BD LSR Fortessa
Location: 300 George Street, rooms 345 and 345A
Contact: Lesley.Devine@yale.edu
The Fortessas are within rooms that are designated BSL2 space to permit the sorting and analysis of unfixed human samples from healthy individuals. Although gloves are sufficient PPE when running non-human samples, please note that analysis of unfixed human samples requires additional PPE (face shield, user provided) and equipment disinfection steps then is needed at other cytometers. Personal protection should be taken seriously in these rooms. Human samples from individuals known to be infected with HIV or HepB/C must be fixed prior to analysis at any cytometer including these Fortessas.
Violet Laser - 405nm 100 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 640/40 | 635LP | Qdot 655, BV650 |
| B | 525/50 | 505LP | AmCyan, V500, BV510 |
| C | 450/50 |
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Pacific Blue, DAPI, BV421, V450 |
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Blue Laser - 488nm 50 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 685/35 | 670LP | PerCP, PerCP-Cy5.5 |
| B | 515/20 | 505LP | FITC, GFP, CFSE |
| C | 488/10 | SSC |
Green Laser - 532nm 150 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/40 | 735LP | PE-Cy7 |
| B | 710/50 | 685LP | PE-Cy5.5 |
| C | 660/20 | 635LP | PE-Cy5 |
| D | 610/20 | 600LP | PE-Texas Red, PI
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| E | 582/15 | PE |
Red Laser - 640 nm 100 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/60 | 750LP | APC-Cy7, APC-H7, APC-Alexa Fluor 750 |
| B | 730/45 | 685LP | Alexa Fluor 680, Alexa Fluor 700 |
| C | 670/30 | APC, Alexa Fluor 647 |