2025
Cross-modal sensory compensation increases mosquito attraction to humans
Morita T, Lyn N, von Heynitz R, Goldman O, Sorrells T, DeGennaro M, Matthews B, Houri-Zeevi L, Vosshall L. Cross-modal sensory compensation increases mosquito attraction to humans. Science Advances 2025, 11: eadn5758. PMID: 39742477, PMCID: PMC11691641, DOI: 10.1126/sciadv.adn5758.Peer-Reviewed Original Research
2024
CRISPR-Cas9-based approaches for genetic analysis and epistatic interaction studies in Coxiella burnetii
Steiner S, Roy C. CRISPR-Cas9-based approaches for genetic analysis and epistatic interaction studies in Coxiella burnetii. MSphere 2024, 9: e00523-24. PMID: 39560384, PMCID: PMC11656778, DOI: 10.1128/msphere.00523-24.Peer-Reviewed Original ResearchIntracellular replicationHost cellsDouble mutantGenetic analysisDot/Icm type IVB secretion systemType IVB secretion systemConstruction of double mutantsLoss-of-function mutantsIntracellular replication defectsLysosome-derived vacuoleIVB secretion systemObligate intracellular bacterial pathogenRandom transposon mutagenesisTransposon insertion mutantsCRISPR-Cas9-based technologiesIntracellular bacterial pathogensLoss-of-function mutationsMammalian host cellsIntroduction of premature stop codonsPremature stop codonSilence gene expressionTargeted gene disruptionBase editing approachesEffector mutantsSecretion systemLive imaging of Fibronectin 1a-mNeonGreen and Fibronectin 1b-mCherry knock-in alleles during early zebrafish development
Jülich D, Holley S. Live imaging of Fibronectin 1a-mNeonGreen and Fibronectin 1b-mCherry knock-in alleles during early zebrafish development. Cells And Development 2024, 177: 203900. PMID: 38218338, PMCID: PMC10947920, DOI: 10.1016/j.cdev.2024.203900.Peer-Reviewed Original ResearchKnock-in alleleECM fibersLoss of function phenotypesGenetic complementation experimentsAdhesion receptor familyComplementation experimentsDouble mutantLive in vivo imagingExtracellular matrix glycoproteinFibronectin matrixPosterior hindgutPresomitic mesodermZebrafish developmentExpression patternsMRNA injectionIntegrinLive imagingCross-regulationAllelesFibronectinReceptor familyParaxial mesodermCo-localizationExtracellular matrixMutants
2023
Arabidopsis Tubby domain‐containing F‐box proteins positively regulate immunity by modulating PI4Kβ protein levels
Devendrakumar K, Copeland C, Adamchek C, Zhong X, Huang X, Gendron J, Li X. Arabidopsis Tubby domain‐containing F‐box proteins positively regulate immunity by modulating PI4Kβ protein levels. New Phytologist 2023, 240: 354-371. PMID: 37571862, PMCID: PMC11114105, DOI: 10.1111/nph.19187.Peer-Reviewed Original ResearchConceptsTubby-like proteinsN-terminal F-box domainF-box E3 ligase complexesImmunoprecipitation-mass spectrometry analysisArabidopsis Immune ResponsesPlant immune responsesProtein levelsDomain-containing proteinsF-box domainF-box proteinsE3 ligase complexProteasome-dependent mannerRedundant homologsOverexpression linesTubby domainUbiquitination substratesSkp1-CullinTLP functionsLigase complexTubby proteinBiosynthesis enzymesDouble mutantMass spectrometry analysisProtein 6Novel mechanism
2021
