2025
Mineralocorticoid receptor phase separation modulates cardiac preservation
Lei I, Sicim H, Gao W, Huang W, Noly P, Pergande M, Wilson M, Lee A, Liu L, Abou El Ela A, Jiang M, Saddoughi S, Pober J, Platt J, Cascalho M, Pagani F, Chen Y, Pitt B, Wang Z, Mortensen R, Ge Y, Tang P. Mineralocorticoid receptor phase separation modulates cardiac preservation. Nature Cardiovascular Research 2025, 4: 710-726. PMID: 40389663, DOI: 10.1038/s44161-025-00653-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Cycle ProteinsCold IschemiaHeart TransplantationHistone Deacetylase 1HumansKidneyLiverLungMaleMiceMice, Inbred C57BLMineralocorticoid Receptor AntagonistsMyocytes, CardiacNuclear ProteinsOrgan PreservationPhase SeparationReceptors, MineralocorticoidTissue DonorsTranscription FactorsConceptsMineralocorticoid receptorDonor heartsHistone deacetylase 1Bromodomain-containing 4Gold standard treatmentEnd-stage heart failureCold ischemic timeShortage of donor heartsExpressed MRDonor cardiomyocytesHuman donor heartsHeart transplantationStandard treatmentIschemic timeHeart failureCardiac preservationSolid organsPharmacological inhibitionCold preservationPreserving biologyHeart
2022
Does utilization of heart machine perfusion for donation after cardiac death transplantation affect outcomes of other abdominal transplanted organs?
Okumura K, Misawa R, Ohira S, Dhand A, Kai M, Sogawa H, Veillette G, John D, Diflo T, Nishida S. Does utilization of heart machine perfusion for donation after cardiac death transplantation affect outcomes of other abdominal transplanted organs? Clinical Transplantation 2022, 36: e14751. PMID: 35706100, DOI: 10.1111/ctr.14751.Peer-Reviewed Original ResearchConceptsDelayed graft functionKidney transplantationUnited Network for Organ SharingOutcomes of liverGraft survivalMachine perfusionPropensity scoreDonor warm ischemic timeShort-term outcomesWarm ischemic timeDCD donorsPerfusion of heartsStatistically significant differenceGraft functionIschemic timeTransplantationPS matchingPerfusionSignificant differenceKidneyOrgan SharingLiverHeartCirculatory deathNo significant impact
2021
Trends and Outcomes of Cardiac Transplantation in the Lowest Urgency Candidates
Fuery MA, Chouairi F, Natov P, Bhinder J, Rose Chiravuri M, Wilson L, Clark KA, Reinhardt SW, Mullan C, Miller PE, Davis RP, Rogers JG, Patel CB, Sen S, Geirsson A, Anwer M, Desai N, Ahmad T. Trends and Outcomes of Cardiac Transplantation in the Lowest Urgency Candidates. Journal Of The American Heart Association 2021, 10: e023662. PMID: 34743559, PMCID: PMC9075266, DOI: 10.1161/jaha.121.023662.Peer-Reviewed Original ResearchConceptsLow-urgency patientsHigh-urgency patientsUrgency patientsCardiac transplantationAllocation system changeLonger waitlist timesOrgan Sharing databaseTime of transplantationCardiac transplantation candidatesNew allocation systemBaseline recipientPosttransplantation outcomesPosttransplantation survivalAdditional comorbiditiesClinical characteristicsConclusions PatientsHepatitis CIschemic timeTransplantation candidatesSharing databaseWaitlist timeWaitlist survivalUnited NetworkRetrospective analysisPatientsDifferential inflammatory responses of the native left and right ventricle associated with donor heart preservation
Lei I, Huang W, Ward PA, Pober JS, Tellides G, Ailawadi G, Pagani FD, Landstrom AP, Wang Z, Mortensen RM, Cascalho M, Platt J, Chen Y, Lam HYK, Tang PC. Differential inflammatory responses of the native left and right ventricle associated with donor heart preservation. Physiological Reports 2021, 9: e15004. PMID: 34435466, PMCID: PMC8387788, DOI: 10.14814/phy2.15004.Peer-Reviewed Original ResearchConceptsRight ventricleCold ischemiaIL-10Inflammatory responseIL-6 protein levelsCold ischemic preservationEx vivo ischemiaLeft ventricle dysfunctionCold ischemic timeDonor heart preservationInflammatory cytokine expressionCell deathDifferential inflammatory responseTumor necrosis factorComparable inflammatory responsesHuman donor heartsCaspase-3 expressionIschemic preservationVentricle dysfunctionInflammasome expressionIschemic timeRNA sequencingContractile dysfunctionDonor heartsWarm perfusionChanges in Use of Left Ventricular Assist Devices as Bridge to Transplantation With New Heart Allocation Policy
