2025
Cerebrospinal fluid and brain positron emission tomography measures of synaptic vesicle glycoprotein 2A: Biomarkers of synaptic density in Alzheimer's disease
Mecca A, Ashton N, Chen M, O'Dell R, Toyonaga T, Zhao W, Young J, Salardini E, Bates K, Ra J, Goodcase S, Silva‐Rudberg J, Nabulsi N, Brinkmalm A, Kvartsberg H, Schöll M, Nilsson J, Arnsten A, Huang Y, Hansson O, Zetterberg H, Carson R, Blennow K, van Dyck C. Cerebrospinal fluid and brain positron emission tomography measures of synaptic vesicle glycoprotein 2A: Biomarkers of synaptic density in Alzheimer's disease. Alzheimer's & Dementia 2025, 21: e70344. PMID: 40491249, PMCID: PMC12149441, DOI: 10.1002/alz.70344.Peer-Reviewed Original ResearchConceptsSynaptic vesicle glycoprotein 2APositron emission tomographyAlzheimer's diseaseSynaptic densityEnzyme-linked immunosorbent assayC]UCB-J positron emission tomographyPositron emission tomography measurementsEmission tomographyAxonal proteinsCN participantsImmunosorbent assaySymptomatic Alzheimer's diseaseAD groupProteinAssayParticipantsSV2AAlzheimerCerebrospinal fluidBrainInvestigate associationsCerebrospinal fluid assaysSV2A positron emission tomography
2024
The association between salivary oxytocin, age, and puberty in children with and without OCD
Mora-Jensen A, Clemmensen L, Grønberg M, Lebowitz E, Quintana D, Jørgensen N, Larsen C, Bak L, Christensen G, Pretzmann L, Uhre V, Christensen S, Uhre C, Korsbjerg N, Thoustrup C, Hagstrøm J, Ritter M, Plessen K, Pagsberg A, Lønfeldt N. The association between salivary oxytocin, age, and puberty in children with and without OCD. Scientific Reports 2024, 14: 28693. PMID: 39562644, PMCID: PMC11576962, DOI: 10.1038/s41598-024-80194-8.Peer-Reviewed Original ResearchConceptsObsessive-compulsive disorderOxytocin concentrationsPediatric obsessive-compulsive disorderSalivary oxytocin concentrationsVisual inspection of plotsOxytocin systemPsychiatric disordersSalivary oxytocinEnzyme-linked immunosorbent assayBehavioral changesPubertal stageAdolescentsModerate supportOxytocinInconsistent resultsPost-hocDisordersChildrenPost hoc testSystem activityCovariates ageParentsAssociationImmunosorbent assayHypothesisOptimized Enzyme-Linked Immunosorbent Assay for Anti-PEG Antibody Detection in Healthy Donors and Patients Treated with PEGylated Liposomal Doxorubicin
Li Y, Ettah U, Jacques S, Gaikwad H, Monte A, Dylla L, Guntupalli S, Moghimi S, Simberg D. Optimized Enzyme-Linked Immunosorbent Assay for Anti-PEG Antibody Detection in Healthy Donors and Patients Treated with PEGylated Liposomal Doxorubicin. Molecular Pharmaceutics 2024, 21: 3053-3060. PMID: 38743264, PMCID: PMC12109707, DOI: 10.1021/acs.molpharmaceut.4c00278.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayLiposomal doxorubicinHealthy donorsPatients treated with pegylated liposomal doxorubicinEnzyme-linked immunosorbent assay signalCancer patientsAnti-PEG IgGPegylated Liposomal DoxorubicinImmunosorbent assayOptimized ELISAEnzyme-linked immunosorbent assay protocolsAnti-PEG antibodiesChemotherapy cyclesBetween-assay variabilityPEGylated liposomesCutoff valueAccelerated clearanceTreatment cyclesDrug delivery systemsAntibody titersComplement activationInfused drugAnti-PEGOptimized assayPatientsThe development of lateral flow devices for urinary biomarkers to assess kidney health
