Featured Publications
Methods of enhanced FIB-SEM sample preparation and image acquisition
Pang S, Xu C. Methods of enhanced FIB-SEM sample preparation and image acquisition. Methods In Cell Biology 2023, 177: 269-300. PMID: 37451770, DOI: 10.1016/bs.mcb.2023.01.019.ChaptersAn open-access volume electron microscopy atlas of whole cells and tissues
Xu CS, Pang S, Shtengel G, Müller A, Ritter AT, Hoffman HK, Takemura SY, Lu Z, Pasolli HA, Iyer N, Chung J, Bennett D, Weigel AV, Freeman M, van Engelenburg SB, Walther TC, Farese RV, Lippincott-Schwartz J, Mellman I, Solimena M, Hess HF. An open-access volume electron microscopy atlas of whole cells and tissues. Nature 2021, 599: 147-151. PMID: 34616045, PMCID: PMC9004664, DOI: 10.1038/s41586-021-03992-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCells, CulturedDatasets as TopicDrosophila melanogasterFemaleGolgi ApparatusHumansInformation DisseminationInterphaseIslets of LangerhansMaleMiceMicroscopy, Electron, ScanningMicrotubulesNeurogliaNeuronsOpen Access PublishingOrganellesOvarian NeoplasmsRibosomesSynaptic VesiclesT-Lymphocytes, CytotoxicConceptsDrosophila neural tissueWhole cellsThin-section electron microscopyVolume electron microscopyCellular architectureMouse pancreatic isletsCancer cellsEM tomographyCellular structureCellsCellular samplesNeural tissuePancreatic isletsEnhanced signal detectionAtlasBeam-scanning electron microscopyTissueElectron microscopyOpen access dataBiologyImmune cellsSubsequent analysisSEM scanningMicroscopyWhole-cell organelle segmentation in volume electron microscopy
Heinrich L, Bennett D, Ackerman D, Park W, Bogovic J, Eckstein N, Petruncio A, Clements J, Pang S, Xu CS, Funke J, Korff W, Hess HF, Lippincott-Schwartz J, Saalfeld S, Weigel AV. Whole-cell organelle segmentation in volume electron microscopy. Nature 2021, 599: 141-146. PMID: 34616042, DOI: 10.1038/s41586-021-03977-3.Peer-Reviewed Original ResearchConceptsAutomatic reconstructionDeep learning architectureLearning architectureWeb repositoriesOpen dataAutomatic methodThree-dimensional reconstructionSuch methodsVolume electron microscopyQueriesSegmentationRepositoryArchitectureComputer codeSpatial interactionsDatasetReconstructionImagesMetricsCodeSuch reconstructions3D FIB-SEM reconstruction of microtubule–organelle interaction in whole primary mouse β cells
Müller A, Schmidt D, Xu CS, Pang S, D’Costa J, Kretschmar S, Münster C, Kurth T, Jug F, Weigert M, Hess HF, Solimena M. 3D FIB-SEM reconstruction of microtubule–organelle interaction in whole primary mouse β cells. Journal Of Cell Biology 2020, 220: e202010039. PMID: 33326005, PMCID: PMC7748794, DOI: 10.1083/jcb.202010039.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCell NucleusCells, CulturedGlucoseImaging, Three-DimensionalInsulinInsulin-Secreting CellsMice, Inbred C57BLMicroscopy, Electron, ScanningMicrotubulesOrganellesSecretory VesiclesConceptsInsulin secretory granulesΒ-cellsSecretory granulesPrimary mammalian cellsFirst 3D reconstructionPrimary mouse β-cellsMouse β-cellsMammalian cellsMicrotubule organizationPlasma membraneIntracellular traffickingIslet β-cellsMicrotubule networkMicrotubulesUnprecedented resolutionCell constituentsMicrotubule numberCell functionGolgi apparatiCentriolesCellsEndocrine cellsGlucose stimulationEndomembranesGranules
2024
The physical and cellular mechanism of structural color change in zebrafish
Gur D, Moore A, Deis R, Song P, Wu X, Pinkas I, Deo C, Iyer N, Hess H, Hammer J, Lippincott-Schwartz J. The physical and cellular mechanism of structural color change in zebrafish. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2308531121. PMID: 38805288, PMCID: PMC11161791, DOI: 10.1073/pnas.2308531121.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsColorMicroscopy, Electron, ScanningNorepinephrinePigmentationZebrafishZebrafish ProteinsConceptsMicrotubule organizing centerCellular machineryFluorescence light microscopyOrganizing centerPharmacological perturbationsDyneinIridophoresRegulate body temperatureMicrotubulesCellular mechanismsIntracellular cAMPZebrafishMachineryIntracellular crystalsIon beam scanning electron microscopyGuanine crystalsStructural color changeLight microscopyColor change
2022
En bloc preparation of Drosophila brains enables high-throughput FIB-SEM connectomics
Lu Z, Xu C, Hayworth K, Pang S, Shinomiya K, Plaza S, Scheffer L, Rubin G, Hess H, Rivlin P, Meinertzhagen I. En bloc preparation of Drosophila brains enables high-throughput FIB-SEM connectomics. Frontiers In Neural Circuits 2022, 16: 917251. PMID: 36589862, PMCID: PMC9801301, DOI: 10.3389/fncir.2022.917251.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainConnectomeDrosophilaMicroscopy, Electron, ScanningSynapsesVolume Electron Microscopy