2016
Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins
Jensen KH, Brandt SS, Shigematsu H, Sigworth FJ. Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins. Journal Of Structural Biology 2016, 194: 49-60. PMID: 26835990, PMCID: PMC4866491, DOI: 10.1016/j.jsb.2016.01.012.Peer-Reviewed Original Research
1999
Two-Microelectrode Voltage Clamp of Xenopus Oocytes: Voltage Errors and Compensation for Local Current Flow
Baumgartner W, Islas L, Sigworth F. Two-Microelectrode Voltage Clamp of Xenopus Oocytes: Voltage Errors and Compensation for Local Current Flow. Biophysical Journal 1999, 77: 1980-1991. PMID: 10512818, PMCID: PMC1300479, DOI: 10.1016/s0006-3495(99)77039-6.Peer-Reviewed Original Research
1993
Functional expression and purification of a homomeric human alpha 1 glycine receptor in baculovirus-infected insect cells.
Cascio M, Schoppa N, Grodzicki R, Sigworth F, Fox R. Functional expression and purification of a homomeric human alpha 1 glycine receptor in baculovirus-infected insect cells. Journal Of Biological Chemistry 1993, 268: 22135-22142. PMID: 8408073, DOI: 10.1016/s0021-9258(20)80658-9.Peer-Reviewed Original ResearchConceptsInsect cellsGlyR proteinSf9 cellsMembrane protein channelsSf9 insect cellsSodium dodecyl sulfate-polyacrylamide gelsDodecyl sulfate-polyacrylamide gelsSulfate-polyacrylamide gelsWhole cell lysatesHuman kidney cell lineShares sequenceKidney cell lineTransient expressionExpression systemStructural homologyChannel proteinsBiophysical characterizationProtein channelsFunctional channelsFunctional expressionMembrane fractionNative channelsCell surfaceGlycine receptorsChloride channels
1987
Genetic Reconstitution of Functional Acetylcholine Receptor Channels in Mouse Fibroblasts
Claudio T, Green W, Hartman D, Hayden D, Paulson H, Sigworth F, Sine S, Swedlund A. Genetic Reconstitution of Functional Acetylcholine Receptor Channels in Mouse Fibroblasts. Science 1987, 238: 1688-1694. PMID: 3686008, DOI: 10.1126/science.3686008.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCell MembraneFibroblastsGenesKineticsMiceReceptors, CholinergicTorpedoTransfectionConceptsExpression systemDNA-mediated gene transfer techniquesMouse fibroblast cellsDNA-mediated gene transferLigand-gated ion channelsCell surface AChRsNew expression systemFibroblast cellsTorpedo AChRClonal cell linesGene transfer techniquesForeign genesGene productsGenetic reconstitutionRecombinant DNACellular componentsTorpedo acetylcholine receptorComplementary DNASurface AChRsAcetylcholine receptor channelsFunctional consequencesGene transferIon channelsHomogenous cellsMouse fibroblasts
1986
Open channel noise. II. A test for coupling between current fluctuations and conformational transitions in the acetylcholine receptor
Sigworth F. Open channel noise. II. A test for coupling between current fluctuations and conformational transitions in the acetylcholine receptor. Biophysical Journal 1986, 49: 1041-1046. PMID: 2423148, PMCID: PMC1329684, DOI: 10.1016/s0006-3495(86)83732-8.Peer-Reviewed Original Research