2025
Pancreatic β-cell apoptosis caused by apolipoprotein C3-rich low-density lipoprotein is attenuated by kansuinine A through oxidative stress inhibition
Lulji Taraqaz B, Hsu Y, Tsai P, Li Y, Chen F, Yang W, Shen M. Pancreatic β-cell apoptosis caused by apolipoprotein C3-rich low-density lipoprotein is attenuated by kansuinine A through oxidative stress inhibition. Biomedicine & Pharmacotherapy 2025, 187: 118066. PMID: 40262236, DOI: 10.1016/j.biopha.2025.118066.Peer-Reviewed Original ResearchConceptsB cell apoptosisRat pancreatic B-cellsLow-density lipoproteinPathway analysisSignaling pathwayOxidative stressApoptosisImproved cell viabilityRIN-m5FB cellsLectin-like oxidized low-density lipoprotein receptor-1Pancreatic B-cellsHigh-fat dietCell viabilityInsulin toleranceAntiapoptotic propertiesKansuinine AMitigated apoptosisMechanism of actionMolecular dockingStress inhibitionPathwayLow-density lipoprotein receptor-1Improved glucoseHigh-fatImpacts of Obesity-Induced Insulin and Leptin Dysregulation on Ovarian Function and Fertility
Rodriguez Y, Yang-Hartwich Y, Perry R. Impacts of Obesity-Induced Insulin and Leptin Dysregulation on Ovarian Function and Fertility. Physiology 2025, 40: 1996. DOI: 10.1152/physiol.2025.40.s1.1996.Peer-Reviewed Original ResearchOvarian functionOvarian tissueObesogenic contextRestore ovarian functionOvarian cell functionsResponse to hormonal changesCell viabilityImpact reproductive healthChow-fed controlsHuman granulosa-like tumorGlucose uptakeImproving fertility outcomesPotential therapeutic interventionsLeptin dysregulationCell proliferation assayHFD-fed miceLeptin treatmentGranulosa cellsLeptin resistanceOvarian environmentOvarian cellsReduced cell viabilityHormonal dysregulationLeptin signalingInsulin treatment
2024
The effect of plerixafor on autologous stem cell mobilization, cell viability, and apheresis challenges
Puzo C, Li P, Tormey C, Siddon A. The effect of plerixafor on autologous stem cell mobilization, cell viability, and apheresis challenges. Lab Medicine 2024, 56: 187-194. PMID: 39303673, DOI: 10.1093/labmed/lmae080.Peer-Reviewed Original ResearchAutologous stem cell transplantationHematopoietic stem cellsMultiple myelomaG-CSFMobilization failureDiffuse large B-cell lymphomaAutologous stem cell mobilizationLarge B-cell lymphomaGranulocyte colony-stimulating factorAutologous stem cell transplant patientsEfficacy of plerixaforStem cell mobilizationB-cell lymphomaStem cell transplantationEffects of plerixaforRetrospective chart reviewColony-stimulating factorYale-New Haven HospitalCell viabilityMultiple risk factorsHodgkin lymphomaNon-HodgkinMobilization regimenCell transplantationPlerixaforPreclinical evaluation of avutometinib and defactinib in high‐grade endometrioid endometrial cancer
Hartwich T, Mansolf M, Demirkiran C, Greenman M, Bellone S, McNamara B, Nandi S, Alexandrov L, Yang‐Hartwich Y, Coma S, Pachter J, Santin A. Preclinical evaluation of avutometinib and defactinib in high‐grade endometrioid endometrial cancer. Cancer Medicine 2024, 13: e70210. PMID: 39240189, PMCID: PMC11378359, DOI: 10.1002/cam4.70210.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntineoplastic Combined Chemotherapy ProtocolsBenzamidesCarcinoma, EndometrioidCell Line, TumorCell ProliferationEndometrial NeoplasmsExome SequencingFemaleFocal Adhesion Kinase 1HumansImidazolesMiceNeoplasm GradingOxazepinesProtein Kinase InhibitorsPyrazinesSulfonamidesXenograft Model Antitumor AssaysConceptsFocal adhesion kinaseWhole-exome sequencingEndometrial cancer cell linesVS-4718Cell linesRAS/MAPK pathwayPhosphorylated focal adhesion kinaseWestern blot assayWhole-exome sequencing resultsRAF/MEK inhibitionEAC cell linesBlot assayP-FAKGenetic landscapeCell cycleEndometrial cancerGenetic derangementsDefactinibP-MEKGrowth inhibitionRaf/MEKRas/MAPKCell viabilityP-ERKHigh-grade endometrial cancerCell-specific models reveal conformation-specific RAF inhibitor combinations that synergistically inhibit ERK signaling in pancreatic cancer cells
