2025
LRRK2, lysosome damage, and Parkinson's disease
Bentley-DeSousa A, Clegg D, Ferguson S. LRRK2, lysosome damage, and Parkinson's disease. Current Opinion In Cell Biology 2025, 93: 102482. PMID: 39983584, DOI: 10.1016/j.ceb.2025.102482.Peer-Reviewed Original ResearchConceptsLRRK2 kinase activityKinase activitySubstrate of LRRK2Rab familySmall GTPasesLysosomal biologyDamaged lysosomesEndolysosomal membranesRegulatory mechanismsNormal biologyPathogenesis of Parkinson's diseaseLRRK2Repair mechanismsLysosomal damageLysosomesBiologyGTPaseParkinson's diseaseRabGABARAPActivityTranslaminar synchronous neuronal activity is required for columnar synaptic strengthening in the mouse neocortex
Vargas-Ortiz J, Lin L, Martinez V, Liu R, Babij R, Duan Z, Wacks S, Sun L, Wang A, Khan S, Soto-Vargas J, De Marco García N, Che A. Translaminar synchronous neuronal activity is required for columnar synaptic strengthening in the mouse neocortex. Nature Communications 2025, 16: 1296. PMID: 39900899, PMCID: PMC11791040, DOI: 10.1038/s41467-024-55783-w.Peer-Reviewed Original ResearchThis study shows how connections across layers in the cortex synchronize early brain activity, guiding sensory development and informing strategies to address neurodevelopmental disorders.Novel Quinazoline Derivatives Inhibit Splicing of Fungal Group II Introns
Fedorova O, Luo M, Jagdmann G, Van Zandt M, Sisto L, Pyle A. Novel Quinazoline Derivatives Inhibit Splicing of Fungal Group II Introns. ACS Chemical Biology 2025, 20: 378-385. PMID: 39824511, PMCID: PMC11851433, DOI: 10.1021/acschembio.4c00631.Peer-Reviewed Original ResearchGroup II intronsRNA tertiary structureII intronsSelf-splicing group II intronsTertiary structureAntifungal activityDiscovery of small moleculesHigh-throughput screening effortsAntifungal efficacyHigh-affinity ligandsRNAIntronYeastEnhanced inhibitory activitySmall moleculesScreening methodologyCandidaInhibitory activityScreening effortsHuman healthActivityInhibitor classLigandStructureCompoundsA STING–CASM–GABARAP pathway activates LRRK2 at lysosomes
Bentley-DeSousa A, Roczniak-Ferguson A, Ferguson S. A STING–CASM–GABARAP pathway activates LRRK2 at lysosomes. Journal Of Cell Biology 2025, 224: e202310150. PMID: 39812709, PMCID: PMC11734622, DOI: 10.1083/jcb.202310150.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsApoptosis Regulatory ProteinsAutophagy-Related Protein 8 FamilyEnzyme ActivationHEK293 CellsHumansLeucine-Rich Repeat Serine-Threonine Protein Kinase-2LysosomesMembrane ProteinsMiceMicrotubule-Associated ProteinsProtein Serine-Threonine KinasesSignal TransductionConceptsLRRK2 kinase activityKinase activityStimulator of interferon genesKinase activity of LRRK2Protein family membersLysosomal recruitmentLysosomal homeostasisEndogenous cellular mechanismsAberrant activationLRRK2Interferon genesLysosomesSingle membraneLysosomal damageMultiple chemical stimuliKinaseCellular mechanismsPathwayFamily membersChemical stimuliGABARAPMultiple stimuliGenesMutationsActivity
2024
Energy partitioning in the cell cortex
Chen S, Seara D, Michaud A, Kim S, Bement W, Murrell M. Energy partitioning in the cell cortex. Nature Physics 2024, 20: 1824-1832. DOI: 10.1038/s41567-024-02626-6.Peer-Reviewed Original ResearchCell cortexEntropy production rateGAP expressionCortical actin filamentsRho GTPase pathwayGTPase pathwayMyosin IIActin filamentsDiversity patternsEnergy partitioningRhoOnsager reciprocityCell phenotypeProtein expressionThermodynamic equilibriumCellsSpiral travelling waveProduction rateTemporal dynamicsLiving systemsActinEnergyWavePhenotypeActivityApplications of cell free protein synthesis in protein design
Thornton E, Paterson S, Stam M, Wood C, Laohakunakorn N, Regan L. Applications of cell free protein synthesis in protein design. Protein Science 2024, 33: e5148. PMID: 39180484, PMCID: PMC11344276, DOI: 10.1002/pro.5148.Peer-Reviewed Original Research