D Souza S, Obeid W, Hernandez J, Hu D, Wen Y, Moledina D, Albert A, Gregg A, Wheeler A, Philbrook H, Parikh C. The development of lateral flow devices for urinary biomarkers to assess kidney health. Scientific Reports 2024, 14: 8516. PMID: 38609491, PMCID: PMC11014899, DOI: 10.1038/s41598-024-59104-5.Peer-Reviewed Original ResearchConceptsLateral flow deviceInterleukin-9Development of lateral flow devicesUrinary biomarkersDetection antibody concentrationSerum creatinine levelsKidney healthPaired antibodiesEnzyme Linked Immunosorbent AssayCreatinine levelsKidney injuryKidney functionOsteopontinImmunosorbent assayAntibody concentrationsMeasured biomarkersKidneyReal-time changesBiomarkersA comparison of chemiluminescent immunoassay and enzyme-linked immunosorbent assay for detecting phospholipase A2 receptor antibody in primary membranous nephropathy
Ma X, Wang R, Wei L, Liu P, Jing L, Wang J, Dong W, Tian X, Fu R. A comparison of chemiluminescent immunoassay and enzyme-linked immunosorbent assay for detecting phospholipase A2 receptor antibody in primary membranous nephropathy. Practical Laboratory Medicine 2024, 39: e00385. PMID: 38495349, PMCID: PMC10940994, DOI: 10.1016/j.plabm.2024.e00385.Peer-Reviewed Original ResearchAnti-PLA2R antibodiesEnzyme-linked immunosorbent assayNegative predictive valuePositive predictive valueAnti-PLA2RMembranous nephropathyChemiluminescence immunoassayLevels of anti-PLA2R antibodiesPhospholipase A2 receptor antibodyPredictive valuePrimary membranous nephropathyPhospholipase A2 receptorImmunosorbent assayResults Serum levelsStatistical analysis of sensitivityROC curve analysisNon-membranous nephropathyStatistically significant differencePMN groupReceptor antibodiesSerum levelsA2 receptorsSpearman correlation analysisDiagnostic valueNephropathy
2023
Seroprevalence Of SARS-COV-2 infection in asymptomatic indigenous from the largest Brazilian periurban area
de Oliveira L, Dos Santos Barbosa M, Leite Torres A, Croda M, Oliveira da Silva B, Dos Santos P, Rossoni R, Machado L, Croda J, Maymone Gonçalves C, Marques M, da Silva Ferreira T, Sardi S, Campos G, de Almeida G, Alves Gomes M, Marchioro S, Simionatto S. Seroprevalence Of SARS-COV-2 infection in asymptomatic indigenous from the largest Brazilian periurban area. PLOS ONE 2023, 18: e0295211. PMID: 38134187, PMCID: PMC10745159, DOI: 10.1371/journal.pone.0295211.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayCoronaVac inactivated vaccinesSARS-CoV-2SARS-CoV-2 infectionEnzyme-linked immunosorbent assay methodMedian ageSARS-CoV-2 antibodiesSeroprevalence of SARS-CoV-2 antibodiesSeroprevalence of SARS-CoV-2Asymptomatic individualsRapid testSeropositivity rateBlood samplesEthnic groupsElevated seroprevalenceImmunosorbent assaySeroprevalence of SARS-CoV-2 infectionSARS-CoV-2 rapid testSeropositivityAntibodiesVulnerable ethnic groupsSeroprevalence
2022
Association of circulating markers with cognitive decline after radiation therapy for brain metastasis.
