2020
Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets
Vogels CBF, Brito AF, Wyllie AL, Fauver JR, Ott IM, Kalinich CC, Petrone ME, Casanovas-Massana A, Catherine Muenker M, Moore AJ, Klein J, Lu P, Lu-Culligan A, Jiang X, Kim DJ, Kudo E, Mao T, Moriyama M, Oh JE, Park A, Silva J, Song E, Takahashi T, Taura M, Tokuyama M, Venkataraman A, Weizman OE, Wong P, Yang Y, Cheemarla NR, White EB, Lapidus S, Earnest R, Geng B, Vijayakumar P, Odio C, Fournier J, Bermejo S, Farhadian S, Dela Cruz CS, Iwasaki A, Ko AI, Landry ML, Foxman EF, Grubaugh ND. Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets. Nature Microbiology 2020, 5: 1299-1305. PMID: 32651556, PMCID: PMC9241364, DOI: 10.1038/s41564-020-0761-6.Peer-Reviewed Original ResearchMeSH KeywordsBetacoronavirusClinical Laboratory TechniquesCoronavirus InfectionsCOVID-19COVID-19 TestingGenetic VariationGenome, ViralHumansMolecular Probe TechniquesPandemicsPneumonia, ViralReverse Transcriptase Polymerase Chain ReactionRNARNA ProbesRNA, ViralSARS-CoV-2Sensitivity and SpecificityConceptsSARS-CoV-2SARS-CoV-2 RTSevere acute respiratory syndrome coronavirusAcute respiratory syndrome coronavirusViral RNA copiesPublic health laboratoriesPublic health interventionsReverse transcription-PCR assaySARS-CoV-2 diagnostic testingDiagnostic assaysTranscription-PCR assaySARS-CoV-2 evolutionQuantitative reverse transcription-PCR assaysRapid diagnostic assaysHealth laboratoriesHealth interventionsDiagnostic testingRNA copiesPrimer-probe setsAssaysLow sensitivityCritical needAnalytical sensitivity
2011
Diagnostic tests for influenza infection
Landry ML. Diagnostic tests for influenza infection. Current Opinion In Pediatrics 2011, 23: 91-97. PMID: 21150446, DOI: 10.1097/mop.0b013e328341ebd9.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, ViralCell Culture TechniquesChildFalse Negative ReactionsFluorescent Antibody TechniqueHumansInfluenza A Virus, H1N1 SubtypeInfluenza, HumanReagent Kits, DiagnosticReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityConceptsMolecular testingMultiplex molecular testRapid antigen testInfluenza infectionViral loadAntigen testPatient groupSeasonal influenzaImmunofluorescence testDiagnostic test performanceAntigenic changesNext pandemicRapid cultureDiagnostic testsMolecular testsMarked variabilityNegative resultsSame testDiagnostic methods
2009
Cytospin-Enhanced Immunofluorescence and Impact of Sample Quality on Detection of Novel Swine Origin (H1N1) Influenza Virus
Landry ML, Ferguson D. Cytospin-Enhanced Immunofluorescence and Impact of Sample Quality on Detection of Novel Swine Origin (H1N1) Influenza Virus. Journal Of Clinical Microbiology 2009, 48: 957-959. PMID: 20042632, PMCID: PMC2832425, DOI: 10.1128/jcm.01678-09.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAnimalsChildChild, PreschoolClinical Laboratory TechniquesFluorescent Antibody Technique, DirectHumansInfantInfant, NewbornInfluenza A Virus, H1N1 SubtypeInfluenza, HumanNasopharynxReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityVirologyYoung AdultConceptsNovel swine-origin influenza virusesSwine-origin influenza virusImmunofluorescence antibody testingReal-time reverse transcriptase PCRCycle threshold valuesPCR-positive resultsPediatric patientsCDC assayAntibody testingNasopharyngeal swabsReverse transcriptase-PCRSwine influenzaInfluenza virusTranscriptase-PCRPositive samplesPatientsPositive resultsInfluenzaSwabsCytospinsRhinoviruses Are a Major Cause of Wheezing and Hospitalization in Children Less Than 2 Years of Age
