2007
A Calcium- and Calmodulin-Dependent Kinase Iα/Microtubule Affinity Regulating Kinase 2 Signaling Cascade Mediates Calcium-Dependent Neurite Outgrowth
Uboha NV, Flajolet M, Nairn AC, Picciotto MR. A Calcium- and Calmodulin-Dependent Kinase Iα/Microtubule Affinity Regulating Kinase 2 Signaling Cascade Mediates Calcium-Dependent Neurite Outgrowth. Journal Of Neuroscience 2007, 27: 4413-4423. PMID: 17442826, PMCID: PMC6672303, DOI: 10.1523/jneurosci.0725-07.2007.Peer-Reviewed Original ResearchConceptsKinase domainNeurite outgrowthNeuronal differentiationCalmodulin-dependent kinase ITwo-hybridNeuro-2a cellsPhosphorylation sitesSignal transductionKinase ICaMKIKinase activityMicrotubule affinityKinase 2Calcium-dependent pathwayCritical regulatorIndependent screensPrimary hippocampal neuronsNovel siteCalcium ionophore ionomycinOutgrowthIonophore ionomycinSynaptic plasticityDifferentiationHippocampal neuronsPhysiological effects
2004
Cytoplasmic localization of calcium/calmodulin‐dependent protein kinase I‐α depends on a nuclear export signal in its regulatory domain
Stedman DR, Uboha NV, Stedman TT, Nairn AC, Picciotto MR. Cytoplasmic localization of calcium/calmodulin‐dependent protein kinase I‐α depends on a nuclear export signal in its regulatory domain. FEBS Letters 2004, 566: 275-280. PMID: 15147908, DOI: 10.1016/j.febslet.2004.04.042.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsCalcium-Calmodulin-Dependent Protein Kinase Type 1Calcium-Calmodulin-Dependent Protein KinasesConsensus SequenceCytoplasmEnzyme ActivationKaryopherinsMicroscopy, FluorescenceNuclear Localization SignalsPC12 CellsProtein Structure, TertiaryRatsReceptors, Cytoplasmic and NuclearRecombinant Fusion ProteinsSubcellular FractionsTransfectionConceptsNuclear export signalExport signalRegulatory domainCytoplasmic localizationCalmodulin-dependent protein kinase ICalcium/calmodulin-dependent protein kinase IRev-like nuclear export signalProtein kinase IUbiquitous cytosolic enzymeCalcium/calmodulinTranscriptional regulationNuclear exportKinase INuclear proteinsNuclear entryCatalytic coreCytosolic enzymeI alphaSequenceCRM1VitroMutantsLocalizationCalmodulinDomain
1996
Structure, Regulation, and Function of Calcium/Calmodulin-Dependent Protein Kinase I
Picciotto M, Nastiuk K, Nairn A. Structure, Regulation, and Function of Calcium/Calmodulin-Dependent Protein Kinase I. Advances In Pharmacology 1996, 36: 251-275. PMID: 8783563, DOI: 10.1016/s1054-3589(08)60585-2.Peer-Reviewed Original ResearchConceptsProtein kinaseProtein kinase CMyosin light chain kinaseKinase ICaM kinaseSecond messenger-regulated protein kinasesCalmodulin-dependent protein kinase ICalcium/calmodulin-dependent protein kinase ICAMP-dependent protein kinaseSpecific subcellular locationsMultifunctional protein kinaseTerminal regulatory domainDependent protein kinaseCaM kinase familyClass of enzymesProtein kinase ICaM kinase IAmino acid residuesMyosin P-light chainDomain bindsAutoinhibitory mechanismRegulatory domainKinase familyProtein phosphorylationLight chain kinase
1995
The Regulatory Region of Calcium/Calmodulin-dependent Protein Kinase I Contains Closely Associated Autoinhibitory and Calmodulin-binding Domains (∗)
Yokokura H, Picciotto M, Nairn A, Hidaka H. The Regulatory Region of Calcium/Calmodulin-dependent Protein Kinase I Contains Closely Associated Autoinhibitory and Calmodulin-binding Domains (∗). Journal Of Biological Chemistry 1995, 270: 23851-23859. PMID: 7559563, DOI: 10.1074/jbc.270.40.23851.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCalcium-Calmodulin-Dependent Protein Kinase Type 1Calcium-Calmodulin-Dependent Protein KinasesCalmodulinDNA, ComplementaryEnzyme InhibitorsIn Vitro TechniquesMolecular Sequence DataMutagenesisMyosin-Light-Chain KinaseRatsRecombinant Fusion ProteinsSequence DeletionSequence Homology, Amino AcidStructure-Activity RelationshipConceptsCaM kinase IKinase IProtein kinase ITruncation mutantsCalmodulin-dependent protein kinase ICalcium/calmodulin-dependent protein kinase IDependent protein kinase IDependent protein kinaseSyntide-2Active kinaseAutoinhibitory domainDependent activityGlutathione S-transferaseProtein kinaseRegulatory regionsActive mutantMutantsFusion proteinPeptide substratesIntrasteric mechanismGlutathione-Sepharose 4B.COOH-terminalS-transferaseImmunochemical localization of calcium/calmodulin‐dependent protein kinase I