H3.1K27me1 maintains transcriptional silencing and genome stability by preventing GCN5-mediated histone acetylation
Dong J, LeBlanc C, Poulet A, Mermaz B, Villarino G, Webb KM, Joly V, Mendez J, Voigt P, Jacob Y. H3.1K27me1 maintains transcriptional silencing and genome stability by preventing GCN5-mediated histone acetylation. The Plant Cell 2021, 33: 961-979. PMID: 33793815, PMCID: PMC8226292, DOI: 10.1093/plcell/koaa027.Peer-Reviewed Original ResearchConceptsGenome stabilityGenomic instabilityHistone acetylationSAGA-like complexesMultiple lysine residuesArabidopsis GCN5ARABIDOPSIS TRITHORAXArabidopsis thalianaTranscriptional silencingHeterochromatin defectsDouble mutantDNA replicationEpigenetic mechanismsGCN5Molecular roleEssential functionsDiverse rolesMolecular mechanismsLysine residuesProtein 5AcetylationMutantsPlantsADA2bATXR6
2020
Three STIGMA AND STYLE STYLISTs Pattern the Fine Architectures of Apical Gynoecium and Are Critical for Male Gametophyte-Pistil Interaction
Li W, Huang X, Zou J, Wu J, Jiao H, Peng X, Sun M. Three STIGMA AND STYLE STYLISTs Pattern the Fine Architectures of Apical Gynoecium and Are Critical for Male Gametophyte-Pistil Interaction. Current Biology 2020, 30: 4780-4788.e5. PMID: 33007250, DOI: 10.1016/j.cub.2020.09.006.Peer-Reviewed Original ResearchConceptsApical gynoeciumSexual reproductionPollen tubesPlant sexual reproductionTransmitting tract tissueEfficient sexual reproductionSame regulatory pathwayFusion of carpelsNovel molecular mechanismPollen tube growthGene familyPlant fertilityDouble mutantTranscription factorsTransmitting tractRegulatory pathwaysMolecular mechanismsTube growthGynoeciumCell typesFine architectureCell expansionCritical roleReproductionTissue distribution
2019
Reengineering a tRNA Methyltransferase To Covalently Capture New RNA Substrates
Smith TS, Zoltek MA, Simon MD. Reengineering a tRNA Methyltransferase To Covalently Capture New RNA Substrates. Journal Of The American Chemical Society 2019, 141: 17460-17465. PMID: 31626536, DOI: 10.1021/jacs.9b08529.Peer-Reviewed Original ResearchConceptsSubstrate specificityRNA substratesSubstrate RNARNA substrate specificityWild-type proteinCovalent RNA modificationsHigh-throughput sequencing assaysDouble mutantRNA modificationsTriple mutantSubstrate mutantsTRNA methyltransferaseProtein engineeringRNA transcriptsMutantsRational engineeringRNASubstrate screeningTRMASequencing assaysMethyltransferaseEnzymeProteinPowerful toolSpecificitySerotonin and neuropeptides are both released by the HSN command neuron to initiate C. elegans egg laying
Brewer JC, Olson AC, Collins KM, Koelle MR. Serotonin and neuropeptides are both released by the HSN command neuron to initiate C. elegans egg laying. PLOS Genetics 2019, 15: e1007896. PMID: 30677018, PMCID: PMC6363226, DOI: 10.1371/journal.pgen.1007896.Peer-Reviewed Original ResearchConceptsHermaphrodite-specific neuronsEgg-laying defectsNLP-3C. elegansEgg-laying musclesEgg-laying circuitDirect postsynaptic targetsEgg-laying behaviorSerotonergic Hermaphrodite Specific NeuronsMuscle cellsSmall molecule neurotransmittersNull mutantsHSN neuronsDouble mutantSingle mutantsMutant animalsSerotonergic neuronsWild-type animalsSevere defectsMutantsElegansNeuropeptide substance PMammalian brainEggsSpecific neurons