Huntoon K, Anderson S, Ballman K, Twohy E, Dooley K, Jiang W, An Y, Li J, von Roemeling C, Qie Y, Ross O, Cerhan J, Whitton A, Greenspoon J, Parney I, Ashman J, Bahary J, Hadjipanayis C, Urbanic J, Farace E, Khuntia D, Laack N, Brown P, Roberge D, Kim B. Association of circulating markers with cognitive decline after radiation therapy for brain metastasis. Neuro-Oncology 2022, 25: 1123-1131. PMID: 36472389, PMCID: PMC10237411, DOI: 10.1093/neuonc/noac262.Peer-Reviewed Original ResearchConceptsWhole-brain radiation therapyStereotactic radiosurgeryBrain metastasesRadiation therapyNeurocognitive declineSerum levelsRadiation-induced neurocognitive declinePretreatment serum markersLow serum levelsAssociated with neurocognitive impairmentRandomized clinical trialsEnzyme-linked immunosorbent assaySurgical resectionSecondary endpointsSerum markersRetrospective analysisClinical trialsNeurocognitive impairmentCognitive impairmentMetastasisBlood samplesImmunosorbent assayMonthsSecondary analysisTherapyEnzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies
Larsen M, Bayarri-Olmos R, Garred P, Hviid L. Enzyme-Linked Immunosorbent Assay for Activation of the Classical Complement Pathway by Plasmodium falciparum-Infected Erythrocyte Surface Antigen-Specific Antibodies. Methods In Molecular Biology 2022, 2470: 673-678. PMID: 35881382, DOI: 10.1007/978-1-0716-2189-9_50.Peer-Reviewed Original ResearchConceptsClassical complement pathwayComplement pathwayAntigen-specific antibodiesInnate immune systemEnzyme-linked immunosorbentErythrocyte surface antigenAntibody titer determinationSurface antigenImmune systemComplement cascadeImmunosorbent assayMonoclonal antibodiesBiological cascadeAntibodiesTiter determinationActivationPathwayAntigenImmunosorbentELISAChanges Over Time in COVID-19 Incidence, Vaccinations, Serum Spike IgG, and Viral Neutralizing Potential Among Individuals From a North American Gaming Venue
Wisnewski AV, Cantley L, Luna J, Liu J, Smith RF, Hager K, Redlich CA. Changes Over Time in COVID-19 Incidence, Vaccinations, Serum Spike IgG, and Viral Neutralizing Potential Among Individuals From a North American Gaming Venue. Journal Of Occupational And Environmental Medicine 2022, 64: 788-796. PMID: 36054278, PMCID: PMC9426317, DOI: 10.1097/jom.0000000000002617.Peer-Reviewed Original ResearchConceptsOccupational risk factorsCOVID-19 historyRisk factorsAnti-spike IgG levelsIndividual humoral responsesSerum antiviral antibodiesCOVID-19 vaccinationEnzyme-linked immunosorbent assayCOVID-19 ratesCOVID-19 incidenceSpike IgGVaccine nonrespondersVaccination statusIgG levelsVaccine responsesVaccine typesAntiviral antibodiesHumoral responseCOVID-19 casesVaccine availabilityImmunosorbent assayVaccinationInfectionMonthsParticipantsPredominance of Severe Plasma Leakage in Pediatric Patients With Severe Dengue in Puerto Rico
Paz-Bailey G, Sánchez-González L, Torres-Velasquez B, Jones E, Perez-Padilla J, Sharp T, Lorenzi O, Delorey M, Munoz-Jordan J, Tomashek K, Waterman S, Alvarado L, Rivera-Amill V. Predominance of Severe Plasma Leakage in Pediatric Patients With Severe Dengue in Puerto Rico. The Journal Of Infectious Diseases 2022, 226: 1949-1958. PMID: 35510941, PMCID: PMC10015274, DOI: 10.1093/infdis/jiac165.Peer-Reviewed Original ResearchConceptsSevere plasma leakageSevere dengueReverse transcriptase-polymerase chain reactionPlasma leakageSevere bleedingDengue virusHistory of feverDays of presentationTranscriptase-polymerase chain reactionYear oldsNonsevere dengueOrgan involvementCommon presentationPediatric patientsClinical manifestationsDengue patientsEmergency departmentLaboratory findingsIgM enzymePatientsSerum samplesImmunosorbent assayDengueChain reactionBleedingLack of association between pandemic chilblains and SARS-CoV-2 infection
