2022
Co-Expression and Functional Interactions of Death Receptor 3 and E-Selectin in Clear Cell Renal Cell Carcinoma
Al-Lamki RS, Wang J, Pober JS, Bradley JR. Co-Expression and Functional Interactions of Death Receptor 3 and E-Selectin in Clear Cell Renal Cell Carcinoma. American Journal Of Pathology 2022, 192: 722-736. PMID: 35063404, DOI: 10.1016/j.ajpath.2021.12.010.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinaseCell cycle entryCcRCC cellsDeath receptor 3Protein kinaseClear cell renal cell carcinoma cellsProximity ligation assayRenal cell carcinoma cellsReceptor 3E-selectinPotential new targetsCycle entryNF-κB-dependent mannerNF-κBLigation assayTumor gradeCcRCC tissuesFunctional interactionSelectin expressionFunctional roleClear cell renal cell carcinomaOrgan cultureCell renal cell carcinomaExpression increasesAddition of TL1A
2017
Nitrogen mustard-induced corneal injury involves the sphingomyelin-ceramide pathway
Charkoftaki G, Jester JV, Thompson DC, Vasiliou V. Nitrogen mustard-induced corneal injury involves the sphingomyelin-ceramide pathway. The Ocular Surface 2017, 16: 154-162. PMID: 29129753, PMCID: PMC7376578, DOI: 10.1016/j.jtos.2017.11.004.Peer-Reviewed Original ResearchConceptsCorneal damageNM exposureSphingomyelin-ceramide pathwayCorneal stromaIrreversible corneal damageNitrogen mustardAltered lipid profileSulfur mustardCorneal injuryLipid profileCentral corneaCorneal epitheliumRabbit eyesSpecific sphingomyelinsPotent vesicantCorneaOrgan cultureStromaLipidomic analysisExposureMorphological changesDamaging effectsDamageInjuryPathway
2016
In vitro functional screening as a means to identify new plasticizers devoid of reproductive toxicity
Boisvert A, Jones S, Issop L, Erythropel HC, Papadopoulos V, Culty M. In vitro functional screening as a means to identify new plasticizers devoid of reproductive toxicity. Environmental Research 2016, 150: 496-512. PMID: 27423704, DOI: 10.1016/j.envres.2016.06.033.Peer-Reviewed Original ResearchConceptsOrgan cultureDose-response studySensitive developmental windowsFetal Leydig cellsGerm cellsAndrogen productionTesticular functionBiphasic effectSteroid productionReproductive toxicityLeydig cellsSpermatogonial cell lineMonoester metabolitesNeonatal testisSafe alternativeScreening toolDevelopment of compoundsVivo evaluationCell linesMA-10Developmental windowCell line dataCell survivalEndocrine disruptorsPromising compounds
2013
A synthetic PPAR-γ agonist triterpenoid ameliorates experimental fibrosis: PPAR-γ-independent suppression of fibrotic responses
Wei J, Zhu H, Komura K, Lord G, Tomcik M, Wang W, Doniparthi S, Tamaki Z, Hinchcliff M, Distler JH, Varga J. A synthetic PPAR-γ agonist triterpenoid ameliorates experimental fibrosis: PPAR-γ-independent suppression of fibrotic responses. Annals Of The Rheumatic Diseases 2013, 73: 446-454. PMID: 23515440, PMCID: PMC4028127, DOI: 10.1136/annrheumdis-2012-202716.Peer-Reviewed Original ResearchMeSH KeywordsAdipogenesisAdultAnimalsBiopsyCells, CulturedCollagenDisease Models, AnimalDrug Evaluation, PreclinicalFemaleFibroblastsFibrosisHumansInfant, NewbornMiceMice, Inbred C57BLOleanolic AcidOrgan Culture TechniquesPPAR gammaProto-Oncogene Proteins c-aktScleroderma, SystemicSignal TransductionSkinTransforming Growth Factor betaConceptsSkin organ cultureHuman skin organ cultureAntifibrotic effectsDermal fibrosisExperimental fibrosisOrgan culturePeroxisome proliferator-activated receptor γModulation of fibrogenesisProliferator-activated receptor γHuman skin equivalentsPotential new therapiesPotential therapeutic strategyFibrotic gene expressionSynthetic oleanane triterpenoidComplementary mouse modelsControl of fibrosisPersistent fibroblast activationGrowth factor βTGF-β signalingEffects of CDDOSystemic sclerosisBleomycin injectionFibrogenic responseFibrotic activityMurine model
2009
TNF Receptors Differentially Signal and Are Differentially Expressed and Regulated in the Human Heart