2023
Metformin inhibits digestive proteases and impairs protein digestion in mice
Kelly C, Verdegaal A, Anderson B, Shaw W, Bencivenga-Barry N, Folta-Stogniew E, Goodman A. Metformin inhibits digestive proteases and impairs protein digestion in mice. Journal Of Biological Chemistry 2023, 299: 105363. PMID: 37863262, PMCID: PMC10663847, DOI: 10.1016/j.jbc.2023.105363.Peer-Reviewed Original ResearchConceptsGastrointestinal side effectsSide effectsDrug concentrationsDaily metformin doseFirst-line therapyType 2 diabetesEnteropeptidase activityPrescribed medicationsMetformin doseIntestinal lumenGastrointestinal tissuesMice exhibitMetforminProtein maldigestionHuman duodenumProtein digestionTrypsin activityDigestive enzymesMedicationsDiabetesMaldigestionDuodenumTherapyActivityMiceStructure-function analysis of ceTIR-1/hSARM1 explains the lack of Wallerian axonal degeneration in C. elegans
Khazma T, Grossman A, Guez-Haddad J, Feng C, Dabas H, Sain R, Weitman M, Zalk R, Isupov M, Hammarlund M, Hons M, Opatowsky Y. Structure-function analysis of ceTIR-1/hSARM1 explains the lack of Wallerian axonal degeneration in C. elegans. Cell Reports 2023, 42: 113026. PMID: 37635352, PMCID: PMC10675840, DOI: 10.1016/j.celrep.2023.113026.Peer-Reviewed Original ResearchConceptsC. elegansCryoelectron microscopy structureNematode C. elegansC. elegans neuronsStructure-function analysisMicroscopy structureNADase activityMolecular mechanismsElegansCellular NADModel animalsSpeciesAxon degenerationWallerian axonal degenerationOrthologsOctamerProteinSARM1DivergenceNADSARMExpressionActivityAxonal degenerationEffects of CYP3A4 and CYP2C9 genotype on systemic anastrozole and fulvestrant concentrations in SWOG S0226
Rutherford D, Medley S, Henderson N, Gersch C, Vandenberg T, Albain K, Dakhil S, Tirumali N, Gralow J, Hortobagyi G, Pusztai L, Mehta R, Hayes D, Kidwell K, Henry N, Barlow W, Rae J, Hertz D. Effects of CYP3A4 and CYP2C9 genotype on systemic anastrozole and fulvestrant concentrations in SWOG S0226. Pharmacogenomics 2023, 24: 665-673. PMID: 37615099, PMCID: PMC10565537, DOI: 10.2217/pgs-2023-0097.Peer-Reviewed Original ResearchNeighborhood Social Environment and Body Mass Index: The Mediating Role of Mental Wellbeing
Cunningham S, Mandelbaum J, Shebl F, Abraham M, Duffany K. Neighborhood Social Environment and Body Mass Index: The Mediating Role of Mental Wellbeing. International Journal Of Environmental Research And Public Health 2023, 20: 6602. PMID: 37623185, PMCID: PMC10454589, DOI: 10.3390/ijerph20166602.Peer-Reviewed Original ResearchConceptsBody mass indexNeighborhood social environmentPhysical activityMass indexMental wellbeingLow physical activityNeighborhood-built environmentObesity riskObesogenic behaviorsBehavioral interventionsPrevention effortsPopulation healthSignificant totalZip codesRandom sampleAssociationStudy findingsFurther supportWellbeingSocial environmentActivityThe EN-TEx resource of multi-tissue personal epigenomes & variant-impact models
Rozowsky J, Gao J, Borsari B, Yang Y, Galeev T, Gürsoy G, Epstein C, Xiong K, Xu J, Li T, Liu J, Yu K, Berthel A, Chen Z, Navarro F, Sun M, Wright J, Chang J, Cameron C, Shoresh N, Gaskell E, Drenkow J, Adrian J, Aganezov S, Aguet F, Balderrama-Gutierrez G, Banskota S, Corona G, Chee S, Chhetri S, Cortez Martins G, Danyko C, Davis C, Farid D, Farrell N, Gabdank I, Gofin Y, Gorkin D, Gu M, Hecht V, Hitz B, Issner R, Jiang Y, Kirsche M, Kong X, Lam B, Li S, Li B, Li X, Lin K, Luo R, Mackiewicz M, Meng R, Moore J, Mudge J, Nelson N, Nusbaum C, Popov I, Pratt H, Qiu Y, Ramakrishnan S, Raymond J, Salichos L, Scavelli A, Schreiber J, Sedlazeck F, See L, Sherman R, Shi X, Shi M, Sloan C, Strattan J, Tan Z, Tanaka F, Vlasova A, Wang J, Werner J, Williams B, Xu M, Yan C, Yu L, Zaleski C, Zhang J, Ardlie K, Cherry J, Mendenhall E, Noble W, Weng Z, Levine M, Dobin A, Wold B, Mortazavi A, Ren B, Gillis J, Myers R, Snyder M, Choudhary J, Milosavljevic A, Schatz M, Bernstein B, Guigó R, Gingeras T, Gerstein M. The EN-TEx resource of multi-tissue personal epigenomes & variant-impact models. Cell 2023, 186: 1493-1511.e40. PMID: 37001506, PMCID: PMC10074325, DOI: 10.1016/j.cell.2023.02.018.Peer-Reviewed Original Research
