M. Elizabeth Deerhake, MD, PhD
Cards
About
Research
Publications
2022
Dectin-1 signaling in neutrophils up-regulates PD-L1 and triggers ROS-mediated suppression of CD4+ T cells
Deerhake ME, Cardakli ED, Shinohara ML. Dectin-1 signaling in neutrophils up-regulates PD-L1 and triggers ROS-mediated suppression of CD4+ T cells. Journal Of Leukocyte Biology 2022, 112: 1413-1425. PMID: 36073780, PMCID: PMC9701158, DOI: 10.1002/jlb.3a0322-152rr.Peer-Reviewed Original ResearchConceptsPD-L1 expressionExperimental autoimmune encephalomyelitisPD-L1T cellsT cell-suppressive propertiesUp-regulating PD-L1T cell-driven inflammationImmune checkpoint factorsNeutrophil PD-L1Suppression of CD4T cell responsesProinflammatory cytokine productionT cell viabilityHost defense functionsAutoimmune encephalomyelitisDendritic cellsCytokine productionMHC-IINeutrophil responseCD4Disease severityCell responsesNeutrophilsFungal infectionsReactive oxygen speciesJournal Club: Neurofilament Light Chain Levels in Anti-NMDAR Encephalitis and Primary Psychiatric Psychosis
Deerhake M, Giarraputo J, Gupta M, Eckstein C. Journal Club: Neurofilament Light Chain Levels in Anti-NMDAR Encephalitis and Primary Psychiatric Psychosis. Neurology 2022, 99: 854-857. PMID: 36038273, PMCID: PMC9651450, DOI: 10.1212/wnl.0000000000201235.Peer-Reviewed Original ResearchConceptsNMDA receptor encephalitisAntibody testingNeurofilament light chain levelsAutoimmune brain disordersAnti-NMDAR encephalitisSubset of patientsPrimary psychiatric disordersLight chain levelsReceptor encephalitisLumbar punctureNMDA receptorsSerum testPsychiatric disordersBrain disordersClinical settingEncephalitisDisordersPatientsPuncturePsychosisCSFChain levelAntibodiesReceptorsThe AIM2 inflammasome is activated in astrocytes during the late phase of EAE
Barclay WE, Aggarwal N, Deerhake ME, Inoue M, Nonaka T, Nozaki K, Luzum NA, Miao EA, Shinohara ML. The AIM2 inflammasome is activated in astrocytes during the late phase of EAE. JCI Insight 2022, 7: e155563. PMID: 35451371, PMCID: PMC9089781, DOI: 10.1172/jci.insight.155563.Peer-Reviewed Original ResearchConceptsExperimental autoimmune encephalomyelitisCentral nervous systemInflammasome activationInflammasome-mediated inflammationRole of inflammasomesApoptosis-associated speck-like proteinIL-1β releaseAIM2 inflammasome activationSpeck-like proteinAutoimmune encephalomyelitisEffector cytokinesAutoimmune conditionsIL-18Multiple sclerosisIL-1βDisease peakInflammatory responseSpinal cordMelanoma 2Mouse modelAnimal modelsReporter miceNervous systemMyeloid cellsAIM2 inflammasome
2021
Secreted osteopontin from CD4+ T cells limits acute graft-versus-host disease
Aggarwal N, Deerhake ME, DiPalma D, Shahi SK, Gaggioli MR, Mangalam AK, Shinohara ML. Secreted osteopontin from CD4+ T cells limits acute graft-versus-host disease. Cell Reports 2021, 37: 110170. PMID: 34965439, PMCID: PMC8759344, DOI: 10.1016/j.celrep.2021.110170.Peer-Reviewed Original ResearchConceptsIntestinal epithelial cellsAcute GVHDHost diseaseMajor target organAcute graftBacteria AkkermansiaGastrointestinal pathologyMouse modelTarget organsFunction of osteopontinCD4Protective rolePotential biomarkersBeneficial effectsEpithelial cellsOPN isoformsMouse mutant modelsGVHDOsteopontinGraftCell deathDiseaseCellsMutant modelsAkkermansiaEmerging roles of Dectin-1 in noninfectious settings and in the CNS
Deerhake ME, Shinohara ML. Emerging roles of Dectin-1 in noninfectious settings and in the CNS. Trends In Immunology 2021, 42: 891-903. PMID: 34489167, PMCID: PMC8487984, DOI: 10.1016/j.it.2021.08.005.Peer-Reviewed Original ResearchConceptsC-type lectin receptorsImmune responseProinflammatory immune responseCentral nervous systemNew therapeutic approachesInnate immune responseMammalian myeloid cellsNeuroprotective responseSterile inflammationNeurologic disordersTherapeutic approachesNervous systemMyeloid cellsFungal infectionsLectin receptorsRecent studiesCurrent understandingResponseInflammationΒ-glucanInfectionSettingImmunologyReceptorsDectin-1 limits CNS autoimmunity through a non-canonical pathway
