2025
Lipid Dynamics at Membrane Contact Sites
Reinisch K, De Camilli P, Melia T. Lipid Dynamics at Membrane Contact Sites. Annual Review Of Biochemistry 2025, 94: 479-502. PMID: 40067957, DOI: 10.1146/annurev-biochem-083024-122821.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCarrier ProteinsCell MembraneEndoplasmic ReticulumHumansLipid MetabolismMembrane LipidsMembrane ProteinsConceptsContact sitesOrganelle contact sitesMembrane contact sitesIntegral membrane proteinsLipid transfer proteinsVesicular traffickingEndoplasmic reticulumLipid transferMembrane proteinsLipid movementOrganellesLipid transportTransfer proteinCellular membranesProteinBilayer asymmetryLipid dynamicsShedding new lightLipidMembranePhysiological mechanismsEukaryotesSitesReticulumTraffickingThe pathways of secretory cargo export at the endoplasmic reticulum
Malhotra V. The pathways of secretory cargo export at the endoplasmic reticulum. Nature Communications 2025, 16: 2138. PMID: 40032897, PMCID: PMC11876584, DOI: 10.1038/s41467-025-57408-2.Peer-Reviewed Original ResearchStructural basis for the transport and regulation mechanism of the multidrug resistance-associated protein 2
Koide E, Pietz H, Beltran J, Chen J. Structural basis for the transport and regulation mechanism of the multidrug resistance-associated protein 2. Nature Communications 2025, 16: 484. PMID: 39779684, PMCID: PMC11711199, DOI: 10.1038/s41467-024-55810-w.Peer-Reviewed Original ResearchConceptsAutoinhibited stateR domainPost-translocation stateSubstrate-binding sitePre-translocation stateATP-binding siteProtein 2Nucleotide-binding domain 2Cryogenic electron microscopyStructural basisDiverse array of compoundsDomain 2Cryo-EMRegulation mechanismChemotherapeutic resistanceConformational changesMultidrug resistanceArray of compoundsDiverse arrayConformational statesLiver homeostasisMultidrug resistance-associated protein 2Initial transportSubstrateCytosol
2024
Cilia structure and intraflagellar transport differentially regulate sensory response dynamics within and between C. elegans chemosensory neurons
Philbrook A, O’Donnell M, Grunenkovaite L, Sengupta P. Cilia structure and intraflagellar transport differentially regulate sensory response dynamics within and between C. elegans chemosensory neurons. PLOS Biology 2024, 22: e3002892. PMID: 39591402, PMCID: PMC11593760, DOI: 10.1371/journal.pbio.3002892.Peer-Reviewed Original ResearchConceptsIntraflagellar transportOrganization of signaling moleculesASH nociceptive neuronsAWA olfactory neuronsCiliary traffickingNociceptive neuronsCilia baseOlfactory neuronsCaenorhabditis elegansOdor responsesSignaling proteinsCilia organizationSensory neuronsCilium structurePrimary ciliaCilia structureSignaling moleculesAcute inhibitionSegregation of receptorsRegulation of responsesChemosensory neuronsNeuron typesCilium lengthNeuronal responsesEctopic branchingA complex of the lipid transport ER proteins TMEM24 and C2CD2 with band 4.1 at cell–cell contacts
Johnson B, Iuliano M, Lam T, Biederer T, De Camilli P. A complex of the lipid transport ER proteins TMEM24 and C2CD2 with band 4.1 at cell–cell contacts. Journal Of Cell Biology 2024, 223: e202311137. PMID: 39158698, PMCID: PMC11334333, DOI: 10.1083/jcb.202311137.Peer-Reviewed Original ResearchConceptsPlasma membraneNon-vesicular lipid transferSites of cell contactC-terminus motifsCell contact-dependent signalsContact-dependent signalingCell-cell contactER/PM junctionsTMEM24ER proteinsPM proteinsSynCAM 1Cell adhesion moleculesCellular functionsLipid transferC2CD2Phospholipid transportLipid transportCell contactProteinAdhesion moleculesCalcium homeostasisCellsFamily membersParalogsElucidating a new path of CSF transport in the CNS
Benveniste H, Thomas J. Elucidating a new path of CSF transport in the CNS. The Lancet Neurology 2024, 23: 553-554. PMID: 38760087, PMCID: PMC11700482, DOI: 10.1016/s1474-4422(24)00164-9.Peer-Reviewed Original ResearchPla2g12b drives expansion of triglyceride-rich lipoproteins
Thierer J, Foresti O, Yadav P, Wilson M, Moll T, Shen M, Busch-Nentwich E, Morash M, Mohlke K, Rawls J, Malhotra V, Hussain M, Farber S. Pla2g12b drives expansion of triglyceride-rich lipoproteins. Nature Communications 2024, 15: 2095. PMID: 38453914, PMCID: PMC10920679, DOI: 10.1038/s41467-024-46102-4.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumAccumulation of intracellular lipidsSubcellular localizationTriglyceride-rich lipoproteinsEvolutionary tradeoffsInteraction partnersNascent lipoproteinsFunctional domainsLipoprotein homeostasisPLA2G12BIntracellular lipidsResistant to atherosclerosisMutant miceVertebratesReticulumTriglyceride incorporationLipid secretionCardiovascular disease riskCalcium-dependentExcessive accumulationDisease riskTriglyceride transportLipoproteinTriglyceridesLipidFluoride transport in Arabidopsis thaliana plants is impaired in Fluoride EXporter (FEX) mutants
