2025
Reproducibility of the Determination of 13C‐Labeling of Glutamate and Glutamine in the Human Brain Using selPOCE‐MRS at 7 T Upon [U‐13C]–Labeled Glucose Infusion
Ahmadian N, Jacobs S, Gosselink M, van der Kemp W, Hoogduin H, Coppoli A, Mason G, de Graaf R, Norouzizadeh H, Mahon C, van Eijsden P, Tiessen R, Cerneus D, Miller C, De Lepeleire I, Basile A, Klomp D, Prompers J, Wiegers E. Reproducibility of the Determination of 13C‐Labeling of Glutamate and Glutamine in the Human Brain Using selPOCE‐MRS at 7 T Upon [U‐13C]–Labeled Glucose Infusion. NMR In Biomedicine 2025, 38: e70026. PMID: 40204526, PMCID: PMC11981885, DOI: 10.1002/nbm.70026.Peer-Reviewed Original Research
2022
Rates of pyruvate carboxylase, glutamate and GABA neurotransmitter cycling, and glucose oxidation in multiple brain regions of the awake rat using a combination of [2-13C]/[1-13C]glucose infusion and 1H-[13C]NMR ex vivo
McNair LM, Mason GF, Chowdhury GM, Jiang L, Ma X, Rothman DL, Waagepetersen HS, Behar KL. Rates of pyruvate carboxylase, glutamate and GABA neurotransmitter cycling, and glucose oxidation in multiple brain regions of the awake rat using a combination of [2-13C]/[1-13C]glucose infusion and 1H-[13C]NMR ex vivo. Cerebrovascular And Brain Metabolism Reviews 2022, 42: 1507-1523. PMID: 35048735, PMCID: PMC9274856, DOI: 10.1177/0271678x221074211.Peer-Reviewed Original ResearchConceptsCerebral cortexThree-compartment metabolic modelBrain regionsAwake male ratsTime courseMultiple brain regionsAwake ratsMale ratsAwake humansNeurotransmitter cyclingBrain rateAnesthetized rodentsEx vivoRate of glutamateCortexHippocampusStriatumGlutamateRatsBlood plasmaCerebellumGlucose consumptionGlucose oxidationRapid euthanasiaCortical data
2020
Characterization of Kinetic Isotope Effects and Label Loss in Deuterium-Based Isotopic Labeling Studies
de Graaf RA, Thomas MA, Behar KL, De Feyter HM. Characterization of Kinetic Isotope Effects and Label Loss in Deuterium-Based Isotopic Labeling Studies. ACS Chemical Neuroscience 2020, 12: 234-243. PMID: 33319987, PMCID: PMC9890388, DOI: 10.1021/acschemneuro.0c00711.Peer-Reviewed Original Research
2019
Liver retinol estimated by 13C-retinol isotope dilution at 7 versus 14 days in Burkinabe schoolchildren
Bationo J, Zeba A, Coulibaly N, Sheftel J, Davis C, Bassole I, Barro N, Ouedraogo J, Tanumihardjo S. Liver retinol estimated by 13C-retinol isotope dilution at 7 versus 14 days in Burkinabe schoolchildren. Experimental Biology And Medicine 2019, 244: 1430-1437. PMID: 31547685, PMCID: PMC6900701, DOI: 10.1177/1535370219877132.Peer-Reviewed Original ResearchConceptsBody surface areaTotal liver vitaminBlood drawLiver massBody weightDay 7Liver vitaminLiver weightDay 14TLR valuesBaseline blood drawSerum retinol concentrationsTotal-body vitaminTotal body storesDilution testingRetinol isotope dilutionPublic health programsLiver reserveLiver biopsyLiver retinolRetinol concentrationsSchool-aged childrenAdequate vitaminTracer doseBlood samplesIn vivo 13C and 1H‐[13C] MRS studies of neuroenergetics and neurotransmitter cycling, applications to neurological and psychiatric disease and brain cancer
Rothman DL, de Graaf R, Hyder F, Mason GF, Behar KL, De Feyter HM. In vivo 13C and 1H‐[13C] MRS studies of neuroenergetics and neurotransmitter cycling, applications to neurological and psychiatric disease and brain cancer. NMR In Biomedicine 2019, 32: e4172. PMID: 31478594, DOI: 10.1002/nbm.4172.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2017
Non-invasive assessment of hepatic mitochondrial metabolism by positional isotopomer NMR tracer analysis (PINTA)
