Processing of proTRH to its intermediate products occurs before the packing into secretory granules of transfected AtT20 cells
Nillni E, Sevarino K, Jackson I. Processing of proTRH to its intermediate products occurs before the packing into secretory granules of transfected AtT20 cells. Endocrinology 1993, 132: 1271-1277. DOI: 10.1210/en.132.3.1271.Peer-Reviewed Original ResearchPulse-chase studiesProcessing of proTRHSecretory granulesAtT20 cellsAssay of marker enzymesPolyacrylamide gel electrophoresisPulse-chase experimentsCryptic peptidesC-terminal processing productsNitrogen cavitationSubcellular compartmentsSG fractionGel electrophoresisRER fractionPosttranslational processingSubcellular fractionsIntracellular compartmentsPurified antibodiesProTRH processingIntermediate processing productsGolgiIntact precursorMarker enzymesPeptideProcessing products