2023
Functional Analysis of a Novel Complement C5a Receptor 1-Blocking Monoclonal Antibody
Cyranka L, Mariegaard I, Skjødt M, Bayarri-Olmos R, Mollnes T, Garred P, Rosbjerg A. Functional Analysis of a Novel Complement C5a Receptor 1-Blocking Monoclonal Antibody. Journal Of Innate Immunity 2023, 15: 836-849. PMID: 37952515, PMCID: PMC10691831, DOI: 10.1159/000535084.Peer-Reviewed Original ResearchConceptsC5a receptor 1C5aR1 inhibitionPolymorphonuclear leukocytesComplement C5a receptor 1Receptor 1Monoclonal antibodiesWhole bloodActivation markers CD11bIsolated polymorphonuclear leukocytesPotential therapeutic candidateInflammatory disease treatmentWhole blood modelCalcium flux assaysCell-binding studiesMouse monoclonal antibodyC5a-C5aR1Marker CD11bTherapeutic candidateHuman whole bloodC5aCalcium fluxComplement systemC5aR1Hybridoma clonesDisease treatment
2021
Relationship of Multiplex Molecular Pneumonia Panel Results With Hospital Outcomes and Clinical Variables
Rand K, Beal S, Cherabuddi K, Houck H, Lessard K, Tremblay E, Couturier B, Lingenfelter B, Rindlisbacher C, Jones J. Relationship of Multiplex Molecular Pneumonia Panel Results With Hospital Outcomes and Clinical Variables. Open Forum Infectious Diseases 2021, 8: ofab368. PMID: 34458392, PMCID: PMC8391091, DOI: 10.1093/ofid/ofab368.Peer-Reviewed Original ResearchPneumonia PanelBronchoalveolar lavageInflammatory responseGroup 1Percent polymorphonuclear leukocytesBioFire FilmArray Pneumonia PanelAntibiotic treatment decisionsConsecutive bronchoalveolar lavagesWhite blood cellsEndotracheal specimensHospital outcomesIll patientsAntimicrobial resistance markersClinical variablesAntibiotic stewardshipMicrobiologic resultsLaboratory turnaround timeTreatment decisionsGroup 2Group 3Drug AdministrationPolymorphonuclear leukocytesPatientsRoutine cultureBlood cells
2014
Reduced bioenergetics and toll-like receptor 1 function in human polymorphonuclear leukocytes in aging
Qian F, Guo X, Wang X, Yuan X, Chen S, Malawista SE, Bockenstedt LK, Allore HG, Montgomery RR. Reduced bioenergetics and toll-like receptor 1 function in human polymorphonuclear leukocytes in aging. Aging 2014, 6: 131-139. PMID: 24595889, PMCID: PMC3969281, DOI: 10.18632/aging.100642.Peer-Reviewed Original ResearchConceptsToll-like receptor 1Polymorphonuclear leukocytesOlder adultsChemokine IL-8Loss of CD62LAge-dependent deficienciesPotential therapeutic opportunitiesHuman polymorphonuclear leukocytesTLR1 expressionIL-8PMN functionPMN activationMultiple pathwaysImmune functionYoung donorsP38 MAP kinaseReceptor 1Bacterial infectionsTherapeutic opportunitiesProgressive declineGlucose uptakeReduced expressionAdultsStimulationLeukocytes
2010
A Paradoxical Role for Neutrophils in the Pathogenesis of West Nile Virus
Bai F, Kong KF, Dai J, Qian F, Zhang L, Brown CR, Fikrig E, Montgometry R. A Paradoxical Role for Neutrophils in the Pathogenesis of West Nile Virus. The Journal Of Infectious Diseases 2010, 202: 1804-1812. PMID: 21050124, PMCID: PMC3053000, DOI: 10.1086/657416.Peer-Reviewed Original ResearchConceptsWest Nile virusPolymorphonuclear leukocytesWNV infectionNile virusHigh viremiaViral clearanceEarly deathEarly infectionControl groupProtective roleBiphasic responseInnate immunityViral pathogenesisInfectionMiceViremiaPathogenesisParadoxical roleEfficient replicationVirusCXCL1CXCL2ChemokinesCXCR2NeutrophilsP‐Rex1 regulates lung microvascular permeability
