2025
The wavelength of the ciliary beat in Chlamydomonas saturates at long ciliary lengths
Lee E, Ouyang X, Howard J. The wavelength of the ciliary beat in Chlamydomonas saturates at long ciliary lengths. Biophysical Journal 2025, 124: 2961-2969. PMID: 40770878, DOI: 10.1016/j.bpj.2025.07.037.Peer-Reviewed Original ResearchConceptsCiliary lengthBiflagellate green alga Chlamydomonas reinhardtiiGreen alga Chlamydomonas reinhardtiiAlga Chlamydomonas reinhardtiiShort ciliaMammalian spermChlamydomonas mutantsChlamydomonas reinhardtiiMis-regulationLow ATP concentrationsCiliary beatingSea urchinsLong ciliaWild-typeCiliaChlamydomonasMutantsLength-dependent differencesBiochemical differencesATP concentrationMotilityReinhardtiiSpermFlagellaeSwimming speedGlycogen supports glycolytic plasticity in neurons
Singh M, Wolfe A, Vishwanath A, Tsives A, Gonzalez I, Emerson S, Goodman R, Colón-Ramos D. Glycogen supports glycolytic plasticity in neurons. Proceedings Of The National Academy Of Sciences Of The United States Of America 2025, 122: e2509003122. PMID: 40638090, PMCID: PMC12280961, DOI: 10.1073/pnas.2509003122.Peer-Reviewed Original ResearchConceptsCaenorhabditis elegans</i>Conditions of mitochondrial dysfunctionCells of living animalsSynaptic vesicle recyclingTransient hypoxiaSingle cellsVesicle recyclingRNAi screenAssociated with defectsMitochondrial functionResponse to activationGlycolytic stateMitochondrial dysfunctionEnergy metabolism in vivoMetabolism in vivoSynaptic functionNeuronsIn vivoHypoxiaEnergy reservesGlycogen phosphorylaseGlycogenOrthologsCaenorhabditisMutantsA genetic modifier links integrin α5 to the phenotypic variation in fibronectin 1a mutant zebrafish
Capon S, Maroufidou A, Feltes M, Xu Y, Matharoo D, Jülich D, Holley S, Farber S, Stainier D. A genetic modifier links integrin α5 to the phenotypic variation in fibronectin 1a mutant zebrafish. PLOS Genetics 2025, 21: e1011747. PMID: 40549824, PMCID: PMC12212883, DOI: 10.1371/journal.pgen.1011747.Peer-Reviewed Original ResearchConceptsMutant phenotypePhenotypic variationNonsense mutationGenetic modifiersDouble mutant analysisWhole-genome sequencingITGA5 expressionSevere phenotypeIntegrin alpha 5Mutant analysisMutant larvaeProximal promoterMutantsMutant zebrafishCardia bifidaGene expressionIntegrin A5Genetic backgroundAlpha 5PhenotypeMutationsExpression levelsFibronectinFunctional cardiovascular systemZebrafishIdentification of F-box proteins in ABA- and GA-regulated seed germination: interaction of GASA1 signalling peptide and ABA-induced ubiquitination
Kasera M, Ceciliato P, Lopez B, Hauser F, Gendron J, Schroeder J. Identification of F-box proteins in ABA- and GA-regulated seed germination: interaction of GASA1 signalling peptide and ABA-induced ubiquitination. Philosophical Transactions Of The Royal Society B Biological Sciences 2025, 380: 20240233. PMID: 40439299, PMCID: PMC12121378, DOI: 10.1098/rstb.2024.0233.Peer-Reviewed Original ResearchConceptsSeed germinationSignal peptideGenetic screeningF-box proteinsForward genetic screensHomology-based gene silencingInhibition of seed germinationABA-insensitiveHomologous genesFunctional genomicsGene familyTriple mutantF-boxABA inhibitionProteasome pathwayAmiRNA linesGene silencingInteraction partnersGerminating seedsArtificial microRNAGenesABAGerminationMutantsUbiquitinScreening single nucleotide changes to tropomyosin to identify novel cardiomyopathy mutants