Gehlhausen JR, Little AJ, Ko CJ, Emmenegger M, Lucas C, Wong P, Klein J, Lu P, Mao T, Jaycox J, Wang E, Ugwu N, Muenker C, Mekael D, Klein R, Patrignelli R, Antaya R, McNiff J, Damsky W, Kamath K, Shon J, Ring A, Yildirim I, Omer S, Ko A, Aguzzi A, Iwasaki A, Obaid A, Lu-Culligan A, Nelson A, Brito A, Nunez A, Martin A, Watkins A, Geng B, Kalinich C, Harden C, Todeasa C, Jensen C, Kim D, McDonald D, Shepard D, Courchaine E, White E, Song E, Silva E, Kudo E, DeIuliis G, Rahming H, Park H, Matos I, Nouws J, Valdez J, Fauver J, Lim J, Rose K, Anastasio K, Brower K, Glick L, Sharma L, Sewanan L, Knaggs L, Minasyan M, Batsu M, Petrone M, Kuang M, Nakahata M, Campbell M, Linehan M, Askenase M, Simonov M, Smolgovsky M, Sonnert N, Naushad N, Vijayakumar P, Martinello R, Datta R, Handoko R, Bermejo S, Prophet S, Bickerton S, Velazquez S, Alpert T, Rice T, Khoury-Hanold W, Peng X, Yang Y, Cao Y, Strong Y. Lack of association between pandemic chilblains and SARS-CoV-2 infection. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2122090119. PMID: 35217624, PMCID: PMC8892496, DOI: 10.1073/pnas.2122090119.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionPrior SARS-CoV-2 infectionSARS-CoV-2PC biopsiesAcute respiratory syndrome coronavirus 2 pandemicSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemicT-cell receptor sequencingCell receptor sequencingT cell responsesCoronavirus 2 pandemicEnzyme-linked immunosorbent assayLack of associationCOVID toesSkin eruptionAntibody responseImmunohistochemistry studiesBackground seroprevalenceTissue microarrayViral infectionStimulation assaysCell responsesInfectionChilblainsImmunosorbent assayAbortive infection
2021
Antibodies to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in All of Us Research Program Participants, 2 January to 18 March 2020
Althoff K, Schlueter D, Anton-Culver H, Cherry J, Denny J, Thomsen I, Karlson E, Havers F, Cicek M, Thibodeau S, Pinto L, Lowy D, Malin B, Ohno-Machado L, Williams C, Goldstein D, Kouame A, Ramirez A, Roman A, Sharpless N, Gebo K, Schully S. Antibodies to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in All of Us Research Program Participants, 2 January to 18 March 2020. Clinical Infectious Diseases 2021, 74: 584-590. PMID: 34128970, PMCID: PMC8384413, DOI: 10.1093/cid/ciab519.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayBlood specimensSARS-CoV-2 enzyme-linked immunosorbent assaySARS-CoV-2 immunoglobulin G (IgG) antibodiesSARS-CoV-2 antibodiesIgG enzyme-linked immunosorbent assayConfidence intervalsImmunoglobulin G antibodiesSARS-CoV-2Sequential testing algorithmEUROIMMUN assaysSymptomatic patientsTesting algorithmStudy visitG antibodiesCommunity transmissionTravel historyStudy participantsUS epidemicEarly weeksImmunosorbent assayTesting capacityAntibodiesWeeksUS statesRisk of chronic arthralgia and impact of pain on daily activities in a cohort of patients with chikungunya virus infection from Brazil
Silva M, Kikuti M, Anjos R, Portilho M, Santos V, Gonçalves T, Tauro L, Moreira P, Jacob-Nascimento L, Santana P, Campos G, Siqueira A, Kitron U, Reis M, Ribeiro G. Risk of chronic arthralgia and impact of pain on daily activities in a cohort of patients with chikungunya virus infection from Brazil. International Journal Of Infectious Diseases 2021, 105: 608-616. PMID: 33684559, DOI: 10.1016/j.ijid.2021.03.003.Peer-Reviewed Original ResearchConceptsChronic arthralgiaChikungunya virus infectionRisk factorsVirus infectionDaily activitiesAcute febrile illnessIndependent risk factorPersistence of painCohort of patientsImpact of painProgression of diseaseTranscriptase-polymerase chain reactionPersistent arthralgiaFebrile illnessSubstantial disabilityViral loadFemale sexPolymerase chain reactionIgM enzymeArthralgiaSurveillance studyChikungunya virusDengue virusPatientsImmunosorbent assay
2020
Anti-Cancer Effects of RAW 264.7 Cells on Prostate Cancer PC-3 Cells.