Piotrowska Z, Vázquez M, Shapiro ED, Weibel C, Ferguson D, Landry ML, Kahn JS. Rhinoviruses Are a Major Cause of Wheezing and Hospitalization in Children Less Than 2 Years of Age. The Pediatric Infectious Disease Journal 2009, 28: 25-29. PMID: 19057454, PMCID: PMC4639321, DOI: 10.1097/inf.0b013e3181861da0.Peer-Reviewed Original ResearchMeSH KeywordsData Interpretation, StatisticalHospitalizationHumansInfantMolecular EpidemiologyPhylogenyPicornaviridae InfectionsRespiratory SoundsRespiratory Tract InfectionsReverse Transcriptase Polymerase Chain ReactionRhinovirusSequence Analysis, RNAConceptsRespiratory syncytial virusHRV-positive childrenHuman rhinovirusSyncytial virusAge groupsDiagnostic laboratoriesRespiratory tract diseaseMajor respiratory pathogenRate of infectionYears of ageSimilar age groupAsymptomatic childrenRespiratory specimenAsymptomatic groupRespiratory infectionsHRV infectionHospitalization ratesPolymerase chain reactionTract diseaseRespiratory pathogensHRV isolatesMajor causeStudy periodSymptomsInfection
2008
Real-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients
Landry ML, Cohen S, Ferguson D. Real-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients. Journal Of Clinical Virology 2008, 43: 148-151. PMID: 18639488, PMCID: PMC7173065, DOI: 10.1016/j.jcv.2008.06.006.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overAntibodies, MonoclonalChildChild, PreschoolFluorescent Antibody Technique, DirectHospitalizationHumansImmunoassayInfantInfluenza A virusInfluenza B virusInfluenza, HumanMiddle AgedNasopharynxReagent Kits, DiagnosticReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityTaq PolymeraseTime FactorsVirus CultivationYoung AdultConceptsHospitalized patientsTaqMan RT-PCRRT-PCRInfluenza ARapid flu testsDiagnosis of influenzaInfluenza-positive samplesDetection of influenzaReal-time RT-PCRInfluenza BInfluenza diagnosisPatient managementReal-time PCRMore accurate testsPatientsBinaxTrue positive samplesRapid testClinical samplesPositive samplesFurther studiesHospitalAccurate testInfluenzaDiagnosis
2006
Coronavirus HKU1 Infection in the United States
Esper F, Weibel C, Ferguson D, Landry ML, Kahn JS. Coronavirus HKU1 Infection in the United States. Emerging Infectious Diseases 2006, 12: 775-779. PMID: 16704837, PMCID: PMC3374449, DOI: 10.3201/eid1205.051316.Peer-Reviewed Original ResearchMeSH KeywordsCoronavirusCoronavirus InfectionsFemaleHospitalizationHumansInfantMaleRespiratory Tract InfectionsReverse Transcriptase Polymerase Chain ReactionRNA, ViralUnited StatesConceptsHCoV-HKU1Respiratory specimensLower respiratory tract infectionsLower respiratory tract diseaseHCoV-HKU1 infectionRespiratory tract infectionsRespiratory syncytial virusRespiratory tract diseaseReverse transcription-polymerase chain reactionNew human coronavirusYears of ageTranscription-polymerase chain reactionTract infectionsSyncytial virusPositive childrenParainfluenza virusTract diseaseRespiratory tractHuman coronavirusesPositive specimensInfluenza virusInfectionChain reactionChildrenVirusHuman metapneumovirus and exacerbations of chronic obstructive pulmonary disease
Martinello RA, Esper F, Weibel C, Ferguson D, Landry ML, Kahn JS. Human metapneumovirus and exacerbations of chronic obstructive pulmonary disease. Journal Of Infection 2006, 53: 248-254. PMID: 16412516, PMCID: PMC7112509, DOI: 10.1016/j.jinf.2005.11.010.Peer-Reviewed Original ResearchMeSH KeywordsAgedAged, 80 and overConnecticutFemaleHospitalizationHospitals, TeachingHospitals, UrbanHumansMaleMetapneumovirusMiddle AgedParamyxoviridae InfectionsPhylogenyPulmonary Disease, Chronic ObstructiveReverse Transcriptase Polymerase Chain ReactionConceptsExacerbation of COPDChronic obstructive pulmonary diseaseObstructive pulmonary diseaseRespiratory syncytial virusHuman metapneumovirusPulmonary diseaseSyncytial virusObservational cohort studyRespiratory tract infectionsParainfluenza type 3Urban teaching hospitalCOPD patientsCohort studyTract infectionsParainfluenza 1HMPV genotypesRespiratory virusesNasopharyngeal specimensViral cultureEnrollment criteriaInfluenza ATeaching hospitalDirect immunofluorescenceExacerbationPatients
2005
Association between a Novel Human Coronavirus and Kawasaki Disease
