2024
A fluorescence-based assay for measuring polyamine biosynthesis aminopropyl transferase–mediated catalysis
Singh P, Choi J, Wang W, Lam T, Lechner P, Vanderwal C, Pou S, Nilsen A, Mamoun C. A fluorescence-based assay for measuring polyamine biosynthesis aminopropyl transferase–mediated catalysis. Journal Of Biological Chemistry 2024, 300: 107832. PMID: 39342998, PMCID: PMC11541840, DOI: 10.1016/j.jbc.2024.107832.Peer-Reviewed Original ResearchAminopropyl transferaseFluorescence-based assayLack of high-throughput assaysHigh-throughput screeningCarbon chain lengthChemical librariesMass spectrometryChain lengthHigh-throughput assayDrug discoveryMass spectrometry analysisSaccharomyces cerevisiaeThin-layer chromatographyFluorescence intensityCellular functionsSpectrometry analysisPolycationic moleculesFluorescent conjugatesIsoindoleAPT activityCatalysisAssayBenzeneAdductsEnzyme
2023
Babesia BdFE1 esterase is required for the anti-parasitic activity of the ACE inhibitor fosinopril
Vydyam P, Choi J, Gihaz S, Chand M, Gewirtz M, Thekkiniath J, Lonardi S, Gennaro J, Mamoun C. Babesia BdFE1 esterase is required for the anti-parasitic activity of the ACE inhibitor fosinopril. Journal Of Biological Chemistry 2023, 299: 105313. PMID: 37797695, PMCID: PMC10663679, DOI: 10.1016/j.jbc.2023.105313.Peer-Reviewed Original ResearchConceptsAngiotensin converting enzyme (ACE) inhibitorsACE inhibitor fosinoprilTick-borne illnessConverting Enzyme InhibitorsVector-borne parasitic diseaseClass of drugsNovel drug targetsApicomplexan parasitesMass spectrometry analysisAnti-parasitic activityHeart failureSafe therapyParasite developmentDrug targetsEnzyme inhibitorsParasitic diseasesDrug resistanceTreatment of diseasesHuman babesiosisBabesia parasitesIntraerythrocytic parasitesSuch diseasesDiseaseSpectrometry analysisParasitesFalse-Positive Glycopeptide Identification via In-FAIMS Fragmentation
Rangel-Angarita V, Mahoney K, Kwon C, Sarker R, Lucas T, Malaker S. False-Positive Glycopeptide Identification via In-FAIMS Fragmentation. JACS Au 2023, 3: 2498-2509. PMID: 37772174, PMCID: PMC10523363, DOI: 10.1021/jacsau.3c00264.Peer-Reviewed Original ResearchDirect determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer
Panda A, Giska F, Duncan A, Welch A, Brown C, McAllister R, Hariharan P, Goder J, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman J, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nature Methods 2023, 20: 891-897. PMID: 37106230, PMCID: PMC10932606, DOI: 10.1038/s41592-023-01864-5.Peer-Reviewed Original ResearchConceptsIntegral membrane proteinsMembrane proteinsOligomeric organizationOligomeric stateNative mass spectrometry analysisFunctional oligomeric stateKey membrane componentMass spectrometry analysisNMS analysisTarget membraneLipid bindingMembrane componentsProteolipid vesiclesMembrane compositionLipid compositionSpectrometry analysisLipid membranesNeurotransmitter releaseProteinMembraneLipidsMembrane propertiesDirect determinationBilayersTransporters
2022
Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation
Hong Z, Adlakha J, Wan N, Guinn E, Giska F, Gupta K, Melia TJ, Reinisch KM. Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation. Journal Of Cell Biology 2022, 221: e202207022. PMID: 36282247, PMCID: PMC9597353, DOI: 10.1083/jcb.202207022.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumLipid dropletsProtein-mediated transferLipid transport proteinsLipid droplet formationLD biologyMitochondrial proteinsSecretory pathwayMass spectrometry analysisTerminal domainMitochondrial morphologyTransport proteinsLipid transportersCellular membranesLD metabolismMembrane contactX-ray structureSpectrometry analysisOrganellesGlycerophospholipidsProteinHydrophobic cavityFatty acidsLipidsMembrane
2021
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis.