Tausta S, Fontaine K, Hillmer A, Strobel S. Fluoride transport in Arabidopsis thaliana plants is impaired in Fluoride EXporter (FEX) mutants. Plant Molecular Biology 2024, 114: 17. PMID: 38342783, PMCID: PMC10859346, DOI: 10.1007/s11103-023-01413-w.Peer-Reviewed Original ResearchConceptsFluoride exportMutant plantsWT plantsArabidopsis thaliana plantsSingle cell eukaryotesMulticellular plantsThaliana plantsCell eukaryotesFluoride toleranceTerminal tissuesMutantsFluoride transportPlantsFloral stemsWildtypeArabidopsisEukaryotesEnvironmental toxinsStemBacteriaPositron emission tomographyPrimary locusToxinCalcium flow at ER-TGN contact sites facilitates secretory cargo export
Ramazanov B, Parchure A, Di Martino R, Kumar A, Chung M, Kim Y, Griesbeck O, Schwartz M, Luini A, von Blume J. Calcium flow at ER-TGN contact sites facilitates secretory cargo export. Molecular Biology Of The Cell 2024, 35: ar50. PMID: 38294859, PMCID: PMC11064664, DOI: 10.1091/mbc.e23-03-0099.Peer-Reviewed Original ResearchMeSH KeywordsBiological TransportCalciumCarrier ProteinsEndoplasmic ReticulumProtein TransportProteinstrans-Golgi NetworkUnbalanced regularized optimal mass transport with applications to fluid flows in the brain
Chen X, Benveniste H, Tannenbaum A. Unbalanced regularized optimal mass transport with applications to fluid flows in the brain. Scientific Reports 2024, 14: 1111. PMID: 38212659, PMCID: PMC10784574, DOI: 10.1038/s41598-023-50874-y.Peer-Reviewed Original ResearchConceptsFluid flowMass transportEntire transport processNumerical solution procedureKinetic energyTotal kinetic energyImage trackingSolution procedureOptimal mass transportTransport processesMass transport approachTransport problemsMass configurationConstraint equationsFlowTransport approachApplicationsBenamouFormulationTransportTrackingDirect measurement of dynamic attractant gradients reveals breakdown of the Patlak–Keller–Segel chemotaxis model
Phan T, Mattingly H, Vo L, Marvin J, Looger L, Emonet T. Direct measurement of dynamic attractant gradients reveals breakdown of the Patlak–Keller–Segel chemotaxis model. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2309251121. PMID: 38194458, PMCID: PMC10801886, DOI: 10.1073/pnas.2309251121.Peer-Reviewed Original Research
2023
A model for collagen secretion by intercompartmental continuities
Bunel L, Pincet L, Malhotra V, Raote I, Pincet F. A model for collagen secretion by intercompartmental continuities. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 121: e2310404120. PMID: 38147551, PMCID: PMC10769856, DOI: 10.1073/pnas.2310404120.Peer-Reviewed Original ResearchNicotine flux as a powerful tool for regulating nicotine delivery from e-cigarettes: Protocol of two complimentary randomized crossover clinical trials
El-Hellani A, Hanna E, Sharma M, Blohowiak R, Joseph P, Eid T, Nadim H, El-Hage R, Salman R, Karaoghlanian N, Adeniji A, Salam S, Talih F, Elbejjani M, Breland A, Eissenberg T, Shihadeh A, Baldassarri S, Talih S. Nicotine flux as a powerful tool for regulating nicotine delivery from e-cigarettes: Protocol of two complimentary randomized crossover clinical trials. PLOS ONE 2023, 18: e0291786. PMID: 37733666, PMCID: PMC10513228, DOI: 10.1371/journal.pone.0291786.Peer-Reviewed Original ResearchTurbocharging synaptic transmission
Rothman J, Grushin K, Bera M, Pincet F. Turbocharging synaptic transmission. FEBS Letters 2023, 597: 2233-2249. PMID: 37643878, DOI: 10.1002/1873-3468.14718.Peer-Reviewed Original ResearchRediscovering the intricacies of secretory granule biogenesis
Campelo F, Tian M, von Blume J. Rediscovering the intricacies of secretory granule biogenesis. Current Opinion In Cell Biology 2023, 85: 102231. PMID: 37657367, DOI: 10.1016/j.ceb.2023.102231.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSG biogenesisRegulated secretionImmature SGsEssential cellular processesSecretory granulesSecretory granule biogenesisImmature secretory granulesCargo selectionCargo receptorsCellular processesGranule biogenesisGranule cargoCargo moleculesBiogenesisBiophysical mechanismsDisease mechanismsCellular communicationCargoCentral roleDiverse rangeNew lightTraffickingMechanismProteinLipid Sorting and Organelle Identity.
