Symphony A and B

Contact: Diane.Trotta@yale.edu or call (203)785-7958

The Facility has two identically configured BD Symphonies. Each is a six laser system capable of detecting in 33 different channels. Please see the configuration below for details and view Symphony resource page. Another way of viewing the configuration is using Fluorofinder web site, a useful panel building tool listing reagents from multiple companies. You can go to fluorofinder.com and follow Tools--> Flow Cytometry Panel Builder-->> Select your Instrument (after choosing Yale and School of Medicine FACS Facility from the drop-down menu).

The Symphonies are equipped with a 561 nm Yellow-green laser; this is different from the LSRIIs and Fortessas which have 532 nm green lasers. You should expect your PE and PE-tandem dyes to appear brighter than they did before. Less antibody might be required to avoid extreme adjustments on the sensitive detectors of this model.

Some fluorescent proteins that were barely detectable with a 532 nm laser will appear significantly brighter with 561 excitation. mCherry, mKate, and mRaspberry will perform much better with this laser line.

The Facility offers remote Symphony training for experienced LSRII/Fortessa users. Whether you've trained on the instrument before the pandemic and are looking for a refresher, or you've just decided you want to start using our sensitive 6 laser cytometers, our training video will raise your confidence to use the Symphony.

Please contact Diane.Trotta@yale.edu for information about Symphony training.

Both Symphonys are now available 24/7 once you are an approved, trained user.

1) Symphony A in TAC S613

2) Symphony B in Suite 203B, Room 2320H at 300 George St. This cytometer shares the room with a FACSAria sorter,

UV Laser-355nm-60mW
PMT	Bandpass	Long Pass	Fluorochrome	Other Optimal Fluorochromes
A	820/60	770	BUV805
B	740/35	690	BUV737
C	660/20	635	BUV661	Hoechst red
D	610/20	600	BUV615
E	565/20	550	BUV563
F	510/50	450	BUV496
G	450/50	410	DAPI	ZombieUV, L/DFix Blue, DyLight 350, Hoechst
H	379/28		BUV395
Violet Laser-405nm 200mW
PMT	Bandpass	Long Pass	Fluorochrome	Other Optimal Fluorochromes
A	780/60	770	BV786	Qdot800
B	750/30	710	BV750
C	710/30	690	BV711	Qdot705, SuperBright702
D	660/40	635	BV650	eFluor650NC, eVolve655, Superbright645
E	610/20	600	BV605	Qdot605, DyLight405LS, eFluor605NC, eVolve 605
F	586/15	550	BV570	Qdot585
G	525/50	505	BV510	L/DFix Aqua, V500, Al430, DyLight405LS, VioGreen
H	474/25	450	BV480
I	440/40	410	BV421	Pacific Blue, V450, Al405, CF405M, DyLight405, eFluor450, SuperBright436, VioBlue, Zombie Violet, L/DFixViolet
Blue Laser- 488 nm 200 mW
PMT	Bandpass	Long Pass	Fluorochrome	Other Optimal Fluorochromes
A	787/42	770	BB790
B	750/30	730	BB750
C	695/20	690	BB700	PerCP-Cy5.5, BB700, PerCP-eFluor710, PerCP-Vio700
D	670/25	630	PerCP	BB660
E	630/30	600	BB630
F	515/25	505	FITC	GFP, Al488, CFSE, BB515, DyLight488, Zombie Green
G	488/10		SSC
Yellow Green Laser-561nm 150 mW
PMT	Bandpass	Long Pass	Fluorochrome	Other Optimal Fluorochromes
A	780/60	750	PE-Cy7	PE-Vio770
B	710/50	690	PE-Cy5.5
C	660/20	650	PE-Cy5	mPlum
D	610/20	600	PE-TxRed	mCherry, PE-Dazzle, PE-CF594, PE-Vio615, PE-eFluor610, DyLight594, Zombie Red, L/DFix Red
E	586/15		PE	DsRed, RFP, tdTomato
Red Laser-637nm 140 mW
PMT	Bandpass	Long Pass	Fluorochrome	Other Optimal Fluorochromes
A	780/60	750	APC-Cy7	APC-Fire, APC-Al750, APC-eFluor 780, APC-Vio770, ZombieNIR, L/DfixNIR
B	710/25	685	Al700	Al680
C	660/20	650	APC	Al647, DyLight633, L/Dfix Far Red
NIR Laser - 780nm 100mW
PMT	Bandpass	LongPass	Fluorochrome	Other Optimal Fluorochromes
A	820/60	770LP	Al790
B	885/40	860LP	PromoFluor840
C	no optics