BD FACSAria 300 GS-345
Location: Room 345 at 300 George Street
Contact: (203)737-7451, (203)785-2031 lesley.devine@yale.edu
(203)785-2031 Chao.Wang@yale.edu
This FACS Aria is available for all sample types but is particularly well positioned to handle clinical human samples on same day notice if the samples are fully prepared by the investigator. Clinical samples requiring cell sorting that arise unexpectedly can be accommodated during regular working hours if the cell number is small, or after hours if the samples are larger. For this reason, all investigators should be aware that the start time of their scheduled sort on this instrument might be delayed as much as an hour. Cell sorting on this Aria should be avoided if your project requires a rigid schedule.
Violet Laser - 405nm
PMT | Band Pass | Long Pass | Fluorochrome |
A | 530/30 | 505LP | AmCyan, V500, BV510 |
B | 450/50 |
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Pacific Blue, DAPI, BV421, V450 |
Red Laser - 633nm
PMT | Bandpass | Long Pass | Fluorochrome |
A | 780/60 | 735 LP | APC-Cy7, APC-H7, Alexa Fluor 750 |
B | 660/20 |
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APC, Alexa-647 |
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Blue Laser - 488nm
PMT | Bandpass | Long Pass | Fluorochrome |
A | 780/60 | 735 LP | PE-Cy7 |
B | 695/40 | 655 LP | PE-Cy5.5, PerCP, PerCP-Cy5.5 |
C | 610/20 | 595 LP | PE-Texas Red |
D | 575/26 | 556 LP | PE |
E | 530/30 | 502 LP | FITC |