Sirois I, Isabelle M, Duquette J, Saab F, Caron E. Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis. Journal Of Visualized Experiments 2021 PMID: 34723952, DOI: 10.3791/63052.Peer-Reviewed Original ResearchConceptsImmunopeptidomics workflowsComposition of peptidesSample preparation procedureQuality control samplesMass spectrometry analysisPreparation procedureElution efficiencySample preparationSpectrometry analysisPeptidesImmunoaffinity purificationReagentsMoleculesComplexesPreparationPurificationStepMHC-peptide complexesControl samplesImmunopeptidomicsNew protocolReproducibilityEfficiencyBeadsTechnology platform
2020
Differential Protein Expression in Striatal D1- and D2-Dopamine Receptor-Expressing Medium Spiny Neurons
Mansuri MS, Peng G, Wilson RS, Lam TT, Zhao H, Williams KR, Nairn AC. Differential Protein Expression in Striatal D1- and D2-Dopamine Receptor-Expressing Medium Spiny Neurons. Proteomes 2020, 8: 27. PMID: 33066078, PMCID: PMC7709116, DOI: 10.3390/proteomes8040027.Peer-Reviewed Original ResearchFluorescence-activated nuclear sortingTranslating Ribosome Affinity PurificationSpecific neuronal cell typesBiological replicatesNeuronal cell typesCell typesRibosome affinity purificationDifferential protein expressionSpecific cell typesMice/Major cell typesNuclear sortingMass spectrometry analysisProteome analysisAffinity purificationNeuronal cell populationsMedium spiny neuronsProteinProteomeProtein expressionSpectrometry analysisCell populationsAdaptive changesNetwork analysisReproducible workflowsDimeric Stilbene Antibiotics Target the Bacterial Cell Wall in Drug-Resistant Gram-Positive Pathogens
Goddard TN, Patel J, Park HB, Crawford JM. Dimeric Stilbene Antibiotics Target the Bacterial Cell Wall in Drug-Resistant Gram-Positive Pathogens. Biochemistry 2020, 59: 1966-1971. PMID: 32410442, PMCID: PMC10578317, DOI: 10.1021/acs.biochem.0c00213.Peer-Reviewed Original ResearchConceptsChemical complementation studiesBacterial cell wallMass spectrometry analysisLipid IILead compoundsMultidrug-resistant Gram-positive pathogensStilbene dimersCell wall precursorsSpectrometry analysisDrug-Resistant GramNew antimicrobial agentsGram-positive pathogensDimersNonlethal exposureAntibiotic targetsPositive pathogensDietary plantsAction studiesAntibiotic resistanceMode of actionAntimicrobial agentsLead dimerWall precursorsMonomersStrong activityAntibacterial activity and synergism of the essential oil of Nectandra megapotamica (L.) flowers against OXA-23-producing Acinetobacter baumannii
Vasconcelos N, Mallmann V, Costa É, Simionatto E, Coutinho E, Da Silva R, Ribeiro S, Franco O, Migliolo L, Croda J, Simionatto S. Antibacterial activity and synergism of the essential oil of Nectandra megapotamica (L.) flowers against OXA-23-producing Acinetobacter baumannii. Journal Of Essential Oil Research 2020, 32: 260-268. DOI: 10.1080/10412905.2020.1740802.Peer-Reviewed Original ResearchGas chromatography/mass spectrometry analysisChromatography/mass spectrometry analysisGas chromatography/flame ionization detectorNatural plant essential oilsDrug delivery systemsAntibacterial activityEssential oilMass spectrometry analysisFlame ionization detectorSpectrometry analysisChemical compositionDelivery systemAntimicrobial activityIonization detectorBiological propertiesSynergistic effectOxygenated sesquiterpenesCaryophyllene oxideNew drug developmentOilPlant essential oilsDrug developmentExcipientsOxide
2019
Development of Targeted Mass Spectrometry-Based Approaches for Quantitation of Proteins Enriched in the Postsynaptic Density (PSD)