Naikawadi R, Cheng N, Wu D, Ye R. P‐Rex1 regulates lung microvascular permeability. The FASEB Journal 2010, 24: lb554-lb554. DOI: 10.1096/fasebj.24.1_supplement.lb554.Peer-Reviewed Original ResearchAcute lung injuryLung microvascular permeabilityPolymorphonuclear leukocytesLung injuryEndothelial cellsP-Rex1 expressionMicrovascular permeabilityLung microvascular endothelial cellsGram-negative bacterial infectionsTNF-alpha contributesWild-type miceMicrovascular endothelial cellsEndothelial barrier permeabilityP-Rex1Endothelial cell signalingBacterial clearanceTNF-alphaReactive oxygen species generationBarrier permeabilityType miceMajor cytokineKnockout miceBacterial infectionsSepticemic infectionUncontrolled activation
2009
Inhibition of Neutrophil Function by Two Tick Salivary Proteins
Guo X, Booth CJ, Paley MA, Wang X, DePonte K, Fikrig E, Narasimhan S, Montgomery RR. Inhibition of Neutrophil Function by Two Tick Salivary Proteins. Infection And Immunity 2009, 77: 2320-2329. PMID: 19332533, PMCID: PMC2687334, DOI: 10.1128/iai.01507-08.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesPMN functionNumber of PMNPMN integrinsPMN adherenceNeutrophil functionSpirochete burdenTick salivary proteinsTick salivaLyme diseaseTick attachmentSalivary glandsBorrelia burgdorferiTick feedingCausative agentReduced levelsInhibitory proteinSalivaBlood mealAntihemostatic activityInfectionInhibitionSalivary proteinsHematophagous arthropodsTick Ixodes scapularis
2007
Blocking p21-activated Kinase Reduces Lipopolysaccharide-induced Acute Lung Injury by Preventing Polymorphonuclear Leukocyte Infiltration
Reutershan J, Stockton R, Zarbock A, Sullivan GW, Chang D, Scott D, Schwartz MA, Ley K. Blocking p21-activated Kinase Reduces Lipopolysaccharide-induced Acute Lung Injury by Preventing Polymorphonuclear Leukocyte Infiltration. American Journal Of Respiratory And Critical Care Medicine 2007, 175: 1027-1035. PMID: 17322107, PMCID: PMC1899271, DOI: 10.1164/rccm.200612-1822oc.Peer-Reviewed Original ResearchConceptsAcute lung injuryLung injuryPMN migrationPolymorphonuclear leukocytesAlveolar spaceLipopolysaccharide-induced lung injuryMurine lung injuryPolymorphonuclear leukocyte infiltrationLeukocyte-endothelial interactionsAlveolo-capillary membraneOxidative burstRole of PAKsLeukocyte infiltrationChemokine receptorsLung interstitiumMurine modelExcessive recruitmentPMN chemoattractantInjuryCytoskeletal actin polymerizationAdhesion moleculesCritical mediatorCell migrationAttractive targetPAK phosphorylation
2006
Recruitment of Macrophages and Polymorphonuclear Leukocytes in Lyme Carditis