Nam H, Bae J, Kim Y, An H, Kim S, Kim K, Yu S, Park B, Lee S, Ahn S. Anti-Cancer Effects of RAW 264.7 Cells on Prostate Cancer PC-3 Cells. Annals Of Clinical & Laboratory Science 2020, 50: 739-746. PMID: 33334788.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell CommunicationCell Line, TumorCell MovementCoculture TechniquesCulture Media, ConditionedEpithelial-Mesenchymal TransitionHumansImmunotherapy, AdoptiveLipopolysaccharidesMacrophagesMaleMiceNeoplasm InvasivenessPC-3 CellsProstatic NeoplasmsRAW 264.7 CellsTumor MicroenvironmentConceptsPC-3 cellsAnti-cancer effectsProstate cancer PC-3 cellsCancer PC-3 cellsRAW 264.7 cellsTumor cellsHuman prostate cancer PC-3 cellsEMT-specific markersHigher anti-cancer effectEnzyme-linked immunosorbent assayQuantitative polymerase chain reactionAnti-cancer agentsPolymerase chain reactionImmune cellsInhibitor of metastasisTumor parametersTherapeutic targetingTGF-β2Snail-1Mesenchymal transitionTumor microenvironmentMigration markersWestern blotImmunosorbent assayAngiogenic abilityAccuracy of the Zika IgM Antibody Capture Enzyme-Linked Immunosorbent Assay from the Centers for Disease Control and Prevention (CDC Zika MAC-ELISA) for Diagnosis of Zika Virus Infection
Portilho M, de Moraes L, Kikuti M, Nascimento L, Reis M, Boaventura V, Khouri R, Ribeiro G. Accuracy of the Zika IgM Antibody Capture Enzyme-Linked Immunosorbent Assay from the Centers for Disease Control and Prevention (CDC Zika MAC-ELISA) for Diagnosis of Zika Virus Infection. Diagnostics 2020, 10: 835. PMID: 33080935, PMCID: PMC7603149, DOI: 10.3390/diagnostics10100835.Peer-Reviewed Original ResearchDays post-symptom onsetZika virus infectionConvalescent-phase seraVirus infectionRT-PCRDengue virusSerological diagnosisDengue casesPost-symptom onsetConvalescent-phase samplesBlood donor samplesIgM antibody capture enzymeEnzyme-linked immunosorbent assayZika IgM antibodySymptom onsetDENV infectionIgM antibodiesZIKV infectionBlood donorsDonor samplesCapture enzymeOutbreak investigationDisease controlInfectionImmunosorbent assayEarly Increased Urinary IL-2 and IL-10 Levels Were Associated With Development of Chronic UTI in a Murine Model
Zhu K, Hill WG, Li F, Shi B, Chai TC. Early Increased Urinary IL-2 and IL-10 Levels Were Associated With Development of Chronic UTI in a Murine Model. Urology 2020, 141: 188.e1-188.e6. PMID: 32201154, DOI: 10.1016/j.urology.2020.03.015.Peer-Reviewed Original ResearchConceptsChronic urinary tract infectionUrinary tract infectionCytokines/chemokinesIL-2IL-10Enzyme-linked immunosorbent assayBacterial loadInflammatory cytokines/chemokinesTissue bacterial loadUrinary IL-2IL-10 levelsDays post infectionUTI susceptibilityTract infectionsMurine modelUTI infectionsPost infectionVoiding behaviorChemokinesMiceImmunosorbent assayResistant groupInfectionSignificant differencesDpi
2019
Discovery and Contribution of Nontypeable Haemophilus influenzae NTHI1441 to Human Respiratory Epithelial Cell Invasion