Esper F, Shapiro ED, Weibel C, Ferguson D, Landry ML, Kahn JS. Association between a Novel Human Coronavirus and Kawasaki Disease. The Journal Of Infectious Diseases 2005, 191: 499-502. PMID: 15655771, PMCID: PMC7199489, DOI: 10.1086/428291.Peer-Reviewed Original ResearchMeSH KeywordsCase-Control StudiesChild, PreschoolCoronavirusCoronavirus InfectionsFemaleHumansInfantMaleMucocutaneous Lymph Node SyndromeRespiratory SystemReverse Transcriptase Polymerase Chain ReactionConceptsKawasaki diseaseNovel human coronavirusRespiratory secretionsHuman coronavirusesClassic Kawasaki diseaseNew Haven coronavirusCase-control studyReverse transcriptase-polymerase chain reactionSystemic vasculitisControl subjectsPolymerase chain reactionPossible associationDiseaseChain reactionInfectionCoronavirusSecretionAssociationVasculitisInfantsEtiology
2004
A 1-Year Experience with Human Metapneumovirus in Children Aged <5 Years
Esper F, Martinello RA, Boucher D, Weibel C, Ferguson D, Landry ML, Kahn JS. A 1-Year Experience with Human Metapneumovirus in Children Aged <5 Years. The Journal Of Infectious Diseases 2004, 189: 1388-1396. PMID: 15073675, PMCID: PMC7109939, DOI: 10.1086/382482.Peer-Reviewed Original ResearchMeSH KeywordsAustraliaCanadaChild, PreschoolConnecticutFemaleHumansInfantInfant, NewbornMaleMetapneumovirusNetherlandsParamyxoviridae InfectionsPhylogenyRespiratory Tract InfectionsReverse Transcriptase Polymerase Chain ReactionRNA, ViralSeasonsSequence AlignmentSequence Analysis, DNAConceptsHuman metapneumovirusPresence of hMPVStudy periodHMPV-positive childrenRespiratory syncytial virusParainfluenza virus 1Respiratory tract diseaseReverse transcription-polymerase chain reactionDirect fluorescent antibody testFluorescent antibody testSyncytial virusRespiratory specimensPolymerase chain reactionChildren AgedCommon findingInfluenza ARespiratory pathogensChest wallAntibody testVirus 1Chain reactionNegative resultsMetapneumovirusSignificant proportionChildren
2003
Comparison of the NucliSens Basic Kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR Assays for Rapid Detection of Enterovirus RNA in Clinical Specimens
Landry ML, Garner R, Ferguson D. Comparison of the NucliSens Basic Kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR Assays for Rapid Detection of Enterovirus RNA in Clinical Specimens. Journal Of Clinical Microbiology 2003, 41: 5006-5010. PMID: 14605131, PMCID: PMC262477, DOI: 10.1128/jcm.41.11.5006-5010.2003.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceConsensus SequenceEnterovirusEnterovirus InfectionsGene AmplificationHumansPolymerase Chain ReactionReagent Kits, DiagnosticReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionRNA, ViralSensitivity and SpecificityConceptsNucleic acid sequence-based amplificationNucliSens Basic KitRT-PCREnterovirus RNABasic KitEnterovirus-positive samplesReverse transcription-PCR assayTranscription-PCR assayNasopharyngeal samplesRT-PCR kitVirus isolationClinical specimensTranscription-PCRPositive samplesComparable sensitivityKitMolecular methodsEnterovirusesComparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus
Habib-Bein NF, Beckwith WH, Mayo D, Landry ML. Comparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus. Journal Of Clinical Microbiology 2003, 41: 3597-3601. PMID: 12904361, PMCID: PMC179819, DOI: 10.1128/jcm.41.8.3597-3601.2003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsConnecticutFluorescent Antibody Technique, DirectHumansInfluenza A virusInfluenza, HumanMacaca mulattaNasopharynxPolymerase Chain ReactionPublic HealthReverse Transcriptase Polymerase Chain ReactionConceptsVirus-positive specimensDirect immunofluorescence antibody stainingPublic health laboratoriesHealth laboratoriesRT-PCRMedical CenterRapid diagnosisYale-New Haven HospitalReal-time RT-PCRResults of cultureRhesus monkey kidney cell culturesImmunofluorescence antibody stainingReal-time reverse transcriptionInfluenza A virusesMonkey kidney cell culturesConventional RT-PCR analysisReal-time RT-PCR systemRespiratory specimensInfluenza APatient managementDirect immunofluorescenceCell culturesKidney cell culturesRapid turnaround timeA virus