CytoFlex LX A and B
Both CytoFLEX analyzers are a five laser system capable of detecting in 19 different channels (please see the configuration below for details), with a slight difference: CytoFLEX A has a VSSC detector off the violet laser to allow for small particle detection, while CytoFLEX B has a BV786 detector. Otherwise both instruments have the same configuration.
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The CytoFLEXes are equipped with a 561 green laser, this is different from the Stratedigms, which they have replaced and our LSRIIs with 532 green lasers.
- You should expect your PE and PE-tandem dyes to appear brighter than they did before. Less antibody might be required to avoid extreme adjustments on the sensitive detectors of this model.
- Some fluorescent proteins that were barely detectable with a 532 nm laser will appear significantly brighter with 561 nm excitation. mCherry, mKate, mKatushka, and mRaspberry will perform much better with this laser line.
CytoflexLX A and B have almost the same configurations. Only one detector difference off violet laser. VSSC vs. BV786
|Laser (NM)||Bandpass||Channel Name||Additional Optimal Colors|
|450/45||DAPI||ZombieUV, L/DFix Blue, DyLight 350|
|405||405/10||VSSC (CytoA only)||Violet Side Scatter - used for small particle detection|
|450/45||PacificBlue||BV421, V450, Alexa405, CF405M, DyLight405, eFluor450, SuperBright436, VioBlue, Zombie Violet, L/DFixViolet|
|525/40||BV510||L/DFix Aqua, V500, AF430, DyLight405LS, VioGreen, Zombie Aqua|
|610/20||BV605||Qdot605, DyLight405LS, eFluor605NC, eVolve605, SuperBright600|
|763/43||BV786 (CytoB only)||Qdot800|
|488||525/40||FITC||GFP, Alexa488, CFSE, BB515, DyLight488, Zombie Green, L/Dfix Green|
|610/20||B610-ECD||all current available fluors for this channel are best excited by the 561 laser|
|690/50||PerCP||PerCP-Cy5.5, BB700, PerCP-eFluor710, PerCP-Vio700|
|610/20||PE-TexasRed||mCherry, PE-Dazzle, PE-CF594, PE-Vio615, PE-eFluor610, DyLight594, Zombie Red, L/Dfix Red|
|638||660/10||APC||AF647, Dylight633, L/Dfix Far Red|
|763/43||APC-Cy7||APC-Fire750, APC-Alexa750, APC-eFluor780, APC-Vio770, Zombie NIR, L/Dfix NIR|
|* L/Dfix = Live/Dead Fixable|