Wen J, Campbell S, Moore J, Lehman W, Rynkiewicz M. Screening single nucleotide changes to tropomyosin to identify novel cardiomyopathy mutants. Journal Of Molecular And Cellular Cardiology 2025, 203: 82-90. PMID: 40268117, PMCID: PMC12135046, DOI: 10.1016/j.yjmcc.2025.04.009.Peer-Reviewed Original ResearchConceptsDeleterious effects of mutationsTropomyosin-actin interactionsSingle nucleotide mutationsEffects of mutationsThin filament regulationDilated CardiomyopathyTropomyosin sequencesActin interactionNucleotide mutationsFilament regulationMutation-sensitiveSubcellular unitsMutantsPathological cardiac remodelingTropomyosinActinThin filamentsEarly therapeutic interventionFunctional analysisMutationsInherited cardiomyopathyCardiac remodelingCardiac functionRegulatory BCardiomyopathyIdentification of a Coxiella burnetii outer membrane porin required for intracellular replication
Yang Z, Duncan-Lowey J, Roy C. Identification of a Coxiella burnetii outer membrane porin required for intracellular replication. Infection And Immunity 2025, 93: e00448-24. PMID: 40071978, PMCID: PMC11977307, DOI: 10.1128/iai.00448-24.Peer-Reviewed Original ResearchConceptsIntracellular replicationDot/Icm type IVB secretion systemType IVB secretion systemHost cellsIsogenic wild-type strainIntracellular replication defectsIVB secretion systemEpitope-tagged proteinsOuter membrane porinsTransposon mutagenesis screenWild-type strainEpitope-tagged versionHost cell functionsBacterial outer membraneSecretion systemMembrane porinsMutagenesis screenOuter membraneReplication defectsHuman Q feverBacterial porinsMutantsGram-negativeIntracellular pathogensPorinBorrelia burgdorferi serine protease HtrA is a pleiotropic regulator of stress response, motility, flagellar hemostasis, and infectivity
Zhang K, Sze C, Zhao H, Liu J, Li C. Borrelia burgdorferi serine protease HtrA is a pleiotropic regulator of stress response, motility, flagellar hemostasis, and infectivity. Communications Biology 2025, 8: 341. PMID: 40025221, PMCID: PMC11873206, DOI: 10.1038/s42003-025-07781-x.Peer-Reviewed Original ResearchConceptsBacterial stress responseStress responseRegulators of bacterial stress responseExpression of htrAFamily of serine proteasesSerine protease HtrACryo-electron tomography analysisRegulation of stress responsesLoss-of-function studiesMurine model of Lyme diseaseChemotaxis proteinsHtrA mutantVirulence determinantsDeletion mutantsFollow-up mechanistic studiesProtease HtrAPleiotropic regulatorProtease AEndogenous promoterHtrAPleiotropic rolesSerine proteasesDeletionBacterial locomotionMutantsA Helicobacter pylori flagellar motor accessory is needed to maintain the barrier function of the outer membrane during flagellar rotation
Rosinke K, Tachiyama S, Mrásek J, Liu J, Hoover T. A Helicobacter pylori flagellar motor accessory is needed to maintain the barrier function of the outer membrane during flagellar rotation. PLOS Pathogens 2025, 21: e1012860. PMID: 39792952, PMCID: PMC11756786, DOI: 10.1371/journal.ppat.1012860.Peer-Reviewed Original ResearchConceptsFlagellar rotationOuter membraneFitness defectsMutant motorsIncreased sensitivity to bacitracinWild-type growth ratesAntibiotic sensitivityPlasmid-borne copyResistant to bacitracinSensitive to bacitracinSoft agar mediumMembrane barrier functionParalyzed flagellaReduced growth rateUncharacterized lipoproteinDeletion analysisFrameshift mutationEscherichia coliAccessory proteinsAgar mediumWild typeGrowth rateMutantsH. pylori B128Flagellar motorFlbB forms a distinctive ring essential for periplasmic flagellar assembly and motility in Borrelia burgdorferi