Ahearn C, Kirkham C, Chaves L, Kong Y, Pettigrew M, Murphy T. Discovery and Contribution of Nontypeable Haemophilus influenzae NTHI1441 to Human Respiratory Epithelial Cell Invasion. Infection And Immunity 2019, 87: 10.1128/iai.00462-19. PMID: 31427451, PMCID: PMC6803334, DOI: 10.1128/iai.00462-19.Peer-Reviewed Original ResearchConceptsChronic obstructive pulmonary diseaseRespiratory epithelial cellsEnzyme-linked immunosorbent assayEpithelial cellsAirways of adultsObstructive pulmonary diseaseCell invasionHuman respiratory epithelial cellsNontypeable Haemophilus influenzaeCell enzyme-linked immunosorbent assayAlveolar epithelial cellsAcute exacerbationLower airwaysPulmonary diseaseSerum IgGNTHi strainsTherapeutic interventionsHaemophilus influenzaeHuman bronchialSurface-exposed epitopesEpithelial cell invasionIsogenic knockout mutantsNTHiImmunosorbent assayIntracellular survival
2018
NEURO-EXERGAME: PILOT CLINICAL TRIAL OF AN IN-HOME INTERVENTION FOR MILD COGNITIVE IMPAIRMENT (IPACESV2.0)
Wall K, Stark J, Schillaci A, Saulnier E, McLaren E, Cohen B, Arciero P, Anderson-Hanley C. NEURO-EXERGAME: PILOT CLINICAL TRIAL OF AN IN-HOME INTERVENTION FOR MILD COGNITIVE IMPAIRMENT (IPACESV2.0). Innovation In Aging 2018, 2: 984-985. PMCID: PMC6239807, DOI: 10.1093/geroni/igy031.3642.Peer-Reviewed Original ResearchMild cognitive impairmentEnzyme-linked immunosorbent assayCognitive impairmentCognitive declinePilot clinical trialThree-month interventionExecutive functionTreat analysisDementia casesBiomarker levelsClinical trialsIGF-1Improved cognitionEfficacious interventionsPhysical exerciseSalivary biomarkersHome interventionSaliva samplesImmunosorbent assayCognitive outcomesWeek intervalsPilot dataBmGPAC, an Antigen Capture Assay for Detection of Active Babesia microti Infection
Thekkiniath J, Mootien S, Lawres L, Perrin BA, Gewirtz M, Krause PJ, Williams S, Doggett J, Ledizet M, Mamoun C. BmGPAC, an Antigen Capture Assay for Detection of Active Babesia microti Infection. Journal Of Clinical Microbiology 2018, 56: 10.1128/jcm.00067-18. PMID: 30093394, PMCID: PMC6156295, DOI: 10.1128/jcm.00067-18.Peer-Reviewed Original ResearchConceptsHuman babesiosisBabesia microti infectionCapture enzyme-linked immunosorbent assayAntigen capture enzyme-linked immunosorbent assayAntigen capture assayEnzyme-linked immunosorbent assayZoonotic infectious diseaseAcute infectionBlood transfusionAsymptomatic infectionMicroti infectionReal-time PCRBlood supplyAnimal reservoir hostsDonor bloodEpidemiological surveyHuman patientsImmune systemSerological assaysImmunodominant antigensInfectionInfectious diseasesIntraerythrocytic protozoan parasitePatientsImmunosorbent assayTrypanosoma cruzi Produces the Specialized Proresolving Mediators Resolvin D1, Resolvin D5, and Resolvin E2
Colas RA, Ashton AW, Mukherjee S, Dalli J, Akide-Ndunge OB, Huang H, Desruisseaux MS, Guan F, Jelicks LA, dos Santos F, Nagajyothi J, Zingman MA, Reyes J, Weiss LM, Serhan CN, Tanowitz HB. Trypanosoma cruzi Produces the Specialized Proresolving Mediators Resolvin D1, Resolvin D5, and Resolvin E2. Infection And Immunity 2018, 86: 10.1128/iai.00688-17. PMID: 29358332, PMCID: PMC5865043, DOI: 10.1128/iai.00688-17.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayResolvin D1Chagas diseaseMediators of inflammationCD-1 miceRvD1 levelsChronic cardiomyopathyAcute myocarditisMyocardial inflammationProinflammatory eicosanoidsProresolving mediatorsChronic phasePathological featuresPlasma levelsLifelong infectionLipid mediatorsResolvin D5Host responseHuman infectionsBacterial infectionsRvD1InflammationInfectionImmunosorbent assayElevated levels
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