2022
Regulation of glomerulotubular balance. IV. Implication of aquaporin 1 in flow-dependent proximal tubule transport and cell volume
Du Z, Yan Q, Shen E, Weinstein A, Wang T. Regulation of glomerulotubular balance. IV. Implication of aquaporin 1 in flow-dependent proximal tubule transport and cell volume. American Journal Of Physiology. Renal Physiology 2022, 323: f642-f653. PMID: 36108052, PMCID: PMC9705020, DOI: 10.1152/ajprenal.00167.2022.Peer-Reviewed Original ResearchConceptsWater channel aquaporin-1Proximal tubulesKO miceAquaporin-1Water reabsorptionMouse PTNo significant differenceWild-typeTight junctionsRat kidneyAquaporin-1 knockoutSignificant differenceAcid-base parametersKidney proximal tubulesRenal clearance experimentsWT miceKnockout miceUrine flowFlow stimulationFluid absorptionUrine pHClearance experimentsMouse kidneyMiceRats
2020
Sex Differences in Maxi‐K channel and Klotho Expressions in ROMK Bartter’s Mouse Kidney
Kim J, Song K, Lee E, Xu S, Wang T. Sex Differences in Maxi‐K channel and Klotho Expressions in ROMK Bartter’s Mouse Kidney. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.04872.Peer-Reviewed Original ResearchMaxi-K channelsKO miceKlotho expressionMouse kidneyBartter's syndromeWT miceMaxi-KFemale WTRenal outer medullary potassium channelSurvival rateWT mouse kidneysUrinary potassium excretionProtein levelsBK channelsFemale mouse kidneyPotassium excretionVolume depletionROMKElectrolyte imbalancePotassium channelsBK expressionII phenotypeLife-threateningSex differencesBlood pressure
2017
Urinary bladder hypertrophy characteristic of male ROMK Bartter’s mice does not occur in female mice
Kim JM, Xu S, Guo X, Hu H, Dong K, Wang T. Urinary bladder hypertrophy characteristic of male ROMK Bartter’s mice does not occur in female mice. AJP Regulatory Integrative And Comparative Physiology 2017, 314: r334-r341. PMID: 29092859, PMCID: PMC5899254, DOI: 10.1152/ajpregu.00315.2017.Peer-Reviewed Original ResearchConceptsKO miceBladder hypertrophyBladder weightUrinary bladder hypertrophyRenal outer medullary potassium channelSeverity of hydronephrosisWild-type miceROMK knockout miceBladder capacityDetrusor muscleWT miceUrinary tractBartter's syndromeFemale miceSalt wastingHydronephrosisKnockout miceROMK expressionMiceBladderHypertrophyPotassium channelsMRNA levelsSignificant enlargementSyndrome
2016
Abstract P189: Disruption of Cyp2c44 Increases Sodium Chloride Cotransporter Expression and Activity in the Distal Convoluted Tubule
Su X, Wang L, Gao Z, Lin D, Pandey V, Wang T, Abraham N, Schwartzman M, Wang W. Abstract P189: Disruption of Cyp2c44 Increases Sodium Chloride Cotransporter Expression and Activity in the Distal Convoluted Tubule. Hypertension 2016, 68 DOI: 10.1161/hyp.68.suppl_1.p189.Peer-Reviewed Original ResearchNaCl cotransporterNCC expressionEpoxyeicosatrienoic acidsENaC activityRegulation of renal sodium transportFat diet-induced increaseIncreased ENaC activityAlanine-rich kinaseEffect of hydrochlorothiazideRenal sodium transportDietary Na intakeDistal convoluted tubuleCytochrome-P450Cotransporter expressionWT miceRenal expressionNCC activityKO miceNa intakeNa excretionCyp2c44Sodium transportNa transportNa absorptionENaCHydronephrosis and Urinary Bladder Hypertrophy in ROMK Bartter's Mouse