Wilson RS, Rauniyar N, Sakaue F, Lam TT, Williams KR, Nairn AC. Development of Targeted Mass Spectrometry-Based Approaches for Quantitation of Proteins Enriched in the Postsynaptic Density (PSD). Proteomes 2019, 7: 12. PMID: 30986977, PMCID: PMC6630806, DOI: 10.3390/proteomes7020012.Peer-Reviewed Original ResearchParallel reaction monitoringPostsynaptic densityData-independent acquisition (DIA) approachElectron-dense regionsQuantitation of proteinsBiochemical fractionationMass spectrometry analysisMass spectrometry-based assayProtein abundanceExcitatory glutamatergic synapsesPSD compositionProtein compositionPSD proteinsInternal peptide standardsPSD fractionProteinTargeted Mass SpectrometrySpectrometry analysisGlutamatergic synapsesMass spectrometryPeptide standardsAbundanceNeuropsychiatric disordersCortical brain tissueWide variety
2017
The antimalarial activity of the pantothenamide α-PanAm is via inhibition of pantothenate phosphorylation
Chiu JE, Thekkiniath J, Choi JY, Perrin BA, Lawres L, Plummer M, Virji AZ, Abraham A, Toh JY, Zandt MV, Aly ASI, Voelker DR, Mamoun CB. The antimalarial activity of the pantothenamide α-PanAm is via inhibition of pantothenate phosphorylation. Scientific Reports 2017, 7: 14234. PMID: 29079738, PMCID: PMC5660193, DOI: 10.1038/s41598-017-14074-9.Peer-Reviewed Original ResearchConceptsMode of actionBlood stage developmentPantothenate kinase activityYeast mutantsMass spectrometry analysisBlood-stage parasitesPA-dependent mannerKinase activityPantothenate phosphorylationCAB1Pantothenate kinaseStage parasitesYeast growthCoenzyme ABiochemical analysisBlood stagesDirect competitionPantothenic acidSpectrometry analysisResistant P. falciparum strainsPhosphorylationStage developmentPlasmodium falciparumCompetitive inhibitorP. bergheiNon-invasive assessment of hepatic mitochondrial metabolism by positional isotopomer NMR tracer analysis (PINTA)
Perry RJ, Peng L, Cline GW, Butrico GM, Wang Y, Zhang XM, Rothman DL, Petersen KF, Shulman GI. Non-invasive assessment of hepatic mitochondrial metabolism by positional isotopomer NMR tracer analysis (PINTA). Nature Communications 2017, 8: 798. PMID: 28986525, PMCID: PMC5630596, DOI: 10.1038/s41467-017-01143-w.Peer-Reviewed Original ResearchConceptsMitochondrial metabolismHepatic mitochondrial metabolismPyruvate carboxylase fluxCitrate synthase fluxPyruvate cyclingMitochondrial uncouplerIntermediary metabolismSpectrometry analysisPhysiological conditionsChromatography-mass spectrometry analysisSynthase fluxCentral roleMetabolismHepatic mitochondriaGas chromatography-mass spectrometry analysisVivo NMR spectroscopyMitochondriaNMR spectroscopyRegulationUncouplerRoleTracer analysisVivoMaintenance of normoglycemiaWide range
2015
Comprehensive bioimaging with fluorinated nanoparticles using breathable liquids
Kurczy ME, Zhu ZJ, Ivanisevic J, Schuyler AM, Lalwani K, Santidrian AF, David JM, Giddabasappa A, Roberts AJ, Olivos HJ, O’Brien P, Franco L, Fields MW, Paris LP, Friedlander M, Johnson CH, Epstein AA, Gendelman HE, Wood MR, Felding BH, Patti GJ, Spilker ME, Siuzdak G. Comprehensive bioimaging with fluorinated nanoparticles using breathable liquids. Nature Communications 2015, 6: 5998. PMID: 25601659, PMCID: PMC4454288, DOI: 10.1038/ncomms6998.Peer-Reviewed Original ResearchConceptsF-AuNPsNon-polar moleculesTeflon-like coatingDesorption/ionizationFluorinated nanoparticlesGold nanoparticlesMass spectrometry imagingAnalyte desorptionRemarkable biocompatibilityMass spectrometry analysisElectron microscopyUnique propertiesMass spectrometryMultimodal imagingNanoparticlesContrast agentsSpectrometry imagingSpectrometry analysisFluorocarbonSynthetic bloodFluorocarbon liquidAuNPsLiquidNanostructuresBiocompatibility
