Ruoyi Jiang, MD, PhD
Clinical Fellow T32About
Research
Publications
2023
High-throughput single-cell profiling of B cell responses following inactivated influenza vaccination in young and older adults
Wang M, Jiang R, Mohanty S, Meng H, Shaw A, Kleinstein S. High-throughput single-cell profiling of B cell responses following inactivated influenza vaccination in young and older adults. Aging 2023, 15: 9250-9274. PMID: 37367734, PMCID: PMC10564424, DOI: 10.18632/aging.204778.Peer-Reviewed Original ResearchConceptsB cellsactivated B cellsB cell receptorolder adultsinfluenza vaccinationage groupsperipheral blood B cellsyoung adultsinactivated influenza vaccineB cell responsessubstantial disease burdenblood B cellsmemory B cellsinfluenza vaccination responsesstrong antibody responseage-related changesinfluenza vaccinevaccination responseseasonal influenzaantibody responsehospital visitsdisease burdensomatic hypermutation frequenciesvaccinationcell responses
2021
Intranasal priming induces local lung-resident B cell populations that secrete protective mucosal antiviral IgA
Oh JE, Song E, Moriyama M, Wong P, Zhang S, Jiang R, Strohmeier S, Kleinstein SH, Krammer F, Iwasaki A. Intranasal priming induces local lung-resident B cell populations that secrete protective mucosal antiviral IgA. Science Immunology 2021, 6: eabj5129-eabj5129. PMID: 34890255, PMCID: PMC8762609, DOI: 10.1126/sciimmunol.abj5129.Peer-Reviewed Original ResearchConceptsvirus infectionIgA secretionB cellsmucosal surfacesIgA-secreting B cellsIgA-expressing cellsrole of IgArespiratory virus infectionsIgA-secreting cellslower respiratory tractinfluenza virus infectioneffective immune protectionheterologous virus infectionmemory B cellssecretory immunoglobulin Aprotein-based vaccinesB cell populationspredominant Ig isotypesite of entryintranasal primingbronchoalveolar spaceprotective immunityvaccine strategiesrespiratory mucosaimmune protectionElevated N-linked glycosylation of IgG variable regions in myasthenia gravis disease subtypes
Mandel-Brehm C, Fichtner ML, Jiang R, Winton VJ, Vazquez SE, Pham MC, Hoehn KB, Kelleher NL, Nowak RJ, Kleinstein SH, Wilson MR, DeRisi JL, O’Connor KC. Elevated N-linked glycosylation of IgG variable regions in myasthenia gravis disease subtypes. The Journal Of Immunology 2021, 207: 2005-2014. PMID: 34544801, PMCID: PMC8492536, DOI: 10.4049/jimmunol.2100225.Peer-Reviewed Original ResearchConceptsmyasthenia gravisB-cell-mediated autoimmune diseasesBCR repertoirecell-mediated autoimmune diseasetotal BCR repertoiretotal circulating IgGsubset of patientsB cell repertoireElevated Ngene segment usageMG subtypesautoimmune disordersautoimmune diseaseshealthy donorscell repertoiredisease subtypesdistinct subtypesreceptor repertoireadaptive immune receptor repertoiresV regionsautoantigen bindingpatientssegment usagesubtypesimmune receptor repertoiresRisk factor identification and predictive models for central line requirements for patients on vasopressors
Haimovich AD, Jiang R, Taylor RA, Belsky JB. Risk factor identification and predictive models for central line requirements for patients on vasopressors. Anaesthesia And Intensive Care 2021, 49: 275-283. PMID: 34392707, DOI: 10.1177/0310057x211024258.Peer-Reviewed Original ResearchConceptscentral venous cathetersintensive care unitvenous catheterscomposite endpointcare unitlogistic regression modelscatheter requirementrisk factorsintensive care unit settingfuture prospective trialsimportant risk factorstudy inclusion criteriaMedical Information Martrisk factor identificationmore vasopressorsmultiple vasopressorssingle vasopressorrenal impairmentperipheral administrationprospective trialregression modelsretrospective studyintensive careunit settinghigh prevalenceSingle cell immunophenotyping of the skin lesion erythema migrans Identifies IgM memory B cells
Jiang R, Meng H, Raddassi K, Fleming I, Hoehn KB, Dardick KR, Belperron AA, Montgomery RR, Shalek AK, Hafler DA, Kleinstein SH, Bockenstedt LK. Single cell immunophenotyping of the skin lesion erythema migrans Identifies IgM memory B cells. JCI Insight 2021, 6: e148035. PMID: 34061047, PMCID: PMC8262471, DOI: 10.1172/jci.insight.148035.Peer-Reviewed Original ResearchConceptsmemory B cellserythema migransB cellsEM lesionsIgM memory B cellsLyme diseaseB-cell receptor sequencingskin infection sitecell receptor sequencingearly Lyme diseaselocal antigen presentationskin immune responsesB cell populationssingle-cell immunophenotypingMHC class II genesuninvolved skinimmune cellsspirochetal infectionantigen presentationcell immunophenotypingT cellsimmune responseisotype usageantibody productioninitial signsDivergent and self-reactive immune responses in the CNS of COVID-19 patients with neurological symptoms