Al-Lamki R, Brookes AP, Wang J, Reid MJ, Parameshwar J, Goddard MJ, Tellides G, Wan T, Min W, Pober JS, Bradley JR. TNF Receptors Differentially Signal and Are Differentially Expressed and Regulated in the Human Heart. American Journal Of Transplantation 2009, 9: 2679-2696. PMID: 19788501, PMCID: PMC3517885, DOI: 10.1111/j.1600-6143.2009.02831.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell CycleCell DeathEndothelium, VascularEnzyme ActivationGraft RejectionHeart TransplantationHumansMAP Kinase Kinase Kinase 5MiceMice, KnockoutMyocardiumMyocytes, CardiacOrgan Culture TechniquesProtein-Tyrosine KinasesReceptors, Tumor Necrosis Factor, Type IReceptors, Tumor Necrosis Factor, Type IIRNA, MessengerTumor Necrosis Factor-alphaConceptsVascular endothelial cellsCardiac allograftsCell cycle entryApoptosis signal-regulating kinase 1Cycle entryExpression of TNFTNFR1 knockoutNecrosis factorTarget cell responseTNFTNF receptorCardiac fibroblastsCell responsesSignal-regulated kinases 1TNF responseASK1 activationMyocardiumEndothelial cellsEpithelial tyrosine kinaseTNFR2Human heartOrgan cultureTNFR1Etk activationAllografts
2006
Attenuation of signaling pathways stimulated by pathologically activated FGF-receptor 2 mutants prevents craniosynostosis
Eswarakumar VP, Özcan F, Lew ED, Bae JH, Tomé F, Booth CJ, Adams DJ, Lax I, Schlessinger J. Attenuation of signaling pathways stimulated by pathologically activated FGF-receptor 2 mutants prevents craniosynostosis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 18603-18608. PMID: 17132737, PMCID: PMC1693709, DOI: 10.1073/pnas.0609157103.Peer-Reviewed Original ResearchConceptsFGF receptorsDocking protein FRS2alphaCommon craniofacial abnormalitySelective uncouplingPremature fusionSkull developmentFunction mutationsDominant mutationsNormal skull developmentMurine model systemMutationsSevere bone disordersModel systemFGFR2cCraniofacial abnormalitiesCalvaria organ culturesPathwayOrgan cultureFRS2alphaSkull vaultMutantsPharmacological approachesFusionFGFRUncoupling
1996
Colony stimulating factor‐1 plays a role in osteoclast formation and function in bone resorption induced by parathyroid hormone and parathyroid hormone‐related protein
Weir E, Lowik C, Paliwal I, Insogna K. Colony stimulating factor‐1 plays a role in osteoclast formation and function in bone resorption induced by parathyroid hormone and parathyroid hormone‐related protein. Journal Of Bone And Mineral Research 1996, 11: 1474-1481. PMID: 8889847, DOI: 10.1002/jbmr.5650111014.Peer-Reviewed Original ResearchConceptsBone resorptionMature osteoclastsParathyroid hormoneOsteoclast precursorsFetal rat long bonesParathyroid hormone-related proteinResistant acid phosphatase-positive osteoclastsMitogenic activityFactor 1Hormone-related proteinOrgan cultureCSF-1Rat long bonesTreatment of boneSignificant increaseOrgan culture assayHistological examinationBone assayOsteoclast recruitmentOsteoclast formationRat osteoclastsColony stimulating factor 1CSF-1 productionPTHResorption
1995
EAR BIOPSY LOCATION INFLUENCES DETECTION OF BORRELIA BURGDORFERI BY PCR, BUT NOT BY CULTURE IN NATURALLY INFECTED PEROMYSCUS LEUCOPUS
Hofmeister E, Childs J. EAR BIOPSY LOCATION INFLUENCES DETECTION OF BORRELIA BURGDORFERI BY PCR, BUT NOT BY CULTURE IN NATURALLY INFECTED PEROMYSCUS LEUCOPUS. Journal Of Wildlife Diseases 1995, 31: 345-51. PMID: 8592355, DOI: 10.7589/0090-3558-31.3.345.Peer-Reviewed Original ResearchConceptsEar biopsy samplesPolymerase chain reactionBiopsy samplesBiopsy locationB. burgdorferiBorrelia burgdorferiInfected micePeripheral samplesIndividual miceInfection statusMore tissue samplesMiceTissue samplesHigh concordanceChain reactionOrgan cultureBurgdorferiGreater frequencyEar samplesWhite-footed miceEnzootic areaEarCentral sample
1992
Chronic fluoxetine treatment reduces hypothalamic vasopressin secretion in vitro
Altemus M, Cizza G, Gold P. Chronic fluoxetine treatment reduces hypothalamic vasopressin secretion in vitro. Brain Research 1992, 593: 311-313. PMID: 1450938, DOI: 10.1016/0006-8993(92)91326-a.Peer-Reviewed Original Research
1991
Expression of endothelial-leukocyte adhesion molecule-1 in elicited late phase allergic reactions.