2022
Serological fingerprints link antiviral activity of therapeutic antibodies to affinity and concentration
Fiedler S, Devenish S, Morgunov A, Ilsley A, Ricci F, Emmenegger M, Kosmoliaptsis V, Theel E, Mills J, Sholukh A, Aguzzi A, Iwasaki A, Lynn A, Knowles T. Serological fingerprints link antiviral activity of therapeutic antibodies to affinity and concentration. Scientific Reports 2022, 12: 19791. PMID: 36396691, PMCID: PMC9672333, DOI: 10.1038/s41598-022-22214-z.Peer-Reviewed Original ResearchConceptsSerum antibody responseAntiviral activityHigh antiviral activityAntibody responseTherapeutic mAbsReduced antiviral activityVirus neutralization assaysSARS-CoV-2 virusSARS-CoV-2Convalescent individualsNeutralization assaysTherapeutic monoclonal antibodiesSame mAbMonoclonal antibodiesNew therapeuticsTherapeutic antibodiesMAbsAntibodiesRBDSotrovimabWild typeActivitySerumProtocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling
Hsu JC, Pawlak JB, Laurent-Rolle M, Cresswell P. Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling. STAR Protocols 2022, 3: 101654. PMID: 36072758, PMCID: PMC9442383, DOI: 10.1016/j.xpro.2022.101654.Peer-Reviewed Original ResearchConceptsTranslational regulationMammalian cell linesDifferent cell typesDifferent growth conditionsMammalian cellsTranslation activityGene expressionBiological processesCell typesComplete detailsConfocal microscopyCell linesGrowth conditionsPuromycin analoguesCritical roleRegulationCellsProteinLabelingFundamental stepExpressionClick chemistry reactionActivityCytometryEpileptiform activity predicts epileptogenesis in cerebral hemorrhage
Kong THJ, Azeem M, Naeem A, Allen S, Kim JA, Struck AF. Epileptiform activity predicts epileptogenesis in cerebral hemorrhage. Annals Of Clinical And Translational Neurology 2022, 9: 1475-1480. PMID: 36030385, PMCID: PMC9463945, DOI: 10.1002/acn3.51637.Peer-Reviewed Original ResearchConceptsEpileptiform activitySubarachnoid hemorrhageRisk factorsRetrospective case-control studyNontraumatic intraparenchymal hemorrhagesDevelopment of epilepsyCase-control studyS scoresClinical seizuresAcute phaseCerebral hemorrhageIntraparenchymal hemorrhageHemorrhageSeizuresEpilepsyScoresEpileptogenesisPatientsActivityIntraparenchymalImproving Breast Cancer Family Caregivers’ Palliative Care Literacy: A Pilot Randomized Trial
Schulman-Green D, Linsky S, Blatt L, Jeuland J, Kapo J, Jeon S. Improving Breast Cancer Family Caregivers’ Palliative Care Literacy: A Pilot Randomized Trial. Journal Of Family Nursing 2022, 29: 99-114. PMID: 35670155, DOI: 10.1177/10748407221099541.Peer-Reviewed Original ResearchConceptsCancer family caregiversCaregiver burdenFamily caregiversPilot Randomized TrialRandomized trialsMean agePreliminary efficacyCancer carePatient goalsIntervention participantsCare conversationsCare communicationCaregiver activitiesFurther studiesTrialsCaregiversBurdenTraining programParticipantsPatientsEthnic minoritiesIllnessFamily managementActivityLack of goals
2021
Frequency modulation of entorhinal cortex neuronal activity drives distinct frequency-dependent states of brain-wide dynamics
Salvan P, Lazari A, Vidaurre D, Mandino F, Johansen-Berg H, Grandjean J. Frequency modulation of entorhinal cortex neuronal activity drives distinct frequency-dependent states of brain-wide dynamics. Cell Reports 2021, 37: 109954. PMID: 34731612, PMCID: PMC8609366, DOI: 10.1016/j.celrep.2021.109954.Peer-Reviewed Original ResearchConceptsBrain-wide dynamicsFrequency-dependent fashionEntorhinal cortex dysfunctionHuman neuroimaging studiesBasic neuronal mechanismsEntorhinal cortex activityExcitatory neuronsTransgenic modelStimulation frequencyCortex activityFMRI fluctuationsNeuronal mechanismsAnatomical circuitsNeuroimaging studiesNeural activityNeuronal spikingCognitive diseasesFMRI analysisDysfunctionModulationDiseaseMiceNeuronsActivityBrainFrom implementation to sustainment: A large-scale adverse event disclosure support program generated through embedded research in the Veterans Health Administration