Picciotto M, Zoli M, Bertuzzi G, Nairn A. Immunochemical localization of calcium/calmodulin‐dependent protein kinase I. Synapse 1995, 20: 75-84. PMID: 7624832, DOI: 10.1002/syn.890200111.Peer-Reviewed Original ResearchConceptsKinase IProtein kinase ICaM kinase INon-neuronal tissuesImmunoreactive speciesCalmodulin-dependent protein kinase IGlutathione S-transferase fusion proteinCalcium/calmodulin-dependent protein kinase IRat brainDependent protein kinase ISubcellular fractionation studiesRecombinant kinasesRat brain enzymeNeuronal cell bodiesCytosolic localizationProtein kinaseMultiple immunoreactive speciesMajor immunoreactive speciesFusion proteinMultiple neuronal processesWidespread cellMajor immunoreactive bandRat cDNAPrimary structureSynapsin I.P3-52 IDENTIFICATION OF AUTOINHIBITORY AND CALMODULIN-RINDING DOMAINS ON CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE I
Yokokura H, Picciotto M, Nairn A, Hidaka H. P3-52 IDENTIFICATION OF AUTOINHIBITORY AND CALMODULIN-RINDING DOMAINS ON CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE I. Journal Of Pharmacological Sciences 1995, 67: 259. DOI: 10.1016/s0021-5198(19)47002-9.Peer-Reviewed Original Research
1994
Calcium/calmodulin-dependent protein kinases.
Nairn AC, Picciotto MR. Calcium/calmodulin-dependent protein kinases. Seminars In Cancer Biology 1994, 5: 295-303. PMID: 7803766.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCalcium-Calmodulin-Dependent Protein Kinase Type 1Calcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein Kinase Type 4Calcium-Calmodulin-Dependent Protein KinasesElongation Factor 2 KinaseHumansMolecular Sequence DataMyosin-Light-Chain KinasePhosphorylationConceptsProtein kinaseSecond messenger-regulated protein kinasesCaM-dependent protein kinaseEssential intracellular second messengerEF-2 kinaseCaM kinase ICaM kinase IVCaM kinase IIIntracellular second messengerMyosin light chain kinaseEukaryotic systemsProtein phosphorylationKinase ILight chain kinaseKinase IIPhosphorylase kinaseGene expressionKinase IVSecond messengerKinaseChain kinaseImportant familyCell proliferationNeurotransmitter releaseRegulation
1993
Calcium/calmodulin-dependent protein kinase I. cDNA cloning and identification of autophosphorylation site.
Picciotto MR, Czernik AJ, Nairn AC. Calcium/calmodulin-dependent protein kinase I. cDNA cloning and identification of autophosphorylation site. Journal Of Biological Chemistry 1993, 268: 26512-26521. PMID: 8253780, DOI: 10.1016/s0021-9258(19)74343-9.Peer-Reviewed Original ResearchMeSH KeywordsAdrenal GlandsAmino Acid SequenceAnimalsBase SequenceBinding SitesBrainCalcium-Calmodulin-Dependent Protein Kinase Type 1Calcium-Calmodulin-Dependent Protein KinasesCattleCloning, MolecularDNA, ComplementaryEscherichia coliLiverLungMolecular Sequence DataPhosphorylationRatsRNA, MessengerSequence Homology, Amino AcidConceptsCaM kinase IKinase IProtein kinaseCatalytic domainThreonyl residuesFusion proteinGlutathione S-transferase fusion proteinS-transferase fusion proteinCAMP-dependent protein kinaseDependent protein kinase IComplete amino acid sequenceBovine brain cDNA libraryInvariant amino acidsAmino acidsSynapsin IAmino acid sequenceBrain cDNA libraryClass of enzymesSynaptic vesicle proteinsProtein kinase ICaM kinase IIAutophosphorylation sitesRNase protection assaysSingle geneCDNA library
1992
Phosphorylation of the cystic fibrosis transmembrane conductance regulator.
Picciotto MR, Cohn JA, Bertuzzi G, Greengard P, Nairn AC. Phosphorylation of the cystic fibrosis transmembrane conductance regulator. Journal Of Biological Chemistry 1992, 267: 12742-12752. PMID: 1377674, DOI: 10.1016/s0021-9258(18)42339-3.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorProtein kinase CFibrosis transmembrane conductance regulatorProtein kinaseTransmembrane conductance regulatorR domainRegulation of CFTRCalmodulin-dependent protein kinase IConductance regulatorCalcium/calmodulin-dependent protein kinase IDirect amino acid sequencingCyclic AMP-dependent protein kinaseCyclic GMP-dependent protein kinaseAMP-dependent protein kinaseGMP-dependent protein kinaseCF 2Peptide mappingProtein kinase IAmino acid sequencingDifferent second messenger pathwaysSecond messenger pathwaysDirect phosphorylationKinase ISerine 660Seryl residues