Song E, Bartley CM, Chow RD, Ngo TT, Jiang R, Zamecnik CR, Dandekar R, Loudermilk RP, Dai Y, Liu F, Sunshine S, Liu J, Wu W, Hawes IA, Alvarenga BD, Huynh T, McAlpine L, Rahman NT, Geng B, Chiarella J, Goldman-Israelow B, Vogels CBF, Grubaugh ND, Casanovas-Massana A, Phinney BS, Salemi M, Alexander JR, Gallego JA, Lencz T, Walsh H, Wapniarski AE, Mohanty S, Lucas C, Klein J, Mao T, Oh J, Ring A, Spudich S, Ko AI, Kleinstein SH, Pak J, DeRisi JL, Iwasaki A, Pleasure SJ, Wilson MR, Farhadian SF. Divergent and self-reactive immune responses in the CNS of COVID-19 patients with neurological symptoms. Cell Reports Medicine 2021, 2: 100288. PMID: 33969321, PMCID: PMC8091032, DOI: 10.1016/j.xcrm.2021.100288.Peer-Reviewed Original Researchneurological symptomsimmune responsecerebrospinal fluidanti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodiesCOVID-19self-reactive immune responsesSARS-CoV-2 antibodiescompartmentalized immune responseCSF immunoglobulin Grole of autoimmunityCOVID-19 patientsB cell responsescoronavirus disease 2019immune surveyneurologic sequelaepulmonary infectionbrain infectionserum antibodiesdisease 2019monoclonal antibody targetsanimal modelstarget epitopescell activationcell responsessingle-cell RNA sequencing
2020
Thymus-derived B cell clones persist in the circulation after thymectomy in myasthenia gravis
Jiang R, Hoehn KB, Lee CS, Pham MC, Homer RJ, Detterbeck FC, Aban I, Jacobson L, Vincent A, Nowak RJ, Kaminski HJ, Kleinstein SH, O'Connor KC. Thymus-derived B cell clones persist in the circulation after thymectomy in myasthenia gravis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 30649-30660. PMID: 33199596, PMCID: PMC7720237, DOI: 10.1073/pnas.2007206117.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAutoantibodiesB-LymphocytesBiomarkersClonal EvolutionClonal Selection, Antigen-MediatedDisease SusceptibilityFemaleHumansLymphocyte CountMaleMiddle AgedModels, BiologicalMyasthenia GravisRadioimmunoassayReceptors, CholinergicThymectomyThymus GlandV(D)J RecombinationYoung AdultConceptsB cell clonesmyasthenia gravisB cell repertoireB cellscell clonesplasma cellscell repertoireadditional immunosuppressive treatmentdiminished clinical responsethymic lymphofollicular hyperplasiacomplete stable remissionmajority of patientsantigen-experienced B cellsrandomized clinical trialsclinical symptom measuresAChR autoantibodiesimmunosuppressive treatmentsteroid doseautoantibody titersMG thymusclinical responsestable remissionclinical scoresautoimmune diseasesclinical trialsAffinity maturation is required for pathogenic monovalent IgG4 autoantibody development in myasthenia gravis
Fichtner ML, Vieni C, Redler RL, Kolich L, Jiang R, Takata K, Stathopoulos P, Suarez PA, Nowak RJ, Burden SJ, Ekiert DC, O’Connor K. Affinity maturation is required for pathogenic monovalent IgG4 autoantibody development in myasthenia gravis. Journal Of Experimental Medicine 2020, 217: e20200513. PMID: 32820331, PMCID: PMC7953735, DOI: 10.1084/jem.20200513.Peer-Reviewed Original ResearchConceptsmyasthenia gravisunmutated common ancestorpathogenic capacitymuscle-specific tyrosine kinaseaffinity maturationMuSK myasthenia gravisautoimmune myasthenia gravismonovalent antigen-binding fragmentsunique autoantibodiesIgG4 autoantibodiesautoantibody developmentautoantibodiesFab-arm exchangemonoclonal autoantibodiesantigen-binding fragmentsgravissomatic mutationssubnanomolar affinitymAbsmonovalent Fabtyrosine kinasematurationimmunopathologyautoimmunityHigh-throughput investigation of molecular and cellular biomarkers in NMOSD
Yandamuri SS, Jiang R, Sharma A, Cotzomi E, Zografou C, Ma AK, Alvey JS, Cook LJ, Smith TJ, Yeaman MR, O'Connor KC. High-throughput investigation of molecular and cellular biomarkers in NMOSD. Neurology Neuroimmunology & Neuroinflammation 2020, 7: e852. PMID: 32753407, PMCID: PMC7413712, DOI: 10.1212/nxi.0000000000000852.Peer-Reviewed Original ResearchConceptsneuromyelitis optica spectrum disorderperipheral blood mononuclear cellshealthy controlsNK cellscell subsetsT cellsunstimulated peripheral blood mononuclear cellsB-cell activating factordendritic cell subsetsoptica spectrum disorderregulatory T cellsnatural killer cellsimmune cell subsetsmultivariable logistic regressionblood mononuclear cellsserum analyte levelsmultivariable linear regressionmultivariable regression modelsnovel biomarker candidatesCX3CL1 levelsNMOSD cohortserum cytokinesdisease relapsekiller cellsserum cohortSingle-cell repertoire tracing identifies rituximab-resistant B cells during myasthenia gravis relapses
Jiang R, Fichtner ML, Hoehn KB, Pham MC, Stathopoulos P, Nowak RJ, Kleinstein SH, O'Connor KC. Single-cell repertoire tracing identifies rituximab-resistant B cells during myasthenia gravis relapses. JCI Insight 2020, 5 PMID: 32573488, PMCID: PMC7453893, DOI: 10.1172/jci.insight.136471.Peer-Reviewed Original ResearchConceptsmuscle-specific kinase myasthenia gravismemory B cellsB cell subsetsantibody-secreting cellsB cellscell subsetsautoantibody-producing B cellsB-cell depleting therapycell-depleting therapyB cell clonesB cell survivalgene expression signaturesmyasthenia gravisautoimmune disordersrelapseB cell samplesreceptor profilingcell clonesexpression signaturesrituximabtherapycell survivalcellstreatmentcell samples