Mullan CW, Chouairi F, Sen S, Mori M, Clark KAA, Reinhardt SW, Miller PE, Fuery MA, Jacoby D, Maulion C, Anwer M, Geirsson A, Mulligan D, Formica R, Rogers JG, Desai NR, Ahmad T. Changes in Use of Left Ventricular Assist Devices as Bridge to Transplantation With New Heart Allocation Policy. JACC Heart Failure 2021, 9: 420-429. PMID: 33714748, DOI: 10.1016/j.jchf.2021.01.010.Peer-Reviewed Original ResearchConceptsDonor heart allocation systemNumber of patientsHeart allocation systemVentricular assist deviceWaitlist survivalAssist deviceNew heart allocation policyLeft ventricular assist deviceAllocation system changePost-transplant mortalityOrgan Sharing databaseOutcomes of patientsPost-transplantation outcomesProportion of patientsTime of transplantContinuous-flow LVADPost-transplantation survivalHeart allocation policyNew allocation systemBaseline recipientStatus 1AHeart transplantationIschemic timeLVAD implantationTransplant list
2020
A call to action: Feasible strategies to reduce the discard of transplantable kidneys in the United States
Stewart ZA, Shah SA, Formica RN, Kandaswamy R, Paramesh AS, Friedman J, Squires R, Cooper M, Axelrod DA. A call to action: Feasible strategies to reduce the discard of transplantable kidneys in the United States. Clinical Transplantation 2020, 34: e13990. PMID: 32621660, DOI: 10.1111/ctr.13990.Peer-Reviewed Original ResearchConceptsOrgan utilizationHigh-kidney donor profile index kidneysAcceptable post-transplant outcomesLow-risk organPost-transplant outcomesCold ischemic timeCold ischemia timeDeceased donor kidneysKidney allocation systemHigher transplant ratesNew allocation policyOrgan acceptanceIschemic timeDonor kidneysIschemia timeTransplant ratesOrgan discardRisk organsPractice patternsTransplantable kidneysClinical practicePathological interpretationGeographic sharingKidneyFinancial barriersA Data-Driven Approach to Defining the Volume-Outcome Relationship in Lung Transplantation
Godfrey E, Frankel W, Rana A, Liao K, Loor G, Suarez E. A Data-Driven Approach to Defining the Volume-Outcome Relationship in Lung Transplantation. The Journal Of Heart And Lung Transplantation 2020, 39: s364. DOI: 10.1016/j.healun.2020.01.445.Peer-Reviewed Original ResearchHigh-volume centersStratum-specific likelihood ratiosLung transplantationVolume centersVolume strataLung transplant centersOrgan Sharing databaseExtended criteria donorsVolume-outcome relationshipEx vivo perfusionMajority of transplantsLTx casesLTx centersLung transplantIschemic timeFinal cohortOlder recipientsPrimary outcomeSharing databaseSubstantial morbidityTransplant centersRecipient outcomesYear mortalityPatient survivalUnited NetworkChanges to the New United Network for Organ Sharing (UNOS) Heart Allocation System Did Not Affect Short Term Post-Transplant Mortality
Moonsamy P, Mohan N, Axtell A, Osho A, Ong C, Li S, D'Alessandro D, Sundt T, Funamoto M. Changes to the New United Network for Organ Sharing (UNOS) Heart Allocation System Did Not Affect Short Term Post-Transplant Mortality. The Journal Of Heart And Lung Transplantation 2020, 39: s73. DOI: 10.1016/j.healun.2020.01.1285.Peer-Reviewed Original ResearchExtracorporeal membrane oxygenationLeft ventricular assist devicePost-transplant mortalityNew allocation systemHeart transplantHeart allocation policyVentricular assist deviceUse of ECMOAssist deviceNew heart allocation policyWorse post-transplant outcomesIntra-aortic balloon pumpAdult heart transplantsOld allocation systemsPost-transplant deathPost-transplant outcomesShort-term outcomesShort-term mortalityHeart allocation systemNew United NetworkUNOS databaseIschemic timeOverall survivalPatient characteristicsBalloon pumpUtilization of New Heart Preservation Technology in the Era of New Organ Allocation and Extended Ischemic Times
