2022
Super‐killer CTLs are generated by single gene deletion of Bach2
Barton P, Davenport A, Hukelmann J, Cantrell D, Stinchcombe J, Richard A, Griffiths G. Super‐killer CTLs are generated by single gene deletion of Bach2. European Journal Of Immunology 2022, 52: 1776-1788. PMID: 36086884, PMCID: PMC9828676, DOI: 10.1002/eji.202249797.Peer-Reviewed Original ResearchConceptsCD8<sup>+</sup> T cellsT cellsEffector CTLSplenic CD8<sup>+</sup> T cellsT cell-mediated immune regulationMurine CD8<sup>+</sup> T cellsCentral memory T cellsWild-type CTLMemory T cellsNaive T cellsGene deletionAbsence of Bach2In vitro activityCytolytic capacityBach2 deficiencyNaive cellsEnhanced cytotoxicityImmune regulationCytolytic granulesCytotoxic advantageCTLBach2Wild-typeDifferentiation stateGranule contents
2020
Programmed ‘disarming’ of the neutrophil proteome reduces the magnitude of inflammation
Adrover JM, Aroca-Crevillén A, Crainiciuc G, Ostos F, Rojas-Vega Y, Rubio-Ponce A, Cilloniz C, Bonzón-Kulichenko E, Calvo E, Rico D, Moro MA, Weber C, Lizasoaín I, Torres A, Ruiz-Cabello J, Vázquez J, Hidalgo A. Programmed ‘disarming’ of the neutrophil proteome reduces the magnitude of inflammation. Nature Immunology 2020, 21: 135-144. PMID: 31932813, PMCID: PMC7223223, DOI: 10.1038/s41590-019-0571-2.Peer-Reviewed Original ResearchConceptsMagnitude of inflammationNeutrophil extracellular trapsNeutrophil proteomeGranule contentsInflammatory injuryRespiratory distressPneumonia patientsExtracellular trapsCell-intrinsic programsProgressive lossHuman neutrophilsNET formationProtein storesNeutrophilsInflammationLungAntimicrobial functionMouse mutantsCircadian cyclePatientsInjuryTime of dayArmamentariumIncidenceSeverity
2013
A Network of Interactions Enables CCM3 and STK24 to Coordinate UNC13D-Driven Vesicle Exocytosis in Neutrophils
Zhang Y, Tang W, Zhang H, Niu X, Xu Y, Zhang J, Gao K, Pan W, Boggon TJ, Toomre D, Min W, Wu D. A Network of Interactions Enables CCM3 and STK24 to Coordinate UNC13D-Driven Vesicle Exocytosis in Neutrophils. Developmental Cell 2013, 27: 215-226. PMID: 24176643, PMCID: PMC3834565, DOI: 10.1016/j.devcel.2013.09.021.Peer-Reviewed Original ResearchConceptsNeutrophil degranulationAcute innate immune responseIschemia-reperfusion injuryInnate immune responseProtection of kidneyNeutrophil functionImmune responseInhibition of exocytosisTissue damageGranule poolGranule contentsDegranulationImportant regulatorImportant roleVesicle exocytosisExocytosisSTK24InjuryNeutrophilsKidneyUNC13D
2012
Cytotoxic T lymphocyte effector function is independent of nucleus–centrosome dissociation
Lui‐Roberts W, Stinchcombe J, Ritter A, Akhmanova A, Karakesisoglou I, Griffiths G. Cytotoxic T lymphocyte effector function is independent of nucleus–centrosome dissociation. European Journal Of Immunology 2012, 42: 2132-2141. PMID: 22736282, PMCID: PMC3470926, DOI: 10.1002/eji.201242525.Peer-Reviewed Original ResearchConceptsCytotoxic T lymphocytesCentral supramolecular activation complexImmunological synapseCytotoxic T lymphocyte effector functionCytotoxic T lymphocyte cytotoxicityCytotoxic T lymphocyte killingT lymphocyte effector functionsSecretion of lytic granules contentSupramolecular activation complexVirally infected cellsLytic granule contentsT lymphocytesEffector functionsMock-treated cellsTarget cellsInfected cellsImmunofluorescence microscopyCell typesGranule contentsCellsSecretionRelease granulesSynapseNuclear membraneCentrosome docking