Leung DY, Pober JS, Cotran RS. Expression of endothelial-leukocyte adhesion molecule-1 in elicited late phase allergic reactions. Journal Of Clinical Investigation 1991, 87: 1805-1809. PMID: 1708785, PMCID: PMC295299, DOI: 10.1172/jci115201.Peer-Reviewed Original ResearchConceptsLate-phase reactionInflammatory cellsAllergic reactionsEC activationLate-phase allergic reactionsEndothelial leukocyte adhesion molecule-1Adhesion moleculesIntradermal allergen injectionSequential skin biopsiesInflammatory cell infiltrateNeutrophil adhesion moleculesEndothelial leukocyte adhesion moleculeAllergic skin reactionsELAM-1 expressionOrgan cultureAdhesion molecule-1Skin organ cultureAtopic patientsCell infiltrateEndothelial activationAllergen injectionRespiratory allergySkin reactionsTNF-alphaIntradermal injection
1988
Effects of recombinant gamma-interferon on HLA-DR and DQ expression by skin cells in short-term organ culture.
Messadi DV, Pober JS, Murphy GF. Effects of recombinant gamma-interferon on HLA-DR and DQ expression by skin cells in short-term organ culture. Laboratory Investigation 1988, 58: 61-7. PMID: 3121914.Peer-Reviewed Original ResearchConceptsShort-term organ cultureHLA-DRLangerhans cellsOrgan cultureT6-positive Langerhans cellsClass II major histocompatibility antigensImmune interferonClass II major histocompatibility moleculesEpidermal dendritic cellsCutaneous immune responsesDendritic cell populationsHLA-DR expressionSkin microvascular endothelial cellsMajor histocompatibility antigensMicrovascular endothelial cellsSkin cellsNewborn human foreskinMajor histocompatibility moleculesDendritic cellsInflammatory mediatorsDQ expressionHLA-DQOrgan culture systemHistocompatibility antigensImmune response
1987
Induction of an activation antigen on postcapillary venular endothelium in human skin organ culture.
Messadi DV, Pober JS, Fiers W, Gimbrone MA, Murphy GF. Induction of an activation antigen on postcapillary venular endothelium in human skin organ culture. The Journal Of Immunology 1987, 139: 1557-62. PMID: 3497975, DOI: 10.4049/jimmunol.139.5.1557.Peer-Reviewed Original ResearchConceptsTumor necrosis factorActivation antigensNecrosis factorInterleukin-1Microvascular endotheliumEndothelial cellsEndothelial activation antigenMediators interleukin-1Postcapillary venular endotheliumCultured human umbilical vein endothelial cellsSuperficial vascular plexusOrgan cultureHuman skin organ cultureShort-term organ cultureHuman umbilical vein endothelial cellsSkin organ cultureUmbilical vein endothelial cellsVenular endothelial cellsMicrovascular stainingPostcapillary venular endothelial cellsVein endothelial cellsEndogenous cytokinesLymphocyte traffickingInterleukin-2Venular endotheliumAortic endothelial cell proteoheparan sulfate. II. Modulation by extracellular matrix.