Botting J, Rahman M, Xu H, Yue J, Guo W, Del Mundo J, Hammel M, Motaleb M, Liu J. FlbB forms a distinctive ring essential for periplasmic flagellar assembly and motility in Borrelia burgdorferi. PLOS Pathogens 2025, 21: e1012812. PMID: 39777417, PMCID: PMC11750108, DOI: 10.1371/journal.ppat.1012812.Peer-Reviewed Original ResearchConceptsComplex protein networkPeriplasmic flagellaProtein networkB. burgdorferi mutantFlagellar motorFlat-wave morphologyStator complexLyme disease spirochete Borrelia burgdorferiGroup of bacteriaCryo-electron tomographyCollar proteinFlagellar assemblyHost infectionHuman pathogensFlbBPeriplasmic ringSpirochete Borrelia burgdorferiCollar assemblyMotilityFlagellaBorrelia burgdorferiSpirochetesMolecular modelingCollarMutantsCross-modal sensory compensation increases mosquito attraction to humans
Morita T, Lyn N, von Heynitz R, Goldman O, Sorrells T, DeGennaro M, Matthews B, Houri-Zeevi L, Vosshall L. Cross-modal sensory compensation increases mosquito attraction to humans. Science Advances 2025, 11: eadn5758. PMID: 39742477, PMCID: PMC11691641, DOI: 10.1126/sciadv.adn5758.Peer-Reviewed Original Research
2024
Small molecules targeting selective PCK1 and PGC-1α lysine acetylation cause anti-diabetic action through increased lactate oxidation
Mutlu B, Sharabi K, Sohn J, Yuan B, Latorre-Muro P, Qin X, Yook J, Lin H, Yu D, Camporez J, Kajimura S, Shulman G, Hui S, Kamenecka T, Griffin P, Puigserver P. Small molecules targeting selective PCK1 and PGC-1α lysine acetylation cause anti-diabetic action through increased lactate oxidation. Cell Chemical Biology 2024, 31: 1772-1786.e5. PMID: 39341205, PMCID: PMC11500315, DOI: 10.1016/j.chembiol.2024.09.001.Peer-Reviewed Original ResearchPhosphoenolpyruvate carboxykinase 1Lysine acetylationTricarboxylic acidAnti-diabetic effectsAnaplerotic reactionsGluconeogenic reactionsLiver-specific expressionGluconeogenic metabolitesLactate oxidationSmall moleculesAnti-diabetic actionSuppressed gluconeogenesisHepatic glucose productionPGC-1aAcetylationOxaloacetateGluconeogenesisObese miceGlucose productionIncreased glucoseGlucose oxidationSubstrate oxidationOxidationGlucoseMutantsAbsence of ATG9A and synaptophysin demixing on Rab5 mutation-induced giant endosomes
Choi J, Wu Y, Park D. Absence of ATG9A and synaptophysin demixing on Rab5 mutation-induced giant endosomes. Molecular Brain 2024, 17: 63. PMID: 39223639, PMCID: PMC11367939, DOI: 10.1186/s13041-024-01132-3.Peer-Reviewed Original ResearchConceptsGiant endosomesFormation of enlarged endosomesAutophagy-related (ATG) proteinsPool of vesiclesIntegral membrane proteinsRab5 mutantsEnlarged endosomesCore autophagy-related (ATG) proteinsEndosomal localizationIntracellular sortingMembrane proteinsEndocytic originEndosomesATG9ARab5ProteinVesiclesMutantsSegregation mechanismSynapsinCo-assemblyNerve terminalsSynaptophysinFibroblastsCellsTRF2–RAP1 represses RAD51-dependent homology-directed telomere repair by promoting BLM-mediated D-loop unwinding and inhibiting BLM–DNA2-dependent 5′-end resection
