Contact: Lesley.Devine@yale.edu (203) 785-2031
The Luminex is capable of measuring up to 100 analytes. Commercially available kits can be used for the detection of multiple cytokines in serum, plasma or tissue culture supernatants. Kits consist of beads dyed with differing concentrations of two fluorophores to generate distinct bead sets. Each bead set is coated with a capture antibody specific for a specific cytokine. A second biotinylated antibody and streptavidin-phycoerythrin (S-PE) is used to detect the captured cytokine. The Luminex 200 analyzer is a dual laser, flow-based, sorting and detection platform. One laser detects the beads and determines which cytokine is being detected. The second laser determines the magnitude of PE-derived signal, which is proportional to the amount of cytokine bound.