2025
Identification of F-box proteins in ABA- and GA-regulated seed germination: interaction of GASA1 signalling peptide and ABA-induced ubiquitination
Kasera M, Ceciliato P, Lopez B, Hauser F, Gendron J, Schroeder J. Identification of F-box proteins in ABA- and GA-regulated seed germination: interaction of GASA1 signalling peptide and ABA-induced ubiquitination. Philosophical Transactions Of The Royal Society B Biological Sciences 2025, 380: 20240233. PMID: 40439299, PMCID: PMC12121378, DOI: 10.1098/rstb.2024.0233.Peer-Reviewed Original ResearchConceptsSeed germinationSignal peptideGenetic screeningF-box proteinsForward genetic screensHomology-based gene silencingInhibition of seed germinationABA-insensitiveHomologous genesFunctional genomicsGene familyTriple mutantF-boxABA inhibitionProteasome pathwayAmiRNA linesGene silencingInteraction partnersGerminating seedsArtificial microRNAGenesABAGerminationMutantsUbiquitin
2022
The landscape of pioneer factor activity reveals the mechanisms of chromatin reprogramming and genome activation
Miao L, Tang Y, Bonneau AR, Chan SH, Kojima ML, Pownall ME, Vejnar CE, Gao F, Krishnaswamy S, Hendry CE, Giraldez AJ. The landscape of pioneer factor activity reveals the mechanisms of chromatin reprogramming and genome activation. Molecular Cell 2022, 82: 986-1002.e9. PMID: 35182480, PMCID: PMC9327391, DOI: 10.1016/j.molcel.2022.01.024.Peer-Reviewed Original ResearchConceptsGenome activationChromatin openingTranscription factorsPioneer factor activityDifferent transcription factorsChromatin reprogrammingPioneer factorsNucleosome positionsActive enhancersIndividual genesCore histonesTriple mutantGene activationTF inputsDevelopmental transitionsSequence contextCell typesFactor activityHistonesPioneering activityEnhancerActivationSequence of eventsPou5f3Chromatin
2019
Reengineering a tRNA Methyltransferase To Covalently Capture New RNA Substrates
Smith TS, Zoltek MA, Simon MD. Reengineering a tRNA Methyltransferase To Covalently Capture New RNA Substrates. Journal Of The American Chemical Society 2019, 141: 17460-17465. PMID: 31626536, DOI: 10.1021/jacs.9b08529.Peer-Reviewed Original ResearchConceptsSubstrate specificityRNA substratesSubstrate RNARNA substrate specificityWild-type proteinCovalent RNA modificationsHigh-throughput sequencing assaysDouble mutantRNA modificationsTriple mutantSubstrate mutantsTRNA methyltransferaseProtein engineeringRNA transcriptsMutantsRational engineeringRNASubstrate screeningTRMASequencing assaysMethyltransferaseEnzymeProteinPowerful toolSpecificity
2013
Six Innexins Contribute to Electrical Coupling of C. elegans Body-Wall Muscle
Liu P, Chen B, Altun Z, Gross M, Shan A, Schuman B, Hall D, Wang Z. Six Innexins Contribute to Electrical Coupling of C. elegans Body-Wall Muscle. PLOS ONE 2013, 8: e76877. PMID: 24130800, PMCID: PMC3793928, DOI: 10.1371/journal.pone.0076877.Peer-Reviewed Original ResearchConceptsUNC-9Body wall musclesC. elegans body wall muscleBody wall muscle cellsGap junctionsEpitope-tagged proteinsInnexinsTriple mutantPunctate localizationFunctions cellMutantsIntercellular junctionsDistinct populationsMuscle expressionGFPMuscle cellsJunctional currentsElectrical couplingFunctional propertiesCellsPromoterProteinSolid foundationExpressionPopulation
2009
RB69 DNA Polymerase Mutants with Expanded Nascent Base-Pair-Binding Pockets Are Highly Efficient but Have Reduced Base Selectivity
Zhang H, Beckman J, Wang J, Konigsberg W. RB69 DNA Polymerase Mutants with Expanded Nascent Base-Pair-Binding Pockets Are Highly Efficient but Have Reduced Base Selectivity. Biochemistry 2009, 48: 6940-6950. PMID: 19522539, PMCID: PMC2847438, DOI: 10.1021/bi900422b.Peer-Reviewed Original ResearchConceptsBase pairsCorrect base pairReplicative DNA polymerasesRB69 polRB69 DNA Polymerase MutantsNascent base pairDouble mutantSingle mutantsTriple mutantNumber of substitutionsWild typeMutantsBacteriophage RB69DNA polymerase mutantsPolymerase mutantsDNA polymeraseBinding pocketsNegative selectionDNA polRapid incorporationCatalytic centerLow incorporation efficiencyG mutationSulfolobus solfataricus Dpo4Base discrimination
2008
Structural basis for base discrimination by RB69 DNA polymerase
Wang M, Klimenko D, Steitz T, Wang J. Structural basis for base discrimination by RB69 DNA polymerase. The FASEB Journal 2008, 22: 593.2-593.2. DOI: 10.1096/fasebj.22.1_supplement.593.2.Peer-Reviewed Original ResearchTriple mutantApo formStructural basisBase pairsDNA polymeraseReplicative DNA polymerasesWild-type enzymeTernary complexTemplating baseHelix PBase selectivityNascent base pairRB69 DNA polymeraseBase discriminationWild-type PolType enzymeMismatched base pairsMutantsPol mutantsRB69 polPolymeraseComplexesS565Y416Pol
1997
Growth-Promoting Interaction of IGF-II with the Insulin Receptor during Mouse Embryonic Development
Louvi A, Accili D, Efstratiadis A. Growth-Promoting Interaction of IGF-II with the Insulin Receptor during Mouse Embryonic Development. Developmental Biology 1997, 189: 33-48. PMID: 9281335, DOI: 10.1006/dbio.1997.8666.Peer-Reviewed Original ResearchConceptsInsulin receptorGrowth-promoting interactionsMouse embryonic developmentMouse embryonic growthLigand/receptor interactionsGrowth-promoting functionEmbryonic day 13.5IGF-IINull mutantsTargeted MutagenesisGrowth of embryosMouse embryogenesisDouble mutantTriple mutantEmbryonic developmentGenetic evidenceIGF1R resultsOrgan hypoplasiaGenetic analysisMutantsEmbryonic growthType 1 IGF receptorDay 13.5Receptor interactionEmbryos
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