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Instrumentation

For analysis

There are 6 user-operated analyzers in the TAC Building (LSRII TAC5-UV, LSRII Green, LSRII TAC6-GS, two CytoFlex LX cytometers and BD Symphony). In Amistad there is a LSRII and FACSCalibur. The Caliburs can analyze up to 4 colors with 488 nm and 633 nm excitation lasers. 300 George Street location is equipped with Symphony B and two BD LSR Fortessa's. LSRII TAC5-UV and LSRII Green have a capability of 14 and 15 colors. LSRII Green, CytoFlex LX A and B and Symphony A are located in room TAC S613. LSRII TAC6-GS is in room TAC S617. LSRII TAC5 UV is in room TAC S533. Machines are checked daily for optimal performance. Internet sign up for analysis time is available for users after completing the training.

Amnis Imagestream-X imaging flow cytometer (room S613) provides users with the ability to gain detailed images of a large number of cells in a relatively short period of time and with the opportunity to perform a range of novel applications including co-localization, internalization, stem cell differentiation, and cell-cell interactions. For training please contact Diane.Trotta@yale.edu.

Luminex 200 analyzer is located at 300 George St., Rm 345

For cell sorting

FACSArias in TAC S633, Amistad and George St., and SONY SH800 are operated by staff or can be user-operated after completing the training.

Cells can be sorted into 5 or 15 ml tubes or into various plates as single or multiple cells per well or onto microscopic slides for analysis. In addition, all sorters (except SONY SH800)are capable of simultaneous 4–way sorting. All sorters except MoFlo are also equipped for sorting live human, primate or other potentially biohazardous cells.