Sevrin T, Imoto H, Robertson S, Rauch N, Dyn'ko U, Koubova K, Wynne K, Kolch W, Rukhlenko O, Kholodenko B. Cell-specific models reveal conformation-specific RAF inhibitor combinations that synergistically inhibit ERK signaling in pancreatic cancer cells. Cell Reports 2024, 43: 114710. PMID: 39240715, PMCID: PMC11474227, DOI: 10.1016/j.celrep.2024.114710.Peer-Reviewed Original ResearchConceptsPancreatic ductal adenocarcinomaResistance to RAFResistant PDAC cellsPancreatic cancer cellsPancreatic ductal adenocarcinoma cell linesProtein expression profilesTumor-specific variationsIsogenic pairsCell-specific modelsConformational specificityERK signalingInhibitor combinationsERK pathwayKRAS mutationsTargeted therapyExpression profilesMEK inhibitorsDuctal adenocarcinomaCancer cellsKRAS mutantPhospho-ERKCell linesPDAC cellsCell viabilityDifferential sensitivityPoint-of-care human milk concentration by passive osmosis: comprehensive analysis of fresh human milk samples
Schinkel E, Nelson E, Kim J, Perrin M, Dyer R, Elango R, Bode L, Dallas D, Lueangsakulthai J, Briere C, Taylor S. Point-of-care human milk concentration by passive osmosis: comprehensive analysis of fresh human milk samples. Journal Of Perinatology 2024, 44: 1575-1583. PMID: 38760580, PMCID: PMC11518986, DOI: 10.1038/s41372-024-01988-2.Peer-Reviewed Original ResearchConceptsMilk concentrationsOsmotic concentrationHM samplesFresh HMHuman milk concentrationsMilk samplesHM componentsHuman milk samplesEnzyme activityHuman milkOptimal growthPassive osmosisFeeding parametersPost-concentrationCell viabilityTotal cell viabilityPHOsmolalityWater removalMilkConcentrationRemoval of waterMacronutrientsRemovalNS8593 inhibits sodium nitroprusside-induced chondrocyte apoptosis by mediating the STING signaling pathway
Zhu R, Hao W, Li S, Chen Y, Zhou F, Zhou R, Hu W. NS8593 inhibits sodium nitroprusside-induced chondrocyte apoptosis by mediating the STING signaling pathway. Heliyon 2024, 10: e31375. PMID: 38831839, PMCID: PMC11145487, DOI: 10.1016/j.heliyon.2024.e31375.Peer-Reviewed Original ResearchTransient receptor potential cation channel subfamily M member 7STING signaling pathwayCyclic GMP-AMP synthase (cGAS)-stimulatorSNP-induced decreaseAtrial fibrillation therapyPhosphorylation levelsSignaling pathwayApoptosis in vitroCell viabilityNS8593Atrial fibrillationDose-dependentlyImprove atrial fibrillationInterferon genesSNP-induced chondrocyte apoptosisArticular cartilage damageApoptosisChondrocyte apoptosisFeatures of osteoarthritisChondrocyte apoptosis in vitroArticular cartilage destructionCartilage destructionCartilage damageStingsTreatmentEGFR targeting PhosTACs as a dual inhibitory approach reveals differential downstream signaling
Hu Z, Chen P, Li W, Krone M, Zheng S, Saarbach J, Velasco I, Hines J, Liu Y, Crews C. EGFR targeting PhosTACs as a dual inhibitory approach reveals differential downstream signaling. Science Advances 2024, 10: eadj7251. PMID: 38536914, PMCID: PMC10971414, DOI: 10.1126/sciadv.adj7251.Peer-Reviewed Original ResearchConceptsInhibit cancer cell viabilityProteome-wide levelCancer cell viabilityDifferential signaling pathwaysPhosphoproteomic approachTyrosine dephosphorylationProtein dephosphorylationSignal transductionActivating dephosphorylationInduce apoptosisReceptor tyrosine kinase inhibitorsRTK activationSignaling pathwayInhibition of kinasesDephosphorylationEpidermal growth factor receptorGrowth factor receptorCell viabilityFactor receptorInhibitory approachesTyrosineTyrosine kinase inhibitorsInhibitory effectInhibitory potentialKinase inhibitorsNoncanonical interaction with microtubules via the N-terminal nonmotor domain is critical for the functions of a bidirectional kinesin