2016
A pivot mutation impedes reverse evolution across an adaptive landscape for drug resistance in Plasmodium vivax
Ogbunugafor C, Hartl D. A pivot mutation impedes reverse evolution across an adaptive landscape for drug resistance in Plasmodium vivax. Malaria Journal 2016, 15: 40. PMID: 26809718, PMCID: PMC4727274, DOI: 10.1186/s12936-016-1090-3.Peer-Reviewed Original ResearchConceptsEpistatic interactionsAdaptive landscapeCompensatory mutationsFitness effects of individual mutationsDynamics of evolutionResistance patternsGenome sequencing effortsEffects of individual mutationsWild type ancestorsPopulations of pathogensLocal fitness peaksPlasmodium vivaxResistance proteinReverse evolutionSequencing effortsAncestral genotypesDouble mutantResistance-associated markersQuadruple mutantPolymorphic populationsGenomic signaturesIndividual mutationsSingle mutationsSusceptible phenotypeWild type
2014
Independent and interactive effects of DOF affecting germination 1 (DAG1) and the Della proteins GA insensitive (GAI) and Repressor of ga1-3(RGA) in embryo development and seed germination
Boccaccini A, Santopolo S, Capauto D, Lorrai R, Minutello E, Belcram K, Palauqui JC, Costantino P, Vittorioso P. Independent and interactive effects of DOF affecting germination 1 (DAG1) and the Della proteins GA insensitive (GAI) and Repressor of ga1-3(RGA) in embryo development and seed germination. BMC Plant Biology 2014, 14: 200. PMID: 25064446, PMCID: PMC4222566, DOI: 10.1186/s12870-014-0200-z.Peer-Reviewed Original ResearchConceptsGA INSENSITIVESeed germinationEmbryo developmentDouble mutantGai-t6GERMINATION 1Mutant seedsGa1-3Germination pathwaySingle mutantsRepressorUnexpected involvementMutantsDistinct rolesGerminationGermination propertiesIndependent branchInteractive effectsGERMINATION1DAG1DormancyGenesSeedsPathwayInactivationAccumulation of heptaprenyl diphosphate sensitizes Bacillus subtilis to bacitracin: implications for the mechanism of resistance mediated by the BceAB transporter
Kingston A, Zhao H, Cook G, Helmann J. Accumulation of heptaprenyl diphosphate sensitizes Bacillus subtilis to bacitracin: implications for the mechanism of resistance mediated by the BceAB transporter. Molecular Microbiology 2014, 93: 37-49. PMID: 24806199, PMCID: PMC4077933, DOI: 10.1111/mmi.12637.Peer-Reviewed Original ResearchMUT-14 and SMUT-1 DEAD Box RNA Helicases Have Overlapping Roles in Germline RNAi and Endogenous siRNA Formation
Phillips C, Montgomery B, Breen P, Roovers E, Rim Y, Ohsumi T, Newman M, van Wolfswinkel J, Ketting R, Ruvkun G, Montgomery T. MUT-14 and SMUT-1 DEAD Box RNA Helicases Have Overlapping Roles in Germline RNAi and Endogenous siRNA Formation. Current Biology 2014, 24: 839-844. PMID: 24684932, PMCID: PMC4010136, DOI: 10.1016/j.cub.2014.02.060.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCaenorhabditis elegansCaenorhabditis elegans ProteinsDEAD-box RNA HelicasesFluoroimmunoassayGerm CellsImmunoprecipitationMolecular Sequence DataReal-Time Polymerase Chain ReactionRNA InterferenceRNA, Small InterferingSaccharomyces cerevisiaeSequence AlignmentSequence Analysis, DNAConceptsSiRNA formationMutator fociClass genesDEAD-box RNA helicasesBox RNA helicasesElegans genesEndogenous siRNAsSiRNA amplificationMutant displaysP granulesNuclear peripheryRNA helicasesMissense mutantsMutant proteinsDouble mutantMUT-16Target mRNAsRNAiGerm cellsGenesEarly stepsGermline genesSiRNAsMutantsWidespread loss
