Venkata Lakshmi Manasa Chanduri
Associate Research ScientistCards
About
Research
Publications
2024
Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis
Chanduri M, Kumar A, Weiss D, Emuna N, Barsukov I, Shi M, Tanaka K, Wang X, Datye A, Kanyo J, Collin F, Lam T, Schwarz U, Bai S, Nottoli T, Goult B, Humphrey J, Schwartz M. Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis. Science Advances 2024, 10: eadi6286. PMID: 39167642, PMCID: PMC11338229, DOI: 10.1126/sciadv.adi6286.Peer-Reviewed Original ResearchConceptsTissue mechanical homeostasisStiffness sensingExtracellular matrixTalin-1Mechanical homeostasisExtracellular matrix mechanicsIncreased cell spreadingCell spreadingTalinMutationsCellular sensingFibrillar collagenReduced axial stiffnessTissue mechanical propertiesMechanical propertiesAxial stiffnessCompliant substratesHomeostasisRupture pressureArp2/3ARPC5LStiffnessHomeostasis hypothesisResident cellsTissue stiffnessMechanosensing through talin 1 contributes to tissue mechanical homeostasis.
Chanduri MVL, Kumar A, Weiss D, Emuna N, Barsukov I, Shi M, Tanaka K, Wang X, Datye A, Kanyo J, Collin F, Lam T, Schwarz UD, Bai S, Nottoli T, Goult BT, Humphrey JD, Schwartz MA. Mechanosensing through talin 1 contributes to tissue mechanical homeostasis. BioRxiv 2024 PMID: 38328095, DOI: 10.1101/2023.09.03.556084.Peer-Reviewed Original Research In Press
2023
TLN1 contains a cancer-associated cassette exon that alters talin-1 mechanosensitivity
Gallego-Paez L, Edwards W, Chanduri M, Guo Y, Koorman T, Lee C, Grexa N, Derksen P, Yan J, Schwartz M, Mauer J, Goult B. TLN1 contains a cancer-associated cassette exon that alters talin-1 mechanosensitivity. Journal Of Cell Biology 2023, 222: e202209010. PMID: 36880935, PMCID: PMC9997659, DOI: 10.1083/jcb.202209010.Peer-Reviewed Original ResearchConceptsExon 17bTerminal FERM domainVinculin bindingFERM domainSwitch domainAdhesion dynamicsCassette exonsSplicing analysisAdapter proteinTLN1Single isoformIsoform switchTalin-1Amino acidsFrame insertionExonsBiochemical analysisIsoformsProteinExon 17CytoskeletonGenesMechanotransductionDomainIntegrins
2020
Back-Pyrophosphorylation Assay to Detect In Vivo InsP(7)-Dependent Protein Pyrophosphorylation in Mammalian Cells.
Chanduri M, Bhandari R. Back-Pyrophosphorylation Assay to Detect In Vivo InsP(7)-Dependent Protein Pyrophosphorylation in Mammalian Cells. Methods Mol Biol 2020, 2091: 93-105. PMID: 31773573, DOI: 10.1007/978-1-0716-0167-9_8.Chapters
2016
Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport.
Chanduri M, Rai A, Malla AB, Wu M, Fiedler D, Mallik R, Bhandari R. Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport. Biochem J 2016, 473: 3031-47. PMID: 27474409, DOI: 10.1042/BCJ20160610.Peer-Reviewed Original Research
2013
Inositol pyrophosphate synthesis by inositol hexakisphosphate kinase 1 is required for homologous recombination repair.
Jadav RS, Chanduri MV, Sengupta S, Bhandari R. Inositol pyrophosphate synthesis by inositol hexakisphosphate kinase 1 is required for homologous recombination repair. J Biol Chem 2013, 288: 3312-21. PMID: 23255604, DOI: 10.1074/jbc.M112.396556.Peer-Reviewed Original Research