Pallavi Singh
Associate Research ScientistDownloadHi-Res Photo
About
Titles
Associate Research Scientist
Biography
Experienced Research Scientist with a demonstrated history of working in the field of infectious diseases including mosquito-borne (human malaria) and tick-borne (human babesiosis) diseases.
Last Updated on March 14, 2025.
Departments & Organizations
- Internal Medicine
Education & Training
- PhD
- Institut Pasteur, Paris (France), Parasite and Insect Vectors (2017)
- MSc
- University of Delhi, South Campus, Genetics (2012)
Research
Overview
Medical Research Interests
Babesiosis; Disease Transmission, Infectious; Host-Parasite Interactions; Malaria; Protozoan Infections; Tick-Borne Diseases; Vector Borne Diseases
ORCID
0000-0003-2318-5960
Research at a Glance
Yale Co-Authors
Frequent collaborators of Pallavi Singh's published research.
Publications Timeline
A big-picture view of Pallavi Singh's research output by year.
Research Interests
Research topics Pallavi Singh is interested in exploring.
Choukri Ben Mamoun, PhD
Pratap Vydyam, PhD
Albert Ko, MD
Chantal Vogels, PhD
Dennis G Moledina, MD, PhD, FASN
Jeffrey Testani, MD, MTR
17Publications
285Citations
Babesiosis
Host-Parasite Interactions
Malaria
Publications
Featured Publications
Babesia duncani multi-omics identifies virulence factors and drug targets
Singh P, Lonardi S, Liang Q, Vydyam P, Khabirova E, Fang T, Gihaz S, Thekkiniath J, Munshi M, Abel S, Ciampossin L, Batugedara G, Gupta M, Lu X, Lenz T, Chakravarty S, Cornillot E, Hu Y, Ma W, Gonzalez L, Sánchez S, Estrada K, Sánchez-Flores A, Montero E, Harb O, Le Roch K, Mamoun C. Babesia duncani multi-omics identifies virulence factors and drug targets. Nature Microbiology 2023, 8: 845-859. PMID: 37055610, PMCID: PMC10159843, DOI: 10.1038/s41564-023-01360-8.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsDrug targetsVirulence factorsCandidate virulence factorsRNA-seq dataIntraerythrocytic life cycleAttractive drug targetB. duncaniNuclear genomeGenome annotationApicomplexan parasitesApicomplexan pathogensEpigenetic profilesEpigenetic analysisParasite metabolismMalaria-like diseaseHuman erythrocytesLife cycle stagesBabesia speciesGenomeMetabolic requirementsCycle stagesLife cycleBiologySmall moleculesPotent inhibitorRole of a patatin-like phospholipase in Plasmodium falciparum gametogenesis and malaria transmission
Singh P, Alaganan A, More K, Lorthiois A, Thiberge S, Gorgette O, Guillotte Blisnick M, Guglielmini J, Aguilera S, Touqui L, Singh S, Chitnis C. Role of a patatin-like phospholipase in Plasmodium falciparum gametogenesis and malaria transmission. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 17498-17508. PMID: 31413195, PMCID: PMC6717283, DOI: 10.1073/pnas.1900266116.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsFemale gametesPerforin-like proteinBlood-stage growthIngestion of gametocytesProcess of gametogenesisPatatin-like phospholipaseGamete egressDiverse organismsMembrane remodelingIntracellular vesiclesVesicle secretionVesicular dischargeMale gametesOocyst formationPeripheral membraneGametocyte developmentGametogenesisExtensive membraneConditional deletionGametesBlood mealDevelopment of drugsDeletionPotential targetGenes
2025
Spermidine is a key polyamine required by intracellular parasites for survival within host erythrocytes
Singh P, Choi J, Cornillot E, Mamoun C. Spermidine is a key polyamine required by intracellular parasites for survival within host erythrocytes. Science Advances 2025, 11: eadv2397. PMID: 40531988, PMCID: PMC12175890, DOI: 10.1126/sciadv.adv2397.Peer-Reviewed Original ResearchAltmetricMeSH Keywords and ConceptsConceptsSpermidine biosynthesisIntracellular parasitesRegulate protein translationOxidative stress defenseDe novo synthesisProduction of reactive oxygen speciesTranslational regulationIncreased production of reactive oxygen speciesMolecular functionsProtein translationStress defensePlasmodium falciparum</i>Evolutionary adaptationReactive oxygen speciesAgent of human babesiosisIntraerythrocytic developmentBiosynthesisOxygen speciesHost erythrocytesTherapeutic targetA fluorescence-based assay for measuring aminopropyltransferase activity
https://doi.org/10.1016/bs.mie.2025.01.067ChaptersA fluorescence-based assay for measuring aminopropyltransferase activity
Singh P, Choi J, Ben Mamoun C. A fluorescence-based assay for measuring aminopropyltransferase activity. Methods In Enzymology 2025, 715: 363-388. PMID: 40382149, DOI: 10.1016/bs.mie.2025.01.067.Peer-Reviewed Original ResearchAltmetricMeSH Keywords and ConceptsConceptsAminopropyl transferaseFluorescence-based assaySpermine synthase activityHigh-throughput chemical screeningSaccharomyces cerevisiaeFormation of spermidinePrincipal polyaminesCellular processesHigh-throughput screeningCellular functionsSubstrate analogsSpermidine synthaseDrug discovery effortsPolyamine biosynthesisLiving cellsChemical screeningEnzymeNeurodegenerative disordersPolycationic alkylaminesSynthase activityDiscovery effortsNovel inhibitorsAPT activityChemical librariesInfectious diseases