Funamoto M, Ong C, Kilmarx S, Li S, Spencer P, Moonsamy P, Mohan N, Coglianese E, Lewis G, Pierson R, Villavicencio-Theoduloz M, D'Alessandro D. Utilization of New Heart Preservation Technology in the Era of New Organ Allocation and Extended Ischemic Times. The Journal Of Heart And Lung Transplantation 2020, 39: s136. DOI: 10.1016/j.healun.2020.01.1046.Peer-Reviewed Original ResearchPrimary graft dysfunctionLonger ischemic timesIschemic timeHeart transplantationOrgan allocationMethods Retrospective cohort analysisSevere primary graft dysfunctionExtended ischemic timesOlder recipient ageProlonged ischemic timePost-transplant outcomesRetrospective cohort analysisCold ischemic timeExtracorporeal membrane oxygenationMultiple organ transplantationGraft dysfunctionRecipient ageBaseline characteristicsAdult patientsConsecutive patientsClinical outcomesMembrane oxygenationOlder recipientsDonor heartsSingle institution
2019
Implementation of an experimental isolated lung perfusion model on surgically resected human lobes
Slama A, Raber C, Hedderich C, Stockhammer P, Hegedüs B, Koch A, Theegarten D, Ploenes T, Aigner C. Implementation of an experimental isolated lung perfusion model on surgically resected human lobes. Scientific Reports 2019, 9: 12193. PMID: 31434960, PMCID: PMC6704181, DOI: 10.1038/s41598-019-48719-8.Peer-Reviewed Original ResearchConceptsIsolated lung perfusionAnatomic pulmonary lobectomyLung perfusion modelExperimental lung researchDonor lungsIschemic timeMedian durationVariety of pathologiesPulmonary lobectomyLung perfusionMechanical ventilationArterial ligationLung cancerMetabolic parametersNormothermic conditionsRespiratory parametersPerfusion modelTreatment effectsLobePerfusionCancer researchLung researchVentilationPhysiological settingsAnimal organs
2018
Role of Class I and Class II Panel Reactive Antibodies in Predicting Post-Heart Transplant Rejection and Survival in the Current Era.
Kunnirickal S, Bellumkonda L. Role of Class I and Class II Panel Reactive Antibodies in Predicting Post-Heart Transplant Rejection and Survival in the Current Era. Journal Of Cardiac Failure 2018, 24: s112. DOI: 10.1016/j.cardfail.2018.07.414.Peer-Reviewed Original ResearchPanel reactive antibodyClass II antibodiesClass II panel reactive antibodiesClass I antibodiesYear of transplantationII antibodiesLong-term survivalClass IRecipient ageReactive antibodiesI antibodiesElevated panel reactive antibodyOverall long-term survivalLonger waitlist timesPrior heart transplantationLong-term mortalityMethods Retrospective analysisSame prognostic implicationsClass 2 antibodiesHeart transplantationHLA mismatchesHazard ratioIschemic timeHeart transplantPrognostic role
2016
A critical assessment on kidney allocation systems
Formica RN. A critical assessment on kidney allocation systems. Transplantation Reviews 2016, 31: 61-67. PMID: 27919635, DOI: 10.1016/j.trre.2016.10.002.Peer-Reviewed Original ResearchConceptsKidney allocation systemKidney allocationKidney Donor Profile IndexNew kidney allocation systemDelayed graft functionCold ischemic timeDeceased donor kidneysNumber of transplantsOrgan procurement organizationsGraft functionKidney transplantationIschemic timeDonor kidneysTransplant centersOrgan discard ratesClose monitoringMedical needKidneyProcurement organizationsPatientsTransplantationAllocation systemLife savingSignificant declineProfile indexChanges in Deceased Donor Kidney Transplantation One Year After KAS Implementation