Perry RJ, Peng L, Cline GW, Butrico GM, Wang Y, Zhang XM, Rothman DL, Petersen KF, Shulman GI. Non-invasive assessment of hepatic mitochondrial metabolism by positional isotopomer NMR tracer analysis (PINTA). Nature Communications 2017, 8: 798. PMID: 28986525, PMCID: PMC5630596, DOI: 10.1038/s41467-017-01143-w.Peer-Reviewed Original ResearchConceptsMitochondrial metabolismHepatic mitochondrial metabolismPyruvate carboxylase fluxCitrate synthase fluxPyruvate cyclingMitochondrial uncouplerIntermediary metabolismSpectrometry analysisPhysiological conditionsChromatography-mass spectrometry analysisSynthase fluxCentral roleMetabolismHepatic mitochondriaGas chromatography-mass spectrometry analysisVivo NMR spectroscopyMitochondriaNMR spectroscopyRegulationUncouplerRoleTracer analysisVivoMaintenance of normoglycemiaWide rangeIn vivo proton observed carbon edited (POCE) 13C magnetic resonance spectroscopy of the rat brain using a volumetric transmitter and receive‐only surface coil on the proton channel
Kumaragamage C, Madularu D, Mathieu AP, De Feyter H, Rajah MN, Near J. In vivo proton observed carbon edited (POCE) 13C magnetic resonance spectroscopy of the rat brain using a volumetric transmitter and receive‐only surface coil on the proton channel. Magnetic Resonance In Medicine 2017, 79: 628-635. PMID: 28497464, DOI: 10.1002/mrm.26751.Peer-Reviewed Original Research
2015
Integrated, Step-Wise, Mass-Isotopomeric Flux Analysis of the TCA Cycle
Alves TC, Pongratz RL, Zhao X, Yarborough O, Sereda S, Shirihai O, Cline GW, Mason G, Kibbey RG. Integrated, Step-Wise, Mass-Isotopomeric Flux Analysis of the TCA Cycle. Cell Metabolism 2015, 22: 936-947. PMID: 26411341, PMCID: PMC4635072, DOI: 10.1016/j.cmet.2015.08.021.Peer-Reviewed Original ResearchImaging the Cannabinoid CB1 Receptor in Humans with [11C] OMAR: Assessment of Kinetic Analysis Methods, Test–Retest Reproducibility, and Gender Differences
Normandin MD, Zheng MQ, Lin KS, Mason NS, Lin SF, Ropchan J, Labaree D, Henry S, Williams WA, Carson RE, Neumeister A, Huang Y. Imaging the Cannabinoid CB1 Receptor in Humans with [11C] OMAR: Assessment of Kinetic Analysis Methods, Test–Retest Reproducibility, and Gender Differences. Cerebrovascular And Brain Metabolism Reviews 2015, 35: 1313-1322. PMID: 25833345, PMCID: PMC4528005, DOI: 10.1038/jcbfm.2015.46.Peer-Reviewed Original ResearchConceptsTest-retest reliabilityCannabinoid type 1 receptorType 1 receptorCannabinoid CB1 receptorsPositron emission tomography (PET) imagingTest-retest reproducibilityEmission Tomography ImagingGender differencesTwo-tissue compartment modelCerebral uptakePresent studyCB1 receptorsCB1R availabilityInjected doseVascular componentBody weightReceptor availabilityNeuropsychiatric disordersMultilinear analysis methodRegional volumesReceptor bindingTomography imagingParent fractionOne-tissueHuman subjects
2014
Glutamate Metabolism in Major Depressive Disorder
Abdallah CG, Jiang L, De Feyter HM, Fasula M, Krystal JH, Rothman DL, Mason GF, Sanacora G. Glutamate Metabolism in Major Depressive Disorder. American Journal Of Psychiatry 2014, 171: 1320-1327. PMID: 25073688, PMCID: PMC4472484, DOI: 10.1176/appi.ajp.2014.14010067.Peer-Reviewed Original Research
2013
Association of Posttraumatic Stress Disorder With Reduced In Vivo Norepinephrine Transporter Availability in the Locus Coeruleus
Pietrzak RH, Gallezot JD, Ding YS, Henry S, Potenza MN, Southwick SM, Krystal JH, Carson RE, Neumeister A. Association of Posttraumatic Stress Disorder With Reduced In Vivo Norepinephrine Transporter Availability in the Locus Coeruleus. JAMA Psychiatry 2013, 70: 1199-1205. PMID: 24048210, PMCID: PMC4026855, DOI: 10.1001/jamapsychiatry.2013.399.Peer-Reviewed Original ResearchConceptsPosttraumatic stress disorderNorepinephrine transporter availabilityLocus coeruleusPTSD groupTransporter availabilityHC groupVeterans Affairs Medical CenterStress disorderPTSD symptomsClinician-Administered PTSD ScalePositron emission tomography studyEmission tomography studiesPositron emission tomographyAnxious arousal symptomsNoradrenergic dysfunctionMedical CenterStudy groupMAIN OUTCOMEPTSD symptom clustersPTSD ScaleHealthy adultsCoeruleusBrain regionsChronic stressEmission tomographyIn vivo characterization of the early states of the amyloid-beta network
