2009
Melanin-concentrating hormone directly inhibits GnRH neurons and blocks kisspeptin activation, linking energy balance to reproduction
Wu M, Dumalska I, Morozova E, van den Pol A, Alreja M. Melanin-concentrating hormone directly inhibits GnRH neurons and blocks kisspeptin activation, linking energy balance to reproduction. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 17217-17222. PMID: 19805188, PMCID: PMC2761345, DOI: 10.1073/pnas.0908200106.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainEnergy MetabolismFemaleGonadotropin-Releasing HormoneGreen Fluorescent ProteinsHypothalamic HormonesImmunohistochemistryKisspeptinsMaleMelaninsMembrane PotentialsMiceMice, TransgenicMicroscopy, FluorescenceNeuronsPatch-Clamp TechniquesPeptide FragmentsPituitary HormonesReceptors, SomatostatinReproductionTime FactorsTumor Suppressor ProteinsVesicular Glutamate Transport Protein 2ConceptsInhibitory effectDirect postsynaptic effectGnRH-GFP neuronsMCH knockout miceMCH-immunoreactive fibersRole of MCHMelanin-concentrating hormone (MCH) systemMCH receptor antagonistsPatch-clamp recordingsMelanin-concentrating hormoneNegative energy balanceKisspeptin activationDrug abuse behaviorGnRH neuronsPostsynaptic effectsExcitatory effectsGABAergic neuronsTransgenic GFP miceImmunoreactive fibersReceptor antagonistCNS neuronsAntidepressant drugsBrain slicesFood restrictionKnockout mice
2008
Excitatory Effects of the Puberty-Initiating Peptide Kisspeptin and Group I Metabotropic Glutamate Receptor Agonists Differentiate Two Distinct Subpopulations of Gonadotropin-Releasing Hormone Neurons
Dumalska I, Wu M, Morozova E, Liu R, van den Pol A, Alreja M. Excitatory Effects of the Puberty-Initiating Peptide Kisspeptin and Group I Metabotropic Glutamate Receptor Agonists Differentiate Two Distinct Subpopulations of Gonadotropin-Releasing Hormone Neurons. Journal Of Neuroscience 2008, 28: 8003-8013. PMID: 18685025, PMCID: PMC2597556, DOI: 10.1523/jneurosci.1225-08.2008.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBarium CompoundsCapillariesChloridesDiagonal Band of BrocaDrug ResistanceElectrophysiologyFemaleGonadotropin-Releasing HormoneGreen Fluorescent ProteinsIn Vitro TechniquesMaleMedian EminenceMethoxyhydroxyphenylglycolMiceMice, TransgenicNeuronsPotassium ChannelsRatsRats, Sprague-DawleyReceptors, Metabotropic GlutamateRNA, MessengerSynapsesTumor Suppressor ProteinsVesicular Glutamate Transport Protein 2ConceptsMetabotropic glutamate receptor agonistGlutamate receptor agonistsGroup I metabotropic glutamate receptor agonistReceptor agonistGnRH neuronsGonadotropin-Releasing Hormone NeuronsG protein-coupled receptor GPR54Vesicular glutamate transporter 2GnRH-GFP miceRetrograde labeling techniqueGlutamate transporter 2Gonadotropin-releasing hormoneDistinct subpopulationsOnset of pubertyPostpubertal miceHormone neuronsGnRH cellsExcitatory effectsCentral releaseHypogonadotropic hypogonadismPeptide kisspeptinReceptor GPR54Brain slicesNormal pubertyKisspeptin