2011
Variation in Mutation Rates Caused by RB69pol Fidelity Mutants Can Be Rationalized on the Basis of Their Kinetic Behavior and Crystal Structures
Xia S, Wang M, Lee HR, Sinha A, Blaha G, Christian T, Wang J, Konigsberg W. Variation in Mutation Rates Caused by RB69pol Fidelity Mutants Can Be Rationalized on the Basis of Their Kinetic Behavior and Crystal Structures. Journal Of Molecular Biology 2011, 406: 558-570. PMID: 21216248, PMCID: PMC3059800, DOI: 10.1016/j.jmb.2010.12.033.Peer-Reviewed Original ResearchConceptsDouble mutantMutation rateAmino acid residuesRB69 DNA polymeraseSingle mutantsMutable sequencesPocket mutantsMutantsAcid residuesState kinetic parametersPrimer extensionT4 phageFidelity mutantsNucleotide residuesIncoming dNTPsDNA polymeraseReversion assayTernary complexComplementary strandCrystal structureResiduesBase selectivityPocketPolymeraseMisincorporation
2010
Interplay between Foxd3 and Mitf regulates cell fate plasticity in the zebrafish neural crest
Curran K, Lister JA, Kunkel GR, Prendergast A, Parichy DM, Raible DW. Interplay between Foxd3 and Mitf regulates cell fate plasticity in the zebrafish neural crest. Developmental Biology 2010, 344: 107-118. PMID: 20460180, PMCID: PMC2909359, DOI: 10.1016/j.ydbio.2010.04.023.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCell LineageForkhead Transcription FactorsGene Expression Regulation, DevelopmentalMelanocytesMicrophthalmia-Associated Transcription FactorMicroscopy, FluorescenceModels, BiologicalModels, GeneticMutationNeural CrestPhylogenyPigmentationZebrafishZebrafish ProteinsConceptsIridophore developmentPigment cellsCell fate plasticityZebrafish neural crestCell fate decisionsTranscription factor MITFCell lineage analysisNeural crest cellsIridescent iridophoresBlack melanophoresYellow xanthophoresMelanophore developmentFate decisionsFate plasticityTranscriptional switchDouble mutantTractable systemDanio rerioLineage analysisMelanoblast markerCrest cellsKey regulatorFoxD3Neural crestIridophoresThe Sterol Methyltransferases SMT1, SMT2, and SMT3 Influence Arabidopsis Development through Nonbrassinosteroid Products
Carland F, Fujioka S, Nelson T. The Sterol Methyltransferases SMT1, SMT2, and SMT3 Influence Arabidopsis Development through Nonbrassinosteroid Products. Plant Physiology 2010, 153: 741-756. PMID: 20421456, PMCID: PMC2879779, DOI: 10.1104/pp.109.152587.Peer-Reviewed Original ResearchConceptsDevelopmental roleSpecific sterolsDistinct developmental rolesArabidopsis developmentAuxin responseAuxin insensitivityAuxin resistanceStructural sterolsCotyledon veinFloral organsMutant resultsDouble mutantSingle mutantsFloral transformationApical dominanceProtein endocytosisNovel phenotypesWild typeDevelopmental defectsRoot growthMutantsReduced statureEnhanced defectsSmt3Cell membrane
2009
Lnk Constrains Oncogenic JAK2-Induced Myeloproliferative Disease in Mice.