Deerhake M, Danzaki K, Inoue M, Cardakli E, Nonaka T, Aggarwal N, Barclay W, Ji R, Shinohara M. Dectin-1 limits CNS autoimmunity through a non-canonical pathway. The Journal Of Immunology 2021, 206: 96.07-96.07. DOI: 10.4049/jimmunol.206.supp.96.07.Peer-Reviewed Original ResearchExperimental autoimmune encephalomyelitisC-type lectin receptorsCentral nervous systemMultiple sclerosisInnate immunityDectin-1 pathwayCNS autoimmunityEAE severityAutoimmune encephalomyelitisNeuroprotective cytokineNeuroprotective functionNeurologic disordersPathologic roleImmune responseAnimal modelsNervous systemMyeloid cellsOSM receptorNon-canonical pathwayUpregulated expressionOncostatin MLectin receptorsAutoimmunityRNA-seq profilingProtective aspectsSingle-Cell Transcriptional Heterogeneity of Neutrophils During Acute Pulmonary Cryptococcus neoformans Infection
Deerhake ME, Reyes EY, Xu-Vanpala S, Shinohara ML. Single-Cell Transcriptional Heterogeneity of Neutrophils During Acute Pulmonary Cryptococcus neoformans Infection. Frontiers In Immunology 2021, 12: 670574. PMID: 33995406, PMCID: PMC8116703, DOI: 10.3389/fimmu.2021.670574.Peer-Reviewed Original ResearchConceptsPulmonary Cryptococcus neoformans infectionDistinct neutrophil subsetsCryptococcus neoformans infectionRole of neutrophilsLigand-receptor analysisCytokine gene expressionFirst-line defensePulmonary cryptococcosisNeoformans infectionDendritic cellsNeutrophil subsetsNeutrophil heterogeneityNeutrophil functionImmune cellsCell transcriptional heterogeneityAlveolar macrophagesNeutrophilsFungal infectionsCryptococcus neoformansInfectionSingle-cell transcriptional analysisGene expressionGenerate ROSOxidative stress signatureTranscriptional heterogeneityDectin-1 limits autoimmune neuroinflammation and promotes myeloid cell-astrocyte crosstalk via Card9-independent expression of Oncostatin M
Deerhake ME, Danzaki K, Inoue M, Cardakli ED, Nonaka T, Aggarwal N, Barclay WE, Ji RR, Shinohara ML. Dectin-1 limits autoimmune neuroinflammation and promotes myeloid cell-astrocyte crosstalk via Card9-independent expression of Oncostatin M. Immunity 2021, 54: 484-498.e8. PMID: 33581044, PMCID: PMC7956124, DOI: 10.1016/j.immuni.2021.01.004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAstrocytesBrainCARD Signaling Adaptor ProteinsCell CommunicationCells, CulturedDisease Models, AnimalEncephalomyelitis, Autoimmune, ExperimentalGalectinsGene Expression RegulationLectins, C-TypeMice, Inbred C57BLMice, KnockoutMultiple SclerosisMyelin-Oligodendrocyte GlycoproteinMyeloid CellsNeurogenic InflammationOncostatin MOncostatin M Receptor beta SubunitPeptide FragmentsReceptors, MitogenSignal TransductionConceptsExperimental autoimmune encephalomyelitisC-type lectin receptorsCentral nervous systemAutoimmune neuroinflammationOncostatin MPro-resolution functionHeat-killed mycobacteriaDectin-1 pathwayDectin-1 ligandsPotential therapeutic targetEAE severityAutoimmune encephalomyelitisNeuroprotective moleculesNeurologic disordersPathologic roleGalectin-9Therapeutic targetTranscription factor NFATNervous systemMyeloid cellsInnate immunityOSM receptorLectin receptorsEnhanced gene expressionNeuroinflammation
2020
Functional heterogeneity of alveolar macrophage population based on expression of CXCL2
Xu-Vanpala S, Deerhake ME, Wheaton JD, Parker ME, Juvvadi PR, MacIver N, Ciofani M, Shinohara ML. Functional heterogeneity of alveolar macrophage population based on expression of CXCL2. Science Immunology 2020, 5 PMID: 32769172, PMCID: PMC7717592, DOI: 10.1126/sciimmunol.aba7350.Peer-Reviewed Original ResearchConceptsPulmonary fungal infectionAlveolar macrophagesFungal infectionsAM subpopulationsPharmacological intervention targetPro-inflammatory moleculesLung-resident macrophagesAnti-inflammatory moleculesExpression of CXCL2Strong inflammatory responseAlveolar macrophage populationComplement component 1qDistinct metabolic profilesC1q expressionInterleukin-10Inflammatory responseSentinel cellsPhagocytic capacityMacrophage populationsCXCL2InfectionMetabolic profileIntervention targetsSubpopulationsAM population
2019
Pattern Recognition Receptors in Multiple Sclerosis and Its Animal Models
Deerhake ME, Biswas DD, Barclay WE, Shinohara ML. Pattern Recognition Receptors in Multiple Sclerosis and Its Animal Models. Frontiers In Immunology 2019, 10: 2644. PMID: 31781124, PMCID: PMC6861384, DOI: 10.3389/fimmu.2019.02644.Peer-Reviewed Original ResearchConceptsExperimental autoimmune encephalomyelitisPattern recognition receptorsMultiple sclerosisCNS autoimmunityAnimal modelsRecognition receptorsIts Animal ModelsNon-immune cellsInnate immune responseProtective functionFunction of PRRsPRR responsesAutoimmune encephalomyelitisImmune responseEndogenous ligandAutoimmunitySclerosisFurther investigationReceptorsDistinct triggersEncephalomyelitisResponse