Micha Sam Brickman Raredon, MD, PhD
Assistant Professor of AnesthesiologyCards
About
Research
Overview
Dr. Raredon's research group focuses on the mechanisms governing the behavior of complex multicellular systems and in leveraging these principles to engineer living tissues for regenerative medicine. Our work combines systems biology, stem-cell engineering, materials science, surgical fabrication, and biological engineering. The team is particularly invested in engineering, or controlling the self-organization of, cross-length scale perfusable vascular networks and tissue architecture, and in devising principles of microvascular construction and control that can be applied across organ types. We are a clinically-integrated laboratory with two broadly interconnected goals: the modeling of network-level mechanisms of tissue morphogenesis and phenotype regulation, and the application of those models to the design and fabrication of patient-specific tissues for therapeutic use.
Publications
2024
Single-Cell Analysis Reveals Novel Immune Perturbations in Fibrotic Hypersensitivity Pneumonitis.
Zhao A, Unterman A, Abu Hussein N, Sharma P, Nikola F, Flint J, Yan X, Adams T, Justet A, Sumida T, Zhao J, Schupp J, Raredon M, Ahangari F, Deluliis G, Zhang Y, Buendia-Roldan I, Adegunsoye A, Sperling A, Prasse A, Ryu C, Herzog E, Selman M, Pardo A, Kaminski N. Single-Cell Analysis Reveals Novel Immune Perturbations in Fibrotic Hypersensitivity Pneumonitis. American Journal Of Respiratory And Critical Care Medicine 2024, 210: 1252-1266. PMID: 38924775, PMCID: PMC11568434, DOI: 10.1164/rccm.202401-0078oc.Peer-Reviewed Original ResearchFibrotic hypersensitivity pneumonitisIdiopathic pulmonary fibrosisPeripheral blood mononuclear cellsBronchoalveolar lavage cellsBlood mononuclear cellsClassical monocytesHypersensitivity pneumonitisPulmonary fibrosisT cellsImmune perturbationsLavage cellsMononuclear cellsCD8+ T cellsCytotoxic T cellsInterstitial lung diseaseHypersensitivity pneumonitis patientsCytotoxic CD4Immune aberrationsPneumonic patientsPneumonitisLung diseaseHealthy controlsImmune mechanismsPatient cellsSingle-cell transcriptomicsSingle-cell RNA-seq analysis of cell-cell communications in human lung reveals a novel role of VEGF-D in acute lung injury
Yuan Y, Sharma L, Tang W, Raredon M, Ahangari F, Khoury J, Wu D, Niklason L, Kaminski N. Single-cell RNA-seq analysis of cell-cell communications in human lung reveals a novel role of VEGF-D in acute lung injury. Physiology 2024, 39: 1314. DOI: 10.1152/physiol.2024.39.s1.1314.Peer-Reviewed Original ResearchIdiopathic pulmonary fibrosisAcute lung injuryChronic obstructive pulmonary diseaseAcute respiratory distress syndromeAnalysis of cell-cell communicationVEGF-DMicrovascular nicheSingle-cell RNA-seqLung injury modelSingle-cell RNA-seq analysisLung injuryCell-cell communicationLigand-receptor pairsLPS-induced lung injury modelRNA-seqAdjacent cell typesPulmonary diseaseInjury modelHuman lung endothelial cellsBarrier functionImmune cell infiltrationTumor necrosis factor-aRespiratory distress syndromeLung vascular integrityGene expressionVEGF-D Is Enriched in the Human Lung Microvascular Niche and Improves Vascular Integrity During Acute Lung Injury
Yuan Y, Sharma L, Tang W, Raredon M, Ahangari F, Khoury J, Wu D, Niklason L, Kaminski N. VEGF-D Is Enriched in the Human Lung Microvascular Niche and Improves Vascular Integrity During Acute Lung Injury. 2024, a4694-a4694. DOI: 10.1164/ajrccm-conference.2024.209.1_meetingabstracts.a4694.Peer-Reviewed Original ResearchAberrant Alveolar Epithelial Cells Alter the Alveolar Mesenchyme to Promote Fibrosis in a Clinical Sftpc Mutation Model of Lung Fibrosis
Hoffman E, Roque Barboza W, Rodriguez L, Murthy A, Dherwani R, Bennett A, Tomer Y, Raredon M, Beers M, Katzen J. Aberrant Alveolar Epithelial Cells Alter the Alveolar Mesenchyme to Promote Fibrosis in a Clinical Sftpc Mutation Model of Lung Fibrosis. 2024, a5197-a5197. DOI: 10.1164/ajrccm-conference.2024.209.1_meetingabstracts.a5197.Peer-Reviewed Original Research
2023
Rational engineering of lung alveolar epithelium
Leiby K, Yuan Y, Ng R, Raredon M, Adams T, Baevova P, Greaney A, Hirschi K, Campbell S, Kaminski N, Herzog E, Niklason L. Rational engineering of lung alveolar epithelium. Npj Regenerative Medicine 2023, 8: 22. PMID: 37117221, PMCID: PMC10147714, DOI: 10.1038/s41536-023-00295-2.Peer-Reviewed Original ResearchSARS-CoV-2 leverages airway epithelial protective mechanism for viral infection