2014
Autonomous Metabolomics for Rapid Metabolite Identification in Global Profiling
Benton HP, Ivanisevic J, Mahieu NG, Kurczy ME, Johnson CH, Franco L, Rinehart D, Valentine E, Gowda H, Ubhi BK, Tautenhahn R, Gieschen A, Fields MW, Patti GJ, Siuzdak G. Autonomous Metabolomics for Rapid Metabolite Identification in Global Profiling. Analytical Chemistry 2014, 87: 884-891. PMID: 25496351, PMCID: PMC4303330, DOI: 10.1021/ac5025649.Peer-Reviewed Original ResearchConceptsMass spectrometry data acquisitionSystems biology levelMass spectrometry analysisBioinformatics resourcesGlobal profilingTandem mass spectrometry dataProfiling datasetsMass spectrometry databaseMass spectrometry dataSpectrometry analysisProfilingData acquisitionSpectrometry dataRapid metabolite identificationMetabolomic profilingUntargeted metabolomicsBacterial samplesMetabolomicsSimultaneous data processingIdentificationMetabolite identificationMetabolomics workflowsFliesAutomatic searchAutonomous approach
2013
The lipoprotein La7 contributes to Borrelia burgdorferi persistence in ticks and their transmission to naïve hosts
Yang X, Hegde S, Shroder DY, Smith AA, Promnares K, Neelakanta G, Anderson JF, Fikrig E, Pal U. The lipoprotein La7 contributes to Borrelia burgdorferi persistence in ticks and their transmission to naïve hosts. Microbes And Infection 2013, 15: 729-737. PMID: 23774694, PMCID: PMC3769513, DOI: 10.1016/j.micinf.2013.06.001.Peer-Reviewed Original ResearchConceptsComplex enzootic cycleInner membrane proteinB. burgdorferi persistenceProtein-protein interactionsOuter membrane lipoproteinNaïve hostsSpirochete life cycleCo-immunoprecipitation studiesMammalian infectivityMembrane proteinsRedundant rolesMolecular detailsMammalian hostsMembrane lipoproteinGene expressionWild typeInfection cycleLiquid chromatography-mass spectrometry analysisPathogen survivalBiological significanceHost transmissionEnzootic cyclePathogen transmissionSpectrometry analysisLA7
2012
Enrichment strategies for phosphoproteomics: state-of-the-art
Salovska B, Tichy A, Rezacova M, Vavrova J, Novotna E. Enrichment strategies for phosphoproteomics: state-of-the-art. Reviews In Analytical Chemistry 2012, 31: 29-41. DOI: 10.1515/revac-2011-0025.Peer-Reviewed Original ResearchProtein phosphorylationReversible post-translational modificationPost-translational modificationsAffinity enrichment techniquesPhosphorylated peptides/proteinsSubsequent mass spectrometry analysisAnalysis of phosphorylationEnrichment strategyDifferent enrichment strategiesTranslational regulationPhosphorylation sitesCellular signalingPhosphorylated proteinsMass spectrometry analysisPeptides/proteinsKey regulatorBiological processesChemical derivatizationLow abundancePhosphorylationMass spectrometryProteinSpectrometry analysisNonphosphorylated proteinsEnrichment technique
2010
Deletion of Immunoproteasome Subunits Imprints on the Transcriptome and Has a Broad Impact on Peptides Presented by Major Histocompatibility Complex I molecules*
