer-Reviewed Original ResearchPNGaseF-Generated N‑Glycans Adduct onto Peptides in the Gas Phase
Rangel-Angarita V, Chongsaritsinsuk J, Mahoney K, Kim L, Chen R, Appah-Sampong A, Tran I, Steigmeyer A, Hollenhorst M, Malaker S. PNGaseF-Generated N‑Glycans Adduct onto Peptides in the Gas Phase. Journal Of The American Society For Mass Spectrometry 2025, 36: 542-552. PMID: 39938005, DOI: 10.1021/jasms.4c00431.Peer-Reviewed Original ResearchGlycoproteoforms of Osteoarthritis-associated Lubricin in Plasma and Synovial Fluid
Afshari A, Chang V, Thomsson K, Höglund J, Browne E, Karadzhov G, Mahoney K, Lucas T, Rangel-Angarita V, Ryberg H, Gidwani K, Pettersson K, Rolfson O, Björkman L, Eisler T, Schmidt T, Jay G, Malaker S, Karlsson N. Glycoproteoforms of Osteoarthritis-associated Lubricin in Plasma and Synovial Fluid. Molecular & Cellular Proteomics 2025, 24: 100923. PMID: 39922311, PMCID: PMC11925169, DOI: 10.1016/j.mcpro.2025.100923.Peer-Reviewed Original ResearchDecoding Extracellular Protein Glycosylation in Human Health and Disease.
Steigmeyer A, Lowery S, Rangel-Angarita V, Malaker S. Decoding Extracellular Protein Glycosylation in Human Health and Disease. Annual Review Of Analytical Chemistry 2025 PMID: 39813736, DOI: 10.1146/annurev-anchem-071024-124203.Peer-Reviewed Original ResearchPost-translational modificationsProtein glycosylationStudy protein glycosylationHeterogeneous post-translational modificationsBiologically relevant contextProtein foldingGlycan complexityNontemplated natureCell adhesionAberrant glycosylationProtein backboneGlycosylationBiochemical processesProteinGlycoprotein characterizationCovalent attachmentHealth and DiseaseGlycansStructural heterogeneityHuman healthRelevant contextGlycoproteinFoldingBiologyN-
2024
Unraveling O‑Glycan Diversity of Mucins: Insights from SmE Mucinase and Ultraviolet Photodissociation Mass Spectrometry
Helms A, Chang V, Malaker S, Brodbelt J. Unraveling O‑Glycan Diversity of Mucins: Insights from SmE Mucinase and Ultraviolet Photodissociation Mass Spectrometry. Analytical Chemistry 2024, 96: 19230-19237. PMID: 39576755, PMCID: PMC11653425, DOI: 10.1021/acs.analchem.4c02011.Peer-Reviewed Original ResearchUltraviolet photodissociationUltraviolet photodissociation mass spectrometryPhotodissociation mass spectrometryDomain proteinsMicroheterogeneity of glycosylationStructural characterizationMass spectrometryGlycan mappingTandem repeatsMUC-16Target proteasesPhotodissociationDiverse classSpectrometryTIM-1ProteinGlycoproteinQuantification and Site-Specific Analysis of Co-occupied N- and O‑Glycopeptides
Chongsaritsinsuk J, Rangel-Angarita V, Lucas T, Mahoney K, Enny O, Katemauswa M, Malaker S. Quantification and Site-Specific Analysis of Co-occupied N- and O‑Glycopeptides. Journal Of Proteome Research 2024, 23: 5449-5461. PMID: 39498894, DOI: 10.1021/acs.jproteome.4c00574.Peer-Reviewed Original ResearchElectron-based methodsO-glycopeptidesElectron-based fragmentation methodsFragmentation methodCo-occupancyO-glycosylated peptidesAnalysis of glycopeptidesPost-translational modificationsN-glycoproteomic analysisCore 1 structureComplex samplesO-glycansPurified proteinProtein glycosylationO-glycositesN-glycoproteomeNonmucin glycoproteinsTryptic peptidesDissociation methodO-linkedAnalysis of mucinsSite-specific analysisGlycoproteinGlycosylationProteinO-Glycoproteomics: Methods, Challenges, and New Opportunities
Riley N, Malaker S. O-Glycoproteomics: Methods, Challenges, and New Opportunities. 2024, 118-162. DOI: 10.1039/9781839166433-00118.Peer-Reviewed Original ResearchElectron transfer dissociationMass spectrometryBeam-type collision-induced dissociationSupplemental collisional activationCollision-induced dissociationSolid-phase extractionCollisional activationTransfer dissociationGlycopeptide analysisTandem MSO-glycoproteomeDissociationO-glycoproteinsSite-specific localizationGlycopeptidesEThcDO-glycansSpectrometryGlycoproteomicsSpectraElectronExpanding the repertoire of GalNAc analogues for cell-specific bioorthogonal tagging of glycoproteins
Zafar A, Sridhar S, Bineva-Todd G, Cioce A, Abdulla N, Chang V, Malaker S, Hewings D, Schumann B. Expanding the repertoire of GalNAc analogues for cell-specific bioorthogonal tagging of glycoproteins. RSC Chemical Biology 2024, 5: 1002-1009. PMID: 39238612, PMCID: PMC11369666, DOI: 10.1039/d4cb00093e.Peer-Reviewed Original ResearchAnalysis of Mucin‐Domain Glycoproteins Using Mass Spectrometry
Mahoney K, Malaker S. Analysis of Mucin‐Domain Glycoproteins Using Mass Spectrometry. Current Protocols 2024, 4: e1100. PMID: 38984456, PMCID: PMC11239139, DOI: 10.1002/cpz1.1100.Peer-Reviewed Original ResearchGlycoproteomics: Charting new territory in mass spectrometry and glycobiology
Malaker S. Glycoproteomics: Charting new territory in mass spectrometry and glycobiology. Journal Of Mass Spectrometry 2024, 59: e5034. PMID: 38726698, DOI: 10.1002/jms.5034.Peer-Reviewed Original Research
Academic Achievements & Community Involvement
News
News
- September 08, 2023
WHRY Launches New Pilot Project Studies
- May 25, 2023Source: NBC CT
Yale Researcher Studying How to Detect Ovarian Cancer Earlier
- May 16, 2023
Women’s Health Research at Yale Funds Studies to Improve Early Detection of Ovarian Cancer, Treatment of Prenatal Infections, and Aortic Aneurysm Outcomes in Women