Stewart DE, Kucheryavaya AY, Klassen DK, Turgeon NA, Formica RN, Aeder MI. Changes in Deceased Donor Kidney Transplantation One Year After KAS Implementation. American Journal Of Transplantation 2016, 16: 1834-1847. PMID: 26932731, DOI: 10.1111/ajt.13770.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedCadaverChildChild, PreschoolDelayed Graft FunctionDonor SelectionFemaleGovernment RegulationGraft SurvivalHealth Plan ImplementationHumansInfantInfant, NewbornKidney TransplantationMaleMiddle AgedRetrospective StudiesTime FactorsTissue and Organ ProcurementTissue DonorsUnited StatesYoung AdultConceptsKidney allocation systemDelayed graft function ratesNew kidney allocation systemGraft function rateGraft survival ratesCold ischemic timeKAS implementationLongevity matchingRecipient ageIschemic timeDonor kidneysSubsequent taperingFunction rateRecipient characteristicsOrgan procurementSurvival rateTransplantOne-yearBolus effectRecipientsKidneyDialysisExtended durationEarly impactYears
2014
Preanalytical variables and phosphoepitope expression in FFPE tissue: quantitative epitope assessment after variable cold ischemic time
Vassilakopoulou M, Parisi F, Siddiqui S, England AM, Zarella ER, Anagnostou V, Kluger Y, Hicks DG, Rimm DL, Neumeister VM. Preanalytical variables and phosphoepitope expression in FFPE tissue: quantitative epitope assessment after variable cold ischemic time. Laboratory Investigation 2014, 95: 334-341. PMID: 25418580, DOI: 10.1038/labinvest.2014.139.Peer-Reviewed Original ResearchConceptsCold ischemic timeIschemic timeFormalin fixationCompanion diagnostic testingCancer patientsBreast cancerTissue microarrayPhospho-HSP27Breast tumorsDiagnostic testingQuantitative immunofluorescenceAQUA technologyPreanalytical variablesGene expression profilingLoss of antigenicityTissue samplesReproducible assessmentProtein levelsConfidence intervalsSpecimen collectionExpression levelsPhosphorylation levelsAntigenicityRoutine usageResearch settingsA tissue quality index: an intrinsic control for measurement of effects of preanalytical variables on FFPE tissue
Neumeister VM, Parisi F, England AM, Siddiqui S, Anagnostou V, Zarrella E, Vassilakopolou M, Bai Y, Saylor S, Sapino A, Kluger Y, Hicks DG, Bussolati G, Kwei S, Rimm DL. A tissue quality index: an intrinsic control for measurement of effects of preanalytical variables on FFPE tissue. Laboratory Investigation 2014, 94: 467-474. PMID: 24535259, PMCID: PMC4030875, DOI: 10.1038/labinvest.2014.7.Peer-Reviewed Original ResearchConceptsCold ischemic timeIschemic timeQuantitative immunofluorescenceLevel of expressionTissue qualityER expression levelsBreast cancer casesExpression levelsFormalin fixationInformative epitopesValidation cohortCancer casesBreast cohortProtein statusGlobal assessmentPatient samplesTissue cohortPreanalytical variablesEpitope expressionCohortIntrinsic controlMeasurement of effectsQuality IndexFFPE tissuesEpitopes
2013
Cardiac transplantation can be safely performed using selected diabetic donors
Taghavi S, Jayarajan SN, Wilson LM, Komaroff E, Testani JM, Mangi AA. Cardiac transplantation can be safely performed using selected diabetic donors. Journal Of Thoracic And Cardiovascular Surgery 2013, 146: 442-447. PMID: 23490247, PMCID: PMC4067258, DOI: 10.1016/j.jtcvs.2013.02.047.Peer-Reviewed Original ResearchMeSH KeywordsAdultChi-Square DistributionDiabetes MellitusDonor SelectionFemaleHeart TransplantationHumansHypoglycemic AgentsInsulinKaplan-Meier EstimateMaleMiddle AgedMultivariate AnalysisProportional Hazards ModelsRegistriesRetrospective StudiesRisk AssessmentRisk FactorsTime FactorsTissue and Organ ProcurementTissue DonorsTreatment OutcomeUnited StatesConceptsExtracorporeal membrane oxygenationBody mass indexDiabetic donorsCardiac transplantationIschemic timeMultivariate analysisHuman leukocyte antigen mismatchesDonor body mass indexHigher Body Mass IndexRecipient black raceDuration of diabetesOrgan Sharing databaseInsulin-dependent diabetesAntigen mismatchesCardiac donorsRecipient creatinineRecipient diabetesMedian survivalMembrane oxygenationSharing databaseMass indexMechanical ventilationUnited NetworkRace mismatchBlack race
2012
Quantitative Assessment of Effect of Preanalytic Cold Ischemic Time on Protein Expression in Breast Cancer Tissues