Liang F, Rai R, Sodeinde T, Chang S. TRF2–RAP1 represses RAD51-dependent homology-directed telomere repair by promoting BLM-mediated D-loop unwinding and inhibiting BLM–DNA2-dependent 5′-end resection. Nucleic Acids Research 2024, 52: 9695-9709. PMID: 39082275, PMCID: PMC11381343, DOI: 10.1093/nar/gkae642.Peer-Reviewed Original ResearchHomology-directed repairTelomeric D-loopsD-loopChromosome fusionsD-loop formationSingle-stranded telomeric overhangsHomology searchTelomere clusteringTRFH domainPurified proteinBasic domainBlm mutantsProtect telomeresGenomic instabilityTelomeric overhangEnd resectionTRF2Molecular mechanismsTelomereTelomere lossMolecular pathwaysTelomere repairGenomeMutantsRap1The proteasome subunit psmb1 is essential for craniofacial cartilage maturation and morphogenesis
Miller B, Goessling W. The proteasome subunit psmb1 is essential for craniofacial cartilage maturation and morphogenesis. JCI Insight 2024, 9: e181723. PMID: 39171526, PMCID: PMC11343588, DOI: 10.1172/jci.insight.181723.Peer-Reviewed Original ResearchConceptsCraniofacial dysmorphismCraniofacial developmentMuscle differentiationProteasome mutationsTissue-autonomousMutant embryosProteasome functionConvergent extensionPalatoquadrate cartilagePSMB1Partial rescueMutantsProteasomeCraniofacial musclesMorphogenesisBirth defectsCraniofacial cartilageMuscle phenotypeDysmorphismCellular mechanismsLower jaw malformationsPhenotypeLower jawDifferentiationMuscleLipoarabinomannan mediates localized cell wall integrity during division in mycobacteria
Sparks I, Kado T, Prithviraj M, Nijjer J, Yan J, Morita Y. Lipoarabinomannan mediates localized cell wall integrity during division in mycobacteria. Nature Communications 2024, 15: 2191. PMID: 38467648, PMCID: PMC10928101, DOI: 10.1038/s41467-024-46565-5.Peer-Reviewed Original ResearchConceptsCell wall integrityWall integrityRod cell shapeCell envelope integrityHost-pathogen interactionsCell envelope componentsClinically relevant pathogensAssociated with divisionBiosynthetic mutantsEnvelope integritySubcellular locationMycobacterium smegmatisOld poleMulti-septateCell shapeMutantsBacterial modelRelevant pathogensSeptal placementPhysiological functionsMycobacterium tuberculosisEnvelope componentsMycobacteriaLipoarabinomannanDidermFluoride transport in Arabidopsis thaliana plants is impaired in Fluoride EXporter (FEX) mutants
Tausta S, Fontaine K, Hillmer A, Strobel S. Fluoride transport in Arabidopsis thaliana plants is impaired in Fluoride EXporter (FEX) mutants. Plant Molecular Biology 2024, 114: 17. PMID: 38342783, PMCID: PMC10859346, DOI: 10.1007/s11103-023-01413-w.Peer-Reviewed Original ResearchConceptsFluoride exportMutant plantsWT plantsArabidopsis thaliana plantsSingle cell eukaryotesMulticellular plantsThaliana plantsCell eukaryotesFluoride toleranceTerminal tissuesMutantsFluoride transportPlantsFloral stemsWildtypeArabidopsisEukaryotesEnvironmental toxinsStemBacteriaPositron emission tomographyPrimary locusToxinPlants distinguish different photoperiods to independently control seasonal flowering and growth