Kim Y, Burd C. Lipid Sorting and Organelle Identity. Cold Spring Harbor Perspectives In Biology 2023, 15: a041397. PMID: 37487627, PMCID: PMC10547387, DOI: 10.1101/cshperspect.a041397.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsTrafficking of lipidsSynthesis of sphingolipidsLipid transfer proteinOrganelle identityMembrane traffickingOrganelle homeostasisSoluble lipid transfer proteinsBulk membrane propertiesOrganelle functionGolgi networkLipid sortingNet membrane chargeOrganelle membranesPlasma membraneEndoplasmic reticulumParticular lipidsOrganellesTransfer proteinLeaflet asymmetryEssential roleBulk membraneCholesterol fluxTraffickingMembraneSecretoryGrowing thin — How bulk lipid transport drives expansion of the autophagosome membrane but not of its lumen
Melia T. Growing thin — How bulk lipid transport drives expansion of the autophagosome membrane but not of its lumen. Current Opinion In Cell Biology 2023, 83: 102190. PMID: 37385155, PMCID: PMC10528516, DOI: 10.1016/j.ceb.2023.102190.Peer-Reviewed Original ResearchAlternative glucose uptake mediated by β-catenin/RSK1 axis under stress stimuli in mammalian cells
Wang C, Lin R, Qi X, Xu Q, Sun X, Zhao Y, Jiang T, Jiang J, Sun Y, Deng Y, Wen J. Alternative glucose uptake mediated by β-catenin/RSK1 axis under stress stimuli in mammalian cells. Biochemical Pharmacology 2023, 214: 115645. PMID: 37321415, DOI: 10.1016/j.bcp.2023.115645.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsbeta CateninBiological TransportGlucoseGlycogen Synthase Kinase 3 betaGTPase-Activating ProteinsMammalsPhosphorylationConceptsPhosphorylation of TBC1D4Glucose uptakeGlucose transportActivation of RSK1Stress conditionsMechanism of glucose uptakeTranslocation of GLUT4Translocation of glucose transportersGlucose transporter translocationStress stimuliIncreased glucose uptakeB-cateninGlucose starvationEfficiency of glucose uptakeProtein complexesMammalian cellsScaffold proteinStress signalsCellular energy utilizationFamily 4TBC1D4Kinase activityRSK1Cellular adaptationCellular membranesDirect determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer
Panda A, Giska F, Duncan A, Welch A, Brown C, McAllister R, Hariharan P, Goder J, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman J, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nature Methods 2023, 20: 891-897. PMID: 37106230, PMCID: PMC10932606, DOI: 10.1038/s41592-023-01864-5.Peer-Reviewed Original ResearchConceptsIntegral membrane proteinsMembrane proteinsOligomeric organizationOligomeric stateNative mass spectrometry analysisFunctional oligomeric stateKey membrane componentMass spectrometry analysisNMS analysisTarget membraneLipid bindingMembrane componentsProteolipid vesiclesMembrane compositionLipid compositionSpectrometry analysisLipid membranesNeurotransmitter releaseProteinMembraneLipidsMembrane propertiesDirect determinationBilayersTransportersA macrocyclic peptide inhibitor traps MRP1 in a catalytically incompetent conformation
Pietz H, Abbas A, Johnson Z, Oldham M, Suga H, Chen J. A macrocyclic peptide inhibitor traps MRP1 in a catalytically incompetent conformation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2220012120. PMID: 36893260, PMCID: PMC10089224, DOI: 10.1073/pnas.2220012120.Peer-Reviewed Original ResearchConceptsMultidrug resistance protein 1MRP1 overexpressionSubstrate transportAdenosine triphosphate-binding cassetteCatalytically incompetent conformationStructurally unrelated moleculesCpI1Small molecule inhibitorsAdenosine triphosphatePlasma membraneA-resolutionCryoelectron microscopyCryo-EMUnrelated moleculesConformational changesMultidrug resistanceMRP1 functionProtein 1Macrocyclic peptidesMultidrug transporter P-glycoproteinFlexible sidechainsXenobiotic compoundsNanomolar potencyCellular toxicityTherapeutic candidate
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply