2021
Deletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse
Thomson R, Dynia DW, Burlein S, Thomson BR, Booth C, Knauf F, Wang T, Aronson P. Deletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse. American Journal Of Physiology. Renal Physiology 2021, 320: f1106-f1122. PMID: 33938239, PMCID: PMC8285649, DOI: 10.1152/ajprenal.00556.2020.Peer-Reviewed Original ResearchConceptsKsp-cadherinCell adhesion moleculeAtypical memberKidney developmentMammalian kidneyAdult mammalian kidneyBasolateral membraneNormal kidney developmentEpithelial cellsAdhesion moleculesMutant animalsExpression analysisSpecific expressionE-cadherin expressionWestern blot analysisEpithelial phenotypePrincipal proteinE-cadherinBlot analysisMouse linesAquaporin-2CadherinCritical roleDevelopmental delayKnockout mice
2019
Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice
Li J, Xu S, Yang L, Yang J, Wang CJ, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice. American Journal Of Physiology. Renal Physiology 2019, 317: f967-f977. PMID: 31390232, PMCID: PMC6843050, DOI: 10.1152/ajprenal.00125.2019.Peer-Reviewed Original ResearchConceptsHK dietCation excretionWT animalsFemale animalsMale animalsSex differencesRenal clearance experimentsGlomerular filtration rateR KO miceExchanger isoform 3Distal NaHK intakeWestern blot analysisFractional excretionUrine volumeFiltration rateFemale miceKO miceNHE3 abundanceClearance experimentsNCC abundanceLittle sex differenceKnockout miceENaC expressionExcretion
2010
Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells
Duan Y, Weinstein A, Weinbaum S, Wang T. Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 21860-21865. PMID: 21106755, PMCID: PMC3003103, DOI: 10.1073/pnas.1015751107.Peer-Reviewed Original ResearchConceptsProximal tubule cellsMouse proximal tubuleNa/K-ATPaseTubule cellsApical NHE3Proximal tubulesMouse proximal tubule cellsFSS-induced changesApical plasma membraneV-ATPaseAmount of protein expressionBasolateral membraneWestern blot analysisNHE3Volume reabsorptionTransporter expressionBasolateral transportProtein expressionMPT cellsShear stress-induced changesVacuolar H(+)-ATPaseBlock microtubule polymerizationControl cellsModulate cellular functionsTransporter localization
2003
Inhibition of heme oxygenase decreases sodium and fluid absorption in the loop of Henle
Wang T, Sterling H, Shao W, Yan Q, Bailey M, Giebisch G, Wang W. Inhibition of heme oxygenase decreases sodium and fluid absorption in the loop of Henle. American Journal Of Physiology. Renal Physiology 2003, 285: f484-f490. PMID: 12890663, DOI: 10.1152/ajprenal.00135.2003.Peer-Reviewed Original ResearchConceptsLoop of HenleHigh K+K+ channelsApical 70-pS K+ channelRegulation of Na+ transportNa+ transportFluid absorptionApical K+ channelsIn vivo microperfusionInhibition of HORenal sodium excretionGlomerular filtration rateHO-2 expressionInhibitor of HORenal clearance studiesHK ratsHO-2K+ recyclingNa+ absorptionUrinary Na+Sodium excretionChromium mesoporphyrinRat chowWestern blot analysisFiltration rateIntracellular Na concentration and Rb uptake in proximal convoluted tubule cells and abundance of Na/K-ATPase α1-subunit in NHE3−/− mice
Beck F, Neuhofer W, Dörge A, Giebisch G, Wang T. Intracellular Na concentration and Rb uptake in proximal convoluted tubule cells and abundance of Na/K-ATPase α1-subunit in NHE3−/− mice. Pflügers Archiv - European Journal Of Physiology 2003, 446: 100-105. PMID: 12690468, DOI: 10.1007/s00424-002-1001-z.Peer-Reviewed Original ResearchConceptsNhe3-/- miceCell Na concentrationIntracellular Na concentrationProximal convoluted tubulesNhe3-/-Proximal convoluted tubule cellsNa/K-ATPaseNa/H exchanger isoform 3Rb uptakeConvoluted tubulesExpression of Na/K-ATPaseApical Na entryExchanger isoform 3Intracellular Cl- concentrationNa/K-ATPase activityDistal convoluted tubuleConvoluted tubule cellsApical NHE3NHE3 deficiencyNa entryIntercalated cellsWestern blot analysisNa concentrationTubule cellsRenal cortex