Wang Q, Liu W, Leung C, Tarté D, Gendron J. Plants distinguish different photoperiods to independently control seasonal flowering and growth. Science 2024, 383: eadg9196. PMID: 38330117, PMCID: PMC11134419, DOI: 10.1126/science.adg9196.Peer-Reviewed Original ResearchConceptsPhotoperiodic growthSeasonal floweringControl expression of genesDuration of photosynthetic activityGrowth-regulating pathwaysExpression of genesPhotosynthetic periodMeasure photoperiodPhotoperiodic floweringPhotosynthetic activityControl expressionFlowering mechanismRegulation pathwaysSeasonal growthFlowersLong daysDifferent photoperiodsPhotoperiodLight intensityPlantsGrowthMutantsGenesPathwayNoncanonical interaction with microtubules via the N-terminal nonmotor domain is critical for the functions of a bidirectional kinesin
Singh S, Siegler N, Pandey H, Yanir N, Popov M, Goldstein-Levitin A, Sadan M, Debs G, Zarivach R, Frank G, Kass I, Sindelar C, Zalk R, Gheber L. Noncanonical interaction with microtubules via the N-terminal nonmotor domain is critical for the functions of a bidirectional kinesin. Science Advances 2024, 10: eadi1367. PMID: 38324691, PMCID: PMC10849588, DOI: 10.1126/sciadv.adi1367.Peer-Reviewed Original ResearchConceptsBidirectional motilityKinesin-5Plus-end-directed motilityKinesin-5 motorsCryo-EMC-terminal tailCryo-EM mapsPlus-end-directed kinesinCryo-electron microscopyDeletion mutantsNoncanonical interactionsIntracellular functionsCin8Cryo-electronMotor domainMotilityNonmotor domainsMutantsB-tubulinSingle-moleculeCell viabilityIn vivoIn vitroIn vitro experimentsLocal defectsGPR161 structure uncovers the redundant role of sterol-regulated ciliary cAMP signaling in the Hedgehog pathway
Hoppe N, Harrison S, Hwang S, Chen Z, Karelina M, Deshpande I, Suomivuori C, Palicharla V, Berry S, Tschaikner P, Regele D, Covey D, Stefan E, Marks D, Reiter J, Dror R, Evers A, Mukhopadhyay S, Manglik A. GPR161 structure uncovers the redundant role of sterol-regulated ciliary cAMP signaling in the Hedgehog pathway. Nature Structural & Molecular Biology 2024, 31: 667-677. PMID: 38326651, PMCID: PMC11221913, DOI: 10.1038/s41594-024-01223-8.Peer-Reviewed Original ResearchConceptsCryogenic-electron microscopy structuresHedgehog pathwayTransmembrane helix 6Gpr161 functionsCiliary accumulationHeterotrimeric GsGpr161C-terminusHedgehog signalingCAMP SignalingPrimary ciliaHelix 6Signaling pathwayLoop 2Gs-mediated signalingStructural featuresExtracellular loop 2HedgehogLigand pocketGli2PathwayGs couplingSterolsMutantsSignalKey Role of Porcine Cytochrome P450 2A19 in the Bioactivation of Aflatoxin B1 in the Liver
Wang Z, Huang Q, Zhang F, Wu J, Wang L, Sun Y, Deng Y, Jiang J. Key Role of Porcine Cytochrome P450 2A19 in the Bioactivation of Aflatoxin B1 in the Liver. Journal Of Agricultural And Food Chemistry 2024, 72: 2334-2346. PMID: 38235998, DOI: 10.1021/acs.jafc.3c08663.Peer-Reviewed Original ResearchConceptsDNA damagePorcine liver microsomesStable cell linesN297A mutantProducts of AFB<sub>1</sub>Aflatoxin B<sub>1</sub>Cell deathCYP2A19DNACell linesCytochrome P450 (CYP)3ACatalytic activityProtein levelsBioactivation of aflatoxin B1Metabolic activityAdduct formationCellsMetabolic transformationAdductsLiver microsomesMutantsAFBOCYP3A46Aflatoxin B1Human-based research
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