Keller R, Pratt B, Furthmayr H, Madri J. Aortic endothelial cell proteoheparan sulfate. II. Modulation by extracellular matrix. American Journal Of Pathology 1987, 128: 299-306. PMID: 2956886, PMCID: PMC1899612.Peer-Reviewed Original ResearchConceptsExtracellular matrixPlasma membraneHS IProteoheparan sulfate speciesSulfate biosynthesisEndothelial cellsHS IIIHS IICell polarityMajor cell typesExtracellular matrix componentsPolarized secretionBovine aortic endothelial cellsOrgan cultureTissue culture plasticSubcellular matrixBiosynthetic phenotypeMedium of cellsDifferentiated phenotypeCell typesMatrix moleculesVascular endothelial cellsAortic endothelial cellsProteoheparan sulfateSpecies
1983
Cytotoxicity of a Perfluorocarbon Blood Substitute to Macrophages in Vitro
Bucala R, Kawakami M, Cerami A. Cytotoxicity of a Perfluorocarbon Blood Substitute to Macrophages in Vitro. Science 1983, 220: 965-967. PMID: 6844922, DOI: 10.1126/science.6844922.Peer-Reviewed Original Research
1982
Fetal lung in organ culture. IV. Supra-additive hormone interactions
Gross I, Wilson C. Fetal lung in organ culture. IV. Supra-additive hormone interactions. Journal Of Applied Physiology 1982, 52: 1420-1425. PMID: 6286561, DOI: 10.1152/jappl.1982.52.6.1420.Peer-Reviewed Original ResearchConceptsFetal lung maturationLung maturationSupra-additive interactionOrgan cultureCombination of dexamethasoneRate of incorporationFetal rat lungVivo animal studiesHormone administrationFetal lungAnimal studiesRat lungSupra-additive stimulationThyroid hormonesNM dexamethasoneDexamethasone concentrationsMaximal stimulationLungDexamethasoneNM triiodothyroninePulmonary surfactantSignificant increaseStimulationPrecursor incorporationCyclic monophosphate
1981
Partial purification and characterization of a nerve trophic factor regulating muscle acetylcholinesterase activity
Lentz T, Addis J, Chester J. Partial purification and characterization of a nerve trophic factor regulating muscle acetylcholinesterase activity. Experimental Neurology 1981, 73: 542-557. PMID: 7262254, DOI: 10.1016/0014-4886(81)90288-0.Peer-Reviewed Original ResearchConceptsAcetylcholinesterase activityOrgan cultureRat brain homogenatesMuscle acetylcholinesterase activityTrophic factorsBrain homogenatesUntreated musclesHigh acetylcholinesterase activityTriceps muscleTrophic effectsMuscleUntreated controlsBasic proteinMuscle acetylcholinesteraseIsoelectric focusingActive factorsActive fractionAcetylcholinesteraseMolecular formsNeurotrophinsActivityFactorsSDS-polyacrylamide gel electrophoresisOrganotypic Culture of Fetal Rat Lung: Evaluation and Comparison with Organ Culture1–3
Gross I, Freedman R, Wilson C, Lindsey S. Organotypic Culture of Fetal Rat Lung: Evaluation and Comparison with Organ Culture1–3. American Journal Of Respiratory And Critical Care Medicine 1981, 123: 313-9. PMID: 7224342, DOI: 10.1164/arrd.1981.123.3.313.Peer-Reviewed Original Research
1980
Insulin antagonism of dexamethasone-induced stimulation of cholinephosphate cytidylyltransferase in fetal rat lung in organ culture
Rooney S, Ingleson L, Wilson C, Gross I. Insulin antagonism of dexamethasone-induced stimulation of cholinephosphate cytidylyltransferase in fetal rat lung in organ culture. Lung 1980, 158: 151-155. PMID: 6255261, DOI: 10.1007/bf02713717.Peer-Reviewed Original ResearchThe Influence of Hormones on the Biochemical Development of Fetal Rat Lung in Organ Culture. II. Insulin
Gross I, Walker Smith G, Wilson C, Maniscalco W, Ingleson L, Brehier A, Rooney S. The Influence of Hormones on the Biochemical Development of Fetal Rat Lung in Organ Culture. II. Insulin. Pediatric Research 1980, 14: 834-838. PMID: 6995921, DOI: 10.1203/00006450-198006000-00012.Peer-Reviewed Original ResearchConceptsFetal rat lungRat lungCholine incorporationSignificant decreasePhospholipid fractionInfluence of insulinEffect of insulinInfluence of hormonesPercentage of radioactivityType II cellsLung cell growthLung cell differentiationLung maturationOrgan culture systemInsulin treatmentMorphologic evidencePhospholipid radioactivityInsulinMorphologic maturationSurfactant phospholipidsGlycogen contentII cellsOrgan cultureBiochemical developmentLamellar bodiesFetal lung in organ culture. III. Comparison of dexamethasone, thyroxine, and methylxanthines
Gross I, Wilson C, Ingleson L, Brehier A, Rooney S. Fetal lung in organ culture. III. Comparison of dexamethasone, thyroxine, and methylxanthines. Journal Of Applied Physiology 1980, 48: 872-877. PMID: 7451297, DOI: 10.1152/jappl.1980.48.5.872.Peer-Reviewed Original ResearchConceptsFetal lungComparison of dexamethasoneLung glycogen contentSurfactant-associated phospholipidsFetal rat lungPercentage of radioactivityRat lungAminophyllineCholine-containing phospholipidsThyroxineGlycogen contentCholine incorporationLungCaffeine treatmentSignificant decreaseOrgan cultureRate of incorporationSignificant increaseLysolecithin acyltransferaseExposure of explantsCholinephosphate cytidylyltransferaseDexamethasoneDifferent effectsMembrane phospholipidsMethylxanthines
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