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INFORMATION FOR

BD LSR Fortessa

Location: 300 George Street, rooms 345 and 345A

Contact: Lesley.Devine@yale.edu

The Fortessas are within rooms that are designated BSL2 space to permit the sorting and analysis of unfixed human samples from healthy individuals. Although gloves are sufficient PPE when running non-human samples, please note that analysis of unfixed human samples requires additional PPE (face shield, user provided) and equipment disinfection steps then is needed at other cytometers. Personal protection should be taken seriously in these rooms. Human samples from individuals known to be infected with HIV or HepB/C must be fixed prior to analysis at any cytometer including these Fortessas.


Violet Laser - 405nm 100 mW

PMT Bandpass Long Pass Fluorochrome
A 640/40 635LP Qdot 655, BV650
B 525/50 505LP AmCyan, V500, BV510
C 450/50
Pacific Blue, DAPI, BV421, V450



Blue Laser - 488nm 50 mW

PMT Bandpass Long Pass Fluorochrome
A 685/35 670LP PerCP, PerCP-Cy5.5
B 515/20 505LP FITC, GFP, CFSE
C 488/10 SSC

Green Laser - 532nm 150 mW

PMT Bandpass Long Pass Fluorochrome
A 780/40 735LP PE-Cy7
B 710/50 685LP PE-Cy5.5
C 660/20 635LP PE-Cy5
D 610/20 600LP PE-Texas Red, PI
E 582/15 PE

Red Laser - 640 nm 100 mW

PMT Bandpass Long Pass Fluorochrome
A 780/60 750LP APC-Cy7, APC-H7, APC-Alexa Fluor 750
B 730/45 685LP Alexa Fluor 680, Alexa Fluor 700
C 670/30 APC, Alexa Fluor 647