1996
Functional expression of the cDNA encoded by the human ATP1AL1 gene
Grishin AV, Bevensee MO, Modyanov NN, Rajendran V, Boron WF, Caplan MJ. Functional expression of the cDNA encoded by the human ATP1AL1 gene. American Journal Of Physiology 1996, 271: f539-f551. PMID: 8853415, DOI: 10.1152/ajprenal.1996.271.3.f539.Peer-Reviewed Original ResearchConceptsHuman ATP1AL1 geneAcute loweringNH4Cl pulseUptake activityHEK-293 cellsSpontaneous intracellularMM ouabainProtein expressionCOS cellsATP1AL1Polyclonal antibodiesPH-sensitive dyeOuabainBeta-subunit cDNAExtrusion activityCellsK-ATPaseTransfection studiesSame cellsFunctional expressionBeta complexExpressionDependent ATPaseEndplasmic reticulumProtein
1986
Newly synthesized Na,K-ATPase alpha-subunit has no cytosolic intermediate in MDCK cells.
Caplan MJ, Palade GE, Jamieson JD. Newly synthesized Na,K-ATPase alpha-subunit has no cytosolic intermediate in MDCK cells. Journal Of Biological Chemistry 1986, 261: 2860-2865. PMID: 3005269, DOI: 10.1016/s0021-9258(17)35866-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDogsKidneyMacromolecular SubstancesMathematicsMethionineMiceSodium-Potassium-Exchanging ATPaseConceptsAlpha subunitAnimal cell plasma membranesK-ATPase alpha subunitMDCK cellsMembrane-bound vesiclesCell plasma membraneCytosol fractionTransmembrane proteinCrude cytosolic fractionPlasma membraneMembranous vesiclesPrecursor-product relationshipSoluble precursorsMembrane vesiclesK-ATPaseCytosolic fractionMembrane pelletVesiclesStaphylococcus aureus cellsAureus cellsCellsMonoclonal antibodiesProteinPrecursorsMembrane