2023
SLC26A1 is a major determinant of sulfate homeostasis in humans
Pfau A, López-Cayuqueo K, Scherer N, Wuttke M, Wernstedt A, Fassrainer D, Smith D, van de Kamp J, Ziegeler K, Eckardt K, Luft F, Aronson P, Köttgen A, Jentsch T, Knauf F. SLC26A1 is a major determinant of sulfate homeostasis in humans. Journal Of Clinical Investigation 2023, 133: e161849. PMID: 36719378, PMCID: PMC9888379, DOI: 10.1172/jci161849.Peer-Reviewed Original ResearchConceptsSulfate homeostasisIntervertebral disc disordersWhole-exome sequencingMajor determinantBack painPatient presentingMusculoskeletal healthDisc disordersPlasma sulfateSulfate reabsorptionFunctional expression assaysCartilage healthHomozygous mutationPotential targetPopulation studiesNumerous physiological processesRecent evidenceExome analysisHomeostasisHyposulfatemiaExpression assaysPivotal roleClinical geneticsAdditional variantsHumans
2016
N-glycosylation critically regulates function of oxalate transporter SLC26A6
Thomson RB, Thomson CL, Aronson PS. N-glycosylation critically regulates function of oxalate transporter SLC26A6. American Journal Of Physiology - Cell Physiology 2016, 311: c866-c873. PMID: 27681177, PMCID: PMC5206297, DOI: 10.1152/ajpcell.00171.2016.Peer-Reviewed Original ResearchConceptsPlasma membraneIntegral membrane proteinsCell surface deliverySLC26A6 functionTissue-specific differencesGlycosylation mutantsMembrane proteinsN-glycosylationSurface deliveryBiotinylation studiesOxalate transporterOxalate homeostasisSecond extracellular loopExtracellular loopIntact cellsEnzymatic deglycosylation studiesTransport activityEnzymatic deglycosylationFunctional studiesDeglycosylation studiesGlycosylationPutative second extracellular loopTransport functionFunctional significanceEssential role
2013
Overexpression of Pendrin in Intercalated Cells Produces Chloride-Sensitive Hypertension
Jacques T, Picard N, Miller RL, Riemondy KA, Houillier P, Sohet F, Ramakrishnan SK, Büsst CJ, Jayat M, Cornière N, Hassan H, Aronson PS, Hennings JC, Hübner CA, Nelson RD, Chambrey R, Eladari D. Overexpression of Pendrin in Intercalated Cells Produces Chloride-Sensitive Hypertension. Journal Of The American Society Of Nephrology 2013, 24: 1104-1113. PMID: 23766534, PMCID: PMC3699825, DOI: 10.1681/asn.2012080787.Peer-Reviewed Original ResearchConceptsDistal nephronTransgenic miceSame sodium intakeHigh salt intakeCause of hypertensionHigh-salt dietSodium-driven chloride/bicarbonate exchangerNet NaCl absorptionArterial hypertensionSalt intakeSodium intakeEpithelial sodium channel ENaCPathogenic roleNormal miceHypertensionRenal absorptionExchanger pendrinPrimary abnormalityVascular volumeAppropriate downregulationIntercalated cellsChloride absorptionSodium channel ENaCActivity of transportersPrimary activation
2009
Prestin's Anion Transport and Voltage-Sensing Capabilities Are Independent
Bai JP, Surguchev A, Montoya S, Aronson PS, Santos-Sacchi J, Navaratnam D. Prestin's Anion Transport and Voltage-Sensing Capabilities Are Independent. Biophysical Journal 2009, 96: 3179-3186. PMID: 19383462, PMCID: PMC2718310, DOI: 10.1016/j.bpj.2008.12.3948.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnalysis of VarianceAnimalsAnion Transport ProteinsAntiportersCarbon RadioisotopesChloridesCHO CellsCricetinaeCricetulusElectric CapacitanceFormatesGerbillinaeIon TransportMiceMutation, MissenseOxalatesPatch-Clamp TechniquesSalicylatesSulfate TransportersConceptsClosest phylogenetic relativesTransmembrane regionSLC26 anion transporter familyMammalian outer hair cellsMembrane protein prestinPrestin's motor functionAnion transportPhylogenetic relativesAnion transporter familyTransporter familyProtein prestinChloride-binding siteGating charge movementPrestinCharge movementHair cellsOuter hair cellsResiduesMechanistic conceptsVoltage sensingTransport capabilityCellsVoltage sensorPrevious observationsUptake studies
2006
Ion exchangers mediating Na+, HCO3 - and Cl- transport in the renal proximal tubule.