Montgomery RR, Booth CJ, Wang X, Blaho VA, Malawista SE, Brown CR. Recruitment of Macrophages and Polymorphonuclear Leukocytes in Lyme Carditis. Infection And Immunity 2006, 75: 613-620. PMID: 17101663, PMCID: PMC1828503, DOI: 10.1128/iai.00685-06.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBorrelia burgdorferiColony Count, MicrobialCytokinesDisease Models, AnimalDisease SusceptibilityDNA, BacterialHeartHistocytochemistryLyme DiseaseMacrophagesMiceMice, Inbred C3HMice, Inbred C57BLMice, KnockoutMyocarditisMyocardiumNeutrophilsPolymerase Chain ReactionReceptors, CCR2Receptors, ChemokineUrinary BladderConceptsLyme carditisPolymorphonuclear leukocytesC3H micePresence of PMNsB. burgdorferi burdenNeutrophil chemokine receptorOrgan-specific pathogenesisChemokine receptor CCR2B. burgdorferiRecruitment of macrophagesWild-type miceB. burgdorferi infectionAbsence of macrophagesFunction of macrophagesPeak diseaseInfected heartsLyme arthritisSevere arthritisHeart lesionsReceptor CCR2Severe inflammationHistopathologic examinationChemokine receptorsBurgdorferi infectionCarditis
2003
Calprotectin, an Abundant Cytosolic Protein from Human Polymorphonuclear Leukocytes, Inhibits the Growth of Borrelia burgdorferi
Lusitani D, Malawista SE, Montgomery RR. Calprotectin, an Abundant Cytosolic Protein from Human Polymorphonuclear Leukocytes, Inhibits the Growth of Borrelia burgdorferi. Infection And Immunity 2003, 71: 4711-4716. PMID: 12874352, PMCID: PMC166021, DOI: 10.1128/iai.71.8.4711-4716.2003.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesB. burgdorferiGranule-poor cytoplastsBorrelia burgdorferiBlood polymorphonuclear leukocytesHuman blood polymorphonuclear leukocytesNumerous polymorphonuclear leukocytesPotent bacteriostatic agentHuman polymorphonuclear leukocytesU-CytAcute inflammationCalprotectinModulatory roleLyme spirocheteLyme diseaseSpecific antibodiesProtein calprotectinBurgdorferiLeukocytesAbundant cytosolic proteinOxidative mechanismsBacteriostatic agentRegrowth assaysQuantitative microscopicInflammationMollaret's meningitis: Cytopathologic analysis of fourteen cases
Chan TY, Parwani AV, Levi AW, Ali SZ. Mollaret's meningitis: Cytopathologic analysis of fourteen cases. Diagnostic Cytopathology 2003, 28: 227-231. PMID: 12722116, DOI: 10.1002/dc.10261.Peer-Reviewed Original ResearchConceptsMollaret's meningitisRecurrent episodesPolymerase chain reactionPolymorphonuclear leukocytesMost common clinical presentationCerebrospinal fluid examinationCommon clinical presentationAppropriate clinical settingSmall mature lymphocytesHerpes simplex virus type 2 DNAHemosiderin-laden macrophagesCentral nervous systemVirus type 2 DNASole diagnostic modalityAseptic meningitisCSF examinationFluid examinationClinical presentationLymphoproliferative disordersMild feverClinical dataCytopathologic analysisDifferential diagnosisPlasma cellsDiagnostic modalities
2002
Superoxide Anion Production during Anaplasma phagocytophila Infection
Wang T, Malawista SE, Pal U, Grey M, Meek J, Akkoyunlu M, Thomas V, Fikrig E. Superoxide Anion Production during Anaplasma phagocytophila Infection. The Journal Of Infectious Diseases 2002, 186: 274-280. PMID: 12134266, DOI: 10.1086/341451.Peer-Reviewed Original ResearchConceptsChronic granulomatous diseaseRespiratory burstA. phagocytophilaRespiratory burst inhibitionNitroblue tetrazoliumPopulation of neutrophilsSuperoxide anion productionInfected miceGranulomatous diseaseMouse modelUse of assaysPolymorphonuclear leukocytesUninfected animalsAnaplasma phagocytophilaAnion productionNeutrophilsInfectionHL-60 cellsIndividual cell basisAssaysCell basisCellsPatientsLeukocytesDiseaseHuman phagocytic cells in the early innate immune response to Borrelia burgdorferi
