Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2
Cardin JA, Carlén M, Meletis K, Knoblich U, Zhang F, Deisseroth K, Tsai LH, Moore CI. Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2. Nature Protocols 2010, 5: 247-254. PMID: 20134425, PMCID: PMC3655719, DOI: 10.1038/nprot.2009.228.Peer-Reviewed Original ResearchConceptsOptical interferenceMajor long-term goalViral vectorsCell-type selectivityRecording of neuronsOptogenetic stimulationChannelrhodopsin-2Cre-dependent expressionBrain circuit functionSelective cell typesInhibitory interneuronsIntracellular recordingsVivo electrophysiologyExcitatory neuronsIntact brainType selectivityNeural subtypesOptogenetic techniquesSpecific populationsNeural activityCircuit functionNeuronsInterferenceCell typesStimulation