Featured Publications
Motion of VAPB molecules reveals ER–mitochondria contact site subdomains
Obara C, Nixon-Abell J, Moore A, Riccio F, Hoffman D, Shtengel G, Xu C, Schaefer K, Pasolli H, Masson J, Hess H, Calderon C, Blackstone C, Lippincott-Schwartz J. Motion of VAPB molecules reveals ER–mitochondria contact site subdomains. Nature 2024, 626: 169-176. PMID: 38267577, PMCID: PMC10830423, DOI: 10.1038/s41586-023-06956-y.Peer-Reviewed Original ResearchConceptsContact sitesExchange of signaling moleculesInterorganelle communicationOrganelle tetheringEukaryotic cellsSingle-molecule imagingCellular physiologyThree-dimensional electron microscopyMembrane curvatureSignaling moleculesExchange of moleculesDynamic subdomainsNanoscale organizationProtein BMetabolic needsSubdomainsCellsSitesMutationsMoleculesRemodelingSites1,2HomeostasisCommunication hubRegulation
2022
The 3D ultrastructure of the chordotonal organs in the antenna of a microwasp remains complex although simplified
Diakova A, Makarova A, Pang S, Xu C, Hess H, Polilov A. The 3D ultrastructure of the chordotonal organs in the antenna of a microwasp remains complex although simplified. Scientific Reports 2022, 12: 20172. PMID: 36424494, PMCID: PMC9691716, DOI: 10.1038/s41598-022-24390-4.Peer-Reviewed Original Research
2017
Diverse protocols for correlative super-resolution fluorescence imaging and electron microscopy of chemically fixed samples
Kopek BG, Paez-Segala MG, Shtengel G, Sochacki KA, Sun MG, Wang Y, Xu CS, van Engelenburg SB, Taraska JW, Looger LL, Hess HF. Diverse protocols for correlative super-resolution fluorescence imaging and electron microscopy of chemically fixed samples. Nature Protocols 2017, 12: 916-946. PMID: 28384138, PMCID: PMC5514615, DOI: 10.1038/nprot.2017.017.Peer-Reviewed Original Research
2016
Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER
Nixon-Abell J, Obara CJ, Weigel AV, Li D, Legant WR, Xu CS, Pasolli HA, Harvey K, Hess HF, Betzig E, Blackstone C, Lippincott-Schwartz J. Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER. Science 2016, 354: aaf3928-aaf3928. PMID: 27789813, PMCID: PMC6528812, DOI: 10.1126/science.aaf3928.Peer-Reviewed Original Research
2012
Correlative 3D superresolution fluorescence and electron microscopy reveal the relationship of mitochondrial nucleoids to membranes
Kopek BG, Shtengel G, Xu CS, Clayton DA, Hess HF. Correlative 3D superresolution fluorescence and electron microscopy reveal the relationship of mitochondrial nucleoids to membranes. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 6136-6141. PMID: 22474357, PMCID: PMC3341004, DOI: 10.1073/pnas.1121558109.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsDNA, MitochondrialDNA-Binding ProteinsFibroblastsHigh Mobility Group ProteinsImaging, Three-DimensionalLuminescent ProteinsMiceMicroscopy, ElectronMicroscopy, FluorescenceMicroscopy, InterferenceMitochondriaMitochondrial MembranesMitochondrial ProteinsReproducibility of ResultsConceptsMitochondrial nucleoidsSuperresolution fluorescenceIon beam ablationOptical microscopy techniquesFluorescent protein labelsCellular contextUltrastructural contextDirect imagingBiological questionsBiological processesSpecific proteinsCellular ultrastructureSuperresolution microscopyProtein labelCellular architectureFluorescence microscopyLocalization microscopyEM imagesNucleoidsMicroscopy techniquesProteinSample preparation protocolElectron microscopyVivo natureMicroscopy