Greaney A, Raredon M, Kochugaeva M, Niklason L, Levchenko A. SARS-CoV-2 leverages airway epithelial protective mechanism for viral infection. IScience 2023, 26: 106175. PMID: 36788793, PMCID: PMC9912025, DOI: 10.1016/j.isci.2023.106175.Peer-Reviewed Original ResearchSingle-cell RNA sequencing datasetsCell-cell communicationRNA sequencing datasetsViral infectionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral infectionEarly SARS-CoV-2 infectionSequencing datasetsSARS-CoV-2 infectionRepair mechanismsEarly viral entryTissue repair mechanismsMature ciliated cellsAlternative therapeutic approachSARS-CoV-2 virusResponse mechanismsFeedforward loopCell precursorsRapid differentiationViral entryBronchial epitheliumTherapeutic approachesBarrier tissuesKey mechanismCiliated cellsInfection
2022
Comprehensive visualization of cell–cell interactions in single-cell and spatial transcriptomics with NICHES
Raredon M, Yang J, Kothapalli N, Lewis W, Kaminski N, Niklason L, Kluger Y. Comprehensive visualization of cell–cell interactions in single-cell and spatial transcriptomics with NICHES. Bioinformatics 2022, 39: btac775. PMID: 36458905, PMCID: PMC9825783, DOI: 10.1093/bioinformatics/btac775.Peer-Reviewed Original ResearchConceptsCell-cell interactionsCell-cell signalingSingle-cell resolutionSingle-cell dataLocal cellular microenvironmentSingle-cell levelSpatial transcriptomics dataCell clustersExtracellular signalingTranscriptomic dataGene expression valuesSpatial transcriptomicsSignaling mechanismCellular microenvironmentNicheExpression valuesSupplementary dataSignalingTranscriptomicsComprehensive visualizationBioinformaticsInteractionFibroblast inflammatory priming determines regenerative versus fibrotic skin repair in reindeer
Sinha S, Sparks H, Labit E, Robbins H, Gowing K, Jaffer A, Kutluberk E, Arora R, Raredon M, Cao L, Swanson S, Jiang P, Hee O, Pope H, Workentine M, Todkar K, Sharma N, Bharadia S, Chockalingam K, de Almeida L, Adam M, Niklason L, Potter S, Seifert A, Dufour A, Gabriel V, Rosin N, Stewart R, Muench G, McCorkell R, Matyas J, Biernaskie J. Fibroblast inflammatory priming determines regenerative versus fibrotic skin repair in reindeer. Cell 2022, 185: 4717-4736.e25. PMID: 36493752, PMCID: PMC9888357, DOI: 10.1016/j.cell.2022.11.004.Peer-Reviewed Original ResearchConceptsFibrotic scarSite-specific immune responseFull-thickness injurySkin fibroblastsWound healing outcomesInflammatory mediatorsImmunosuppressive phenotypeImmune resolutionLeukocyte recruitmentMyeloid infiltrationImmune responseHuman fetal fibroblastsHealing outcomesFibrotic phenotypeBack skinEctopic transplantationAdult humansSkin woundsScarSkinSkin repairPowerful comparative modelFibroblastsFetal fibroblastsRepairSoluble Signals to Improve Endothelial Integrity in the Lung
Yuan Y, Raredon M, Yuan Q, Obata T, Qian H, Wu D, Kaminski N, Niklason L. Soluble Signals to Improve Endothelial Integrity in the Lung. 2022, a5749-a5749. DOI: 10.1164/ajrccm-conference.2022.205.1_meetingabstracts.a5749.Peer-Reviewed Original ResearchComputation and visualization of cell–cell signaling topologies in single-cell systems data using Connectome
Raredon MSB, Yang J, Garritano J, Wang M, Kushnir D, Schupp JC, Adams TS, Greaney AM, Leiby KL, Kaminski N, Kluger Y, Levchenko A, Niklason LE. Computation and visualization of cell–cell signaling topologies in single-cell systems data using Connectome. Scientific Reports 2022, 12: 4187. PMID: 35264704, PMCID: PMC8906120, DOI: 10.1038/s41598-022-07959-x.Peer-Reviewed Original Research
Academic Achievements & Community Involvement
News & Links
Media
- Photo by Micha Sam Brickman Raredon
Immunostaining of cellular communities at the bronchioalveolar junction in lung tissue
News
- May 19, 2022
A Celebration of Student Research and Faculty Mentorship
- April 16, 2020
Sharing a Ventilator, Sparing a Life: Two Yale Groups Create Different Ways To Maximize Ventilator Capacity in a Crisis
- April 09, 2020Source: Yale School of Engineering and Applied Sciences
With an Urgent Need, Researchers Develop Multi-Patient Ventilators
- December 03, 2019
With Cellular Blueprint for Lungs, Yale Researchers Look Ahead to Organ Regeneration