Elwy AR, Maguire EM, McCullough M, George J, Bokhour BG, Durfee JM, Martinello RA, Wagner TH, Asch SM, Gifford AL, Gallagher TH, Walker Y, Sharpe VA, Geppert C, Holodniy M, West G. From implementation to sustainment: A large-scale adverse event disclosure support program generated through embedded research in the Veterans Health Administration. Healthcare 2021, 8: 100496. PMID: 34175102, PMCID: PMC11365187, DOI: 10.1016/j.hjdsi.2020.100496.Peer-Reviewed Original ResearchConceptsVeterans Health AdministrationHealth AdministrationVA healthcare systemResearch-operations partnershipsLarge-scale adverse eventsAdverse eventsVeteran patientsVA healthcareConsolidated FrameworkSupport programsImplementation researchHealthcare systemToolkit usePatientsAdministrationOperational leadersVA operationsClinical operationsToolkit implementationMore harmHarmActivityMeasurement of Motivation States for Physical Activity and Sedentary Behavior: Development and Validation of the CRAVE Scale
Stults-Kolehmainen MA, Blacutt M, Fogelman N, Gilson TA, Stanforth PR, Divin AL, Bartholomew JB, Filgueiras A, McKee PC, Ash GI, Ciccolo JT, Decker L, Williamson SL, Sinha R. Measurement of Motivation States for Physical Activity and Sedentary Behavior: Development and Validation of the CRAVE Scale. Frontiers In Psychology 2021, 12: 568286. PMID: 33841225, PMCID: PMC8027339, DOI: 10.3389/fpsyg.2021.568286.Peer-Reviewed Original ResearchPhysical activitySedentary behaviorMaximal treadmill testInterclass correlationCommunity-residing participantsTreadmill testRest relateGood psychometric propertiesPhysical fatigueExploratory structural equation modelTime pointsState-like qualitiesYear (based on the entire 80 yrs is a) two timesExercise behaviorShort AssessmentConstruct validityPsychometric propertiesFuture studiesParticipantsRestIndependent samplesActivityTirednessTwo-Photon Fluorescent Probes for Detecting Enzyme Activities in Live Tissues
Juvekar V, Lee H, Kim H. Two-Photon Fluorescent Probes for Detecting Enzyme Activities in Live Tissues. ACS Applied Bio Materials 2021, 4: 2957-2973. PMID: 35014386, DOI: 10.1021/acsabm.1c00063.Peer-Reviewed Original ResearchConceptsEnzyme activityDetectable enzyme activityEnzymatic probesEnzyme regulationBiological significanceEfficient detection toolAbnormal enzyme activityEnzymeAlzheimer's diseasePhysiological functionsLiving organismsTwo-photon microscopyProbe developmentBiological systemsEnzyme sensorEnzyme investigationsMinimal perturbationFluorescent probeLiving systemsReal-time bioimagingBiosynthesesTwo-photon fluorescent probeActivityDetection toolsLiving tissues
2020
Assessing community health research capacity across stakeholders: adapting a tool
Humphries D, Gupta R, Dukpa T, Wangmo D. Assessing community health research capacity across stakeholders: adapting a tool. Health Promotion International 2020, 36: 1198-1208. PMID: 33270872, DOI: 10.1093/heapro/daaa105.Peer-Reviewed Original ResearchConceptsHealth research capacityResearch capacity building activitiesCommunity health research projectsCommunity health researchStrong community engagementHealth research projectsResearch capacityHealth disparitiesResearch assessment toolHealth research stakeholdersPerson interviewsHealth researchValid toolAssessment toolResearch NetworkStrong internal reliabilityInternal reliabilityParticipantsCollaborative approachSkilled staffCliniciansStaffStakeholder organizationsActivity
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