Bersenev A, Wu C, Balcerek J, Tong W. Lnk Constrains Oncogenic JAK2-Induced Myeloproliferative Disease in Mice. Blood 2009, 114: 1437. DOI: 10.1182/blood.v114.22.1437.1437.Peer-Reviewed Original ResearchOncogenic JAK2TPO stimulationHematopoietic stem cell homeostasisDownstream signal transduction pathwaysStem cell homeostasisAdaptor protein LNKSignal transduction pathwaysPhysiological negative regulatorCell surface receptorsImportant signaling axisWild-type bone marrow cellsDouble mutantTransduction pathwaysJAK2 activationHSC poolLNK deficiencyNegative regulatorJanus kinaseCell homeostasisCell developmentCytokine receptorsSignaling AxisJAK2 pathwayChromosomal translocationsMPD developmentRB69 DNA Polymerase Mutants with Expanded Nascent Base-Pair-Binding Pockets Are Highly Efficient but Have Reduced Base Selectivity
Zhang H, Beckman J, Wang J, Konigsberg W. RB69 DNA Polymerase Mutants with Expanded Nascent Base-Pair-Binding Pockets Are Highly Efficient but Have Reduced Base Selectivity. Biochemistry 2009, 48: 6940-6950. PMID: 19522539, PMCID: PMC2847438, DOI: 10.1021/bi900422b.Peer-Reviewed Original ResearchConceptsBase pairsCorrect base pairReplicative DNA polymerasesRB69 polRB69 DNA Polymerase MutantsNascent base pairDouble mutantSingle mutantsTriple mutantNumber of substitutionsWild typeMutantsBacteriophage RB69DNA polymerase mutantsPolymerase mutantsDNA polymeraseBinding pocketsNegative selectionDNA polRapid incorporationCatalytic centerLow incorporation efficiencyG mutationSulfolobus solfataricus Dpo4Base discriminationThe Yb protein defines a novel organelle and regulates male germline stem cell self-renewal in Drosophila melanogaster
Szakmary A, Reedy M, Qi H, Lin H. The Yb protein defines a novel organelle and regulates male germline stem cell self-renewal in Drosophila melanogaster. Journal Of Cell Biology 2009, 185: 613-627. PMID: 19433453, PMCID: PMC2711570, DOI: 10.1083/jcb.200903034.Peer-Reviewed Original ResearchConceptsYB proteinsNiche cellsYb bodiesElectron-dense spheresDrosophila melanogasterGermline stem cell maintenanceMale germline stem cellsGermline stem cellsStem cell maintenanceStem cellsC-terminal regionSomatic stem cellsN-terminal regionD. melanogasterNovel organellePiwi expressionGermline cellsDouble mutantCell maintenanceNovel lociSomatic cellsCytoplasmic spotsMelanogasterCytoplasmic structuresGermline loss
2008
Genetic Evidence of Serum Phosphate-Independent Functions of FGF-23 on Bone
Sitara D, Kim S, Razzaque MS, Bergwitz C, Taguchi T, Schüler C, Erben RG, Lanske B. Genetic Evidence of Serum Phosphate-Independent Functions of FGF-23 on Bone. PLOS Genetics 2008, 4: e1000154. PMID: 18688277, PMCID: PMC2483943, DOI: 10.1371/journal.pgen.1000154.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone and BonesBone DensityCalcification, PhysiologicCells, CulturedFibroblast Growth Factor-23Fibroblast Growth FactorsGene ExpressionHypophosphatemiaMiceMice, Inbred C57BLMice, KnockoutMuscle, SkeletalOsteoblastsPhenotypePhosphatesSerumSkullSodium-Phosphate Cotransporter Proteins, Type IIaUrineConceptsFGF-23 geneFgf-23-/- micePhosphate homeostasisGenetic evidenceFgf-23-/-Regulation of phosphate homeostasisCrucial biological importanceFirst genetic evidenceSystemic phosphate homeostasisSkeletal mineralizationCellular functionsDouble mutantNew mouse lineMaster regulatorProtein abundanceGenomic ablationMolecular mechanismsDouble mutant miceChondrocyte differentiationTargeted disruptionSkeletal phenotypeBiological importanceGenesEnergy metabolismHomeostasis
2007
Two Gr Genes Underlie Sugar Reception in Drosophila
Dahanukar A, Lei YT, Kwon JY, Carlson JR. Two Gr Genes Underlie Sugar Reception in Drosophila. Neuron 2007, 56: 503-516. PMID: 17988633, PMCID: PMC2096712, DOI: 10.1016/j.neuron.2007.10.024.Peer-Reviewed Original ResearchConceptsSubset of sugarsReporter gene expressionSugar-sensing neuronsDouble mutantSugar receptionMolecular basisGenetic analysisGene expressionGr5aDrosophilaReceptor geneSitu hybridizationGenesNeuronsGr64aPrimary receptorTaste receptorsDistinct projectionsReceptorsTemporal dynamicsSugarsMutantsBehavioral responsesLabellumReporter
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