Sepulcre J, Sabuncu M, Becker A, Sperling R, Johnson K. In vivo characterization of the early states of the amyloid-beta network. Brain 2013, 136: 2239-2252. PMID: 23801740, PMCID: PMC3692037, DOI: 10.1093/brain/awt146.Peer-Reviewed Original ResearchConceptsAmyloid-bAlzheimer's diseasePittsburgh compound B positron emission tomography imagingPrecuneus/posterior cingulate cortexMedial temporal lobeRegional brain atrophyAlzheimer's disease pathologyAmyloid-betaOrbitofrontal cortexCingulate cortexAmygdala nucleiTemporal lobeCortical regionsAssociated with accumulationHub propertiesEmission Tomography ImagingNeurodegenerative diseasesBrain atrophyDisease pathologyAlzheimerHippocampusAbnormal accumulationCortexIn vivo characterizationElderly subjectsCortical energy demands of signaling and nonsignaling components in brain are conserved across mammalian species and activity levels
Hyder F, Rothman DL, Bennett MR. Cortical energy demands of signaling and nonsignaling components in brain are conserved across mammalian species and activity levels. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 3549-3554. PMID: 23319606, PMCID: PMC3587194, DOI: 10.1073/pnas.1214912110.Peer-Reviewed Original ResearchConceptsGlucose oxidation ratesCerebral cortexNeuronal activityActivity levelsGlucose metabolic dataHuman cerebral cortexMetabolic dataOxidative demandAwake valuesCortical neuronsSynaptic transmissionGlucose metabolismAwake stateSensory stimulationAnatomic dataRatsCortexMammalian speciesIsoelectricityMagnetic resonance spectroscopy resultsSignaling requirementsAstrocytesCell membraneLevelsNeurons
2011
1H-[13C]-Nuclear Magnetic Resonance Spectroscopy Measures of Ketamine's Effect on Amino Acid Neurotransmitter Metabolism
Chowdhury GM, Behar KL, Cho W, Thomas MA, Rothman DL, Sanacora G. 1H-[13C]-Nuclear Magnetic Resonance Spectroscopy Measures of Ketamine's Effect on Amino Acid Neurotransmitter Metabolism. Biological Psychiatry 2011, 71: 1022-1025. PMID: 22169441, PMCID: PMC3660962, DOI: 10.1016/j.biopsych.2011.11.006.Peer-Reviewed Original ResearchConceptsMedial prefrontal cortexSubanesthetic dosesAcute effectsNeurotransmitter cyclingMagnetic resonance spectroscopy measuresMPFC of ratsPotential physiological processesAntidepressant-like propertiesSaline-treated animalsAmino acid neurotransmittersHippocampus of ratsDose of ketamineEffects of ketamineGlial energy metabolismMPFC levelsGlutamate releaseKetamine effectsΓ-aminobutyric acidGlial metabolismKetaminePrefrontal cortexEnrichment of glutamateVivo studiesEnergy metabolismRats13C MRS studies of neuroenergetics and neurotransmitter cycling in humans
Rothman DL, De Feyter HM, de Graaf R, Mason GF, Behar KL. 13C MRS studies of neuroenergetics and neurotransmitter cycling in humans. NMR In Biomedicine 2011, 24: 943-957. PMID: 21882281, PMCID: PMC3651027, DOI: 10.1002/nbm.1772.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsNeurotransmitter cyclingMRS studiesHigh neuronal activityHuman brain diseasesOnly noninvasive methodBrain metabolismGlutamate neurotransmissionNeuronal activityPsychiatric diseasesNeuronal functionBrain diseasesBrain functionClinical researchNeuroenergeticsNoninvasive methodMRSMRS measurementsHuman researchDiseaseBrain statesRecent findingsImportant new informationHumansMetabolic pathwaysNeurotransmissionStriatal dopamine transporter availability in unmedicated bipolar disorder
