2024
Protocol for isolating and identifying small extracellular vesicles derived from human umbilical cord mesenchymal stem cells
Chen Y, Qian H, Mak M, Tao Z. Protocol for isolating and identifying small extracellular vesicles derived from human umbilical cord mesenchymal stem cells. STAR Protocols 2024, 5: 103197. PMID: 39028618, PMCID: PMC11315167, DOI: 10.1016/j.xpro.2024.103197.Peer-Reviewed Original ResearchSmall extracellular vesiclesUmbilical cord mesenchymal stem cellsHuman umbilical cord mesenchymal stem cellsIsolation of small extracellular vesiclesLipid bilayer-enclosed particlesExtracellular vesiclesMesenchymal stem cellsNanoparticle tracking analysisAtomic force microscopeStem cellsMolecular markersLiving cellsTransmission electron microscopyWestern blottingIsolatesVesiclesCellsForce microscopeBiophysical and biochemical aspects of immune cell–tumor microenvironment interactions
Benmelech S, Le T, McKay M, Nam J, Subramaniam K, Tellez D, Vlasak G, Mak M. Biophysical and biochemical aspects of immune cell–tumor microenvironment interactions. APL Bioengineering 2024, 8: 021502. PMID: 38572312, PMCID: PMC10990568, DOI: 10.1063/5.0195244.Peer-Reviewed Original ResearchTumor microenvironmentImprove CAR T cell therapyCAR-T cell therapyCD4+ T cellsCancer cellsAdaptive immune systemDistribution of cancer cellsCD8+T cellsImmunosuppressive responsePro-tumorImmune cellsMicroenvironment interactionsCell therapyExtracellular matrixCancer progressionImmune systemTumorCells-thatSpatial distribution of cancer cellsCancerCellsCD8CD4Therapy
2023
Fiber alignment in 3D collagen networks as a biophysical marker for cell contractility
Böhringer D, Bauer A, Moravec I, Bischof L, Kah D, Mark C, Grundy T, Görlach E, O'Neill G, Budday S, Strissel P, Strick R, Malandrino A, Gerum R, Mak M, Rausch M, Fabry B. Fiber alignment in 3D collagen networks as a biophysical marker for cell contractility. Matrix Biology 2023, 124: 39-48. PMID: 37967726, PMCID: PMC10872942, DOI: 10.1016/j.matbio.2023.11.004.Peer-Reviewed Original ResearchConceptsCellular traction forcesTraction forceHepatic stellate cellsMatrix remodelingGlioblastoma cell linesFiber orientationCell contractilityDrug screening assaysLocal fiber orientationCell forcesRho-kinaseFiber alignmentStellate cellsCell linesScreening assayFiber networkCellsPython packageMechanical propertiesForce reconstructionBiopolymer matrixRemodelingInduced deformationQualitative proxyCollagen network