Kim J, Guo X, Hu H, Xu S, Wang T. Hydronephrosis and Urinary Bladder Hypertrophy in ROMK Bartter's Mouse. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.1224.30.Peer-Reviewed Original ResearchThickness of urotheliumKO miceROMK expressionBladder capacityBladder weightWT miceKnockout miceRenal outer medullary potassium channelLuminal membraneROMK knockout miceUrinary bladder hypertrophyLow K intakeWestern blottingLow potassium dietSmooth muscle layerMonths of ageNormal KBladder hypertrophyDetrusor muscleMouse bladderBartter's syndromePotassium dietK intakeROMKUrinary bladderExpression of KCNJ1 (ROMK) in the Gastrointestinal Tract
Han J, Lee S, Ishikawa Y, Guo X, Xu S, Wang T. Expression of KCNJ1 (ROMK) in the Gastrointestinal Tract. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.1224.31.Peer-Reviewed Original ResearchROMK expressionDistal colonMRNA expressionKO miceRenal outer medullary potassium channelEpithelial cellsGI tractGastric acid secretionGastrointestinal (GI) tractHK intakeROMK channelsWT miceDistal nephronReal-time PCRApical membraneROMKPotassium channelsAcid secretionParietal cellsIF stainingRenal tubulesQ-PCR dataEsophagusDiet animalsGastrointestinal tract
2013
REGULATION OF GLOMERULOTUBULAR BALANCE IV: IMPLICATION OF AQUAPORIN 1 IN FLOW‐DEPENDENT TRANSPORT AND CELL VOLUME
Du Z, Yan Q, Weinbaum S, Weinstein A, Wang T. REGULATION OF GLOMERULOTUBULAR BALANCE IV: IMPLICATION OF AQUAPORIN 1 IN FLOW‐DEPENDENT TRANSPORT AND CELL VOLUME. The FASEB Journal 2013, 27: 912.27-912.27. DOI: 10.1096/fasebj.27.1_supplement.912.27.Peer-Reviewed Original ResearchAQP-1Proximal tubulesKO miceWild-typeMicroperfused in vitroTransepithelial osmotic gradientHCO-3 transportWT miceNo significant differencePeritubular transportHCO-3 absorptionVolume reabsorptionPeritubular membraneWater reabsorptionAquaporin-1Mouse kidneyMicePerfusion rateH-ATPaseSignificant differenceTubulesReabsorptionOsmotic gradientCell volumePerfusionProximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion
Li H, Du Z, Barone S, Rubera I, McDonough A, Tauc M, Zahedi K, Wang T, Soleimani M. Proximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion. Journal Of Molecular Medicine 2013, 91: 951-963. PMID: 23508938, PMCID: PMC3730089, DOI: 10.1007/s00109-013-1015-3.Peer-Reviewed Original ResearchConceptsNa+/H+ exchanger NHE3Proximal convoluted tubulesKO miceMetabolic acidosisBicarbonate reabsorptionDays of acid loadingIsolated proximal convoluted tubulesConvoluted tubulesIn vitro microperfusion studyCompared to WT miceNHE3 knockout miceProximal tubule NHE3Acid loadNH4Cl acid loadMild metabolic acidosisWT miceNHE3Microperfusion studiesKnockout miceBicarbonate absorptionMutant miceVolume reabsorptionFloxed miceNH4Cl loadingTransgenic mice
2011
Chloride Channel (Clc)-5 Is Necessary for Exocytic Trafficking of Na+/H+ Exchanger 3 (NHE3)*