Singh S, Siegler N, Pandey H, Yanir N, Popov M, Goldstein-Levitin A, Sadan M, Debs G, Zarivach R, Frank G, Kass I, Sindelar C, Zalk R, Gheber L. Noncanonical interaction with microtubules via the N-terminal nonmotor domain is critical for the functions of a bidirectional kinesin. Science Advances 2024, 10: eadi1367. PMID: 38324691, PMCID: PMC10849588, DOI: 10.1126/sciadv.adi1367.Peer-Reviewed Original ResearchConceptsBidirectional motilityKinesin-5Plus-end-directed motilityKinesin-5 motorsCryo-EMC-terminal tailCryo-EM mapsPlus-end-directed kinesinCryo-electron microscopyDeletion mutantsNoncanonical interactionsIntracellular functionsCin8Cryo-electronMotor domainMotilityNonmotor domainsMutantsB-tubulinSingle-moleculeCell viabilityIn vivoIn vitroIn vitro experimentsLocal defects
2023
Optogenetic Control of Oncogenic Signaling in B-Cell Malignancies
Kume K, Lee J, Cheng Z, Robinson M, Leveille E, Cosgun K, Chan L, Feng Y, Arce D, Khanduja D, Toomre D, Müschen M. Optogenetic Control of Oncogenic Signaling in B-Cell Malignancies. Blood 2023, 142: 4138. DOI: 10.1182/blood-2023-190926.Peer-Reviewed Original ResearchB-cell malignanciesB-cell lymphomaMature B-cell lymphomasB cell deathB cellsB cell developmentGenetic deletionMantle cell lymphomaNF-kB signalingBCR signal inhibitorsB cell precursorsCell of originCell viabilityChronic active BCRB cell survivalB cell receptor signalsHodgkin's diseaseMultiple myelomaNormal B cell developmentPlasma cellsBtk tyrosine kinaseCell lymphomaBurkitt's lymphomaNF-kBSmall molecule inhibitorsAAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells
Ye L, Lam S, Yang L, Suzuki K, Zou Y, Lin Q, Zhang Y, Clark P, Peng L, Chen S. AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells. Nature Biomedical Engineering 2023, 8: 132-148. PMID: 37430157, PMCID: PMC11320892, DOI: 10.1038/s41551-023-01058-6.Peer-Reviewed Original ResearchGene delivery systemsAdeno-associated virusTransgene expressionHigh transgene expressionSleeping Beauty (SB) transposonTransgene deliverySleeping Beauty transposasePluripotent stem cellsSB transposonLentiviral vectorsGenomic integrationEngineering cellsBeauty transposonMinicircle DNATransposon DNABeauty transposaseCell viabilityPermanent integrationStem cellsElectroporation of plasmidsTransgeneChimeric antigen receptorDeliveryElectroporationEngineeringCell-free fetal DNA impairs trophoblast migration in a TLR9-dependent manner and can be reversed by hydroxychloroquine
León-Martínez D, Lynn T, Abrahams V. Cell-free fetal DNA impairs trophoblast migration in a TLR9-dependent manner and can be reversed by hydroxychloroquine. Journal Of Reproductive Immunology 2023, 157: 103945. PMID: 37062109, DOI: 10.1016/j.jri.2023.103945.Peer-Reviewed Original ResearchConceptsToll-like receptor 9Aspirin-triggered lipoxinsODN 2216Pathogenesis of preeclampsiaTLR9-dependent mannerNovel therapeutic approachesTrophoblast cell modelReceptor 9CpG oligodeoxynucleotideTLR9 inhibitorPlacental functionTherapeutic approachesCell-free fetal DNACpG motifsTherapeutic agentsHCQPreeclampsiaHydroxychloroquineFetal DNACell viabilityCffDNAMolecular underpinningsCell modelInhibitionASA
2022