Anand A, Barkay G, Dzemidzic M, Albrecht D, Karne H, Zheng Q, Hutchins G, Normandin M, Yoder K. Striatal dopamine transporter availability in unmedicated bipolar disorder. Bipolar Disorders 2011, 13: 406-413. PMID: 21843280, DOI: 10.1111/j.1399-5618.2011.00936.x.Peer-Reviewed Original ResearchConceptsDopamine transporter availabilityBipolar disorderDopamine transporterPositron emission tomographyNeuropathology of bipolar disorderStriatal dopamine transporter availabilityEtiology of bipolar disorderMultilinear reference tissue modelUnmedicated BPD patientsBilateral dorsal caudatePresynaptic dopamine functionStructural magnetic resonance imagingStriatal binding potentialReceptor imaging studiesReference tissue modelMagnetic resonance imagingBPD patientsDopamine functionDopamine neurotransmissionDopamine systemDorsal caudateSynaptic dopamineBPD subjectsDopaminergic systemDepressive phaseImaging of alcohol‐induced dopamine release in rats:Preliminary findings with [11C]raclopride PET
Sullivan JM, Risacher SL, Normandin MD, Yoder KK, Froehlich JC, Morris ED. Imaging of alcohol‐induced dopamine release in rats:Preliminary findings with [11C]raclopride PET. Synapse 2011, 65: 929-937. PMID: 21308803, PMCID: PMC3130821, DOI: 10.1002/syn.20921.Peer-Reviewed Original ResearchConceptsPositron emission tomographyBlood alcohol concentrationSmall animal positron emission tomographyRat striatumAnimal positron emission tomographyP ratsAlcohol-induced dopamine releaseSignificant alcohol-induced decreaseAlcohol-induced decreaseBlood alcohol levelsLateral saphenous veinAlcohol-preferring ratsAnimals 10 minSystemic alcoholDA releaseMicrodialysis studyStriatal dopamineAlcohol administrationSaphenous veinMale WistarDopamine releaseLarge cohortExtracellular dopamineBlood samplesReceptor availabilityCharacterization of Short-Lived Electrophilic Metabolites of the Anticancer Agent Laromustine (VNP40101M)
Nassar A, King I, Du J. Characterization of Short-Lived Electrophilic Metabolites of the Anticancer Agent Laromustine (VNP40101M). Chemical Research In Toxicology 2011, 24: 568-578. PMID: 21361357, DOI: 10.1021/tx100453t.Peer-Reviewed Original ResearchConceptsHuman liver microsomesNuclear magnetic resonance spectroscopyPhase II conjugatesN-acetylecysteinePooled human liver microsomesFragment ionsLiver microsomesPhase 1 metabolismCofactor nicotinamide adenine dinucleotide phosphateReactive intermediatesMoietyElectrophilic metabolitesMagnetic resonance spectroscopyM-4MethylformamidePhysiological pHVNP40101MResonance spectroscopyLaromustineConjugateActive moietyRearrangementAlkylating agentsH-DCysteine
2010
Evaluation of Cerebral Acetate Transport and Metabolic Rates in the Rat Brain in vivo Using 1H-[13C]-NMR
Patel AB, de Graaf RA, Rothman DL, Behar KL, Mason GF. Evaluation of Cerebral Acetate Transport and Metabolic Rates in the Rat Brain in vivo Using 1H-[13C]-NMR. Cerebrovascular And Brain Metabolism Reviews 2010, 30: 1200-1213. PMID: 20125180, PMCID: PMC2879471, DOI: 10.1038/jcbfm.2010.2.Peer-Reviewed Original Research
2009
In vivo neurochemical profiling of rat brain by 1H‐[13C] NMR spectroscopy: cerebral energetics and glutamatergic/GABAergic neurotransmission
Van Eijsden P, Behar KL, Mason GF, Braun KP, De Graaf RA. In vivo neurochemical profiling of rat brain by 1H‐[13C] NMR spectroscopy: cerebral energetics and glutamatergic/GABAergic neurotransmission. Journal Of Neurochemistry 2009, 112: 24-33. PMID: 19818103, PMCID: PMC2843425, DOI: 10.1111/j.1471-4159.2009.06428.x.Peer-Reviewed Original ResearchConceptsGABAergic neurotransmissionRat brainGlutamatergic/GABAergic neurotransmissionLow GABA concentrationsInhibitory neurotransmissionGlutamatergic neurotransmissionCerebral energeticsGABA concentrationNeurotransmissionBrain functionNeurotransmitter fluxEnergy metabolismNeurochemical profilingVivoBrainHigh magnetic fieldsGABAergicExcitatoryMagnetic field
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