Neumeister VM, Anagnostou V, Siddiqui S, England AM, Zarrella ER, Vassilakopoulou M, Parisi F, Kluger Y, Hicks DG, Rimm DL. Quantitative Assessment of Effect of Preanalytic Cold Ischemic Time on Protein Expression in Breast Cancer Tissues. Journal Of The National Cancer Institute 2012, 104: 1815-1824. PMID: 23090068, PMCID: PMC3514166, DOI: 10.1093/jnci/djs438.Peer-Reviewed Original ResearchMeSH KeywordsA Kinase Anchor ProteinsBiomarkers, TumorBiopsy, Large-Core NeedleBreast NeoplasmsCold IschemiaConfounding Factors, EpidemiologicFalse Negative ReactionsFemaleFixativesFluorescent Antibody TechniqueFormaldehydeGene Expression Regulation, NeoplasticHumansHypoxia-Inducible Factor 1, alpha SubunitKi-67 AntigenMastectomy, SegmentalMatched-Pair AnalysisMinor Histocompatibility AntigensProspective StudiesProto-Oncogene ProteinsReceptor, ErbB-2Receptors, EstrogenReceptors, ProgesteroneResearch DesignTime FactorsConceptsCold ischemic timeIschemic timeBreast cancer tissuesEstrogen receptorCancer tissuesLoss of antigenicityBreast cancer resectionProtein expressionCore needle biopsyCompanion diagnostic testsConditions of hypoxiaFalse-negative resultsBreast cancer biomarkersCancer resectionProgesterone receptorNeedle biopsyRecent guidelinesCold ischemiaBreast cancerTissue microarrayEvidence of lossQuantitative immunofluorescenceDiagnostic testsAntigenicityAQUA method
2011
P1-07-03: Preanalytical Variables Affect Protein Expression in Formalin Fixed Paraffin Embedded Tissue – Assessment of Intrinsic Controls To Define Tissue Quality for Immunohistochemical Analysis.
Neumeister V, Lostritto K, Siddiqui S, Anagnostou V, Vassilakopoulou M, Zarrella E, Molinaro A, Hicks D, Rimm D. P1-07-03: Preanalytical Variables Affect Protein Expression in Formalin Fixed Paraffin Embedded Tissue – Assessment of Intrinsic Controls To Define Tissue Quality for Immunohistochemical Analysis. Cancer Research 2011, 71: p1-07-03-p1-07-03. DOI: 10.1158/0008-5472.sabcs11-p1-07-03.Peer-Reviewed Original ResearchIschemic timeProtein expressionBreast cancerFormalin fixationDifferent breast cancer cohortsTumor tissuePairs of biopsiesTumor heterogeneityBreast cancer cohortBreast cancer biopsiesBreast cancer specimenPre-analytical variablesHistone 4ER alphaCancer cohortImmunohistochemical analysisCancer biopsiesSecond cohortAQUA scoreCancer specimenClinical settingQuantitative immunofluorescenceResectionCancer ResBiopsyQuantitative assessment shows loss of antigenic epitopes as a function of pre-analytic variables
Bai Y, Tolles J, Cheng H, Siddiqui S, Gopinath A, Pectasides E, Camp RL, Rimm DL, Molinaro AM. Quantitative assessment shows loss of antigenic epitopes as a function of pre-analytic variables. Laboratory Investigation 2011, 91: 1253-1261. PMID: 21519325, PMCID: PMC3145004, DOI: 10.1038/labinvest.2011.75.Peer-Reviewed Original ResearchConceptsCore needle biopsyCold ischemic timePre-analytic variablesNeedle biopsyEstrogen receptorIschemic timeTumor resectionTumor resection specimensAntigenic lossResection specimensTissue biomarkersTotal AktBiopsyPhospho-AktQuantitative immunofluorescencePhospho-ERKPhospho-S6K1Antigenic epitopesTotal ERKResectionTotal proteinCytokeratinImmunological methodsAntigenicitySignificant changesLiver and combined lung and liver transplantation for cystic fibrosis: Analysis of the UNOS database
Arnon R, Annunziato R, Miloh T, Padilla M, Sogawa H, Batemarco L, Willis A, Suchy F, Kerkar N. Liver and combined lung and liver transplantation for cystic fibrosis: Analysis of the UNOS database. Pediatric Transplantation 2011, 15: 254-264. PMID: 21219560, DOI: 10.1111/j.1399-3046.2010.01460.x.Peer-Reviewed Original ResearchConceptsIsolated LTLiver transplantationUNOS databaseRisk factorsProlonged cold ischemic timeEnd-stage liver diseaseOutcomes of LTOutcomes of patientsProportion of patientsCold ischemic timeLT patientsGraft lossGraft survivalPortal hypertensionCombined lungIschemic timeCystic fibrosisLiver diseasePulmonary diseasePatientsTransplantationLiverYoung adultsOutcomesGraft
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