Montgomery RR, Lusitani D, de Boisfleury Chevance A, Malawista SE. Human phagocytic cells in the early innate immune response to Borrelia burgdorferi. The Journal Of Infectious Diseases 2002, 185: 1773-1779. PMID: 12085324, DOI: 10.1086/340826.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesImmune responseEarly innate immune responseKilling of spirochetesSpecific antibodiesBorrelia burgdorferiSecondary immune responseInnate immune responseInnate immune systemHuman phagocytic cellsSpirochete clearanceMononuclear cellsImmune systemLyme diseasePhagocytic cellsNatural infectionMature macrophagesSpirochetesIntracellular colocalizationAntibodiesBurgdorferiFirst cellsLimited uptakeCellsMonocytesBorrelia burgdorferi Are Susceptible to Killing by a Variety of Human Polymorphonuclear Leukocyte Components
Lusitani D, Malawista SE, Montgomery RR. Borrelia burgdorferi Are Susceptible to Killing by a Variety of Human Polymorphonuclear Leukocyte Components. The Journal Of Infectious Diseases 2002, 185: 797-804. PMID: 11920297, DOI: 10.1086/339341.Peer-Reviewed Original ResearchConceptsIntact polymorphonuclear leukocytesPolymorphonuclear leukocytesB. burgdorferiKilling of BorreliaHuman neutrophil peptides 1Neutrophil peptide-1Bactericidal/permeability-increasing proteinIntact human polymorphonuclear leukocytesHuman polymorphonuclear leukocytesPermeability-increasing proteinProteinase 3Peptide-1Leukocyte componentsNitric oxideLL-37Specific antibodiesBorrelia burgdorferiBurgdorferiGranule contentsEfficient killingSpirochetesCritical roleKillingLeukocytes
2000
PECAM-1 (CD31) Expression Modulates Bleeding Time in Vivo
Mahooti S, Graesser D, Patil S, Newman P, Duncan G, Mak T, Madri J. PECAM-1 (CD31) Expression Modulates Bleeding Time in Vivo. American Journal Of Pathology 2000, 157: 75-81. PMID: 10880378, PMCID: PMC1850208, DOI: 10.1016/s0002-9440(10)64519-1.Peer-Reviewed Original ResearchConceptsPECAM-1-deficient micePECAM-1Endothelial cellsNormal bleeding timeWild-type miceHematopoietic precursorsPolymorphonuclear leukocyte (PMN) transmigrationPECAM-1 expressionBleeding timePolymorphonuclear leukocytesAdjacent endothelial cellsMiceEndothelial cell migrationLeukocyte transmigrationBlood elementsExpression modulatesThrombosisPECAM-1 functionsHumanized In Vivo Model for Streptococcal Impetigo
Scaramuzzino D, McNiff J, Bessen D. Humanized In Vivo Model for Streptococcal Impetigo. Infection And Immunity 2000, 68: 2880-2887. PMID: 10768985, PMCID: PMC97500, DOI: 10.1128/iai.68.5.2880-2887.2000.Peer-Reviewed Original ResearchConceptsStreptococcal impetigoVivo modelPattern AGross pathology scoresD strainsHuman neonatal foreskinEmm pattern ASevere infectionsEpidemiological findingsPathology scoresPurulent exudateSCID micePolymorphonuclear leukocytesPositive cocciNeonatal foreskinImpetigoC strainDays postinoculationMouse originVirulent strainGranular keratinocytesIntact epidermisStratum corneumCocciEpidermis
1997
Immunization against the agent of human granulocytic ehrlichiosis in a murine model.