Lin Z, Jin S, Duan X, Wang T, Martini S, Hulamm P, Cha B, Hubbard A, Donowitz M, Guggino SE. Chloride Channel (Clc)-5 Is Necessary for Exocytic Trafficking of Na+/H+ Exchanger 3 (NHE3)*. Journal Of Biological Chemistry 2011, 286: 22833-22845. PMID: 21561868, PMCID: PMC3123051, DOI: 10.1074/jbc.m111.224998.Peer-Reviewed Original ResearchConceptsKO miceTrafficking of NHE3Proximal tubulesOpossum kidney cellsNHE3 activityDent's diseaseClC-5Surface expressionNHE3 surface expressionKidney cellsRenal proximal tubulesTotal protein levelsChloride/proton exchangerRates of basalReduced surface expressionKnockdown cellsParathyroid hormoneWT miceDegree of inhibitionCLCN5 geneSurface NHE3MiceTubule perfusionReduced expressionTwo-photon microscopyGENDER DIFFERENCE IN MODULATION OF ANGIOTENSIN II TYPE 2 RECEPTOR (AT2) FUNCTION AND EXPRESSION IN AT1A RECEPTOR‐DEFICIENT MICE
Yan Q, Wan L, Du Z, Gotoh N, Wang T. GENDER DIFFERENCE IN MODULATION OF ANGIOTENSIN II TYPE 2 RECEPTOR (AT2) FUNCTION AND EXPRESSION IN AT1A RECEPTOR‐DEFICIENT MICE. The FASEB Journal 2011, 25: 835.9-835.9. DOI: 10.1096/fasebj.25.1_supplement.835.9.Peer-Reviewed Original ResearchGlomerular filtration rateAT2 receptorsUrine volumeProximal tubulesNull miceAngiotensin II type 2 receptorAT1a-/- miceMale null miceType 2 receptorExpression levelsAT1A+/+ miceAT2 inhibitorENaC expressionAT2 expressionKidneys of maleNatriuretic effectWT miceNa+ excretionRenal functionNa+ absorptionReal-time PCRFemale null micePD123319Renal clearanceFiltration rate
2010
Identification and Regulation of Reticulon 4B (Nogo-B) in Renal Tubular Epithelial Cells
Marin EP, Moeckel G, Al-Lamki R, Bradley J, Yan Q, Wang T, Wright PL, Yu J, Sessa WC. Identification and Regulation of Reticulon 4B (Nogo-B) in Renal Tubular Epithelial Cells. American Journal Of Pathology 2010, 177: 2765-2773. PMID: 20971739, PMCID: PMC2993268, DOI: 10.2353/ajpath.2010.100199.Peer-Reviewed Original ResearchConceptsUnilateral ureteral obstructionAcute tubular necrosisEpithelial cellsRenal tubular epithelial cellsMurine kidneyIschemia/reperfusionMeasurement of fibrosisDistal nephron segmentsRecruitment of macrophagesWild-type miceInflammatory gene expressionTubular epithelial cellsDe novo expressionHuman biopsy specimensRenal injuryTubular necrosisUreteral obstructionWT miceVascular injuryHistological damageBiopsy specimensCortical tubulesDeficient miceMacrophage recruitmentTissue injury
2008
ANGIOTENSION‐INDUCED HYPERTENSION AND CHANGES IN RENAL FUNCTIONS IN MALE AND FEMALE sEH KNOCKOUT MICE
Yan Q, Raksaseri P, Weldon S, Kabcenell A, Hebert S, Wang T. ANGIOTENSION‐INDUCED HYPERTENSION AND CHANGES IN RENAL FUNCTIONS IN MALE AND FEMALE sEH KNOCKOUT MICE. The FASEB Journal 2008, 22: 941.15-941.15. DOI: 10.1096/fasebj.22.1_supplement.941.15.Peer-Reviewed Original ResearchInfusion of Ang IIFemale WT miceChronic infusion of Ang IIMale WT miceWT miceAng IIFemale KO miceKO miceChronic infusionBlood pressureMale WTMale KOMm HgAng II-induced hypertensionEffects of Ang IISEH-knockout miceAng II infusionResponse to AngII-induced hypertensionFemale wild-typeSystemic blood pressureGroups of miceWild-typeLower blood pressureSEH-knockout