de Verteuil D, Muratore-Schroeder T, Granados D, Fortier M, Hardy M, Bramoullé A, Caron É, Vincent K, Mader S, Lemieux S, Thibault P, Perreault C. Deletion of Immunoproteasome Subunits Imprints on the Transcriptome and Has a Broad Impact on Peptides Presented by Major Histocompatibility Complex I molecules*. Molecular & Cellular Proteomics 2010, 9: 2034-2047. PMID: 20484733, PMCID: PMC2938112, DOI: 10.1074/mcp.m900566-mcp200.Peer-Reviewed Original ResearchConceptsMIP repertoireLabel-free quantitative proteomics approachComplex ICell type-specific signaturesBasic cellular processesUnstructured protein regionsProteasome-mediated proteolysisQuantitative proteomics approachSet of genesPrimary mouse dendritic cellsJawed vertebratesRegulated genesCellular processesProteomic approachMass spectrometry analysisGene transcriptionBioinformatics analysisChromosome 4Protein regionsPrimary cellsGenesCleavage preferenceSpectrometry analysisCell functionPeptidomics studies
2009
Evaluating the Intrinsic Cysteine Redox-Dependent States of the A-Chain of Human Insulin Using NMR Spectroscopy, Quantum Chemical Calculations, and Mass Spectrometry
Sharma AK, Ling Y, Greer AB, Hafler DA, Kent SC, Zhang Y, Rigby AC. Evaluating the Intrinsic Cysteine Redox-Dependent States of the A-Chain of Human Insulin Using NMR Spectroscopy, Quantum Chemical Calculations, and Mass Spectrometry. The Journal Of Physical Chemistry B 2009, 114: 585-591. PMID: 19954153, PMCID: PMC2829747, DOI: 10.1021/jp908729h.Peer-Reviewed Original ResearchConceptsQuantum chemical calculationsChemical calculationsFree thiol moietyNMR spectroscopy dataA-chain peptideRedox chemistryNMR spectroscopyThiol moietyCell surface class II moleculesMass spectrometry analysisOxidized stateFunctional studiesA-chain analogueIntrinsic cysteine residuesMass spectrometryPeptide interactionsConformational equilibriumSpectroscopy dataRedox-dependent mechanismDisulfide conformationSpectrometry analysisDependent conformational equilibriumPrevious functional studiesConformationProtein systems
2008
Retroviruses Human Immunodeficiency Virus and Murine Leukemia Virus Are Enriched in Phosphoinositides
Chan R, Uchil PD, Jin J, Shui G, Ott DE, Mothes W, Wenk MR. Retroviruses Human Immunodeficiency Virus and Murine Leukemia Virus Are Enriched in Phosphoinositides. Journal Of Virology 2008, 82: 11228-11238. PMID: 18799574, PMCID: PMC2573248, DOI: 10.1128/jvi.00981-08.Peer-Reviewed Original ResearchConceptsPlasma membraneMurine leukemia virusHIV-1 buddingLipid contentLeukemia virusMass spectrometry analysisCholesterol-rich regionsOverall lipid contentCell peripheryMutant virionsLipid envelopeTotal lipid contentPhosphorylated derivativesSpectrometry analysisPhosphoinositideRetrovirusesMembraneCellsMatrix domainParticle releaseHuman immunodeficiency virus type 1Immunodeficiency virus type 1VirusVirus type 1Phosphatidylinositol
2002
Automation of Nanoscale Microcapillary Liquid Chromatography−Tandem Mass Spectrometry with a Vented Column
Licklider L, Thoreen C, Peng J, Gygi S. Automation of Nanoscale Microcapillary Liquid Chromatography−Tandem Mass Spectrometry with a Vented Column. Analytical Chemistry 2002, 74: 3076-3083. PMID: 12141667, DOI: 10.1021/ac025529o.Peer-Reviewed Original ResearchConceptsPeptide mixturesSample introduction stepComplex peptide mixturesTandem MS experimentsMS analysisReversed phase LC-MS/MS analysisLiquid chromatography-tandem mass spectrometry analysisColumn enrichmentPeptide samplesElectrospray sourceMass spectrometry analysisMS experimentsCapillary columnLarge sample volumesMass spectrometryLC-MS/MS analysisColumn washingIntroduction stepChromatography fractionsLine separationSpectrometry analysisNano-LCLoop injectorSample volumeColumn
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