2024
Quantification and Site-Specific Analysis of Co-occupied N- and O‑Glycopeptides
Chongsaritsinsuk J, Rangel-Angarita V, Lucas T, Mahoney K, Enny O, Katemauswa M, Malaker S. Quantification and Site-Specific Analysis of Co-occupied N- and O‑Glycopeptides. Journal Of Proteome Research 2024, 23: 5449-5461. PMID: 39498894, PMCID: PMC12057997, DOI: 10.1021/acs.jproteome.4c00574.Peer-Reviewed Original ResearchElectron-based methodsO-glycopeptidesElectron-based fragmentation methodsFragmentation methodCo-occupancyO-glycosylated peptidesAnalysis of glycopeptidesPost-translational modificationsN-glycoproteomic analysisCore 1 structureComplex samplesO-glycansPurified proteinProtein glycosylationO-glycositesN-glycoproteomeNonmucin glycoproteinsTryptic peptidesDissociation methodO-linkedAnalysis of mucinsSite-specific analysisGlycoproteinGlycosylationProteinTRF2–RAP1 represses RAD51-dependent homology-directed telomere repair by promoting BLM-mediated D-loop unwinding and inhibiting BLM–DNA2-dependent 5′-end resection
Liang F, Rai R, Sodeinde T, Chang S. TRF2–RAP1 represses RAD51-dependent homology-directed telomere repair by promoting BLM-mediated D-loop unwinding and inhibiting BLM–DNA2-dependent 5′-end resection. Nucleic Acids Research 2024, 52: 9695-9709. PMID: 39082275, PMCID: PMC11381343, DOI: 10.1093/nar/gkae642.Peer-Reviewed Original ResearchHomology-directed repairTelomeric D-loopsD-loopChromosome fusionsD-loop formationSingle-stranded telomeric overhangsHomology searchTelomere clusteringTRFH domainPurified proteinBasic domainBlm mutantsProtect telomeresGenomic instabilityTelomeric overhangEnd resectionTRF2Molecular mechanismsTelomereTelomere lossMolecular pathwaysTelomere repairGenomeMutantsRap1
2016
Determining antigen specificity of a monoclonal antibody using genome-scale CRISPR-Cas9 knockout library
Zotova A, Zotov I, Filatov A, Mazurov D. Determining antigen specificity of a monoclonal antibody using genome-scale CRISPR-Cas9 knockout library. Journal Of Immunological Methods 2016, 439: 8-14. PMID: 27664857, DOI: 10.1016/j.jim.2016.09.006.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntibody SpecificityCell LineCell SeparationCRISPR-Cas SystemsEpitopesFlow CytometryGene EditingGene Knockout TechniquesGene LibraryHigh-Throughput Nucleotide SequencingHuman T-lymphotropic virus 1HumansHybridomasKangai-1 ProteinLeukocyte Common AntigensTransfectionConceptsShort-interfering RNAGuide-RNACRISPR-Cas9 knockout libraryGene-based methodsRaji B cell lineCRISPR-Cas9 knockoutDeep sequencing analysisGene-based techniquesGeCKO libraryProtein-based methodsKnockout libraryLibrary preparationValidation of antibodiesB cell linesPurified proteinPooled librariesCDNA expressionImmunoprecipitation assaysWhole cellsMonoclonal antibodiesCell sortingViral biofilmsCurrent proteinsAntibody validationAntigen-Specific
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