2024
A fluorescence-based assay for measuring polyamine biosynthesis aminopropyl transferase–mediated catalysis
Singh P, Choi J, Wang W, Lam T, Lechner P, Vanderwal C, Pou S, Nilsen A, Mamoun C. A fluorescence-based assay for measuring polyamine biosynthesis aminopropyl transferase–mediated catalysis. Journal Of Biological Chemistry 2024, 300: 107832. PMID: 39342998, PMCID: PMC11541840, DOI: 10.1016/j.jbc.2024.107832.Peer-Reviewed Original ResearchCitationsAltmetricConceptsAminopropyl transferaseFluorescence-based assayLack of high-throughput assaysHigh-throughput screeningCarbon chain lengthChemical librariesMass spectrometryChain lengthHigh-throughput assayDrug discoveryMass spectrometry analysisSaccharomyces cerevisiaeThin-layer chromatographyFluorescence intensityCellular functionsSpectrometry analysisPolycationic moleculesFluorescent conjugatesIsoindoleAPT activityCatalysisAssayBenzeneAdductsEnzymeInsights into the evolution, virulence and speciation of Babesia MO1 and Babesia divergens through multiomics analyses
Singh P, Vydyam P, Fang T, Estrada K, Gonzalez L, Grande R, Kumar M, Chakravarty S, Berry V, Ranwez V, Carcy B, Depoix D, Sánchez S, Cornillot E, Abel S, Ciampossin L, Lenz T, Harb O, Sanchez-Flores A, Montero E, Le Roch K, Lonardi S, Mamoun C. Insights into the evolution, virulence and speciation of Babesia MO1 and Babesia divergens through multiomics analyses. Emerging Microbes & Infections 2024, 13: 2386136. PMID: 39148308, PMCID: PMC11370697, DOI: 10.1080/22221751.2024.2386136.Peer-Reviewed Original ResearchCitationsAltmetricConceptsLeveraging genomic informationHuman babesiosisTick-borne diseasesDiseases of significanceCases of human babesiosisGenomic divergenceGenome sequenceGenomic informationMultigene familyGene functionBabesia divergensMammalian hostsAnimal healthMultiomics analysisZoonotic pathogensBabesiosisProtozoan parasitesVirulent strainsPathogensVertebrate hostsEnvironmental changesVirulenceReplication rateAntiparasitic drugsParasitesVitamin B5 metabolism is essential for vacuolar and mitochondrial functions and drug detoxification in fungi
Choi J, Gihaz S, Munshi M, Singh P, Vydyam P, Hamel P, Adams E, Sun X, Khalimonchuk O, Fuller K, Ben Mamoun C. Vitamin B5 metabolism is essential for vacuolar and mitochondrial functions and drug detoxification in fungi. Communications Biology 2024, 7: 894. PMID: 39043829, PMCID: PMC11266677, DOI: 10.1038/s42003-024-06595-7.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsSusceptibility of fungiRegulation of genesMetabolism of fatty acidsVacuolar morphologySaccharomyces cerevisiaeAcetyl-CoAEukaryotic pathogensGenetic evidenceGenetic regulationCellular processesAntifungal drugsCo-enzyme ADrug detoxificationAntifungal therapyDrug-resistant strainsFungal infectionsMitochondrial functionFungiAmino acidsAR-12Vitamin B5Synthase activityPathwayExcellent targetGlobal health threat
2022
Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti
Chand M, Choi J, Pal A, Singh P, Kumari V, Thekkiniath J, Gagnon J, Timalsina S, Gaur G, Williams S, Ledizet M, Mamoun C. Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti. Frontiers In Cellular And Infection Microbiology 2022, 12: 1039197. PMID: 36506011, PMCID: PMC9732259, DOI: 10.3389/fcimb.2022.1039197.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsMonoclonal antibodiesAntigen detection assaysMajor immunogenic determinantMost clinical casesReliable biomarkersAntibody combinationsImmunogenic determinantsIntraerythrocytic life cycleClinical casesUnique epitopesBabesia microtiEpitope profilingHuman babesiosisTick-borne diseasesSignificant riseSerological profilingPlasma samplesAntibodiesSerological characterizationProtozoan parasitePublic healthInfectionAntigenDiseaseMammalian hostsBabesia duncani in Culture and in Mouse (ICIM) Model for the Advancement of Babesia Biology, Pathogenesis, and Therapy.
Kumari V, Pal A, Singh P, Mamoun C. Babesia duncani in Culture and in Mouse (ICIM) Model for the Advancement of Babesia Biology, Pathogenesis, and Therapy. Bio-protocol 2022, 12 PMID: 36620533, PMCID: PMC9795036, DOI: 10.21769/bioprotoc.4549.Peer-Reviewed Original ResearchCitationsAltmetricConceptsMouse modelHuman red blood cellsRed blood cellsC3H/HeJ miceBlood cellsHuman babesiosisMalaria-like illnessB. duncaniImportant health impactsTick-borne diseaseBlood transfusionTick biteLethal infectionHeJ miceRare caseBabesia duncaniImmunocompromised miceAnimal modelsHuman infectionsParasitic diseasesBabesia microtiDiseaseElderly peopleIntraerythrocytic parasitesB. microti
News
News
- June 18, 2025
How Parasites—and a Bit of Serendipity—Led to a Powerful New Drug Discovery Tool: A Q&A with Choukri Ben Mamoun
- October 24, 2024
Further Babesiosis Biology, Pathogenicity Research Underway at Yale
- April 13, 2023
New Multi-Omics Study Sheds Light on Virulence and Vulnerability of a Babesia Parasite