Aronson PS. Ion exchangers mediating Na+, HCO3 - and Cl- transport in the renal proximal tubule. Journal Of Nephrology 2006, 19 Suppl 9: s3-s10. PMID: 16736438.Commentaries, Editorials and LettersConceptsProximal tubulesNaCl absorptionApical membrane ClNull miceOxalate-sulfate exchangePendrin-null miceProximal tubule cellsBrush borderFormate exchangeRenal proximal tubulesSlc26a6-null miceAcid secretionPendrin expressionFunctional expression studiesTubule cellsNaCl reabsorptionCl absorptionBrush border membraneSulfate cotransportLines of evidenceExchanger isoformsTubulesMiceFamily membersBorder membrane
2005
Immunolocalization of anion transporter Slc26a7 in mouse kidney
Dudas PL, Mentone S, Greineder CF, Biemesderfer D, Aronson PS. Immunolocalization of anion transporter Slc26a7 in mouse kidney. American Journal Of Physiology. Renal Physiology 2005, 290: f937-f945. PMID: 16263805, DOI: 10.1152/ajprenal.00197.2004.Peer-Reviewed Original ResearchConceptsThick ascending limbMouse kidneyProximal tubulesAscending limbThick ascending limb cellsLoop of HenleProximal tubule cellsSimilar staining patternExpression of SLC26A7Recent studiesTubule cellsNull miceNephron segmentsRat kidneyKidneyDifferent epitopesStaining patternMonoclonal antibodiesAntibodiesApical membrane transportersLimb cellsCOS-7 cellsImmunofluorescence microscopyLimbBasolateral surface
2004
WNK4 regulates apical and basolateral Cl– flux in extrarenal epithelia
Kahle KT, Gimenez I, Hassan H, Wilson FH, Wong RD, Forbush B, Aronson PS, Lifton RP. WNK4 regulates apical and basolateral Cl– flux in extrarenal epithelia. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 2064-2069. PMID: 14769928, PMCID: PMC357052, DOI: 10.1073/pnas.0308434100.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCarrier ProteinsCell PolarityChloridesEpitheliumHumansImmunohistochemistryIon TransportKidneyMembrane ProteinsMembrane Transport ProteinsMiceOocytesProtein Serine-Threonine KinasesRNA, MessengerSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 2Sulfate TransportersXenopus laevisConceptsCl fluxBlood-brain barrierUnrelated ion channelsActivity of mediatorsWNK4 mRNABile ductPancreatic ductExtrarenal expressionExtrarenal tissuesCl(-) handlingPseudohypoaldosteronism type IIChannel ROMKNaCl reabsorptionSerine-threonine kinase WNK4Specialized endotheliumExchanger SLC26A6NaCl cotransporterWNK4 effectsColonic cryptsEpitheliumVariable inhibitionSweat ductsTight junctionsKidneyElectrolyte flux
2002
Ion exchangers mediating NaCl transport in the renal proximal tubule
Aronson PS. Ion exchangers mediating NaCl transport in the renal proximal tubule. Cell Biochemistry And Biophysics 2002, 36: 147-153. PMID: 12139400, DOI: 10.1385/cbb:36:2-3:147.Commentaries, Editorials and LettersConceptsProximal tubulesNaCl absorptionOxalate-sulfate exchangeLoop of HenleProximal tubule cellsNaCl transportRenal proximal tubulesRecycling of formateRole of NHE3NHE3 null miceTubule cellsNull micePerfusion solutionNephron segmentsIsoform-specific antibodiesApical membraneRenal brush border vesiclesMammalian proximal tubuleBrush border vesiclesSulfate cotransportBrush border membrane vesiclesTubulesFormate exchangeNHE3Stimulation
2001
Essential role of NHE3 in facilitating formate-dependent NaCl absorption in the proximal tubule
Wang T, Yang C, Abbiati T, Shull G, Giebisch G, Aronson P. Essential role of NHE3 in facilitating formate-dependent NaCl absorption in the proximal tubule. American Journal Of Physiology. Renal Physiology 2001, 281: f288-f292. PMID: 11457720, DOI: 10.1152/ajprenal.2001.281.2.f288.Peer-Reviewed Original Research
1997
Sodium/Hydrogen Exchanger Gene Defect in Slow-Wave Epilepsy Mutant Mice
Cox G, Lutz C, Yang C, Biemesderfer D, Bronson R, Fu A, Aronson P, Noebels J, Frankel W. Sodium/Hydrogen Exchanger Gene Defect in Slow-Wave Epilepsy Mutant Mice. Cell 1997, 91: 139-148. PMID: 9335342, DOI: 10.1016/s0092-8674(01)80016-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtaxiaBrain ChemistryCell LineCerebellumCerebral CortexChromosome MappingCrosses, GeneticElectroencephalographyEpilepsyFibroblastsGenes, RecessiveIon TransportMiceMice, Inbred C57BLMice, Neurologic MutantsOrgan SpecificityPhenotypePoint MutationRNASodiumSodium-Hydrogen ExchangersConceptsSodium/hydrogen exchangerSpontaneous mouse mutantDisease-causing mutationsPlasma membraneChromosome 4Null allelesMouse mutantsCell survivalHydrogen exchangerNHE genesMutantsGene defectsMutant miceNHE1Growth factorTonic-clonic seizuresSelective neuronal deathNeuronal deathDelicate balanceNeurological syndromeEpilepsy phenotypeGenesNew toolCell volumeMutationsIon exchangers mediating NaCl transport in the proximal tubule.
Aronson PS. Ion exchangers mediating NaCl transport in the proximal tubule. Wiener Klinische Wochenschrift 1997, 109: 435-40. PMID: 9261983.Commentaries, Editorials and LettersConceptsApical membraneBasolateral membraneBrush border membraneMembrane traffickingNHE3 protein expressionMembrane ion exchangerIntracellular vesiclesMammalian proximal tubuleBorder membraneDifferential regulationProximal tubule cellsInhibitor sensitivityPossible regulationFormate transportExchange activityProtein expressionCl- channelsTubule cellsMembranePolyclonal antibodiesVesiclesCellsNHE3Oxalate-sulfate exchangeRegulation
1996
Role of ion exchangers in mediating NaCl transport in the proximal tubule
Aronson P. Role of ion exchangers in mediating NaCl transport in the proximal tubule. Kidney International 1996, 49: 1665-1670. PMID: 8743473, DOI: 10.1038/ki.1996.243.Commentaries, Editorials and LettersConceptsProximal tubulesOxalate-sulfate exchangeProximal tubule cellsNaCl transportRecycling of formateTranscellular NaCl transportMetabolic acidosisApical membraneRenal maturationReabsorption of NaClTubule cellsNaCl reabsorptionImmunocytochemical studyNHE3 proteinBrush border membraneSulfate cotransport