Sun W, IJdo JW, Telford SR, Hodzic E, Zhang Y, Barthold SW, Fikrig E. Immunization against the agent of human granulocytic ehrlichiosis in a murine model. Journal Of Clinical Investigation 1997, 100: 3014-3018. PMID: 9399947, PMCID: PMC508513, DOI: 10.1172/jci119855.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisGranulocytic ehrlichiosisC3H/HeN miceAgent of HGEMimic human diseaseTick-borne infectionsTransient neutropeniaHeN miceMurine modelPolymorphonuclear leukocytesTick-borne pathogensAoHGEMiceImmunityEhrlichiosisSyringe inoculationHuman diseasesAgentsNeutropeniaImmunizationInfectionLeukocytesDiseaseAntibodies
1992
Evidence for reactive nitrogen intermediates in killing of staphylococci by human neutrophil cytoplasts. A new microbicidal pathway for polymorphonuclear leukocytes.
Malawista SE, Montgomery RR, van Blaricom G. Evidence for reactive nitrogen intermediates in killing of staphylococci by human neutrophil cytoplasts. A new microbicidal pathway for polymorphonuclear leukocytes. Journal Of Clinical Investigation 1992, 90: 631-636. PMID: 1379614, PMCID: PMC443143, DOI: 10.1172/jci115903.Peer-Reviewed Original ResearchConceptsReactive nitrogen intermediatesL-arginineNitric oxide synthase inhibitor NGranule-poor cytoplastsKilling of staphylococciNitrogen intermediatesSynthase inhibitor NEffect of NMMAChronic granulomatous diseaseHuman blood neutrophilsHuman neutrophil cytoplastsBlood neutrophilsGranulomatous diseasePolymorphonuclear leukocytesD-arginineIntact PMNsNeutrophil cytoplastsMicrobicidal pathwaysNMMASusceptible targetsMin of incubationModest decreaseInhibitor NStaphylococciOxidase activity
1991
Human Polymorphonuclear Leukocytes Generate and Degrade Endothelin-1 by Two Distinct Neutral Proteases
Sessa W, Kaw S, Zembowicz A, Änggård E, Hecker M, Vane J. Human Polymorphonuclear Leukocytes Generate and Degrade Endothelin-1 by Two Distinct Neutral Proteases. Journal Of Cardiovascular Pharmacology 1991, 17: s34-38. PMID: 1725374, DOI: 10.1097/00005344-199100177-00010.Peer-Reviewed Original ResearchConceptsET-1Big endothelinEndothelin-1Important pathophysiologic implicationsHuman polymorphonuclear leukocytesFMLP-activated PMNHuman big endothelinContractile factorsPMN infiltrationLeukocyte cathepsin GContractile activityPathophysiologic implicationsActivated PMNsPolymorphonuclear leukocytesNeutral proteaseDisease statesCell-free supernatantCathepsin GPMNPhosphoramidonMetabolismThe biosynthesis of endothelin-1 by human polymorphonuclear leukocytes
Sessa W, Kaw S, Hecker M, Vane J. The biosynthesis of endothelin-1 by human polymorphonuclear leukocytes. Biochemical And Biophysical Research Communications 1991, 174: 613-618. PMID: 1993057, DOI: 10.1016/0006-291x(91)91461-k.Peer-Reviewed Original Research
1989
Effects of Intravenous Fat Infusion on Neonatal Neutrophil and Platelet Function
Herson V, Block C, Eisenfeld L, Maderazo E, Krause P. Effects of Intravenous Fat Infusion on Neonatal Neutrophil and Platelet Function. Journal Of Parenteral And Enteral Nutrition 1989, 13: 620-622. PMID: 2515309, DOI: 10.1177/0148607189013006620.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesIntralipid infusionNeonatal intensive care unitAdult polymorphonuclear leukocytesIntensive care unitIntravenous fat infusionPresence of infectionAdverse hematologic effectsNeonatal polymorphonuclear leukocytesParenteral nutritionIll neonatesNeonatal neutrophilsPMN countCare unitPlatelet countPlatelet activityHematologic effectsIntravenous fatPlatelet functionPlatelet numberUnchanged preFat infusionPlatelet aggregationInfusionIntralipid
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