2002
Borrelia burgdorferi Are Susceptible to Killing by a Variety of Human Polymorphonuclear Leukocyte Components
Lusitani D, Malawista SE, Montgomery RR. Borrelia burgdorferi Are Susceptible to Killing by a Variety of Human Polymorphonuclear Leukocyte Components. The Journal Of Infectious Diseases 2002, 185: 797-804. PMID: 11920297, DOI: 10.1086/339341.Peer-Reviewed Original ResearchConceptsIntact polymorphonuclear leukocytesPolymorphonuclear leukocytesB. burgdorferiKilling of BorreliaHuman neutrophil peptides 1Neutrophil peptide-1Bactericidal/permeability-increasing proteinIntact human polymorphonuclear leukocytesHuman polymorphonuclear leukocytesPermeability-increasing proteinProteinase 3Peptide-1Leukocyte componentsNitric oxideLL-37Specific antibodiesBorrelia burgdorferiBurgdorferiGranule contentsEfficient killingSpirochetesCritical roleKillingLeukocytes
1998
Platelet function testing by flow cytometry.
Rinder H. Platelet function testing by flow cytometry. Clinical Laboratory Science 1998, 11: 365-72. PMID: 10345505.Peer-Reviewed Original ResearchConceptsFlow cytometryPlatelet functionEx vivo platelet functionVivo platelet functionPlatelet function testingIntracellular calcium fluxExposure of plateletsPlatelet glycoprotein receptorsFluorescent monoclonal antibodiesFunction testingPlatelet aggregationActivation statusCalcium fluxMonoclonal antibodiesMicroparticle generationCytometryGranule contentsCytometric methodologyPlateletsGlycoprotein receptorsSecretionAntibodiesReceptors
1996
Microbial killing by human neutrophil cytokineplasts: similar suppressive effects of reversible and irreversible inhibitors of nitric oxide synthase
Malawista S, Montgomery R, van Blaricom G. Microbial killing by human neutrophil cytokineplasts: similar suppressive effects of reversible and irreversible inhibitors of nitric oxide synthase. Journal Of Leukocyte Biology 1996, 60: 753-757. PMID: 8975878, DOI: 10.1002/jlb.60.6.753.Peer-Reviewed Original ResearchConceptsNO synthase inhibitorSynthase inhibitorL-NIOMin of incubationMonomethyl-L-arginineNitric oxide synthaseHuman blood neutrophilsSimilar suppressive effectReactive nitrogen intermediatesL-Arg analoguesIrreversible inhibitorBlood neutrophilsOxide synthaseSuppressive effectKilling capacityL-arginineKilling pathwaysHuman neutrophilsL-ArgD-ArgNeutrophilsNitrogen intermediatesMicrobial killingGranule contentsIntact neutrophils
1969
Vesicle and granule content of sympathetic ganglion cells during limb regeneration of the newt Triturus
Lentz T. Vesicle and granule content of sympathetic ganglion cells during limb regeneration of the newt Triturus. Cell And Tissue Research 1969, 102: 447-458. PMID: 4908503, DOI: 10.1007/bf00335487.Peer-Reviewed Original ResearchConceptsGanglion cellsNerve fibersNormal ganglion cellsSympathetic ganglion cellsPeripheral nerve fibersGranule contentsDense-core vesiclesNormal neuronsPeripheral nervesRegenerating fibersClear vesiclesNeuronsDense granulesMembrane-bounded granulesUpper forelimbNewt TriturusCellsLimb regenerationNerveTransectionAcid phosphatase activityDense contentGangliaVesicles increasesGranules
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