Cancer Relevance of Human Genes
Qing T, Mohsen H, Cannataro VL, Marczyk M, Rozenblit M, Foldi J, Murray M, Townsend J, Kluger Y, Gerstein M, Pusztai L. Cancer Relevance of Human Genes. Journal Of The National Cancer Institute 2022, 114: 988-995. PMID: 35417011, PMCID: PMC9275765, DOI: 10.1093/jnci/djac068.Peer-Reviewed Original ResearchConceptsCore cancer genesHuman genesFunctional importanceSomatic mutation frequencySelection pressureGene/protein networksCancer genesHigher somatic mutation frequencyNegative selection pressureGene-gene interaction networksMutation frequencyProtein-truncating variantsGenomic contextCell viabilityGenes decreasesCancer Genome AtlasInteraction networksProtein networkCancer relevanceCancer cell viabilityCell survivalGenesCancer biologyGenome AtlasSearch tools
2021
Knocking down claudin receptors leads to a decrease in prostate cancer cell migration, cell growth, cell viability and clonogenic cell survival
Liu Q, Shen H, Naguib A, Weiss RM, Martin DT. Knocking down claudin receptors leads to a decrease in prostate cancer cell migration, cell growth, cell viability and clonogenic cell survival. Molecular Biomedicine 2021, 2: 31. PMID: 35006480, PMCID: PMC8607359, DOI: 10.1186/s43556-021-00053-0.Peer-Reviewed Original ResearchProstate cancer cell growthCancer cell growthProstate cancer cellsProstate cancerLNCaP cellsCommon solid organ malignancyHuman prostate cancer specimensProstate cancer cell migrationSolid organ malignanciesAdvanced prostate cancerCancer cellsHuman prostate cancer cellsNormal human prostate cellsMetastatic human prostate cancer cellsProstate cancer specimensHuman prostate cellsCell growthNew molecular targetsCell viabilityCell migrationCancer cell migrationClaudin receptorsOrgan malignanciesProstate cancer PC3Clonogenic cell survivalIntegrin β3 targeting biomaterial preferentially promotes secretion of bFGF and viability of iPSC-derived vascular smooth muscle cells
Dash BC, Duan K, Kyriakides TR, Hsia HC. Integrin β3 targeting biomaterial preferentially promotes secretion of bFGF and viability of iPSC-derived vascular smooth muscle cells. Biomaterials Science 2021, 9: 5319-5329. PMID: 34190227, DOI: 10.1039/d1bm00162k.Peer-Reviewed Original ResearchConceptsBasic fibroblast growth factorVascular smooth muscle cellsSmooth muscle cellsIntegrin β3Paracrine secretionMuscle cellsGrowth factorSecretion of bFGFMatrix metalloproteinase-2Proangiogenic growth factorsCell viabilityFibroblast growth factorHuman-induced pluripotent stemMetalloproteinase-2Ligand-integrin interactionExtracellular matrix microenvironmentSecretionPossible rolePositive feedback loopPaucity of researchMatrix microenvironmentIntegrin interactionPluripotent stemFibrillar collagenΒ3Protein Phosphatase 2A as a Therapeutic Target in Small Cell Lung Cancer
Mirzapoiazova T, Xiao G, Mambetsariev B, Nasser MW, Miaou E, Singhal SS, Srivastava S, Mambetsariev I, Nelson MS, Nam A, Behal A, Arvanitis LD, Atri P, Muschen M, Tissot FLH, Miser J, Kovach JS, Sattler M, Batra SK, Kulkarni P, Salgia R. Protein Phosphatase 2A as a Therapeutic Target in Small Cell Lung Cancer. Molecular Cancer Therapeutics 2021, 20: 1820-1835. PMID: 34253596, PMCID: PMC8722383, DOI: 10.1158/1535-7163.mct-21-0013.Peer-Reviewed Original ResearchConceptsProtein phosphatase 2APhosphatase 2ASerine/threonine phosphataseDNA damage responseRegulation of apoptosisSmall molecule inhibitorsGlycolytic ATP productionThreonine phosphataseTwo-dimensional cultureLB100ATP productionMolecule inhibitorsPP2AThree-dimensional spheroid modelEndothelial cell monolayersGlucose uptakeCell viabilitySCLC cellsTherapeutic targetApoptosisCell monolayersMass spectrometrySpheroid modelTumor spheroidsCellsPepsin Promotes Activation of Epidermal Growth Factor Receptor and Downstream Oncogenic Pathways, at Slightly Acidic and Neutral pH, in Exposed Hypopharyngeal Cells
Doukas PG, Vageli DP, Sasaki CT, Judson BL. Pepsin Promotes Activation of Epidermal Growth Factor Receptor and Downstream Oncogenic Pathways, at Slightly Acidic and Neutral pH, in Exposed Hypopharyngeal Cells. International Journal Of Molecular Sciences 2021, 22: 4275. PMID: 33924087, PMCID: PMC8074291, DOI: 10.3390/ijms22084275.Peer-Reviewed Original ResearchConceptsRelated-signaling pathwaysOncogenic pathwaysLaryngopharyngeal reflux diseaseEpidermal growth factor receptorSpecific oncogenic pathwaysDownstream oncogenic pathwaysGrowth factor receptorHypopharyngeal cellsReflux diseaseRisk factorsTherapeutic approachesIntermittent exposureTumorigenic effectsPronounced activationOncogenic factorSignificant upregulationFactor receptorSTAT3 activationAcidic pepsinEGFR activationCell viabilityPromotes ActivationActivationPrimary cellsCellsDown-regulation of GP130 signaling sensitizes bladder cancer to cisplatin by impairing Ku70 DNA repair signaling and promoting apoptosis
He S, Li G, Schätzlein AG, Humphrey PA, Weiss RM, Uchegbu IF, Martin DT. Down-regulation of GP130 signaling sensitizes bladder cancer to cisplatin by impairing Ku70 DNA repair signaling and promoting apoptosis. Cellular Signalling 2021, 81: 109931. PMID: 33529758, DOI: 10.1016/j.cellsig.2021.109931.Peer-Reviewed Original ResearchConceptsBladder cancer cellsCancer cellsHuman bladder cancer specimensCisplatin-based chemotherapyBladder cancer treatmentBladder cancer specimensChemoresistant bladder cancer cellsBreast cancer cellsCell viabilityBladder cancerCancer specimensSmall molecule inhibitorsLevels of Ku70Cancer treatmentKu70 expressionWhole-genome doubling confers unique genetic vulnerabilities on tumour cells
Quinton R, DiDomizio A, Vittoria M, Kotýnková K, Ticas C, Patel S, Koga Y, Vakhshoorzadeh J, Hermance N, Kuroda T, Parulekar N, Taylor A, Manning A, Campbell J, Ganem N. Whole-genome doubling confers unique genetic vulnerabilities on tumour cells. Nature 2021, 590: 492-497. PMID: 33505027, PMCID: PMC7889737, DOI: 10.1038/s41586-020-03133-3.Peer-Reviewed Original ResearchConceptsWhole-genome doublingUnstable tetraploid cellsAccurate chromosome segregationDNA replication factorsSpindle assembly checkpointPrimary human cancer samplesHuman cancer samplesEssentiality dataChromosome segregationUnique genetic vulnerabilitiesTetraploid stateKinesin proteinsProteasome functionMitotic errorsGenetic traitsTetraploid cellsHuman cancersCancer cell linesCancer cellsCell linesKIF18ACell viabilityCancer samplesHuman tissuesCells
2020
Nerve growth factor promotes ASIC1a expression via the NF-κB pathway and enhances acid-induced chondrocyte apoptosis
Wei X, Sun C, Zhou R, Ma G, Yang Y, Lu C, Hu W. Nerve growth factor promotes ASIC1a expression via the NF-κB pathway and enhances acid-induced chondrocyte apoptosis. International Immunopharmacology 2020, 82: 106340. PMID: 32146316, DOI: 10.1016/j.intimp.2020.106340.Peer-Reviewed Original ResearchNerve growth factorNF-κB p65 activationMediators of inflammationNF-κB p65Cleaved-PARP expressionNF-κB inhibitorArticular chondrocytesApoptotic protein expressionASIC1a expressionTime-dependent fashionNeurotrophic factorP65 activationNF-κBNervous systemAcid-induced apoptosisLDH releaseChondrocyte apoptosisRat chondrocytesGrowth factorProtein expressionCell viabilityApoptosisChondrocytesExpressionInflammation
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